Entry
Name
lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase;
undecaprenyl phosphate-alpha-L-Ara4N transferase;
4-amino-4-deoxy-L-arabinose lipid A transferase;
polymyxin resistance protein PmrK;
arnT (gene name)
Class
Transferases;
Glycosyltransferases;
Pentosyltransferases
BRITE hierarchy
Sysname
4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl-phosphate:lipid IVA 4-amino-4-deoxy-L-arabinopyranosyltransferase
Reaction(IUBMB)
(1) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A = alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A + ditrans,octacis-undecaprenyl phosphate [RN:
R09781 ];
(2) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + lipid IVA = lipid IIA + ditrans,octacis-undecaprenyl phosphate [RN:
R09773 ];
(3) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA = 4'-alpha-L-Ara4N-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + ditrans,octacis-undecaprenyl phosphate [RN:
R09774 ]
Reaction(KEGG)
Substrate
4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate [CPD:
C16157 ];
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A [CPD:
C06026 ];
lipid IVA [CPD:
C04919 ];
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA
Product
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A [CPD:
C19890 ];
ditrans,octacis-undecaprenyl phosphate [CPD:
C17556 ];
lipid IIA [CPD:
C19884 ];
4'-alpha-L-Ara4N-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA
Comment
Integral membrane protein present in the inner membrane of certain Gram negative endobacteria. In strains that do not produce 3-deoxy-D-manno-octulosonic acid (Kdo), the enzyme adds a single arabinose unit to the 1-phosphate moiety of the tetra-acylated lipid A precursor, lipid IVA. In the presence of a Kdo disaccharide, the enzyme primarily adds an arabinose unit to the 4-phosphate of lipid A molecules. The Salmonella typhimurium enzyme can add arabinose units to both positions.
History
EC 2.4.2.43 created 2010, modified 2011
Pathway
ec00540 Lipopolysaccharide biosynthesis
Orthology
K07264 4-amino-4-deoxy-L-arabinose transferase
Genes
ECOO : ECRM13514_3013(arnT)
ECOH : ECRM13516_2958(arnT)
ELR : ECO55CA74_13845(arnT)
ECW : EcE24377A_2552(arnT)
EDJ : ECDH1ME8569_2193(arnT)
ECOI : ECOPMV1_02418(arnT)
SEM : STMDT12_C23220(arnT)
SETC : CFSAN001921_05290(arnT)
SEEN : SE451236_17700(arnT)
SENH : CFSAN002069_20340(arnT)
SEEH : SEEH1578_20780(arnT)
SENJ : CFSAN001992_22070(arnT)
SEEC : CFSAN002050_18430(arnT)
SEEB : SEEB0189_008065(arnT)
SENC : SEET0819_18595(arnT)
REE : electrica_00261(arnT)
RTG : NCTC13098_00335(arnT)
CLAP : NCTC11466_02464(arnT)
KIE : NCTC12125_04157(arnT)
AHN : NCTC12129_01390(arnT)
METY : MRY16398_42530(arnT)
MPHG : MPHASIOC01_000346(arnT)
YPG : YpAngola_A2608(arnT)
YPI : YpsIP31758_1729(arnT)
SMW : SMWW4_v1c21870(arnT)
SERS : SERRSCBI_10255(arnT)
SFJ : SAMEA4384070_2216(arnT)
SOF : NCTC11214_04676(arnT_1) NCTC11214_04677(arnT_2)
SSAR : SSARUM_002081(arnT)
DIC : Dpoa569_0003915(arnT)
SOD : Sant_1034(arnT1) Sant_3567(arnT2)
PES : SOPEG_0622(arnT1) SOPEG_3211(arnT2)
PLF : PANA5342_p10124(arnT)
PVA : Pvag_pPag10068(yfbI)
PANT : PSNIH1_p00705(arnT)
PDIS : D8B20_18370 D8B20_20965
PPHO : CTZ24_21715 CTZ24_22680
TPTY : NCTC11468_02695(arnT)
PMR : PMI0275(arnT) PMI1047(arnT)
PMIB : BB2000_0425(arnT) BB2000_1084(arnT)
PVG : CRN77_13910 CRN77_18025
PHAU : PH4a_11270 PH4a_14265
PROT : BTA34_03065 BTA34_06485
PCOL : F1325_04285(arnT) F1325_08410(arnT)
PCIB : F9282_04080(arnT) F9282_08360(arnT)
PAPP : QQS39_04545(arnT) QQS39_07790(arnT)
PSI : S70_08185(arnT) S70_19375(arnT)
PSX : DR96_1542(arnT) DR96_2787
PSTA : BGK56_01440 BGK56_11225
PTHA : OI982_03960(arnT) OI982_14955(arnT)
