KEGG ENZYME: 2.5.1.113

ENZYME: 2.5.1.113

Entry

EC 2.5.1.113                Enzyme

Name

[CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase;
CysM

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

O-phospho-L-serine:thiocarboxylated [CysO sulfur-carrier protein] 2-amino-2-carboxyethyltransferase

Reaction(IUBMB)

O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH = [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate [RN:R10610]

Reaction(KEGG)

R10610

Substrate

O-phospho-L-serine [CPD:C01005];
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH [CPD:C15814]

Product

[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine [CPD:C20729];
phosphate [CPD:C00009]

Comment

A pyridoxal-phosphate protein. The enzyme participates in an alternative pathway for L-cysteine biosynthesis that involves a protein-bound thiocarboxylate as the sulfide donor. The enzyme from the bacterium Mycobacterium tuberculosis also has very low activity with O3-acetyl-L-serine (cf. EC 2.5.1.65, O-phosphoserine sulfhydrylase).

History

EC 2.5.1.113 created 2013

Orthology

K21148

[CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase

Genes

BEX:

A11Q_332

MTU:	Rv1336(cysM)

MTV:

RVBD_1336

MTC:	MT1377(cysM)

MRA:	MRA_1344(cysM)

MTF:	TBFG_11366

MTB:	TBMG_02642

MTK:	TBSG_02656

MTZ:	TBXG_002622

MTG:	MRGA327_08375

MTE:	CCDC5079_1237

MTUR:

CFBS_1421(cysM)

MTL:	CCDC5180_1228

MTO:	MTCTRI2_1373(cysM)

MTD:	UDA_1336(cysM)

MTN:	ERDMAN_1496(cysM)

MTJ:	J112_07205

MTUB:

MT7199_1368

MTUC:

J113_09305

MTUE:

J114_07185

MTX:	M943_07005

MTUL:

TBHG_01323

MTUT:

HKBT1_1420(cysM)

MTUU:

HKBT2_1426(cysM)

MTQ:	HKBS1_1424(cysM)

MBO:	BQ2027_MB1371(cysM)

MBB:	BCG_1398(cysM)

MBT:	JTY_1372(cysM)

MBM:	BCGMEX_1370(cysM)

MBK:	K60_014350

MBX:

BCGT_1171

MAF:	MAF_13600(cysM)

MMIC:

RN08_1495

MCE:	MCAN_13541(cysM)

MCQ:	BN44_11502(cysM)

MCV:	BN43_30435(cysM)

MCX:	BN42_21245(cysM)

MCZ:	BN45_30418(cysM)

MSA:	Mycsm_04617

MUL:	MUL_0242(cysM)

MMC:

Mmcs_3848

MKM:

Mkms_3922

MJL:

Mjls_3834

MMI:	MMAR_4629(cysM)

MMAE:

MMARE11_44530(cysM)

MLI:	MULP_04839(cysM)

MKN:	MKAN_06205

MYV:	G155_21135(cysM)

MYE:	AB431_22045(cysM)

MGO:	AFA91_15975(cysM)

MHAD:

B586_08340(cysM)

MDX:	BTO20_10025(cysM)

MFJ:	MFLOJ_50330(cysM)

MGRO:

FZ046_24910

MXE:	MYXE_16650(cysM)

MNV:	MNVI_03280(cysM)

MBAI:

MB901379_03526(cysM)

MPSE:

MPSD_47870(cysM)

MSHO:

MSHO_22410(cysM)

MHEK:

JMUB5695_01732(cysM)

MGAU:

MGALJ_26840(cysM)

MLJ:	MLAC_10040(cysM)

MBRD:

MBRA_31190(cysM)

MSHJ:

MSHI_05610(cysM)

MKR:	MKOR_08390

MDF:	K0O62_20960

MMAM:

K3U93_17360

MHOL:

K3U96_07710

MSEN:

K3U95_08760

MSPG:

F6B93_16225

MOT:	LTS72_05035

MRF:	MJO55_19470

MSM:	MSMEG_4905(cysM)

MSG:	MSMEI_4778

MSB:	LJ00_24255(cysM)

MSN:	LI99_24260(cysM)

MSH:	LI98_24265(cysM)

MVA:

Mvan_4291

MGI:

Mflv_2354

MSP:	Mspyr1_17870

MCB:	Mycch_3736

MNE:	D174_20015(cysM)

MYN:	MyAD_19640(cysM)

MFT:	XA26_43140

MPHL:

