| Entry |
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| Name |
[CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase;
CysM
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| Class |
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
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| Sysname |
O-phospho-L-serine:thiocarboxylated [CysO sulfur-carrier protein] 2-amino-2-carboxyethyltransferase
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| Reaction(IUBMB) |
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH = [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate [RN: R10610]
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| Reaction(KEGG) |
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| Substrate |
O-phospho-L-serine [CPD: C01005];
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH [CPD: C15814]
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| Product |
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine [CPD: C20729];
phosphate [CPD: C00009]
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| Comment |
A pyridoxal-phosphate protein. The enzyme participates in an alternative pathway for L-cysteine biosynthesis that involves a protein-bound thiocarboxylate as the sulfide donor. The enzyme from the bacterium Mycobacterium tuberculosis also has very low activity with O3-acetyl-L-serine (cf. EC 2.5.1.65, O-phosphoserine sulfhydrylase).
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| History |
EC 2.5.1.113 created 2013
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| Orthology |
| K21148 | [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase |
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| Genes |
» show all
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| Reference |
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| Authors |
O'Leary SE, Jurgenson CT, Ealick SE, Begley TP |
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| Title |
O-phospho-L-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Jurgenson CT, Burns KE, Begley TP, Ealick SE |
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| Title |
Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of Mycobacterium tuberculosis. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Agren D, Schnell R, Oehlmann W, Singh M, Schneider G |
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| Title |
Cysteine synthase (CysM) of Mycobacterium tuberculosis is an O-phosphoserine sulfhydrylase: evidence for an alternative cysteine biosynthesis pathway in mycobacteria. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Agren D, Schnell R, Schneider G |
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| Title |
The C-terminal of CysM from Mycobacterium tuberculosis protects the aminoacrylate intermediate and is involved in sulfur donor selectivity. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 2.5.1.113 |
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