KEGG ENZYME: 2.4.1.327

ENZYME: 2.4.1.327

Entry

EC 2.4.1.327                Enzyme

Name

aclacinomycin-T 2-deoxy-L-fucose transferase;
AknK

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

dTDP-2-deoxy-beta-L-fucose:7-(alpha-L-rhodosaminyl)aklavinone 2-deoxy-alpha-L-fucosyltransferase

Reaction(IUBMB)

dTDP-2-deoxy-beta-L-fucose + aclacinomycin T = dTDP + aclacinomycin S [RN:R09332]

Reaction(KEGG)

R09332

Substrate

dTDP-2-deoxy-beta-L-fucose [CPD:C18636];
aclacinomycin T [CPD:C18634]

Product

dTDP [CPD:C00363];
aclacinomycin S [CPD:C18635]

Comment

The enzyme, isolated from the bacterium Streptomyces galilaeus, is involved in the biosynthesis of other aclacinomycins. Also acts on idarubicin. It will slowly add a second 2-deoxy-L-fucose unit to aclacinomycin S in vitro.

History

EC 2.4.1.327 created 2014

Pathway

ec01057

Biosynthesis of type II polyketide products

ec01110

Biosynthesis of secondary metabolites

Orthology

K15948

aclacinomycin-T 2-deoxy-L-fucose transferase

Reference

1 [PMID:15078101]

Authors

Lu W, Leimkuhler C, Oberthur M, Kahne D, Walsh CT

Title

AknK is an L-2-deoxyfucosyltransferase in the biosynthesis of the anthracycline aclacinomycin A.

Journal

Biochemistry 43:4548-58 (2004)
DOI:10.1021/bi035945i

Sequence

[ag:AAF70102]

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.327

IUBMB Enzyme Nomenclature:

2.4.1.327

ExPASy - ENZYME nomenclature database:

2.4.1.327

BRENDA, the Enzyme Database:

2.4.1.327

DBGET integrated database retrieval system