MPHLCCUG_03882(cysM)

MVQ:	MYVA_4106(cysM)

MRH:	MycrhN_3866

MTHN:

4412656_03139(cysM)

MHAS:

MHAS_02665(cysM)

MDU:	MDUV_03800(cysM)

MDR:	MDOR_27070(cysM)

MAUU:

NCTC10437_04035(cysM)

MMOR:

MMOR_22560(cysM)

MALV:

MALV_51390(cysM)

MTY:	MTOK_00950

MPSC:

MPSYJ_25300(cysM)

MARZ:

MARA_57190(cysM)

MGAD:

MGAD_55760(cysM)

MHEV:

MHEL_06120(cysM)

MSAR:

MSAR_30230

MANY:

MANY_22430(cysM)

MPOF:

MPOR_43880(cysM)

MMAT:

MMAGJ_01740(cysM)

MBOK:

MBOE_30700(cysM)

MSEI:

MSEDJ_51670

MFLV:

NCTC10271_01413(cysM)

MCEE:

MCEL_47020(cysM)

MAB:

MAB_1480

MMV:	MYCMA_0803

MABB:

MASS_1472

MABL:

MMASJCM_1499

MCHE:

BB28_07370(cysM)

MIZ:	BAB75_07995(cysM)

MSTE:

MSTE_01457

MSAO:

MYCSP_06580(cysM)

MSAL:

DSM43276_01343(cysM)

ASD:

AS9A_1109

MPAK:

MIU77_08560

NFA:	NFA_10910(cysM)

NFR:	ERS450000_03020(cysM_1)

NCY:	NOCYR_1176(cysM)

NBR:	O3I_006480

NNO:	NONO_c12910(cysM) NONO_c40660

NSL:	BOX37_25295(cysM)

NSR:	NS506_04798(cysM) NS506_07155

NTP:	CRH09_07265(cysM)

NOZ:	DMB37_27415(cysM)

NOD:	FOH10_24795

NAH:	F5544_07735 F5544_21065

NAD:	NCTC11293_01987(cysM_1)

NWL:	NWFMUON74_59190

NIE:	KV110_07070 KV110_20310

NTC:	KHQ06_37850

NHU:	H0264_37005

NYA:	LTV02_09555 LTV02_36215

NGP:	LTT66_05890

RHA:	RHA1_ro01436(cysM)

ROP:	ROP_11430(cysM) ROP_11450(cysM)

ROA:	Pd630_LPD05558 Pd630_LPD05560

RHW:	BFN03_11000(cysM)

RTM:	G4H71_02325

RKO:	JWS14_07380 JWS14_07390

ROZ:	CBI38_10795(cysM)

GBR:

Gbro_1967

GPO:	GPOL_c18620(cysM)

GOR:

KTR9_1899

GOQ:	ACH46_07410(cysM)

GTA:	BCM27_10205(cysM)

GRU:	GCWB2_09585(cysM2)

GOM:	D7316_00476(cysM)

GAV:	C5O27_18170(cysM)

GOD:	GKZ92_09190

GAM:	GII34_08840

GPD:	GII33_08230

GJI:	H1R19_09475

GOI:	LK459_00855

GHN:	MVF96_09600

GAMI:

IHQ52_12480

SPIN:

KV203_05210

TPR:

Tpau_1291

TSM:	ASU32_07345(cysM)

TPUL:

TPB0596_32920

SCO:	SCO2910(SCE19A.10c)

SALB:

XNR_1784

SMA:	SAVERM_5164(cysM2)

SGR:

SGR_4632

SGB:	WQO_12370(cysM)

SCB:	SCAB_56551 SCAB_86101

SSX:	SACTE_0751 SACTE_2387

SVL:	Strvi_3366 Strvi_8262

SHY:

SHJG_4386

SHO:	SHJGH_4149

SRC:	M271_30300

SMAL:

SMALA_2974

SSOI:

I1A49_18795

SCT:	SCAT_1941(cysM)

SCY:	SCATT_19320

SFA:

Sfla_3986

SBH:	SBI_02244(cysM2) SBI_06684(cysM3)

SVE:

SVEN_2662

SDV:	BN159_5378(cysM)

SALS:

SLNWT_2450

STRP:

F750_2736

SFI:

SFUL_2504

SCI:	B446_15210

SALU:

DC74_3343

SALL:

SAZ_17810

SLV:	SLIV_23140(cysM)

