KEGG   ENZYME: 2.4.1.371
Entry
EC 2.4.1.371                Enzyme                                 

Name
polymannosyl GlcNAc-diphospho-ditrans,octacis-undecaprenol 2,3-alpha-mannosylpolymerase;
WbdA
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
GDP-alpha-D-mannose:alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol 2,3-alpha-mannosyltransferase (configuration-retaining)
Reaction(IUBMB)
(1) 2 GDP-alpha-D-mannose + [alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol = 2 GDP + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol [RN:R12525];
(2) 2 GDP-alpha-D-mannose + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol = 2 GDP + [alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n+1-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol [RN:R12526]
Reaction(KEGG)
R12525 R12526
Substrate
GDP-alpha-D-mannose [CPD:C00096];
[alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol [CPD:C22229];
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol [CPD:C20761]
Product
GDP [CPD:C00035];
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol [CPD:C20761];
[alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n+1-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
Comment
The enzyme is involved in the biosynthesis of polymannose O-polysaccharide in the outer leaflet of the membrane of Escherichia coli serotype O9a. The enzymes consists of two domains that are responsible for the 1->2 and 1->3 linkages, respectively.
History
EC 2.4.1.371 created 2019
Orthology
K12993  O-antigen biosynthesis alpha-1,2-mannosyltransferase/rhamnosyltransferase
Genes
OTW: 112243118
ECX: EcHS_A2168
ECR: ECIAI1_2099(wbdA)
SBG: SBG_0616(wbdA)
SBZ: A464_689
SBV: N643_03105
KPJ: N559_1772
KVA: Kvar_1570
KPE: KPK_1680
KVD: KR75_26280
EAR: CCG29190
YAL: AT01_3975
YIN: CH53_3311
YRO: CH64_53
SMAC: SMDB11_0876(wbdA)
SPLY: Q5A_007975(kanE)
SFJ: SAMEA4384070_1647(mfpsA)
SGL: SG1119
SOD: Sant_1471
XFT: PD_1546(mtfA) PD_1793(mtfA)
XCV: XCV3719(wbdA1) XCV3723(wbdA2)
XAL: XALC_2705
XPH: XppCFBP6546_16320(XppCFBP6546P_16320) XppCFBP6546_16340(XppCFBP6546P_16340)
LEZ: GLE_4241
LEM: LEN_0960(wbpY)
PAE: PA5449(wbpX)
PAEV: N297_5637
PAEI: N296_5637
PAU: PA14_71930(wbpX)
PAP: PSPA7_6239(wbpX)
PAG: PLES_58441(wbpX)
PAF: PAM18_5571(wbpX)
PNC: NCGM2_6226(wbpX)
PAEB: NCGM1900_6304(wbpX)
PAEP: PA1S_29435
PAEM: U769_29940
PAEL: T223_29900
PAEU: BN889_06056(wbpX)
PAEG: AI22_04550
PAEC: M802_5635
PAEO: M801_5502
PPU: PP_1780
PPT: PPS_1421
PPI: YSA_02208
PPX: T1E_3030
PPUT: L483_06430
PSB: Psyr_0920
PFO: Pfl01_5686(wbpX)
PFE: PSF113_5890(wbpX)
PMAN: OU5_3473
PPUU: PputUW4_01525(wbpX)
PKC: PKB_5492
PSES: PSCI_5259
PSEM: TO66_31520
LLO: LLO_3176
TMC: LMI_3105
MEC: Q7C_2298
OCE: GU3_14745
ENM: EBS_2096
RPI: Rpic_1159
REH: H16_A2901(h16_A2901)
BCEN: DM39_689
BCEO: I35_0631
BAM: Bamb_0758
