KEGG   ENZYME: 2.4.1.346
Entry
EC 2.4.1.346                Enzyme                                 

Name
phosphatidyl-myo-inositol dimannoside synthase;
mannosyltransferase PimB;
PimB;
guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase (ambiguous)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
GDP-alpha-D-mannose:2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol 6-alpha-D-mannosyltransferase (configuration-retaining)
Reaction(IUBMB)
(1) GDP-alpha-D-mannose + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol = GDP + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol [RN:R11703 R12040];
(2) GDP-alpha-D-mannose + 2-O-(6-O-acyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol = GDP + 2-O-(6-O-acyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol [RN:R11704 R12041]
Reaction(KEGG)
R11703 R11704 R12040(G) R12041(G)
Substrate
GDP-alpha-D-mannose [CPD:C00096];
2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol;
2-O-(6-O-acyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol [CPD:C21609]
Product
GDP [CPD:C00035];
2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol [CPD:C21607];
2-O-(6-O-acyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol [CPD:C21608]
Comment
Requires Mg2+. The enzyme, found in Corynebacteriales, is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIMs).
History
EC 2.4.1.346 created 2017
Pathway
ec00571  Lipoarabinomannan (LAM) biosynthesis
ec01100  Metabolic pathways
Orthology
K13668  phosphatidyl-myo-inositol dimannoside synthase
Genes
KOK: KONIH1_19490
SMAR: SM39_1020
SLQ: M495_07445
PTT: VY86_07825
PSU: Psesu_0664
LCP: LC55x_3618
LGU: LG3211_3489
LEZ: GLE_0588
LEM: LEN_4346
LYT: DWG18_00865
RBD: ALSL_0430
PAT: Patl_1191
MBS: MRBBS_2200(pimB)
AAUS: EP12_13930
GPS: C427_4220
MICC: AUP74_00447(pimB_1) AUP74_02363(pimB_2)
MMAI: sS8_0454
NHL: Nhal_0611
TEE: Tel_13515
TGR: Tgr7_0793
TNI: TVNIR_0046(pimB_[H])
TBN: TBH_C0584
ENM: EBS_2101
BTD: BTI_1886
BUL: BW21_1994
HYB: Q5W_23910
RGE: RGE_04210
TBD: Tbd_0302
NIM: W01_06530
AZA: AZKH_3606
PPD: Ppro_2457
DDE: Dde_0737
DMA: DMR_34950
DGG: DGI_2231
DFL: DFE_1455
DAS: Daes_3315
PPRF: DPRO_1803
DBA: Dbac_0358
DRT: Dret_1181
MXA: MXAN_7187
CCX: COCOR_00221(pimB)
SFU: Sfum_3907
DBR: Deba_2084
PLA: Plav_1916
BRS: S23_56550
OCA: OCAR_6106
BID: Bind_3917
MSL: Msil_0984
HDN: Hden_0442
RVA: Rvan_3669
MSC: BN69_2383
PLEO: OHA_1_02180(pimB_1) OHA_1_02526(pimB_3)
RBM: TEF_03835
RMM: ROSMUCSMR3_01261(pimB)
SWI: Swit_2671
SPHD: HY78_12155
SSY: SLG_24160
ALB: AEB_P2117
ACR: Acry_1803
GXL: H845_818
RCE: RC1_4026(pimA)
MAGN: WV31_13310
PUB: SAR11_1200(amsK)
PSWU: SY83_10105
MTU: Rv2188c(pimB)
MTC: MT2243
MRA: MRA_2203
MTUR: CFBS_2316
MTD: UDA_2188c
MTUB: MT7199_2218(pimB)
MTUE: J114_11725
MTUH: I917_15355
MTUL: TBHG_02140
MTUT: HKBT1_2307
MTUU: HKBT2_2309
MBO: BQ2027_MB2211C(pimb)
MBB: BCG_2204c
MBT: JTY_2198
MBX: BCGT_2006
MAF: MAF_21990
MMIC: RN08_2426
MCX: BN42_40101(pimB)
MLE: ML0886
MLB: MLBr00886
MPA: MAP_1926c
MAO: MAP4_1902
