| Entry |
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| Name |
lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase;
undecaprenyl phosphate-alpha-L-Ara4N transferase;
4-amino-4-deoxy-L-arabinose lipid A transferase;
polymyxin resistance protein PmrK;
arnT (gene name)
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| Class |
Transferases;
Glycosyltransferases;
Pentosyltransferases
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| Sysname |
4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate:lipid IVA 4-amino-4-deoxy-L-arabinopyranosyltransferase
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| Reaction(IUBMB) |
(1) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A = alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A + ditrans,octacis-undecaprenyl phosphate [RN: R09781];
(2) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + lipid IVA = lipid IIA + ditrans,octacis-undecaprenyl phosphate [RN: R09773];
(3) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA = 4'-alpha-L-Ara4N-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + ditrans,octacis-undecaprenyl phosphate [RN: R09774]
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| Reaction(KEGG) |
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| Substrate |
4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate [CPD: C16157];
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A [CPD: C06026];
lipid IVA [CPD: C04919];
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA
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| Product |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A [CPD: C19890];
ditrans,octacis-undecaprenyl phosphate [CPD: C17556];
lipid IIA [CPD: C19884];
4'-alpha-L-Ara4N-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA
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| Comment |
Integral membrane protein present in the inner membrane of certain Gram negative endobacteria. In strains that do not produce 3-deoxy-D-manno-octulosonic acid (Kdo), the enzyme adds a single arabinose unit to the 1-phosphate moiety of the tetra-acylated lipid A precursor, lipid IVA. In the presence of a Kdo disaccharide, the enzyme primarily adds an arabinose unit to the 4-phosphate of lipid A molecules. The Salmonella typhimurium enzyme can add arabinose units to both positions.
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| History |
EC 2.4.2.43 created 2010, modified 2011
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| Pathway |
| ec00540 | Lipopolysaccharide biosynthesis |
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| Orthology |
| K07264 | 4-amino-4-deoxy-L-arabinose transferase |
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| Genes |
» show all
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| Reference |
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| Authors |
Trent MS, Ribeiro AA, Lin S, Cotter RJ, Raetz CR |
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| Title |
An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Trent MS, Ribeiro AA, Doerrler WT, Lin S, Cotter RJ, Raetz CR |
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| Title |
Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm. |
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| Journal |
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| Reference |
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| Authors |
Zhou Z, Ribeiro AA, Lin S, Cotter RJ, Miller SI, Raetz CR |
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| Title |
Lipid A modifications in polymyxin-resistant Salmonella typhimurium: PMRA-dependent 4-amino-4-deoxy-L-arabinose, and phosphoethanolamine incorporation. |
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| Journal |
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| Reference |
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| Authors |
Bretscher LE, Morrell MT, Funk AL, Klug CS |
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| Title |
Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Impellitteri NA, Merten JA, Bretscher LE, Klug CS |
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| Title |
Identification of a functionally important loop in Salmonella typhimurium ArnT. |
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| Journal |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 2.4.2.43 |
| ExPASy - ENZYME nomenclature database: | 2.4.2.43 |
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