KEGG   ENZYME: 2.4.2.60Help
Entry
EC 2.4.2.60                 Enzyme                                 

Name
cysteine-dependent adenosine diphosphate thiazole synthase;
THI4 (gene name) (ambiguous);
THI1 (gene name);
ADP-thiazole synthase
Class
Transferases;
Glycosyltransferases;
Pentosyltransferases
BRITE hierarchy
Sysname
NAD+:glycine ADP-D-ribosyltransferase (dehydroalanine-producing)
Reaction(IUBMB)
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O [RN:R10685]
Reaction(KEGG)
Substrate
NAD+ [CPD:C00003];
glycine [CPD:C00037];
[ADP-thiazole synthase]-L-cysteine
Product
nicotinamide [CPD:C00153];
ADP-5-ethyl-4-methylthiazole-2-carboxylate [CPD:C20784];
[ADP-thiazole synthase]-dehydroalanine;
H2O [CPD:C00001]
Comment
This iron dependent enzyme, found in fungi and plants, is involved in the thiamine phosphate biosynthesis pathway. It is a single turn-over enzyme since the cysteine residue is not regenerated in vivo [3]. The homologous enzyme in archaea (EC 2.4.2.59, sulfide-dependent adenosine diphosphate thiazole synthase) uses sulfide as sulfur donor.
History
EC 2.4.2.60 created 2018
Pathway
ec00730  Thiamine metabolism
ec01100  Metabolic pathways
Orthology
K03146  cysteine-dependent adenosine diphosphate thiazole synthase
Genes
RSS: 109442182
ATH: AT5G54770(THI1)
ALY: ARALYDRAFT_918614
CRB: 17876896
CSAT: 104726000 104731059 104763460
EUS: EUTSA_v10013978mg
BRP: 103844930 103851941 103857026
BNA: 106346007 106347311 106349644 106366413 106371241 106390546 106402237 106420919 106420920 106439989
BOE: 106318832 106327201 106335239
THJ: 104817652
CPAP: 110810965
EGR: 104450213
LJA: Lj3g3v1010900.1(Lj3g3v1010900.1) Lj5g3v2060670.1(Lj5g3v2060670.1)
PPER: 18780719
PMUM: 103342510
PAVI: 110747499
ZJU: 107420469
CSV: 101211553
CMO: 103501605
MCHA: 111022099
RCU: 8280736
JCU: 105632894
HBR: 110647144
VVI: 100260728(THI1-1) 100265774(THI1-2)
SLY: 101257192
SPEN: 107025837
SOT: 102598996
CANN: 107856341
NTA: 107797059(ThiI) 107798781
NSY: 104224143
NTO: 104087540
NAU: 109232136
SOE: 110795721
NNU: 104604883
OSA: 4343443
DOSA: Os07t0529600-01(Os07g0529600)
OBR: 102709384
BDI: 100841709
ATS: 109771029(LOC109771029)
ZMA: 542714(thi1) 542715(thi2)
DCT: 110108175
PEQ: 110031149
ATR: 18435056
CRE: CHLREDRAFT_196899(THI4b)
APRO: F751_1708
SCE: YGR144W(THI4)
ERC: Ecym_6012
KMX: KLMA_40625(THI4)
NCS: NCAS_0D01750(NCAS0D01750)
NDI: NDAI_0C02860(NDAI0C02860)
TPF: TPHA_0A04790(TPHA0A04790)
TBL: TBLA_0B09390(TBLA0B09390) TBLA_0H00760(TBLA0H00760)
TDL: TDEL_0H00200(TDEL0H00200)
CAL: CAALFM_C305130CA(THI4)
SLB: AWJ20_4223(THI4)
NCR: NCU06110
NTE: NEUTE1DRAFT95662(NEUTE1DRAFT_95662)
MGR: MGG_03098
SSCK: SPSK_07738
TRE: TRIREDRAFT_68608(thi4)
MAW: MAC_01186
MAJ: MAA_05549
CMT: CCM_01639
BFU: BCIN_07g06010(Bcthi4)
MBE: MBM_01288
ANI: AN3928.2
ANG: ANI_1_228094(An11g01630)
ABE: ARB_07940
TVE: TRV_04098
PTE: PTT_08482
SPO: SPBC26H8.01(thi2)
ABP: AGABI1DRAFT40074(AGABI1DRAFT_40074)
ABV: AGABI2DRAFT72787(AGABI2DRAFT_72787)
WSE: WALSEDRAFT_32181(CYPBP37)
MGL: MGL_1314
SMIN: v1.2.003820.t1(symbB.v1.2.003820.t1)
SPAR: SPRG_04526
 » show all
Taxonomy
Reference
1  [PMID:16912043]
  Authors
Godoi PH, Galhardo RS, Luche DD, Van Sluys MA, Menck CF, Oliva G
  Title
Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana.
  Journal
J Biol Chem 281:30957-66 (2006)
DOI:10.1074/jbc.M604469200
  Sequence
[ath:AT5G54770]
Reference
2  [PMID:22031445]
  Authors
Chatterjee A, Abeydeera ND, Bale S, Pai PJ, Dorrestein PC, Russell DH, Ealick SE, Begley TP.
  Title
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase.
  Journal
Nature 478:542-6 (2011)
DOI:10.1038/nature10503
  Sequence
[sce:YGR144W]
Reference
3  [PMID:26919468]
  Authors
Zhang X, Eser BE, Chanani PK, Begley TP, Ealick SE
  Title
Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole  Synthases.
  Journal
Biochemistry 55:1826-38 (2016)
DOI:10.1021/acs.biochem.6b00030
  Sequence
[sce:YGR144W]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.2.60
IUBMB Enzyme Nomenclature: 2.4.2.60
ExPASy - ENZYME nomenclature database: 2.4.2.60
BRENDA, the Enzyme Database: 2.4.2.60

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