KEGG   ENZYME: 2.5.1.113
Entry
EC 2.5.1.113                Enzyme                                 
Name
[CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase;
CysM
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
O-phospho-L-serine:thiocarboxylated [CysO sulfur-carrier protein] 2-amino-2-carboxyethyltransferase
Reaction(IUBMB)
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH = [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate [RN:R10610]
Reaction(KEGG)
R10610
Substrate
O-phospho-L-serine [CPD:C01005];
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH [CPD:C15814]
Product
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine [CPD:C20729];
phosphate [CPD:C00009]
Comment
A pyridoxal-phosphate protein. The enzyme participates in an alternative pathway for L-cysteine biosynthesis that involves a protein-bound thiocarboxylate as the sulfide donor. The enzyme from the bacterium Mycobacterium tuberculosis also has very low activity with O3-acetyl-L-serine (cf. EC 2.5.1.65, O-phosphoserine sulfhydrylase).
History
EC 2.5.1.113 created 2013
Orthology
K21148  [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase
Genes
BEXA11Q_332
MTURv1336(cysM)
MTVRVBD_1336
MTCMT1377(cysM)
MRAMRA_1344(cysM)
MTFTBFG_11366
MTBTBMG_02642
MTKTBSG_02656
MTZTBXG_002622
MTGMRGA327_08375
MTECCDC5079_1237
MTURCFBS_1421(cysM)
MTLCCDC5180_1228
MTOMTCTRI2_1373(cysM)
MTDUDA_1336(cysM)
MTNERDMAN_1496(cysM)
MTJJ112_07205
MTUBMT7199_1368
MTUCJ113_09305
MTUEJ114_07185
MTXM943_07005
MTULTBHG_01323
MTUTHKBT1_1420(cysM)
MTUUHKBT2_1426(cysM)
MTQHKBS1_1424(cysM)
MBOBQ2027_MB1371(cysM)
MBBBCG_1398(cysM)
MBTJTY_1372(cysM)
MBMBCGMEX_1370(cysM)
MBKK60_014350
MBXBCGT_1171
MAFMAF_13600(cysM)
MMICRN08_1495
MCEMCAN_13541(cysM)
MCQBN44_11502(cysM)
MCVBN43_30435(cysM)
MCXBN42_21245(cysM)
MCZBN45_30418(cysM)
MSAMycsm_04617
MULMUL_0242(cysM)
MMCMmcs_3848
MKMMkms_3922
MJLMjls_3834
MMIMMAR_4629(cysM)
MMAEMMARE11_44530(cysM)
MLIMULP_04839(cysM)
MKNMKAN_06205
MYVG155_21135(cysM)
MYEAB431_22045(cysM)
MGOAFA91_15975(cysM)
MHADB586_08340(cysM)
MDXBTO20_10025(cysM)
MFJMFLOJ_50330(cysM)
MGROFZ046_24910
MXEMYXE_16650(cysM)
MNVMNVI_03280(cysM)
MBAIMB901379_03526(cysM)
MPSEMPSD_47870(cysM)
MSHOMSHO_22410(cysM)
MHEKJMUB5695_01732(cysM)
MGAUMGALJ_26840(cysM)
MLJMLAC_10040(cysM)
MBRDMBRA_31190(cysM)
MSHJMSHI_05610(cysM)
MKRMKOR_08390
MDFK0O62_20960
MMAMK3U93_17360
MHOLK3U96_07710
MSENK3U95_08760
MSPGF6B93_16225
MOTLTS72_05035
MRFMJO55_19470
MSMMSMEG_4905(cysM)
MSGMSMEI_4778
MSBLJ00_24255(cysM)
MSNLI99_24260(cysM)
MSHLI98_24265(cysM)
MVAMvan_4291
MGIMflv_2354
MSPMspyr1_17870
MCBMycch_3736
MNED174_20015(cysM)
MYNMyAD_19640(cysM)
MFTXA26_43140
MPHLMPHLCCUG_03882(cysM)
MVQMYVA_4106(cysM)
MRHMycrhN_3866
MTHN4412656_03139(cysM)
MHASMHAS_02665(cysM)
MDUMDUV_03800(cysM)
MDRMDOR_27070(cysM)
MAUUNCTC10437_04035(cysM)
MMORMMOR_22560(cysM)
