KEGG   ENZYME: 2.4.1.349
Entry
EC 2.4.1.349                Enzyme                                 

Name
mannosyl-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol 3-alpha-mannosyltransferase;
WbdB
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
GDP-alpha-D-mannose:alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol 3-alpha-mannosyltransferase (configuration-retaining)
Reaction(IUBMB)
2 GDP-alpha-D-mannose + alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = 2 GDP + alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol [RN:R11938]
Reaction(KEGG)
R11938
Substrate
GDP-alpha-D-mannose [CPD:C00096];
alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol [CPD:C21780]
Product
GDP [CPD:C00035];
alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol [CPD:C21781]
Comment
The enzyme is involved in the biosynthesis of the linker region of the polymannose O-polysaccharide in the outer leaflet of the membrane of Escherichia coli serotypes O8, O9 and O9a. It has no activity with N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol (cf. EC 2.4.1.348, N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol 3-alpha-mannosyltransferase).
History
EC 2.4.1.349 created 2017
Orthology
K12994  mannosyl-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol 3-alpha-mannosyltransferase / alpha-1,3-rhamnosyltransferase
Genes
ECX: EcHS_A2167(mtfB)
ECR: ECIAI1_2098(wbdB)
SBG: SBG_0617(wbdB)
SBZ: A464_690
SBV: N643_03110
KPJ: N559_1773
KVA: Kvar_1571
KPE: KPK_1681
KVD: KR75_26275
KVQ: SP68_06420
EAR: CCG29191
AHN: NCTC12129_03344(wbdB)
YAL: AT01_3985
YIN: CH53_3321
YRO: CH64_56
SMW: SMWW4_v1c15890(wbpY)
SPLY: Q5A_007990
SFJ: SAMEA4384070_1648(glgA_1)
SGL: SG1120
SOD: Sant_1472(rbfU)
PES: SOPEG_1958(rbfU)
PAM: PANA_2483(rfbU)
AAN: D7S_01298
XAC: XAC0041(wbdB)
XCI: XCAW_00426(wbdB)
DKO: I596_3631
PAE: PA5448(wbpY)
PAEV: N297_5636
PAEI: N296_5636
PAU: PA14_71920(wbpY)
PAP: PSPA7_6238(wbpY)
PAG: PLES_58431(wbpY)
PAF: PAM18_5570(wbpY)
PNC: NCGM2_6225(wbpY)
PAEB: NCGM1900_6303(wbpY)
PAEP: PA1S_29430
PAEM: U769_29935
PAEL: T223_29895
PAEU: BN889_06055(wbpY)
PAEG: AI22_04555
PAEC: M802_5634
PAEO: M801_5501
PCQ: PcP3B5_01680(mshA_1)
PPU: PP_1801(wbpY)
PPT: PPS_1443
PPI: YSA_02162
PPX: T1E_3060
PPUT: L483_06560
PPUD: DW66_1723
PFE: PSF113_5891(wbpY)
PMAN: OU5_3472
PKC: PKB_5495
PSES: PSCI_5332
PSEM: TO66_31525
SVO: SVI_1562
SPSW: Sps_03620
PSEO: OM33_05170
MICC: AUP74_02368(mshA_2)
MICT: FIU95_08640(mshA4)
RVI: RVIR1_05430(wbpY)
TMC: LMI_3104
MMAI: sS8_0775
FNA: OOM_1625
MEJ: Q7A_1108
MEC: Q7C_2313
NOC: Noc_0732
NWA: Nwat_2380
SLIM: SCL_1224
RPI: Rpic_1156
REH: H16_A2898(h16_A2898)
CGD: CR3_0500(rfaG)
BTE: BTH_I1333
BTQ: BTQ_2601
BTJ: BTJ_3094
BTZ: BTL_1024
BTD: BTI_804
BTV: BTHA_1117
