KEGG   ENZYME: 2.4.1.70
Entry
EC 2.4.1.70                 Enzyme                                 

Name
poly(ribitol-phosphate) alpha-N-acetylglucosaminyltransferase;
TarM;
UDP acetylglucosamine-poly(ribitol phosphate) acetylglucosaminyltransferase (ambiguous);
uridine diphosphoacetylglucosamine-poly(ribitol phosphate) acetylglucosaminyltransferase (ambiguous);
UDP-N-acetyl-D-glucosamine:poly(ribitol-phosphate) N-acetyl-D-glucosaminyltransferase (ambiguous);
UDP-N-acetyl-alpha-D-glucosamine:poly(ribitol-phosphate) N-acetyl-alpha-D-glucosaminyltransferase (ambiguous);
poly(ribitol-phosphate) N-acetylglucosaminyltransferase (ambiguous)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-N-acetyl-alpha-D-glucosamine:4-O-(D-ribitylphospho)n-di[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol alpha-N-acetyl-D-glucosaminyltransferase (configuration-retaining)
Reaction(IUBMB)
n UDP-N-acetyl-alpha-D-glucosamine + 4-O-(D-ribitylphospho)n-di[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = n UDP + 4-O-(2-N-acetyl-alpha-D-glucosaminyl-D-ribitylphospho)n-di[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol [RN:R12037]
Reaction(KEGG)
R12037;
(other) R02717 R06182(G)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
4-O-(D-ribitylphospho)n-di[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol [CPD:C21508]
Product
UDP [CPD:C00015];
4-O-(2-N-acetyl-alpha-D-glucosaminyl-D-ribitylphospho)n-di[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol [CPD:C21870]
Comment
Involved in the biosynthesis of poly(ribitol phosphate) teichoic acids in the cell wall of the bacterium Staphylococcus aureus. This enzyme adds an N-acetyl-alpha-D-glucosamine to the hydroxyl group at the 2 position of the ribitol phosphate units. cf. EC 2.4.1.355 [poly(ribitol-phosphate) beta-N-acetylglucosaminyltransferase].
History
EC 2.4.1.70 created 1972, modified 2018
Orthology
K22710  poly(ribitol-phosphate) alpha-N-acetylglucosaminyltransferase
Genes
SAM: MW0919
SAS: SAS0971
SAC: SACOL1043
SAX: USA300HOU_0984
SAA: SAUSA300_0939
SAE: NWMN_0906
SUV: SAVC_04345
SUJ: SAA6159_00892
SUK: SAA6008_00990
SUT: SAT0131_01070
SUQ: HMPREF0772_12199
SUZ: MS7_0993
SUW: SATW20_10320
SAUA: SAAG_02146
SAUZ: SAZ172_0976
SAUF: X998_1035
SAB: SAB0901
SAUM: BN843_9420
SAUR: SABB_01002
SAUI: AZ30_04940
SUH: SAMSHR1132_08830
SDP: NCTC12225_00342(tagE_1)
SSCH: LH95_01430
SKL: C7J89_02280
SARL: SAP2_11820
SSH: NCTC13712_00981(tagE_3)
SSIM: SAMEA4384339_0209(tagE_1)
SFF: FOB90_06365
SSTE: SAMEA4384403_0871(tagE_1)
MLEN: H3V22_11340
GPR: JQN66_07680
PAEY: KUF55_10590
 » show all
Reference
1
  Authors
Nathenson, S.G., Ishimoto, N. and Strominger, J.L.
  Title
UDP-N-acetylglucosamine:polyribitol phosphate N-acetylglucosaminyltransferases from Staphylococcus aureus.
  Journal
Methods Enzymol 8:426-429 (1966)
Reference
2  [PMID:20185825]
  Authors
Xia G, Maier L, Sanchez-Carballo P, Li M, Otto M, Holst O, Peschel A
  Title
Glycosylation of wall teichoic acid in Staphylococcus aureus by TarM.
  Journal
J Biol Chem 285:13405-15 (2010)
DOI:10.1074/jbc.M109.096172
  Sequence
[sac:SACOL1043]
Reference
3  [PMID:25624472]
  Authors
Sobhanifar S, Worrall LJ, Gruninger RJ, Wasney GA, Blaukopf M, Baumann L, Lameignere E, Solomonson M, Brown ED, Withers SG, Strynadka NC
  Title
Structure and mechanism of Staphylococcus aureus TarM, the wall teichoic acid alpha-glycosyltransferase.
  Journal
Proc Natl Acad Sci U S A 112:E576-85 (2015)
DOI:10.1073/pnas.1418084112
  Sequence
[sac:SACOL1043]
Reference
4  [PMID:25697358]
  Authors
Koc C, Gerlach D, Beck S, Peschel A, Xia G, Stehle T
  Title
Structural and enzymatic analysis of TarM glycosyltransferase from Staphylococcus aureus reveals an oligomeric protein specific for the glycosylation of wall teichoic acid.
  Journal
J Biol Chem 290:9874-85 (2015)
DOI:10.1074/jbc.M114.619924
  Sequence
[sac:SACOL1043]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.70
IUBMB Enzyme Nomenclature: 2.4.1.70
ExPASy - ENZYME nomenclature database: 2.4.1.70
BRENDA, the Enzyme Database: 2.4.1.70
CAS: 37277-71-7

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Chemical substance (10)   
   KEGG COMPOUND (6)   
   KEGG GLYCAN (4)   
Chemical reaction (5)   
   KEGG REACTION (3)   
   KEGG RCLASS (2)   
Gene (33)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (32)   
Protein sequence (3)   
   UniProt (2)   
   SWISS-PROT (1)   
DNA sequence (1)   
   GenBank (1)   
Protein domain (1)   
   InterPro (1)   
All databases (53)   

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