MAVI: RC58_09490
MAVU: RE97_09495
MAV: MAV_2306
MIT: OCO_22530
MIA: OCU_22740
MID: MIP_03188
MYO: OEM_20640
MIR: OCQ_21640
MLP: MLM_1995
MUL: MUL_3543
MMC: Mmcs_3285
MKM: Mkms_3347
MJL: Mjls_3296
MMI: MMAR_3232
MMM: W7S_10710
MHAD: B586_10225
MSHG: MSG_03079(pimB)
MVA: Mvan_3550
MGI: Mflv_2961
MPHL: MPHLCCUG_03319(pimB_1)
MVQ: MYVA_3497
MTHN: 4412656_01856(pimB)
MHAS: MHAS_01520(pimB)
MDU: MDUV_08810(pimB)
MAB: MAB_1976
MABB: MASS_1965
MCHE: BB28_10275
MSTE: MSTE_01914
MSAL: DSM43276_01770(pimB_2)
MTER: 4434518_01643(pimB_2)
ASD: AS9A_1291
CGL: NCgl2106(Cgl2186)
CGB: cg2400
CGU: WA5_2106(PimB')
CGT: cgR_2068
CGM: cgp_2400
CGJ: AR0_10390
CEF: CE2079
CDI: DIP1620
CDP: CD241_1556(pimB')
CDH: CDB402_1524(pimB')
CDT: CDHC01_1557(pimB')
CDE: CDHC02_1505(pimB')
CDR: CDHC03_1532(pimB')
CDA: CDHC04_1532(pimB')
CDZ: CD31A_1635(pimB')
CDB: CDBH8_1606(pimB')
CDS: CDC7B_1618(pimB')
CDD: CDCE8392_1527(pimB')
CDW: CDPW8_1610(pimB')
CDV: CDVA01_1493(pimB')
CDIP: ERS451417_01644(pimB')
CJK: jk0727
CUR: cu1229(pimB')
CUA: CU7111_1211(pimB')
CAR: cauri_1693(pimB')
CKP: ckrop_0718(pimB')
CPL: Cp3995_1456(pimB)
CPP: CpP54B96_1439(pimB)
CPZ: CpPAT10_1414(pimB)
COR: Cp267_1475(pimB)
COD: Cp106_1399(pimB)
COS: Cp4202_1406(pimB)
CPSE: CPTA_02003
CPSU: CPTB_01758
CPSF: CPTC_01998
CRD: CRES_0768(pimB1)
CUL: CULC22_01536(pimB2)
CUC: CULC809_01520(pim)
CUE: CULC0102_1654(pimB2)
CUN: Cul210932_1606(pimB2)
CUS: CulFRC11_1508(pimB2)
CUQ: Cul210931_1491(pimB2)
CUZ: Cul05146_1578(pimB2)
CUJ: CUL131002_1528c(pimB2)
CVA: CVAR_1139(pimBP)
CTER: A606_04550
CGY: CGLY_09830(pimB)
COA: DR71_315(pimB)
CSX: CSING_09190(pimB)
CMQ: B840_08460(pimB)
CKU: UL82_07095(pimB)
CCJ: UL81_07930(pimB)
CMV: CMUST_10975(pimB)
CEI: CEPID_08470(pimB)
CTED: CTEST_09050(pimB)
CUT: CUTER_07500(pimB)
CDX: CDES_09915(pimB)
CSP: WM42_2257(pimB)
CPHO: CPHO_04125
CGV: CGLAU_08495(pimB1)
CAQU: CAQU_08580
CAMG: CAMM_04930
CMIN: NCTC10288_00789(pimB')
CPEG: CPELA_03685(pimB2)
CEE: CENDO_07750(pimB1)
NFA: NFA_17180
NFR: ERS450000_02396(pimB_1)
NNO: NONO_c23330(pimB)
RER: RER_35870(pimB')
REY: O5Y_16435
ROP: ROP_08500(pimB')
REQ: REQ_28150
RHB: NY08_666
RFA: A3L23_03917(pimB_2) A3L23_05001(pimB_3)
RHS: A3Q41_04330(pimB_2)
RHU: A3Q40_01182(pimB_1)
RRT: 4535765_02999(pimB')
GBR: Gbro_3047
GPO: GPOL_c28050(pimB)
GOR: KTR9_3037
GRU: GCWB2_15800(pimB1)
GOM: D7316_00242(pimB_1)
TPR: Tpau_2681
SRT: Srot_1065
SCO: SCO2132(SC6G10.05c) SCO5681(SC5H4.05c)
SCT: SCAT_1260
SFA: Sfla_4686
SHY: SHJG_3616
SVE: SVEN_1792
SDV: BN159_6301(pimB)
SALS: SLNWT_5748
STRP: F750_1996
SFI: SFUL_1706
SALU: DC74_2617
SALL: SAZ_14405
SLV: SLIV_10220(pimB1) SLIV_27045(pimB2)
SGU: SGLAU_09720(pimB)
STRE: GZL_03111
STRM: M444_10925
SAMB: SAM23877_2229(pimB)
SPRI: SPRI_5367
SRW: TUE45_02663(pimB_1) TUE45_06404(pimB_3)
SLE: sle_50070(sle_50070)
SRN: A4G23_01321(pimB)
STRD: NI25_28515
SMAL: SMALA_2066
SLAU: SLA_1726
SALF: SMD44_02339(pimB)
SALJ: SMD11_4923(pimB)
SLX: SLAV_26535(pimB3)
SGE: DWG14_06197(pimB_2)
SRJ: SRO_5328
LMOI: VV02_14375
XCE: Xcel_2461
