KEGG   ENZYME: 2.4.1.330
Entry
EC 2.4.1.330                Enzyme                                 

Name
beta-D-glucosyl crocetin beta-1,6-glucosyltransferase;
UGT94E5;
UDP-glucose:crocetin glucosyl ester glucosyltransferasee
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-alpha-D-glucose:beta-D-glucosyl crocetin beta-1,6-glucosyltransferase
Reaction(IUBMB)
(1) UDP-alpha-D-glucose + beta-D-glucosyl crocetin = UDP + beta-D-gentiobiosyl crocetin [RN:R10988];
(2) UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin = UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin [RN:R10989];
(3) UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin = UDP + crocin [RN:R10990]
Reaction(KEGG)
Substrate
UDP-alpha-D-glucose [CPD:C00029];
beta-D-glucosyl crocetin [CPD:C19867];
bis(beta-D-glucosyl) crocetin [CPD:C19868];
beta-D-gentiobiosyl beta-D-glucosyl crocetin [CPD:C17513]
Product
UDP [CPD:C00015];
beta-D-gentiobiosyl crocetin [CPD:C19869];
beta-D-gentiobiosyl beta-D-glucosyl crocetin [CPD:C17513];
crocin [CPD:C08589]
Comment
The enzyme, characterized from the plant Gardenia jasminoides, adds a glucose to several crocetin glycosyl esters, but not to crocetin (cf. EC 2.4.1.271, crocetin glucosyltransferase).
History
EC 2.4.1.330 created 2014
Pathway
ec00999  Biosynthesis of various secondary metabolites - part 1
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K21354  beta-D-glucosyl crocetin beta-1,6-glucosyltransferase
Reference
1  [PMID:22569263]
  Authors
Nagatoshi M, Terasaka K, Owaki M, Sota M, Inukai T, Nagatsu A, Mizukami H
  Title
UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides.
  Journal
FEBS Lett 586:1055-61 (2012)
DOI:10.1016/j.febslet.2012.03.003
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.330
IUBMB Enzyme Nomenclature: 2.4.1.330
ExPASy - ENZYME nomenclature database: 2.4.1.330
BRENDA, the Enzyme Database: 2.4.1.330

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