BTHE: BTN_363
BTHM: BTRA_1232
BTHL: BG87_1221
BOK: DM82_2468
BOC: BG90_2266
BVE: AK36_5110
BCEN: DM39_692
BCEO: I35_0750
BCT: GEM_2643
BCED: DM42_944
BTEI: WS51_14700
BSEM: WJ12_18285
BGU: KS03_1587
BGO: BM43_2090
BUK: MYA_5167
BUL: BW21_900
BGP: BGL_1c06380(wbpY)
CABA: SBC2_28770(mshA_16)
PUT: PT7_3167
AMIM: MIM_c06920
HYB: Q5W_14060
CFU: CFU_1122(wbpY)
CARE: LT85_3688
LCH: Lcho_0975
MFA: Mfla_1273
NIM: W01_08530
HMS: HMU09730
HCE: HCW_04660
CFZ: CSG_6180
SUN: SUN_0344
NIS: NIS_1384
GLO: Glov_1956
GEM: GM21_2534
GEB: GM18_1503
DBA: Dbac_2511
DRT: Dret_0377
DAL: Dalk_2220
MXA: MXAN_2920
RLG: Rleg_0456
NGL: RG1141_PA05550(wbpY)
BMEL: DK63_1
BMEE: DK62_873
BABO: DK55_559
BABR: DO74_1328
BABT: DK49_322
BABB: DK48_1557
BABU: DK53_549
BABS: DK51_911
BABC: DO78_466
BSZ: DK67_1456
BOV: BOV_0533
BCAR: DK60_610
BCAS: DA85_02520
BMR: BMI_I531
BPP: BPI_I560
BPV: DK65_831
BBT: BBta_1074
BRS: S23_22610
RPC: RPC_4200
RPE: RPE_4250
RPT: Rpal_3765
NHA: Nham_2764
OCA: OCAR_6112
XAU: Xaut_3559
AZC: AZC_0046
SNO: Snov_1766
MOR: MOC_6051
BID: Bind_2650
MSL: Msil_2724
RVA: Rvan_2465
PLEO: OHA_1_03373(mshA_7)
HDI: HDIA_4822(epsD)
CAK: Caul_2078
JAN: Jann_2029
DSH: Dshi_3578
CID: P73_0708
MALG: MALG_03185
ZMO: ZMO1232
ZMN: Za10_0099
ZMB: ZZ6_0100
ZMI: ZCP4_0098
ZMC: A265_00098(mshA)
ZMR: A254_00098(mshA)
SECH: B18_08515
SSY: SLG_10220
SPHR: BSY17_2950
SINB: SIDU_12415
ALB: AEB_P0684
GDI: GDI2412
GDJ: Gdia_0663
KEU: S101446_02054(wbyK)
KSC: CD178_02547(mshA_1) CD178_02685(mshA_2)
MGRY: MSR1_30980(mfpsA_2)
MAGN: WV31_13340
BMX: BMS_2396
ACU: Atc_0210
ATX: GCD22_00848(wbbL) GCD22_00850(rfbU)
GKA: GK3308
BBE: BBR47_17510(mtfB)
PLV: ERIC2_c01960(wscE)
CAE: SMB_G2943(rfbU) SMB_G2947
CPF: CPF_2483(mtfB) CPF_2487
CPR: CPR_2193(mtfB) CPR_2197
CBM: CBF_0141
CTYK: CTK_C01130
CCOH: SAMEA4530647_0128(mshA_1) SAMEA4530647_0132(mshA_2)
HHW: NCTC503_02295(mfpsA) NCTC503_02299(mshA_2)
CTHM: CFE_1250
MANA: MAMMFC1_02313(mshA_3) MAMMFC1_02321(mshA_5) MAMMFC1_02325(mfpsA)
STED: SPTER_34630(mshA_2)
MMC: Mmcs_3586
MKM: Mkms_3659
MJL: Mjls_3591
MVQ: MYVA_5174
CGT: cgR_0034
NFR: ERS450000_06111(mfpsA_2)
NAD: NCTC11293_03704(mshA_3)
RER: RER_42910
REY: O5Y_20100
ROP: ROP_54790
REQ: REQ_40630
RHB: NY08_332
RFA: A3L23_01242 A3L23_03657(mshA_5)
RHS: A3Q41_04583(mshA_4)
RHU: A3Q40_02599(mshA_3)
RRT: 4535765_04147(mshA_3)
GOR: KTR9_3662
GRU: GCWB2_12785(mshA2)
GOM: D7316_04738(glgM_1)
LXX: Lxx04890
CMI: CMM_0718(wcnE) CMM_1006(wcqB) CMM_1712
CMC: CMN_00668(wcnE) CMN_00969(wcqB) CMN_01692
MOO: BWL13_00218(mshA_1)
MLV: CVS47_00748(mfpsA)
AMIN: AUMI_11840
ACH: Achl_1991
XCE: Xcel_2903
IDO: I598_3216(pimA_2)
CFI: Celf_3046
BLIN: BLSMQ_1818
ACIJ: JS278_02724(mshA_2)
PSIM: KR76_10940
SRO: Sros_1373
MMAR: MODMU_0474
