KEGG ENZYME: 2.4.99.21

ENZYME: 2.4.99.21

Entry

EC 2.4.99.21                Enzyme

Name

dolichyl-phosphooligosaccharide-protein glycotransferase;
AglB;
archaeal oligosaccharyl transferase;
dolichyl-monophosphooligosaccharide-protein glycotransferase

Class

Transferases;
Glycosyltransferases;
Transferring other glycosyl groups

Sysname

dolichyl-phosphooligosaccharide:protein-L-asparagine N-beta-D-oligosaccharidotransferase

Reaction(IUBMB)

an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine

Substrate

archaeal dolichyl phosphooligosaccharide;
[protein]-L-asparagine

Product

archaeal dolichyl phosphate [CPD:C21212];
glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine

Comment

The enzyme, characterized from the archaea Methanococcus voltae and Haloferax volcanii, transfers a glycan component from dolichyl phosphooligosaccharide to external proteins. It is different from EC 2.4.99.18, dolichyl-diphosphooligosaccharide-protein glycotransferase, which uses dolichyl diphosphate as carrier compound in bacteria and eukaryotes. The enzyme participates in the N-glycosylation of proteins in some archaea. It requires Mn2+. Dolichol used by archaea is different from that used by eukaryotes. It is much shorter (C55-C60), it is alpha,omega-saturated and it may have additional unsaturated positions in the chain.

History

EC 2.4.99.21 created 2015

Orthology

K21306

dolichyl-phosphooligosaccharide-protein glycotransferase

Genes

GEO:	Geob_1424 Geob_2990

DLI:

dnl_42750

SUR:	STAUR_5887

AGE:	AA314_03686

AVM:	JQX13_32570

CFUS:

CYFUS_005592

MTAU:

JS510_00660

VAI:	BU251_01235

CTM:	Cabther_A0306

CVL:	J8C06_02030

CHLO:

J8C02_01495

MJA:

MJ_1525

MFE:	Mefer_0590

MVU:	Metvu_0243

MFS:	MFS40622_0538

MIF:	Metin_1222

MJH:	JH146_0370

MESG:

MLAUSG7_0298(aglB)

MESA:

MLASG1_1379(aglB)

MIG:	Metig_1797

MMP:

MMP1424

MMQ:	MmarC5_0154

MMX:	MmarC6_1249

MMZ:	MmarC7_0669

MMD:

GYY_07955

MMAK:

MMKA1_16480

MMAO:

MMOS7_15310

MMAD:

MMJJ_14150

MAE:

Maeo_1409

MVN:	Mevan_0735

MVO:

Mvol_1038

MOK:	Metok_0791

METF:

CFE53_03490

MTH:	MTH_1623 MTH_1898 MTH_1906

MMG:	MTBMA_c02090 MTBMA_c04670

MWO:	MWSIV6_0169(stt3) MWSIV6_0430 MWSIV6_0432(aglB) MWSIV6_0805

MTEE:

MTTB_11640 MTTB_13750

METC:

MTCT_1482 MTCT_1729 MTCT_1730

METE:

tca_01572(pglB_2) tca_01834 tca_01844(pglB_3)

METZ:

METMT2_0226 METMT2_1463

METK:

FVF72_00310 FVF72_02210 FVF72_02215 FVF72_03505

MTHM:

FZP57_00735 FZP57_02025 FZP57_02030 FZP57_03850

METJ:

FZP68_05750 FZP68_07045 FZP68_07050

MST:

Msp_0368

METB:

AW729_05230

MRU:

mru_0391

MSI:

Msm_0716

MEB:

Abm4_0186

MMIL:

sm9_2154

MEYE:

TL18_01100

MOL:

YLM1_0063

MARO:

MarbSA_10000

METV:

K4897_04125

MEL:	Metbo_0534 Metbo_0719

MEW:	MSWAN_1515 MSWAN_1516 MSWAN_1855

METH:

