KEGG   ENZYME: 2.4.99.21
Entry
EC 2.4.99.21                Enzyme                                 

Name
dolichyl-phosphooligosaccharide-protein glycotransferase;
AglB;
archaeal oligosaccharyl transferase;
dolichyl-monophosphooligosaccharide-protein glycotransferase
Class
Transferases;
Glycosyltransferases;
Transferring other glycosyl groups
Sysname
dolichyl-phosphooligosaccharide:protein-L-asparagine N-beta-D-oligosaccharidotransferase
Reaction(IUBMB)
an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Substrate
archaeal dolichyl phosphooligosaccharide;
[protein]-L-asparagine
Product
archaeal dolichyl phosphate [CPD:C21212];
glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Comment
The enzyme, characterized from the archaea Methanococcus voltae and Haloferax volcanii, transfers a glycan component from dolichyl phosphooligosaccharide to external proteins. It is different from EC 2.4.99.18, dolichyl-diphosphooligosaccharide-protein glycotransferase, which uses dolichyl diphosphate as carrier compound in bacteria and eukaryotes. The enzyme participates in the N-glycosylation of proteins in some archaea. It requires Mn2+. Dolichol used by archaea is different from that used by eukaryotes. It is much shorter (C55-C60), it is alpha,omega-saturated and it may have additional unsaturated positions in the chain.
History
EC 2.4.99.21 created 2015
Orthology
K21306  dolichyl-phosphooligosaccharide-protein glycotransferase
Genes
GEO: Geob_1424 Geob_2990
DLI: dnl_42750
SUR: STAUR_5887
AGE: AA314_03686
AVM: JQX13_32570
CFUS: CYFUS_005592
VAI: BU251_01235
CTM: Cabther_A0306
CVL: J8C06_02030
CHLO: J8C02_01495
MJA: MJ_1525
MESG: MLAUSG7_0298(aglB)
MMP: MMP1424
MMD: GYY_07955
MMAD: MMJJ_14150
MAE: Maeo_1409
MVO: Mvol_1038
METE: tca_01572(pglB_2) tca_01834 tca_01844(pglB_3)
MST: Msp_0368
MRU: mru_0391
MSI: Msm_0716
MEB: Abm4_0186
MMIL: sm9_2154
MEYE: TL18_01100
MOL: YLM1_0063
MCUB: MCBB_1451 MCBB_1452(aglB_1) MCBB_1453(aglB_3) MCBB_1727
FPL: Ferp_2437
GAC: GACE_1401
GAH: GAH_01455
PHO: PH0242(PH0242) PH1271(PH1271)
MBU: Mbur_1579
MMET: MCMEM_2091
MMH: Mmah_0123
MPY: Mpsy_2355
MLA: Mlab_0662
MEMA: MMAB1_1829
MPL: Mpal_0785
HAL: VNG_1068G(tot)
HSL: OE_2548F(aglB)
HHB: Hhub_1167 Hhub_2271(aglB)
HALH: HTSR_0845(aglB)
HHSR: HSR6_0872(aglB)
HSU: HLASF_1520(aglB)
HSF: HLASA_1507(aglB)
HMA: rrnAC0431(tot)
HHI: HAH_1202(stt3)
NPH: NP_3720A(aglB)
NMO: Nmlp_2363(aglB)
HMU: Hmuk_2752
HALL: LC1Hm_2096(aglB)
HWA: HQ_2681A(aglB)
HWC: Hqrw_3013(aglB) Hqrw_3360
HVO: HVO_1530(aglB)
HME: HFX_1592(stt3)
HLA: Hlac_1062
HDF: AArcSl_2614(aglB2)
HTU: Htur_2957
NMG: Nmag_0927(aglB)
NAT: NJ7G_1722
SALI: L593_04990
TAC: Ta1136
TVO: TVG1271204(TVG1271204)
PTO: PTO0786
FAI: FAD_0235
CDIV: CPM_1322
MEAR: Mpt1_c13780(aglB)
MARC: AR505_0061
ABI: Aboo_0310
NAA: Nps_01945
MARH: Mia14_0132
NCON: LC1Nh_1167(stt3)
 » show all
Reference
1  [PMID:16824110]
  Authors
Chaban B, Voisin S, Kelly J, Logan SM, Jarrell KF
  Title
Identification of genes involved in the biosynthesis and attachment of Methanococcus voltae N-linked glycans: insight into N-linked glycosylation pathways in Archaea.
  Journal
Mol Microbiol 61:259-68 (2006)
DOI:10.1111/j.1365-2958.2006.05226.x
  Sequence
Reference
2  [PMID:23624439]
  Authors
Larkin A, Chang MM, Whitworth GE, Imperiali B
  Title
Biochemical evidence for an alternate pathway in N-linked glycoprotein biosynthesis.
  Journal
Nat Chem Biol 9:367-73 (2013)
DOI:10.1038/nchembio.1249
  Sequence
Reference
3  [PMID:24212570]
  Authors
Cohen-Rosenzweig C, Guan Z, Shaanan B, Eichler J
  Title
Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process  a variety of lipid-linked glycans.
  Journal
Appl Environ Microbiol 80:486-96 (2014)
DOI:10.1128/AEM.03191-13
Other DBs
ExplorEnz - The Enzyme Database: 2.4.99.21
IUBMB Enzyme Nomenclature: 2.4.99.21
ExPASy - ENZYME nomenclature database: 2.4.99.21
BRENDA, the Enzyme Database: 2.4.99.21

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