SACC: EYD13_03420(tuaC) EYD13_03435(pimA1) EYD13_03440(mshA3)
AMN: RAM_05155
AMM: AMES_1009
AMZ: B737_1010
AOI: AORI_0993(wbpY) AORI_0996
AMYY: YIM_42605(mshA5) YIM_42610(pimA3) YIM_42625(tuaC)
PSEA: WY02_10645
PSEH: XF36_19230
PAUT: Pdca_54310
SESP: BN6_76310(gtuS4-15) BN6_76330(gtuS4-16) BN6_76370(gtuS4-17)
AHG: AHOG_24555(mshA5) AHOG_24560(pimA2) AHOG_24575(tuaC)
SAQ: Sare_0881
MIL: ML5_1194
ASE: ACPL_1043
AMS: AMIS_8500
AFS: AFR_05630
ACTS: ACWT_0925
SNA: Snas_6016
AIR: NCTC12972_00197(mfpsA) NCTC12972_00198(mshA)
RXY: Rxyl_0690
SYZ: MYO_115220(mtfB)
SYY: SYNGTS_1509(mtfB)
SYT: SYNGTI_1509(mtfB)
SYS: SYNPCCN_1508(mtfB)
SYQ: SYNPCCP_1508(mtfB)
MAR: MAE_15270
CYT: cce_0040(mtfB)
GLJ: GKIL_3344
NAZ: Aazo_5201
CALH: IJ00_06230
DOU: BMF77_03112(mfpsA_1) BMF77_03113(mfpsA_2) BMF77_03749(mshA_6)
CEO: ETSB_0819(mtfB)
CER: RGRSB_0775(mtfB)
AGL: PYTT_0430
PIR: VN12_04540(mgs)
RUL: UC8_45380(mfpsA_1)
MFF: MFFC18_01450(pimB_1) MFFC18_22060(rfaG)
TTF: THTE_2364
PLH: VT85_02830(mshA_1) VT85_18125(glgA_1) VT85_20630(mshA_5)
BVO: Pan97_36980(mshA_3)
GES: VT84_19585(mshA_5)
AGV: OJF2_07350(mshA_1) OJF2_54900(mshA_6)
PBU: L21SP3_00744(pglJ)
PBP: STSP1_00213(mshA)
TAZ: TREAZ_0744(mtfA)
LIE: LIF_A1888
LIC: LIC_11627(mtf)
LIS: LIL_11747
LST: LSS_12539
ABAS: ACPOL_4407
ABAC: LuPra_00528(kanF_1) LuPra_03613(mshA_3) LuPra_03838(mshA_4) LuPra_03841(mshA_5)
LSZ: JCM16776_1922(mshA)
GAU: GAU_0662
BXY: BXY_26670
BOA: Bovatus_03500(pglJ_2)
BVU: BVU_1728
PGI: PG_0129
PGN: PGN_0242
PCRE: NCTC12858_00592(mfpsA)
TFO: BFO_0475
PRU: PRU_2900
DORI: FH5T_06740
SRM: SRM_00258(rfaG) SRM_00262(rfaG) SRM_p84031(rfaG)
SGN: SGRA_1777
MUC: MuYL_4263
LBY: Lbys_0649
HSW: Hsw_1445
GFO: GFO_0552
FJO: Fjoh_2517
FIN: KQS_04730
COC: Coch_0625
KOS: KORDIASMS9_02544(mfpsA)
KAN: IMCC3317_43670(mfpsA)
MARF: CJ739_2998
RAG: B739_1930
RAT: M949_0137
ELB: VO54_02338(glgA_2)
CHZ: CHSO_2470
CTS: Ctha_0386
IAL: IALB_0742
CPRV: CYPRO_1586
NJA: NSJP_0001
MMD: GYY_01895
MAE: Maeo_0394
MFC: BRM9_1105
CDIV: CPM_1034
TNU: BD01_1491
MBAR: MSBR2_2672
MBAK: MSBR3_2753
MAC: MA_3757
MMAC: MSMAC_0661
MTHR: MSTHT_2369
MTHE: MSTHC_0911
MMET: MCMEM_2094
TPE: Tpen_1744
 » show all
Reference
1  [PMID:22875852]
  Authors
Greenfield LK, Richards MR, Li J, Wakarchuk WW, Lowary TL, Whitfield C
  Title
Biosynthesis of the polymannose lipopolysaccharide O-antigens from Escherichia coli serotypes O8 and O9a requires a unique combination of single- and multiple-active site mannosyltransferases.
  Journal
J Biol Chem 287:35078-91 (2012)
DOI:10.1074/jbc.M112.401000
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.349
IUBMB Enzyme Nomenclature: 2.4.1.349
ExPASy - ENZYME nomenclature database: 2.4.1.349
BRENDA, the Enzyme Database: 2.4.1.349

DBGET integrated database retrieval system