PRG : RB151_004810(arnT_1) RB151_019270(arnT_3)
PALA : CO695_02470 CO695_15990
PHEI : NCTC12003_00479(arnT_1) NCTC12003_02330(arnT_2)
PRQ : CYG50_07540(arnT) CYG50_13705(arnT)
PRJ : NCTC6933_00543(arnT_1) NCTC6933_01645(arnT_3)
PVC : G3341_02195(arnT) G3341_11925(arnT)
PMAG : JI723_02490(arnT) JI723_11830(arnT)
PHAG : PZ638_02225(arnT) PZ638_12435(arnT)
PZJ : QS795_001970(arnT) QS795_006965(arnT)
ANS : ArsFIN_16520(arnT_1)
MWI : MNY66_06905(arnT) MNY66_13895(arnT)
PAEB : NCGM1900_2727(arnT)
PCQ : PcP3B5_10950(arnT_1)
PPSL : BJP27_03465 BJP27_15095
PFUV : NLK61_00300(arnT) NLK61_28135(arnT)
PFL : PFL_3047(arnT_1) PFL_4461(arnT_2)
PPRC : PFLCHA0_c30750(arnT1) PFLCHA0_c45320(arnT2)
PPRO : PPC_3075(arnT_1) PPC_4570(arnT_2)
PFN : HZ99_03630 HZ99_09580
PFO : Pfl01_2835 Pfl01_2845
PFC : PflA506_2686(arnT_1) PflA506_3380(arnT_2)
PPZ : H045_08700(arnT) H045_10940(arnT)
PTV : AA957_24955 AA957_29320
PVR : PverR02_15970 PverR02_20470
PAZO : AYR47_22465 AYR47_26715
POI : BOP93_12515 BOP93_16445
PRX : HRH33_14010(arnT) HRH33_17655(arnT)
PMUD : NCTC8068_02556(arnT)
PTOL : I7845_10195(arnT) I7845_14160(arnT)
PMAB : U0037_15210(arnT) U0037_20010(arnT)
PFW : PF1751_v1c26910(arnT) PF1751_v1c34690(arnT)
PFF : PFLUOLIPICF718560 PFLUOLIPICF722860
PFX : A7318_13365 A7318_17090
PSK : U771_15890 U771_21245
PKC : PKB_2816(arnT2) PKB_4723(arnT2)
PCZ : PCL1606_30680 PCL1606_30740
PCP : JM49_15585 JM49_15610
PSEM : TO66_15060 TO66_15085
PPSY : AOC04_08555 AOC04_08695
PSOS : POS17_3051(arnT_1) POS17_4533(arnT_2)
PANR : A7J50_2966 A7J50_3666
PYM : AK972_2046 AK972_2828
PKE : DLD99_14925 DLD99_14975
PZE : HU754_015330(arnT) HU754_015380(arnT)
PMOE : HV782_015435(arnT) HV782_015485(arnT)
PSEP : C4K39_3413 C4K39_5053
PPII : QL104_16060(arnT) QL104_23125(arnT)
PVW : HU752_013160(arnT) HU752_017370(arnT)
PTRT : HU722_0015220(arnT) HU722_0018720(arnT)
PSAM : HU731_001855(arnT) HU731_005340(arnT)
PASG : KSS96_15140(arnT) KSS96_18805(arnT)
PTAE : NCTC10697_02214(arnT_2)
PIZ : LAB08_R27590(arnT) LAB08_R27680(arnT)
PPEG : KUA23_15335(arnT) KUA23_19805(arnT)
PNB : NK667_10525(arnT) NK667_10575(arnT)
PTRL : OU419_05165(arnT) OU419_15145(arnT)
PPAE : LDL65_00690(arnT) LDL65_25705(arnT)
PSHS : JJN09_05175(arnT) JJN09_05225(arnT)
PLIS : NN484_21915(arnT) NN484_21965(arnT)
PSNC : C0058_12085 C0058_16085
PKV : QYQ93_14540(arnT) QYQ93_18675(arnT)
PBAM : NUH87_03245(arnT) NUH87_05600(arnT)
PQE : OSC50_08475(arnT) OSC50_11825(arnT)
PSDT : NH234_14780(arnT) NH234_14830(arnT)
PCAA : V6L78_14585(arnT) V6L78_18785(arnT)
PSML : V6P94_14945(arnT) V6P94_15095(arnT)
PSMZ : V6L79_14975(arnT) V6L79_15115(arnT)
PSIV : LZ023_17945(arnT) LZ023_24885(arnT)
VBC : C5Q97_17320 C5Q97_17325
LBV : JYG24_04285 JYG24_04290 JYG24_10465
» show all
Taxonomy
Reference
Authors
Trent MS, Ribeiro AA, Lin S, Cotter RJ, Raetz CR
Title
An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor.
Journal
Sequence
Reference
Authors
Trent MS, Ribeiro AA, Doerrler WT, Lin S, Cotter RJ, Raetz CR
Title
Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm.
Journal
Reference
Authors
Zhou Z, Ribeiro AA, Lin S, Cotter RJ, Miller SI, Raetz CR
Title
Lipid A modifications in polymyxin-resistant Salmonella typhimurium: PMRA-dependent 4-amino-4-deoxy-L-arabinose, and phosphoethanolamine incorporation.
Journal
Reference
Authors
Bretscher LE, Morrell MT, Funk AL, Klug CS
Title
Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium.
Journal
Sequence
Reference
Authors
Impellitteri NA, Merten JA, Bretscher LE, Klug CS
Title
Identification of a functionally important loop in Salmonella typhimurium ArnT.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 2.4.2.43
ExPASy - ENZYME nomenclature database: 2.4.2.43