BCT: GEM_2646
BCED: DM42_954
BDL: AK34_2295
BCON: NL30_11355
BUB: BW23_854
BTEI: WS51_14640
BGU: KS03_1586
BGO: BM43_2089
PLG: NCTC10937_04687(mfpsA_3)
HYF: DTO96_100941(mfpsA)
CABA: SBC2_29050(mshA_20)
AMIM: MIM_c06910
CFU: CFU_1121(mtfA)
CARE: LT85_3689
BBAG: E1O_12550
NIM: W01_08490
CJS: CJS3_1523
CJR: CJE1615
CFZ: CSG_6190
SUN: SUN_0328
NIS: NIS_1395
GUR: Gura_3802
CCX: COCOR_02235(pimB2)
DBR: Deba_0452
MHUA: MCHK_5585
ARA: Arad_9152
RPB: RPB_3839
RPD: RPD_1649
RPT: Rpal_4470
NHA: Nham_2772
MDI: METDI5548
MEX: Mext_4511
MCH: Mchl_4974
MPO: Mpop_5026
MOR: MOC_5963
META: Y590_23000
MAQU: Maq22A_2p42095(rfaG) Maq22A_c26480(rfaG)
PLEO: OHA_1_02672(mfpsA_2) OHA_1_03280(mshA_6)
HDI: HDIA_0950
MALU: KU6B_52250(wcbB)
HNE: HNE_0804
NAR: Saro_0750
SPHM: G432_11025
SPKC: KC8_14160
SECH: B18_08510
SSY: SLG_29900
SPMI: K663_14085
SINB: SIDU_13450
SPHT: K426_06665
GOH: B932_0782
GDI: GDI2399
GDJ: Gdia_0643
GXL: H845_2753
APK: APA386B_698(wbpX)
ASZ: ASN_441(wbpX)
AFR: AFE_2967
PMW: B2K_18240
TTM: Tthe_2187
CFL: Cfla_2369
LET: O77CONTIG1_03440(mshA_4)
PRM: EW15_2187
MPK: VL20_4000
CYT: cce_0516
AVA: Ava_0349
CAG: Cagg_2760
STI: Sthe_1323
OBG: Verru16b_01997(mshA_2)
MIN: Minf_1305(rfaG)
MKC: kam1_1319
PIR: VN12_04585(mshA_2)
BVO: Pan97_07350(mshA_2) Pan97_45480(gtf1)
SACI: Sinac_2256
AGV: OJF2_31370(sunS_2) OJF2_63320(sunS_4)
SUS: Acid_6233
ABAC: LuPra_03616 LuPra_03617(mfpsA)
AAE: aq_1080 aq_1096(mtfA)
AFU: AF_0045
MHOR: MSHOH_3111
MTP: Mthe_1073
MCJ: MCON_1850
MHI: Mhar_1106
 » show all
Reference
1  [PMID:22875852]
  Authors
Greenfield LK, Richards MR, Li J, Wakarchuk WW, Lowary TL, Whitfield C
  Title
Biosynthesis of the polymannose lipopolysaccharide O-antigens from Escherichia coli serotypes O8 and O9a requires a unique combination of single- and multiple-active site mannosyltransferases.
  Journal
J Biol Chem 287:35078-91 (2012)
DOI:10.1074/jbc.M112.401000
  Sequence
Reference
2  [PMID:22989876]
  Authors
Greenfield LK, Richards MR, Vinogradov E, Wakarchuk WW, Lowary TL, Whitfield C
  Title
Domain organization of the polymerizing mannosyltransferases involved in synthesis of the Escherichia coli O8 and O9a lipopolysaccharide O-antigens.
  Journal
J Biol Chem 287:38135-49 (2012)
DOI:10.1074/jbc.M112.412577
  Sequence
Reference
3  [PMID:25422321]
  Authors
Liston SD, Clarke BR, Greenfield LK, Richards MR, Lowary TL, Whitfield C
  Title
Domain interactions control complex formation and polymerase specificity in the biosynthesis of the Escherichia coli O9a antigen.
  Journal
J Biol Chem 290:1075-85 (2015)
DOI:10.1074/jbc.M114.622480
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.371
IUBMB Enzyme Nomenclature: 2.4.1.371
ExPASy - ENZYME nomenclature database: 2.4.1.371
BRENDA, the Enzyme Database: 2.4.1.371

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