MBMB1_1244 MBMB1_1488

MFC:	BRM9_0454 BRM9_0715

MFI:	DSM1535_0382 DSM1535_0667

MCUB:

MCBB_1451 MCBB_1452(aglB_1) MCBB_1453(aglB_3) MCBB_1727

MSUB:

BK009_06390

METN:

BK008_00625 BK008_01980

METT:

CIT01_06795 CIT01_08560 CIT01_08565

METO:

CIT02_10590 CIT02_11860

MEME:

HYG87_07520 HYG87_09010

MFV:	Mfer_0177 Mfer_0275 Mfer_0623

AFU:	AF_0040 AF_0380

AFG:	AFULGI_00003280 AFULGI_00003800

APO:	Arcpr_0726 Arcpr_1194

AVE:	Arcve_0568

AST:	Asulf_01983

FPL:

Ferp_2437

GAC:

GACE_1401

GAH:

GAH_01455

PFU:	PF0156 PF0411

PFI:	PFC_01140 PFC_07420

PHO:	PH0242(PH0242) PH1271(PH1271)

PAB:	PAB1586 PAB2202

PYN:	PNA2_0761 PNA2_1113

PYA:	PYCH_17920 PYCH_19200

PYS:	Py04_0309 Py04_0456

PYC:	TQ32_00625 TQ32_01355

TKO:	TK0810 TK1718

TON:	TON_0775 TON_1820

TGA:	TGAM_0406 TGAM_0937

TSI:	TSIB_0007 TSIB_0418

TBA:	TERMP_00665 TERMP_02078 TERMP_02121

THE:	GQS_01010 GQS_05995 GQS_06090

THA:	TAM4_1026 TAM4_672

THM:	CL1_0839 CL1_0859 CL1_1904

TLT:	OCC_09883 OCC_13840 OCC_13845

THS:	TES1_0603 TES1_2025

TNU:	BD01_0960 BD01_1483

TEU:	TEU_03805 TEU_10605

TGY:	X802_05945 X802_10390

THV:	ADU37_CDS03470 ADU37_CDS21770

TCH:	CHITON_0376 CHITON_0533

TPEP:

A0127_01040 A0127_06605

TPIE:

A7C91_10070

TGG:	A3K92_05705

TCE:	A3L02_01695 A3L02_08450

TBS:	A3L01_04670 A3L01_10285

THH:	CDI07_00005 CDI07_04540

TSL:	A3L11_03485 A3L11_03600 A3L11_08810

TTD:	A3L14_01205 A3L14_06640

TPRF:

A3L09_06860 A3L09_10205 A3L09_10300

TRL:	A3L10_01955 A3L10_09685

TPAF:

A3L08_07280

THY:	A3L12_02835 A3L12_07965

TIC:	FH039_01740 FH039_08440

TCQ:	TIRI35C_0153 TIRI35C_0174 TIRI35C_1192

THEM:

FPV09_01110 FPV09_07665

THEI:

K1720_05200

PPAC:

PAP_06870 PAP_09855

MBA:	Mbar_A0242 Mbar_A0243 Mbar_A0368

MBY:	MSBRM_0550 MSBRM_2711 MSBRM_2714 MSBRM_2715

MBW:	MSBRW_0630 MSBRW_0752 MSBRW_0753

MBAR:

MSBR2_1828 MSBR2_2667 MSBR2_2668 MSBR2_2669

MBAK:

MSBR3_2756 MSBR3_2757 MSBR3_2758 MSBR3_2759 MSBR3_2884

MAC:	MA_1172 MA_3752 MA_3753

MMA:	MM_0647 MM_2210

MMAZ:

MmTuc01_0661 MmTuc01_2261

MMJ:	MSMAS_0662 MSMAS_0663 MSMAS_2483

MMAC:

MSMAC_0664 MSMAC_0665 MSMAC_1961

MVC:	MSVAZ_0691 MSVAZ_0693 MSVAZ_3027

MEK:	MSKOL_0669 MSKOL_0670 MSKOL_3009

MLS:	MSLAZ_0721 MSLAZ_2435

METM:

MSMTP_0730 MSMTP_0868

MEF:	MSWH1_0825 MSWH1_2215

MEQ:	MSWHS_0883 MSWHS_2866

MSJ:	MSSAC_1266 MSSAC_3560 MSSAC_3561 MSSAC_3562

MSZ:	MSSIH_2848 MSSIH_3102 MSSIH_3103 MSSIH_3104

MSW:	MSSIT_2883 MSSIT_3159 MSSIT_3160 MSSIT_3161

MTHR:

MSTHT_2372 MSTHT_2373 MSTHT_2469

MTHE:

MSTHC_0813 MSTHC_0907 MSTHC_0908

MHOR:

MSHOH_0783 MSHOH_0784 MSHOH_0785 MSHOH_3118

MFZ:	AOB57_003075 AOB57_003555 AOB57_003560

MBU:

Mbur_1579

MMET:

MCMEM_2091

MELO:

J7W08_09420

MMH:

Mmah_0123

MHAZ:

BHR79_02840

MEV:	Metev_1257

MZH:	Mzhil_1653

MPY:

Mpsy_2355

MZI:	HWN40_06640 HWN40_10570

MHZ:	Metho_0214 Metho_0482

MTP:	Mthe_1164 Mthe_1498

MCJ:	MCON_1133 MCON_1444

MHI:	Mhar_0540 Mhar_1091 Mhar_1439 Mhar_1730

MHU:	Mhun_2859 Mhun_3138

MRTJ:

KHC33_01570 KHC33_02655 KHC33_16045

MLA:

Mlab_0662

MEM:	Memar_0175 Memar_2235

MBG:	BN140_1425

MEMA:

MMAB1_1829

MCHK:

MchiMG62_25530 MchiMG62_25540

MSUM:

OH143_05460 OH143_07655

MPI:	Mpet_0084 Mpet_2443

MBN:	Mboo_0249 Mboo_1209

MFO:	Metfor_0235 Metfor_0449 Metfor_2864

MPL:

Mpal_0785

MPD:	MCP_2705 MCP_2723

MEZ:	Mtc_0182 Mtc_0183 Mtc_0205

RCI:	LRC539 LRC558

HAL:	VNG_1068G(tot)

HSL:	OE_2548F(aglB)

HDL:	HALDL1_10975

HHB:	Hhub_1167 Hhub_2271(aglB)

HAGS:

JT689_05635

HABO:

JRZ79_04100

HNO:	LT974_07075

HLU:	LT972_06890

HANR:

LJ422_04380

HJE:	HacjB3_10630

HALH:

HTSR_0845(aglB)

HHSR:

HSR6_0872(aglB)

HSU:	HLASF_1520(aglB)

HSF:	HLASA_1507(aglB)

SALR:

FQU85_09050

HALR:

EFA46_009405

HAAD:

MW046_02035 MW046_17750

HALX:

M0R89_17325

HAXZ:

M0R88_17615

SALI:

L593_04990

SAIM:

K0C01_09230

HARA:

AArcS_1839(aglS2)

HMA:	rrnAC0431(tot)

HHI:	HAH_1202(stt3)

HHN:	HISP_06150

HAB:	SG26_13800

HTA:	BVU17_05845

HALJ:

G9465_10525

HSIN:

KDQ40_07485

NPH:	NP_3720A(aglB)

NMO:	Nmlp_2363(aglB)

NHO:	HWV23_01680

NSAL:

HWV07_01780

HUT:	Huta_2131 Huta_2808

HTI:	HTIA_0988 HTIA_2714

HALA:

Hrd1104_03925

HMU:

Hmuk_2752

HALZ:

E5139_01055

HALL:

LC1Hm_2096(aglB)

HAZP:

GBQ70_01055

HALI:

BV210_00425

HSN:	DV733_10970 DV733_14250 DV733_16010

HDS:	HSR122_1829(aglS)

HRR:	HZS55_05895 HZS55_17075

HPEL:

HZS54_11250 HZS54_20445

HLT:	I7X12_18565

HARC:

HARCEL1_10660

HDO:	MUK72_10570

SSAI:

N0B31_08725

HWA:	HQ_2681A(aglB)

HWC:	Hqrw_3013(aglB) Hqrw_3360

HVO:	HVO_1530(aglB)

HME:	HFX_1592(stt3)

HGI:	ABY42_07445

HALE:

G3A49_10770

HLS:	KU306_12730

HLN:	SVXHx_1476(aglb) SVXHx_3241(aglb)

HBO:	Hbor_17000

HAQ:	DU484_07450 DU484_15375

HAJ:	DU500_15395

HAER:

DU502_05710 DU502_13475

HRA:	EI982_08080 EI982_08355 EI982_17700

HLM:	DV707_04230

HALM:

FCF25_14555

HLA:

Hlac_1062

HALP:

DOS48_05360

HALB:

EKH57_05760

HEZZ:

EO776_06735

HALQ:

Hrr1229_001115 Hrr1229_001220

HSS:	J7656_09935

HSAI:

HPS36_00285

HAZZ:

KI388_04265

SRUB:

C2R22_09340 C2R22_20965

HAE:	halTADL_2411

HALN:

B4589_014265

HALG:

HUG10_00440

HALU:

HUG12_17320

HMP:	K6T50_14730

HRE:	K6T36_14685

HRM:	K6T25_02065

HACB:

Hbl1158_06790

HDF:	AArcSl_2614(aglB2)

HAH:	Halar_1620

HTU:

Htur_2957

HSAL:

JMJ58_13645

HAKZ:

J0X25_01415

NMG:	Nmag_0927(aglB)

HXA:	Halxa_2340

NAT:

NJ7G_1722

NPE:	Natpe_1007

NVR:	FEJ81_05205

NPL:	FGF80_05650

NAY:	HYG81_05440

HALY:

HYG82_08425

HJT:	DVR14_14335 DVR14_18215 DVR14_22345

HLO:	J0X27_11635

NGE:	Natgr_2245

HRU:	Halru_0989

NOU:	Natoc_2649

HLR:	HALLA_07255

HLC:	CHINAEXTREME09050

NAJ:	B1756_14500

NAG:	AArcMg_2847

NAN:	AArc1_0855

NBG:	DV706_10780

NAS:	GCU68_01300

HDA:	BB347_08850

SAWL:

NGM29_06990

NAC:	AQV86_00615

MEAM:

MU439_01745

TAC:

Ta1136

TVO:	TVG1271204(TVG1271204)

PTO:

PTO0786

FAC:	FACI_IFERC01G1211

FAI:

FAD_0235

CDIV:

CPM_1322

TAR:	TALC_01514

MAX:	MMALV_16370

MER:	MMINT_19550

MEAR:

Mpt1_c13780(aglB)

MEUZ:

KRP56_00005

MARC:

AR505_0061

ABI:

Aboo_0310

ACF:	AciM339_0753

MARH:

Mia14_0132

FLT:	Sv326_1310

MIY:	Micr_00082 Micr_00288

NCON:

LC1Nh_1167(stt3)

AGW:	QT03_C0001G0537

ARG:	QT11_C0001G0576

» show all

Reference

1 [PMID:16824110]

Authors

Chaban B, Voisin S, Kelly J, Logan SM, Jarrell KF

Title

Identification of genes involved in the biosynthesis and attachment of Methanococcus voltae N-linked glycans: insight into N-linked glycosylation pathways in Archaea.

Journal

Mol Microbiol 61:259-68 (2006)
DOI:10.1111/j.1365-2958.2006.05226.x

Sequence

[ag:ABD17750]

Reference

2 [PMID:23624439]

Authors

Larkin A, Chang MM, Whitworth GE, Imperiali B

Title

Biochemical evidence for an alternate pathway in N-linked glycoprotein biosynthesis.

Journal

Nat Chem Biol 9:367-73 (2013)
DOI:10.1038/nchembio.1249