KEGG   ENZYME: 2.4.1.370
Entry
EC 2.4.1.370                Enzyme                                 

Name
inositol phosphorylceramide mannosyltransferase;
SUR1 (gene name);
CSH1 (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
GDP-alpha-D-mannose:(4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-[(1D-myo-inositol-1-O-yl)hydroxyphosphoryl]sphinganine mannosyltransferase (configuration-retaining)
Reaction(IUBMB)
GDP-alpha-D-mannose + a (4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-[(1D-myo-inositol-1-O-yl)hydroxyphosphoryl]sphinganine = a (4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-{[6-O-(alpha-D-mannosyl)-1D-myo-inositol-1-O-yl]hydroxyphosphoryl}sphinganine + GDP [RN:R12472]
Reaction(KEGG)
R12472
Substrate
GDP-alpha-D-mannose [CPD:C00096];
(4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-[(1D-myo-inositol-1-O-yl)hydroxyphosphoryl]sphinganine
Product
(4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-{[6-O-(alpha-D-mannosyl)-1D-myo-inositol-1-O-yl]hydroxyphosphoryl}sphinganine;
GDP [CPD:C00035]
Comment
The simplest complex sphingolipid of yeast, inositol-phospho-alpha-hydroxyphytoceramide (IPC), is usually mannosylated to yield mannosyl-inositol-phospho-alpha hydroxyphytoceramide (MIPC). The enzyme is located in the Golgi apparatus, and utilizes GDP-mannose as the mannosyl group donor. It consists of a catalytic subunit (SUR1 or CSH1) and a regulatory subunit (CSG2).
History
EC 2.4.1.370 created 2019
Orthology
K22721  inositol phosphorylceramide mannosyltransferase catalytic subunit
Genes
HRO: HELRODRAFT_193660 HELRODRAFT_85985
LGI: LOTGIDRAFT_165932 LOTGIDRAFT_234732
PCAN: 112554310 112554653
CRG: 105322441
MYI: 110451184
OBI: 106875328
LAK: 106151176 106153359 106161453 106169507 106173083 106173093 106180932 106181098
QSU: 111991165 111992762 112001261 112011196 112011282 112021804
MNG: MNEG_2602 MNEG_8864
CSL: COCSUDRAFT_38902 COCSUDRAFT_57606 COCSUDRAFT_62728 COCSUDRAFT_65781 COCSUDRAFT_66191
SCE: YBR161W(CSH1) YPL057C(SUR1)
KMX: KLMA_30359(SUR1)
NCS: NCAS_0B02330(NCAS0B02330) NCAS_0C02270(NCAS0C02270)
NDI: NDAI_0C05350(NDAI0C05350) NDAI_0E02960(NDAI0E02960)
TPF: TPHA_0I02040(TPHA0I02040) TPHA_0L01480(TPHA0L01480)
TBL: TBLA_0A00490(TBLA0A00490) TBLA_0H03450(TBLA0H03450)
TDL: TDEL_0B01240(TDEL0B01240)
KAF: KAFR_0A01170(KAFR0A01170) KAFR_0E03040(KAFR0E03040)
CAL: CAALFM_C209150WA(MIT1)
SLB: AWJ20_3499(SUR1)
NCR: NCU07761
NTE: NEUTE1DRAFT109478(NEUTE1DRAFT_109478) NEUTE1DRAFT59032(NEUTE1DRAFT_59032)
MBE: MBM_03773
ANI: AN6534.2
ANG: ANI_1_120134(An15g00630) ANI_1_924084(An09g00100)
PNO: SNOG_05811 SNOG_09174(SNOG_09175)
PTE: PTT_16724
SPO: SPAC17G8.11c(imt3) SPAC2F3.01(imt1) SPCC4F11.04c(imt2)
CNE: CNH00360
CNB: CNBL0340
TASA: A1Q1_02468
ABP: AGABI1DRAFT91417(AGABI1DRAFT_91417)
ABV: AGABI2DRAFT142533(AGABI2DRAFT_142533)
MGL: MGL_0392
MRT: MRET_2213
MSYM: MSY001_3200(OCH1)
SMIN: v1.2.010590.t1(symbB.v1.2.010590.t1) v1.2.015706.t1(symbB.v1.2.015706.t1) v1.2.025490.t1(symbB.v1.2.025490.t1) v1.2.025490.t2(symbB.v1.2.025490.t2) v1.2.037975.t1(symbB.v1.2.037975.t1) v1.2.037975.t2(symbB.v1.2.037975.t2)
SPAR: SPRG_08368
BOK: DM82_1689
BOC: BG90_3021
AVI: Avi_3933
SHZ: shn_26300
HDI: HDIA_3838
ZMN: Za10_1038
RBA: RB9614(sur1)
PHE: Phep_0687
FBA: FIC_00838
 » show all
Reference
1  [PMID:9323360]
  Authors
Beeler TJ, Fu D, Rivera J, Monaghan E, Gable K, Dunn TM
  Title
SUR1 (CSG1/BCL21), a gene necessary for growth of Saccharomyces cerevisiae in the presence of high Ca2+ concentrations at 37 degrees C, is required for mannosylation of inositolphosphorylceramide.
  Journal
Mol Gen Genet 255:570-9 (1997)
DOI:10.1007/s004380050530
  Sequence
Reference
2  [PMID:9395539]
  Authors
Dean N, Zhang YB, Poster JB
  Title
The VRG4 gene is required for GDP-mannose transport into the lumen of the Golgi in the yeast, Saccharomyces cerevisiae.
  Journal
J Biol Chem 272:31908-14 (1997)
DOI:10.1074/jbc.272.50.31908
Reference
3  [PMID:12954640]
  Authors
Uemura S, Kihara A, Inokuchi J, Igarashi Y
  Title
Csg1p and newly identified Csh1p function in mannosylinositol phosphorylceramide  synthesis by interacting with Csg2p.
  Journal
J Biol Chem 278:45049-55 (2003)
DOI:10.1074/jbc.M305498200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.370
IUBMB Enzyme Nomenclature: 2.4.1.370
ExPASy - ENZYME nomenclature database: 2.4.1.370
BRENDA, the Enzyme Database: 2.4.1.370

DBGET integrated database retrieval system