KEGG ENZYME: 2.4.1.387

ENZYME: 2.4.1.387

Entry

EC 2.4.1.387                Enzyme

Name

isomaltosyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

alpha-isomaltosyl-(1->3)-1,4-alpha-D-glucan:1,4-alpha-D-glucan 3-alpha-isomaltosyltransferase

Reaction(IUBMB)

(1) 2 alpha-isomaltosyl-(1->4)-maltotriose = alpha-isomaltosyl-(1->3)-alpha-isomaltosyl-(1->4)-maltotriose + maltotriose [RN:R12984];
(2) alpha-isomaltosyl-(1->3)-alpha-isomaltosyl-(1->4)-maltotriose = cyclobis-(1->6)-alpha-nigerosyl + maltotriose [RN:R12985]

Reaction(KEGG)

R12984 R12985

Substrate

alpha-isomaltosyl-(1->4)-maltotriose [CPD:C22497];
alpha-isomaltosyl-(1->3)-alpha-isomaltosyl-(1->4)-maltotriose [CPD:C22498]

Product

alpha-isomaltosyl-(1->3)-alpha-isomaltosyl-(1->4)-maltotriose [CPD:C22498];
maltotriose [CPD:C01835];
cyclobis-(1->6)-alpha-nigerosyl

Comment

The enzyme, found in bacteria that produce cyclobis-(1->6)-alpha-nigerosyl, acts on the products of EC 2.4.1.24, 1,4-alpha-glucan 6-alpha;-glucosyltransferase. It catalyses the alpha-(1->3) transfer of the isomaltosyl moiety of one substrate to another, resulting in alpha-isomaltosyl-(1->3)-alpha-isomaltosyl-alpha-(1->4)-glucan formation. In addition, the enzyme catalyses the intramolecular cyclization of the product, eventually generating cyclobis-(1->6)-alpha-nigerosyl.

History

EC 2.4.1.387 created 2022

Orthology

K25763

isomaltosyltransferase

Genes

FPN:	ABE65_011630

LMO:

lmo2446

LMN:	LM5578_2642

LMY:	LM5923_2591

LMT:	LMRG_01802

LMOC:

LMOSLCC5850_2448

LMOD:

LMON_2457

LMOW:

AX10_06295

LMR:	LMR479A_2571

LMOM:

IJ09_12580

LMF:	LMOf2365_2419

LMC:	Lm4b_02415

LMOG:

BN389_24090

LMP:

MUO_12210

LMOL:

LMOL312_2406

LMOJ:

LM220_21110

LMOZ:

LM1816_14045

LMOX:

AX24_10150

LMH:	LMHCC_0154

LMQ:

LMM7_2488

LML:	lmo4a_2449

LMG:	LMKG_02691

LMS:

LMLG_2125

LMJ:	LMOG_02419

LMW:	LMOSLCC2755_2450

LMX:	LMOSLCC2372_2508

LMZ:	LMOSLCC2482_2449

LMON:

LMOSLCC2376_2338

LMOS:

LMOSLCC7179_2358

LMOO:

LMOSLCC2378_2449

LMOY:

LMOSLCC2479_2508

LMOT:

LMOSLCC2540_2479

LMOA:

LMOATCC19117_2455

LMOK:

CQ02_12415

LMV:	Y193_03490

LIN:

lin2540

LSG:

lse_2345

LIV:

LIV_2351

LII:	JL52_12415

LIW:	AX25_12605

LIA:	JL58_12605

LIO:	JL53_13095

LMAR:

LAX80_011805

COHN:

KCTCHS21_11340

COHL:

J4772_27950

CTHD:

CDO33_10360

FPLA:

A4U99_11350

VPY:	HZI73_00990

PPHP:

VTA80_15110

MHOS:

CXR34_12465

MBIN:

LXM64_10400

MICP:

KY498_00220

KFL:

Kfla_4052

KQI:	F1D05_29315

KRI:	OX958_26750

NOA:	BKM31_21900

NGN:	LCN96_29635

TPYO:

X956_02305

» show all

Reference

1 [PMID:12092816]

Authors

Aga H, Maruta K, Yamamoto T, Kubota M, Fukuda S, Kurimoto M, Tsujisaka Y.

Title

Cloning and sequencing of the genes encoding cyclic tetrasaccharide-synthesizing enzymes from Bacillus globisporus C11.

Journal

Biosci Biotechnol Biochem 66:1057-68 (2002)
DOI:10.1271/bbb.66.1057

Sequence

[ag:BAB88403]

Reference

2 [PMID:12400677]

Authors

Nishimoto T, Aga H, Mukai K, Hashimoto T, Watanabe H, Kubota M, Fukuda S, Kurimoto M, Tsujisaka Y.

Title

Purification and characterization of glucosyltransferase and glucanotransferase involved in the production of cyclic tetrasaccharide in Bacillus globisporus C11.

Journal

Biosci Biotechnol Biochem 66:1806-18 (2002)
DOI:10.1271/bbb.66.1806

Reference

3 [PMID:14553982]

Authors

Kim YK, Kitaoka M, Hayashi K, Kim CH, Cote GL.

Title

A synergistic reaction mechanism of a cycloalternan-forming enzyme and a D-glucosyltransferase for the production of cycloalternan in Bacillus sp. NRRL B-21195.

Journal

Carbohydr Res 338:2213-20 (2003)
DOI:10.1016/s0008-6215(03)00375-6

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.387

IUBMB Enzyme Nomenclature:

2.4.1.387

ExPASy - ENZYME nomenclature database:

2.4.1.387

BRENDA, the Enzyme Database:

2.4.1.387

All links

Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
All databases (2)   

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