The enzyme, isolated from the bacterium Clostridium phytofermentans, catalyses a reversible reaction. Substrates for the phosphorolytic reaction are alpha-1,3-linked oligoglucans with a polymerisation degree of 3 or more. Nigerose (i.e. 3-O-alpha-D-glucopyranosyl-D-glucopyranose) is not phosphorylyzed but can serve as substrate in the reverse direction (cf. EC 18.104.22.1689, nigerose phosphorylase).
EC 22.214.171.1244 created 2014
Nihira, T., Nishimoto, M., Nakai, H., Ohtsubo, K., and Kitaoka, M.
Characterization of two phosphorylases for alpha-1,3-oligoglucans from Clostridium phytofermentans.