MALVMALV_51390(cysM)
MTYMTOK_00950
MPSCMPSYJ_25300(cysM)
MARZMARA_57190(cysM)
MGADMGAD_55760(cysM)
MHEVMHEL_06120(cysM)
MSARMSAR_30230
MANYMANY_22430(cysM)
MPOFMPOR_43880(cysM)
MMATMMAGJ_01740(cysM)
MBOKMBOE_30700(cysM)
MSEIMSEDJ_51670
MFLVNCTC10271_01413(cysM)
MCEEMCEL_47020(cysM)
MABMAB_1480
MMVMYCMA_0803
MABBMASS_1472
MABLMMASJCM_1499
MCHEBB28_07370(cysM)
MIZBAB75_07995(cysM)
MSTEMSTE_01457
MSAOMYCSP_06580(cysM)
MSALDSM43276_01343(cysM)
ASDAS9A_1109
MPAKMIU77_08560
NFANFA_10910(cysM)
NFRERS450000_03020(cysM_1)
NCYNOCYR_1176(cysM)
NBRO3I_006480
NNONONO_c12910(cysM) NONO_c40660
NSLBOX37_25295(cysM)
NSRNS506_04798(cysM) NS506_07155
NTPCRH09_07265(cysM)
NOZDMB37_27415(cysM)
NODFOH10_24795
NAHF5544_07735 F5544_21065
NADNCTC11293_01987(cysM_1)
NWLNWFMUON74_59190
NIEKV110_07070 KV110_20310
NTCKHQ06_37850
NHUH0264_37005
NYALTV02_09555 LTV02_36215
NGPLTT66_05890
RHARHA1_ro01436(cysM)
ROPROP_11430(cysM) ROP_11450(cysM)
ROAPd630_LPD05558 Pd630_LPD05560
RHWBFN03_11000(cysM)
RTMG4H71_02325
RKOJWS14_07380 JWS14_07390
ROZCBI38_10795(cysM)
GBRGbro_1967
GPOGPOL_c18620(cysM)
GORKTR9_1899
GOQACH46_07410(cysM)
GTABCM27_10205(cysM)
GRUGCWB2_09585(cysM2)
GOMD7316_00476(cysM)
GAVC5O27_18170(cysM)
GODGKZ92_09190
GAMGII34_08840
GPDGII33_08230
GJIH1R19_09475
GOILK459_00855
GHNMVF96_09600
GAMIIHQ52_12480
SPINKV203_05210
TPRTpau_1291
TSMASU32_07345(cysM)
TPULTPB0596_32920
SCOSCO2910(SCE19A.10c)
SALBXNR_1784
SMASAVERM_5164(cysM2)
SGRSGR_4632
SGBWQO_12370(cysM)
SCBSCAB_56551 SCAB_86101
SSXSACTE_0751 SACTE_2387
SVLStrvi_3366 Strvi_8262
SHYSHJG_4386
SHOSHJGH_4149
SRCM271_30300
SMALSMALA_2974
SSOII1A49_18795
SCTSCAT_1941(cysM)
SCYSCATT_19320
SFASfla_3986
SBHSBI_02244(cysM2) SBI_06684(cysM3)
SVESVEN_2662
SDVBN159_5378(cysM)
SALSSLNWT_2450
STRPF750_2736
SFISFUL_2504
SCIB446_15210
SALUDC74_3343
SALLSAZ_17810
SLVSLIV_23140(cysM)
SGUSGLAU_13170(cysM)
SVTSVTN_14255(cysM)
STREGZL_01160 GZL_05560
SCWTU94_12665(cysM)
SLDT261_5208
SLCSL103_00750 SL103_04775(cysM)
SXISXIM_39020
STRMM444_13795(cysM)
STRCAA958_10735(cysM)
SAMBSAM23877_0667 SAM23877_2947(cysM)
SPRISPRI_4639
SCZABE83_23465(cysM)
SCXAS200_27885(cysM)
SRWTUE45_03502(cysM)
STRFASR50_01840 ASR50_14080(cysM)
SLEsle_43610(sle_43610)
SRNA4G23_01928(cysM_1) A4G23_04707(cysK)
SPAVSpa2297_12450(cysM)
STRTA8713_11700(cysM)
SCLFBB341_18215(cysM)
SGSAVL59_42290(cysM)
STSIA4E84_15310(cysM)
SKYD0C37_10680
SALWCP975_13075
SGMGCM10017557_51680 GCM10017557_72480
SCADDN051_24120(cysM)
SLSSLINC_3312
SNRSNOUR_25975
SPLULK06_011185(cysM)
STRDNI25_24205(cysM)
SNWBBN63_22260(cysM)
SAUOBV401_18535(cysM)
SSIAA7J05_22375(cysM)
SVUB1H20_11625(cysM)
SPUNBFF78_25945(cysM)
SGVB1H19_16130(cysM)
SLAUSLA_2642
SALFSMD44_03215(cysM)
SALJSMD11_4299(cysM)
SLXSLAV_23445(cysM2)
STROSTRMOE7_15100(cysM) STRMOE7_19095
SFKKY5_3048c
SNZDC008_12820(cysM)
SGEDWG14_05208(cysM)
SRJSRO_4572
SLKSLUN_14885(cysM)
