KEGG   ENZYME: 2.4.1.363
Entry
EC 2.4.1.363                Enzyme                                 

Name
ginsenoside 20-O-glucosyltransferase;
UGT71A27 (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-alpha-D-glucose:(20S)-protopanaxadiol 20-O-glucosyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-alpha-D-glucose + (20S)-protopanaxadiol = UDP + ginsenoside C-K [RN:R12382]
Reaction(KEGG)
R12382
Substrate
UDP-alpha-D-glucose [CPD:C00029];
(20S)-protopanaxadiol [CPD:C20715]
Product
UDP [CPD:C00015];
ginsenoside C-K [CPD:C22127]
Comment
The enzyme, characterized from the plant Panax ginseng, transfers a glucosyl moiety to the free C20(S)-OH group of dammarane derivative substrates, including protopanaxatriol, dammarenediol II, (20S)-ginsenoside Rh2, and (20S)-ginsenoside Rg3. It does not act on the 20R epimer of protopanaxadiol, or on ginsenosides that are glucosylated at the C-6 position, such as ginsenoside Rh1 or ginsenoside Rg2.
History
EC 2.4.1.363 created 2019
Orthology
K23520  ginsenoside 20-O-glucosyltransferase
Reference
1  [PMID:24603359]
  Authors
Yan X, Fan Y, Wei W, Wang P, Liu Q, Wei Y, Zhang L, Zhao G, Yue J, Zhou Z
  Title
Production of bioactive ginsenoside compound K in metabolically engineered yeast.
  Journal
Cell Res 24:770-3 (2014)
DOI:10.1038/cr.2014.28
  Sequence
Reference
2  [PMID:26032089]
  Authors
Wei W, Wang P, Wei Y, Liu Q, Yang C, Zhao G, Yue J, Yan X, Zhou Z
  Title
Characterization of Panax ginseng UDP-Glycosyltransferases Catalyzing Protopanaxatriol and Biosyntheses of Bioactive Ginsenosides F1 and Rh1 in Metabolically Engineered Yeasts.
  Journal
Mol Plant 8:1412-24 (2015)
DOI:10.1016/j.molp.2015.05.010
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.363
IUBMB Enzyme Nomenclature: 2.4.1.363
ExPASy - ENZYME nomenclature database: 2.4.1.363
BRENDA, the Enzyme Database: 2.4.1.363

DBGET integrated database retrieval system