Entry
Name
NAD+---dinitrogen-reductase ADP-D-ribosyltransferase;
NAD-azoferredoxin (ADPribose)transferase;
NAD-dinitrogen-reductase ADP-D-ribosyltransferase;
draT (gene name)
Class
Transferases;
Glycosyltransferases;
Pentosyltransferases
BRITE hierarchy
Sysname
NAD+:[dinitrogen reductase] (ADP-D-ribosyl)transferase
Reaction(IUBMB)
NAD+ + [dinitrogen reductase]-L-arginine = nicotinamide + [dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine [RN:
R03447 ]
Reaction(KEGG)
Substrate
NAD+ [CPD:
C00003 ];
[dinitrogen reductase]-L-arginine [CPD:
C03315 ]
Product
nicotinamide [CPD:
C00153 ];
[dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine [CPD:
C01253 ]
Comment
The combined action of this enzyme and EC
3.2.2.24 , ADP-ribosyl-[dinitrogen reductase] hydrolase, controls the activity level of nitrogenase (EC
1.18.6.1 ). In the presence of ammonium, the product of nitrogenase, this enzyme covalently links an ADP-ribose moiety to a specific arginine residue of the dinitrogenase reductase component of nitrogenase, blocking its activity.
History
EC 2.4.2.37 created 1992, modified 2015
Orthology
K05951 NAD+---dinitrogen-reductase ADP-D-ribosyltransferase
Genes
TFRI : Thiofri_01194(draT)
RPA : TX73_007350 TX73_012435
RPX : Rpdx1_3108 Rpdx1_3129
RPM : RSPPHO_00485 RSPPHO_02585(draT)
GEV : GSVR_33720 GSVR_40400(draT)
GBN : GEOBRER4_11380(draT)
» show all
Taxonomy
Reference
Authors
Lowery RG, Ludden PW.
Title
Purification and properties of dinitrogenase reductase ADP-ribosyltransferase from the photosynthetic bacterium Rhodospirillum rubrum.
Journal
J Biol Chem 263:16714-9 (1988)
Reference
Authors
Fitzmaurice WP, Saari LL, Lowery RG, Ludden PW, Roberts GP
Title
Genes coding for the reversible ADP-ribosylation system of dinitrogenase reductase from Rhodospirillum rubrum.
Journal
Sequence
Reference
Authors
Moure VR, Costa FF, Cruz LM, Pedrosa FO, Souza EM, Li XD, Winkler F, Huergo LF
Title
Regulation of nitrogenase by reversible mono-ADP-ribosylation.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 2.4.2.37
ExPASy - ENZYME nomenclature database: 2.4.2.37