SDXC4B68_25130(cysM)
SGDELQ87_07590 ELQ87_24040
SQZFQU76_10805
SCYAEJ357_17420
SASTCD934_20820(cysM) CD934_30860
SNQCP978_04895 CP978_13425
STIRDDW44_09560(cysM)
SKACP970_27280
SGZC0216_26580(cysM)
SVNCP980_02895 CP980_21065
SNKCP967_21745
SHAWCEB94_15840(cysM)
SRKFGW37_12000
SFICEIZ62_20880 EIZ62_22970
SGALCP966_13855
SSPODDQ41_07955(cysM) DDQ41_28910
SVRCP971_21215
SPADDVK44_10510
SFYGFH48_25075
SAQUEJC51_19845
SGFHEP81_04911
SCAVCVT27_11085(cysM)
SSEOD0Z67_10705
SBYH7H31_22965
SRIMCP984_25625
SSUBCP968_20865
SPHVF9278_17455
SCINCP977_12945
SFUGCNQ36_13190(cysM)
SPACB1H29_22940(cysM) B1H29_35220
STSUB7R87_10460(cysM)
SCHACP983_26745 CP983_38750
SGXH4W23_15420
SCOECP976_16985
SBATG4Z16_22140
SSPBCP982_17220
SNFJYK04_03503(cysM)
SPLACP981_14760
SBROGQF42_18205
STUIGCM10017668_26010
SATAC5746_15780(cysM)
SCHFIPT68_14110
SHUNDWB77_04794(cysM)
SHARHUT13_19500
SCYGS1361_15730(cysM2)
SFEUIM697_06540
SLIAHA039_22145
SDWK7C20_13810
SVDCP969_15615
SCALI6J39_11825
SAUHSU9_012335
SFBCP974_10730 CP974_25235
SMOBJ7W19_11805
SPRACP972_13910
SPEUCGZ69_13080(cysM)
SROIIAG44_25555
SXNIAG42_21845
SGJIAG43_11625
SHKJ2N69_13285
SANUK7396_22805
STRYEQG64_11625
SDDD9753_21950
SACTDMT42_15225(cysM)
SMAOCAG99_09815 CAG99_17795(cysM)
SGOBtest1122_07890
SINEKI385_17825
SLFJEQ17_29055
SDECL3078_17665
SAOVG3H79_23265
KSKKSE_28250(cysM)
KABB7C62_11275(cysM)
KAUB6264_15885(cysM)
KITCFP65_2632
STRIC7M71_010095 C7M71_029405
YIALO772_22060
MPHMLP_38890(cysM)
MIKFOE78_07660
MICGGJV80_00370
NCANoca_1312
NDKI601_2313(cysM)
NOYEXE57_17285
NOIFCL41_04360
NOOFE634_04935
NDPE2C04_05115
NSNEXE58_02090
NBEBack2_09640
NANOG5V58_03455
NMESH9L09_03400
NAROCFH99_07565(cysM)
NPIG7071_00050
NAQUENKNEFLB_03479(cysM)
NROK8W59_14820
NOQLN652_07300
NCQK6T13_12975
NPCKUV85_00960
NPSKRR39_13930
PSIMKR76_08150
AERAERYTH_05540(cysM)
AEZC3E78_04875(cysM)
AEBC6I20_10850(cysM)
AEFGEV26_04685
MUZH4N58_14990
KFLKfla_1841
KQIF1D05_29010
TFUTfu_2368
THAONI17_014740
TALXFOF52_12040
NDANdas_0383
NALB005_3251(cysM)
NGVCDO52_22910(cysM)
NEXNE857_08160
STRREKD16_05490(cysM)
SAIUJ4H86_11110
TCUTcur_3871
ACTWF7P10_27500
AGRAAGRA3207_002605
SROSros_1728
NOABKM31_36130(cysM)
NOWGBF35_08935
NCXNocox_08295(cysM)
NGNLCN96_09295
TBITbis_0938
FRAFrancci3_0868
FREFranean1_5652
FRIFraEuI1c_5851
FALFRAAL1500(cysM)
FSYFsymDg_1244
ACEAcel_1687
NMLNamu_1698
GOBGobs_1358
BSDBLASA_1168(cysM)
BLAPMVA48_09490
MMARMODMU_1392(cysM)
SENSACE_1206(cysM)
SACGFDZ84_17715
SVISvir_29160
SACCEYD13_16125(cysM)
AMDAMED_7711(cysK)
AMNRAM_39620
AMMAMES_7594(cysK)
AMZB737_7594(cysK)
AOIAORI_6492(cysM)
AJAAJAP_06505(cysM)
AMQAMETH_1416(cysK)
AMYCCU254_31020(cysM)
AMYBBKN51_25915(cysM)
AABA4R43_36335(cysM)
AMYYYIM_41670(cysM2)
AORISD37_07950(cysM)
AACDLWP59_06910
APRTMUY14_45660
PDXPsed_1511
PSEAWY02_22440(cysM)
PSEEFRP1_20220(cysM)
PSEHXF36_03570(cysM)
PSEQAD006_27635(cysM)
