KEGG   ENZYME: 2.5.1.111Help
Entry
EC 2.5.1.111                Enzyme                                 

Name
4-hydroxyphenylpyruvate 3-dimethylallyltransferase;
CloQ;
4HPP dimethylallyltransferase;
NovQ
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
dimethylallyl diphosphate:4-hydroxyphenylpyruvate 3-dimethylallyltransferase
Reaction(IUBMB)
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate = diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate [RN:R06757]
Reaction(KEGG)
Substrate
dimethylallyl diphosphate [CPD:C00235];
4-hydroxyphenylpyruvate [CPD:C01179]
Product
diphosphate [CPD:C00013];
3-dimethylallyl-4-hydroxyphenylpyruvate [CPD:C12456]
Comment
The enzyme is involved in the biosynthesis of the 3-dimethylallyl-4-hydroxyphenylpyruvate moiety of the aminocoumarin antibiotics clorobiocin and novobiocin [1].
History
EC 2.5.1.111 created 2013
Pathway
ec00401  Novobiocin biosynthesis
ec01110  Biosynthesis of secondary metabolites
ec01130  Biosynthesis of antibiotics
Orthology
K12707  4-hydroxyphenylpyruvate 3-dimethylallyltransferase
Genes
NGV: CDO52_02230
Taxonomy
Reference
1  [PMID:12618544]
  Authors
Pojer F, Wemakor E, Kammerer B, Chen H, Walsh CT, Li SM, Heide L.
  Title
CloQ, a prenyltransferase involved in clorobiocin biosynthesis.
  Journal
Proc Natl Acad Sci U S A 100:2316-21 (2003)
DOI:10.1073/pnas.0337708100
  Sequence
Reference
2  [PMID:17077503]
  Authors
Keller S, Pojer F, Heide L, Lawson DM
  Title
Crystallization and preliminary X-ray analysis of the aromatic prenyltransferase  CloQ from the clorobiocin biosynthetic cluster of Streptomyces roseochromogenes.
  Journal
Acta Crystallogr Sect F Struct Biol Cryst Commun 62:1153-5 (2006)
DOI:10.1107/S1744309106042527
Reference
3  [PMID:20946900]
  Authors
Metzger U, Keller S, Stevenson CE, Heide L, Lawson DM
  Title
Structure and mechanism of the magnesium-independent aromatic prenyltransferase CloQ from the clorobiocin biosynthetic pathway.
  Journal
J Mol Biol 404:611-26 (2010)
DOI:10.1016/j.jmb.2010.09.067
  Sequence
Reference
4  [PMID:19557032]
  Authors
Ozaki T, Mishima S, Nishiyama M, Kuzuyama T
  Title
NovQ is a prenyltransferase capable of catalyzing the addition of a dimethylallyl group to both phenylpropanoids and flavonoids.
  Journal
J Antibiot (Tokyo) 62:385-92 (2009)
DOI:10.1038/ja.2009.48
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.111
IUBMB Enzyme Nomenclature: 2.5.1.111
ExPASy - ENZYME nomenclature database: 2.5.1.111
BRENDA, the Enzyme Database: 2.5.1.111

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