KEGG   ENZYME: 2.4.1.360Help
Entry
EC 2.4.1.360                Enzyme                                 

Name
2-hydroxyflavanone C-glucosyltransferase;
OsCGT
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-glucose:2'-hydroxy-beta-oxodihydrochalcone C6/8-beta-D-glucosyltransferase
Reaction(IUBMB)
UDP-alpha-D-glucose + a 2'-hydroxy-beta-oxodihydrochalcone = UDP + a 3'-(beta-D-glucopyranosyl)-2'-hydroxy-beta-oxodihydrochalcone [RN:R12290]
Reaction(KEGG)
Substrate
UDP-alpha-D-glucose [CPD:C00029];
2'-hydroxy-beta-oxodihydrochalcone [CPD:C22066]
Product
UDP [CPD:C00015];
3'-(beta-D-glucopyranosyl)-2'-hydroxy-beta-oxodihydrochalcone [CPD:C22067]
Comment
The enzyme has been characterized in Oryza sativa (rice), various Citrus spp., Glycine max (soybean), and Fagopyrum esculentum (buckwheat). Flavanone substrates require a 2-hydroxy group. The meta-stable flavanone substrates such as 2-hydroxynaringenin exist in an equilibrium with open forms such as 1-(4-hydroxyphenyl)-3-(2,4,6-trihydroxyphenyl)propane-1,3-dione, which are the actual substrates for the glucosyl-transfer reaction (see EC 1.14.14.162, flavanone 2-hydroxylase). The enzyme can also act on dihydrochalcones. The enzymes from citrus plants can catalyse a second C-glycosylation reaction at position 5.
History
EC 2.4.1.360 created 2018
Orthology
K23154  2-hydroxyflavanone C-glucosyltransferase
Genes
CIT: 102616213 102618156
CIC: CICLE_v10013670mg CICLE_v10031419mg
TCC: 18601595 18610301 18610303 18610304
GRA: 105780808
GHI: 107893931 107897380 107920122
GAB: 108459103 108462068
DZI: 111288214 111292515
EGR: 104423661 104442902 104445237 104445238 104447177 104447179 104447180 104447181 104447183
GMX: 100776116(UGT708D1) 100816555
PVU: PHAVU_004G137900g PHAVU_004G1381000 PHAVU_004G138200g PHAVU_004G1384000 PHAVU_004G1384001
CAM: 101494394(UGT708B1)
LJA: Lj2g3v0604430.1(Lj2g3v0604430.1) Lj2g3v0604440.1(Lj2g3v0604440.1) Lj2g3v1267160.1(Lj2g3v1267160.1)
ADU: 107489286
CSV: 101222215
CMO: 103489166
MCHA: 111019065
CMAX: 111482455
CMOS: 111458784
CPEP: 111777626
RCU: 8281269
JRE: 109003122
NSY: 104241134
NTO: 104092631
INI: 109180632
OEU: 111400904
CCAV: 112513046
SOE: 110796490
CQI: 110735058
PSOM: 113346508
DOSA: Os06t0288300-01(Os06g0288300) Os06t0289200-01(Os06g0289200) Os06t0289900-01(Os06g0289900)
ATS: 109732191(LOC109732191) 109732196(LOC109732196) 109764127(LOC109764127) 109764128(LOC109764128) 109782690(LOC109782690)
MUS: 103974397
AOF: 109849013
ATR: 18438457
 » show all
Taxonomy
Reference
1  [PMID:19411659]
  Authors
Brazier-Hicks M, Evans KM, Gershater MC, Puschmann H, Steel PG, Edwards R
  Title
The C-glycosylation of flavonoids in cereals.
  Journal
J Biol Chem 284:17926-34 (2009)
DOI:10.1074/jbc.M109.009258
  Sequence
[osa:9266253]
Reference
2  [PMID:25142187]
  Authors
Nagatomo Y, Usui S, Ito T, Kato A, Shimosaka M, Taguchi G
  Title
Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon.
  Journal
Plant J 80:437-48 (2014)
DOI:10.1111/tpj.12645
  Sequence
Reference
3  [PMID:25979175]
  Authors
Hirade Y, Kotoku N, Terasaka K, Saijo-Hamano Y, Fukumoto A, Mizukami H
  Title
Identification and functional analysis of 2-hydroxyflavanone C-glucosyltransferase in soybean (Glycine max).
  Journal
FEBS Lett 589:1778-86 (2015)
DOI:10.1016/j.febslet.2015.05.010
  Sequence
[gmx:100776116]
Reference
4  [PMID:28370711]
  Authors
Ito T, Fujimoto S, Suito F, Shimosaka M, Taguchi G
  Title
C-Glycosyltransferases catalyzing the formation of di-C-glucosyl flavonoids in citrus plants.
  Journal
Plant J 91:187-198 (2017)
DOI:10.1111/tpj.13555
  Sequence
[cit:102618156]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.360
IUBMB Enzyme Nomenclature: 2.4.1.360
ExPASy - ENZYME nomenclature database: 2.4.1.360
BRENDA, the Enzyme Database: 2.4.1.360

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