PECQAD017_07230(cysM)
PHHAFB00_18205(cysM)
PAUTPdca_14350(cysK_1)
PBROHOP40_01785
APRECNX65_05080(cysM)
AMIAmir_1011
SESPBN6_11660(cysM)
SSYIEKG83_05910
KALKALB_1251
KPHYAOZ06_09770(cysM)
LEDBBK82_06115(cysM)
AHMTL08_06500
ACTIUA75_06225
ACADUA74_06225
AHGAHOG_05965(cysM)
ACTAC1701_06770(cysM)
ALOCRK62183
PMADBAY61_25245(cysM)
SACEGIY23_05345
ACTUActkin_01153(cysM)
STPStrop_1102
SAQSare_0991
MAUMicau_1158
MILML5_1421
MICBMicB006_1190
MTUACSH63_18740(cysM)
MICHFJK98_05905
MTEMGCE86_00075
MCABHXZ27_07125
MSAGGCM10017556_37040
VMAVAB18032_10380
MCRAID554_06140
ASEACPL_1167(cysM)
AMSAMIS_9430
ACTNL083_1276
AFSAFR_06350
ACTSACWT_1048
AIHAiant_53310
ACTRAsp14428_34510
PLKCIK06_06060(cysM)
PLABC6361_13080(cysM)
PLATC6W10_12325(cysM)
PFLAPflav_060700
PSUUPsuf_022490
VERHUT12_06885
ATLAthai_51060(cysK)
ASERAsera_23780(cysK)
PRYPrubr_12250
CATICS0771_03790(cysK_1)
DVCDvina_48115
NHYJQS43_04175
CCAZCOUCH_33170
CAICaci_7559
SNASnas_1541
NAVJQS30_03005
GLYK3N28_07475
AEYCDG81_16665(cysM)
PLANA1s21148_01305(cysM)
PLAKA1s21155_01100(cysM)
PLIMPHILAsVB114_05915(cysM)
PSUFA1sIA56_01110(cysM)
PVSA1sIA79_01045(cysM)
JTLM6D93_06200
ABAIIMCC26256_111568
BSOLFSW04_21915
CWOCwoe_1846
PARNNBH00_12245
SBAEDSM104329_04137(cysM)
AYMYM304_27740(cysM)
ATQGH723_12050
AQZKSP35_08285
IAMHC251_15345
ERZER308_03705
EUZDVS28_a1371
KBSEPA93_12695 EPA93_42445
TBHTbon_07540 Tbon_08090
MOXDAMO_1028(cysK)
NACAQV86_04805
 » show all
Reference
1  [PMID:18842002]
  Authors
O'Leary SE, Jurgenson CT, Ealick SE, Begley TP
  Title
O-phospho-L-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis.
  Journal
Biochemistry 47:11606-15 (2008)
DOI:10.1021/bi8013664
  Sequence
[mtu:Rv1336]
Reference
2  [PMID:18771296]
  Authors
Jurgenson CT, Burns KE, Begley TP, Ealick SE
  Title
Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of Mycobacterium tuberculosis.
  Journal
Biochemistry 47:10354-64 (2008)
DOI:10.1021/bi800915j
  Sequence
[mtu:Rv1336]
Reference
3  [PMID:18799456]
  Authors
Agren D, Schnell R, Oehlmann W, Singh M, Schneider G
  Title
Cysteine synthase (CysM) of Mycobacterium tuberculosis is an O-phosphoserine sulfhydrylase: evidence for an alternative cysteine biosynthesis pathway in mycobacteria.
  Journal
J Biol Chem 283:31567-74 (2008)
DOI:10.1074/jbc.M804877200
  Sequence
[mtu:Rv1336]
Reference
4  [PMID:19101553]
  Authors
Agren D, Schnell R, Schneider G
  Title
The C-terminal of CysM from Mycobacterium tuberculosis protects the aminoacrylate intermediate and is involved in sulfur donor selectivity.
  Journal
FEBS Lett 583:330-6 (2009)
DOI:10.1016/j.febslet.2008.12.019
  Sequence
[mtu:Rv1336]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.113
IUBMB Enzyme Nomenclature: 2.5.1.113
ExPASy - ENZYME nomenclature database: 2.5.1.113
BRENDA, the Enzyme Database: 2.5.1.113

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