IDO: I598_0210(pimB_1)
CFL: Cfla_2358
SERJ: SGUI_2442
MPH: MLP_41690
KFL: Kfla_2803
TFU: Tfu_1764
NDA: Ndas_3146
NAL: B005_0109(pimB)
STRR: EKD16_10065(pimB5) EKD16_11955(pimB6)
TCU: Tcur_3059
SRO: Sros_2695
ACE: Acel_0972
NML: Namu_3236
MMAR: MODMU_3521
SACC: EYD13_05880(pimB1)
AMD: AMED_2210
AMN: RAM_11255
AMM: AMES_2188
AMZ: B737_2189
AOI: AORI_5713
AJA: AJAP_10895(pimB)
AMYY: YIM_12415(pimB2)
PSEA: WY02_06760
PSEE: FRP1_07235
PSEH: XF36_08040
PAUT: Pdca_43360
AMI: Amir_1382
SESP: BN6_16340(gtuS4-3)
KAL: KALB_1982
ACTI: UA75_25455
ACAD: UA74_24875
AHG: AHOG_22845(pimB)
ALO: CRK55704
SAQ: Sare_3514
MIL: ML5_3750
ASE: ACPL_1663
AFS: AFR_09490
ACTS: ACWT_1541
CAI: Caci_6075
SNA: Snas_4876
AHE: Arch_1096
TBW: NCTC13354_01752(pimB)
AHW: NCTC11636_00090(pimB_1)
TBI: Tbis_1304
RXY: Rxyl_0383
AFO: Afer_0937
SYZ: MYO_13560
SYY: SYNGTS_0352(slr1076)
SYT: SYNGTI_0352(slr1076)
SYS: SYNPCCN_0352(slr1076)
SYQ: SYNPCCP_0352(slr1076)
LBO: LBWT_1050
AMR: AM1_4019
TER: Tery_1135
NAZ: Aazo_3845
CALH: IJ00_19330
HAU: Haur_3826
ATM: ANT_26130
OTE: Oter_4017
OBG: Verru16b_01985(pimB_1) Verru16b_02842(pimB_5)
RUL: UC8_45400(pimB_6)
MFF: MFFC18_29150(pimB_3)
PLS: VT03_02590(pimB_1)
IPA: Isop_1080
SACI: Sinac_5387
AGV: OJF2_27560(pimB_1)
ABAS: ACPOL_0782
ABAC: LuPra_01058(pimB_2) LuPra_03303(pimB_4)
BCEL: BcellWH2_00189(pimB_1)
SRM: SRM_00357
PHE: Phep_3893
MUC: MuYL_4925
CHU: CHU_0894
CCH: Cag_1473
CLI: Clim_0285
PVI: Cvib_0682
PROC: Ptc2401_00359(pimB_2)
CTS: Ctha_0296
NDE: NIDE0739
TON: TON_1857
MEMA: MMAB1_1830
HWA: HQ_3544A(gth9)
HWC: Hqrw_3023(gth10)
NAT: NJ7G_3847
 » show all
Reference
1  [PMID:19638342]
  Authors
Guerin ME, Kaur D, Somashekar BS, Gibbs S, Gest P, Chatterjee D, Brennan PJ, Jackson M
  Title
New insights into the early steps of phosphatidylinositol mannoside biosynthesis  in mycobacteria: PimB' is an essential enzyme of Mycobacterium smegmatis.
  Journal
J Biol Chem 284:25687-96 (2009)
DOI:10.1074/jbc.M109.030593
  Sequence
Reference
2  [PMID:19395496]
  Authors
Mishra AK, Batt S, Krumbach K, Eggeling L, Besra GS
  Title
Characterization of the Corynebacterium glutamicum deltapimB' deltamgtA double deletion mutant and the role of Mycobacterium tuberculosis orthologues Rv2188c and Rv0557 in glycolipid biosynthesis.
  Journal
J Bacteriol 191:4465-72 (2009)
DOI:10.1128/JB.01729-08
  Sequence
[mtu:Rv2188c]
Reference
3  [PMID:20843801]
  Authors
Batt SM, Jabeen T, Mishra AK, Veerapen N, Krumbach K, Eggeling L, Besra GS, Futterer K
  Title
Acceptor substrate discrimination in phosphatidyl-myo-inositol mannoside synthesis: structural and mutational analysis of mannosyltransferase Corynebacterium glutamicum PimB'.
  Journal
J Biol Chem 285:37741-52 (2010)
DOI:10.1074/jbc.M110.165407
  Sequence
[cgl:NCgl2106]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.346
IUBMB Enzyme Nomenclature: 2.4.1.346
ExPASy - ENZYME nomenclature database: 2.4.1.346
BRENDA, the Enzyme Database: 2.4.1.346

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