SGU:	SGLAU_13170(cysM)

SVT:	SVTN_14255(cysM)

STRE:

GZL_01160 GZL_05560

SCW:	TU94_12665(cysM)

SLD:

T261_5208

SLC:	SL103_00750 SL103_04775(cysM)

SXI:	SXIM_39020

STRM:

M444_13795(cysM)

STRC:

AA958_10735(cysM)

SAMB:

SAM23877_0667 SAM23877_2947(cysM)

SPRI:

SPRI_4639

SCZ:	ABE83_23465(cysM)

SCX:	AS200_27885(cysM)

SRW:	TUE45_03502(cysM)

STRF:

ASR50_01840 ASR50_14080(cysM)

SLE:	sle_43610(sle_43610)

SRN:	A4G23_01928(cysM_1) A4G23_04707(cysK)

SPAV:

Spa2297_12450(cysM)

STRT:

A8713_11700(cysM)

SCLF:

BB341_18215(cysM)

SGS:	AVL59_42290(cysM)

STSI:

A4E84_15310(cysM)

SKY:	D0C37_10680

SALW:

CP975_13075

SGM:	GCM10017557_51680 GCM10017557_72480

SCAD:

DN051_24120(cysM)

SLS:	SLINC_3312

SNR:	SNOUR_25975

SPLU:

LK06_011185(cysM)

STRD:

NI25_24205(cysM)

SNW:	BBN63_22260(cysM)

SAUO:

BV401_18535(cysM)

SSIA:

A7J05_22375(cysM)

SVU:	B1H20_11625(cysM)

SPUN:

BFF78_25945(cysM)

SGV:	B1H19_16130(cysM)

SLAU:

SLA_2642

SALF:

SMD44_03215(cysM)

SALJ:

SMD11_4299(cysM)

SLX:	SLAV_23445(cysM2)

STRO:

STRMOE7_15100(cysM) STRMOE7_19095

SFK:

KY5_3048c

SNZ:	DC008_12820(cysM)

SGE:	DWG14_05208(cysM)

SRJ:

SRO_4572

SLK:	SLUN_14885(cysM)

SDX:	C4B68_25130(cysM)

SGD:	ELQ87_07590 ELQ87_24040

SQZ:	FQU76_10805

SCYA:

EJ357_17420

SAST:

CD934_20820(cysM) CD934_30860

SNQ:	CP978_04895 CP978_13425

STIR:

DDW44_09560(cysM)

SKA:	CP970_27280

SGZ:	C0216_26580(cysM)

SVN:	CP980_02895 CP980_21065

SNK:	CP967_21745

SHAW:

CEB94_15840(cysM)

SRK:	FGW37_12000

SFIC:

EIZ62_20880 EIZ62_22970

SGAL:

CP966_13855

SSPO:

DDQ41_07955(cysM) DDQ41_28910

SVR:	CP971_21215

SPAD:

DVK44_10510

SFY:	GFH48_25075

SAQU:

EJC51_19845

SGF:	HEP81_04911

SCAV:

CVT27_11085(cysM)

SSEO:

D0Z67_10705

SBY:	H7H31_22965

SRIM:

CP984_25625

SSUB:

CP968_20865

SPHV:

F9278_17455

SCIN:

CP977_12945

SFUG:

CNQ36_13190(cysM)

SPAC:

B1H29_22940(cysM) B1H29_35220

STSU:

B7R87_10460(cysM)

SCHA:

CP983_26745 CP983_38750

SGX:	H4W23_15420

SCOE:

CP976_16985

SBAT:

G4Z16_22140

SSPB:

CP982_17220

SNF:	JYK04_03503(cysM)

SPLA:

CP981_14760

SBRO:

GQF42_18205

STUI:

GCM10017668_26010

SATA:

C5746_15780(cysM)

SCHF:

IPT68_14110

SHUN:

DWB77_04794(cysM)

SHAR:

HUT13_19500

SCYG:

S1361_15730(cysM2)

SFEU:

IM697_06540

SLIA:

HA039_22145

SDW:	K7C20_13810

SVD:	CP969_15615

SCAL:

I6J39_11825

SAUH:

SU9_012335

SFB:	CP974_10730 CP974_25235

SMOB:

J7W19_11805

SPRA:

CP972_13910

SPEU:

CGZ69_13080(cysM)

SROI:

IAG44_25555

SXN:	IAG42_21845

SGJ:	IAG43_11625

SHK:	J2N69_13285

SANU:

K7396_22805

STRY:

EQG64_11625

SDD:	D9753_21950

SACT:

DMT42_15225(cysM)

SMAO:

CAG99_09815 CAG99_17795(cysM)

SGOB:

test1122_07890

SINE:

KI385_17825

SLF:	JEQ17_29055

SDEC:

L3078_17665

SAOV:

G3H79_23265

KSK:	KSE_28250(cysM)

KAB:	B7C62_11275(cysM)

KAU:	B6264_15885(cysM)

KIT:	CFP65_2632

STRI:

C7M71_010095 C7M71_029405

YIA:	LO772_22060

MPH:	MLP_38890(cysM)

MIK:	FOE78_07660

MICG:

GJV80_00370

NCA:

Noca_1312

NDK:	I601_2313(cysM)

NOY:	EXE57_17285

NOI:	FCL41_04360

NOO:	FE634_04935

NDP:	E2C04_05115

NSN:	EXE58_02090

NBE:	Back2_09640

NANO:

G5V58_03455

NMES:

H9L09_03400

NARO:

CFH99_07565(cysM)

NPI:	G7071_00050

NAQU:

ENKNEFLB_03479(cysM)

NRO:	K8W59_14820

NOQ:	LN652_07300

NCQ:	K6T13_12975

NPC:	KUV85_00960

NPS:	KRR39_13930

PSIM:

KR76_08150

AER:	AERYTH_05540(cysM)

AEZ:	C3E78_04875(cysM)

AEB:	C6I20_10850(cysM)

AEF:	GEV26_04685

MUZ:	H4N58_14990

KFL:

Kfla_1841

KQI:	F1D05_29010

TFU:

Tfu_2368

THAO:

NI17_014740

TALX:

FOF52_12040

NDA:

Ndas_0383

NAL:	B005_3251(cysM)

NGV:	CDO52_22910(cysM)

NEX:	NE857_08160

STRR:

EKD16_05490(cysM)

SAIU:

J4H86_11110

TCU:

Tcur_3871

ACTW:

F7P10_27500

AGRA:

AGRA3207_002605

SRO:

Sros_1728

NOA:	BKM31_36130(cysM)

NOW:	GBF35_08935

NCX:	Nocox_08295(cysM)

NGN:	LCN96_09295

TBI:

Tbis_0938

FRA:	Francci3_0868

FRE:	Franean1_5652

FRI:	FraEuI1c_5851

FAL:	FRAAL1500(cysM)

FSY:	FsymDg_1244

ACE:

Acel_1687

NML:

Namu_1698

GOB:

Gobs_1358

BSD:	BLASA_1168(cysM)

BLAP:

MVA48_09490

MMAR:

MODMU_1392(cysM)

SEN:	SACE_1206(cysM)

SACG:

FDZ84_17715

SVI:	Svir_29160

SACC:

EYD13_16125(cysM)

AMD:	AMED_7711(cysK)

AMN:

RAM_39620

AMM:	AMES_7594(cysK)

AMZ:	B737_7594(cysK)

AOI:	AORI_6492(cysM)

AJA:	AJAP_06505(cysM)

AMQ:	AMETH_1416(cysK)

AMYC:

CU254_31020(cysM)

AMYB:

BKN51_25915(cysM)

AAB:	A4R43_36335(cysM)

AMYY:

YIM_41670(cysM2)

AORI:

SD37_07950(cysM)

AACD:

LWP59_06910

APRT:

MUY14_45660

PDX:

Psed_1511

PSEA:

WY02_22440(cysM)

PSEE:

FRP1_20220(cysM)

PSEH:

XF36_03570(cysM)

PSEQ:

AD006_27635(cysM)

PECQ:

AD017_07230(cysM)

PHH:	AFB00_18205(cysM)

PAUT:

Pdca_14350(cysK_1)

PBRO:

HOP40_01785

APRE:

CNX65_05080(cysM)

AMI:

Amir_1011

SESP:

BN6_11660(cysM)

SSYI:

EKG83_05910

KAL:

KALB_1251

KPHY:

AOZ06_09770(cysM)

LED:	BBK82_06115(cysM)

AHM:	TL08_06500

ACTI:

UA75_06225

ACAD:

UA74_06225

AHG:	AHOG_05965(cysM)

ACTA:

C1701_06770(cysM)

ALO:

CRK62183

PMAD:

BAY61_25245(cysM)

SACE:

GIY23_05345

ACTU:

Actkin_01153(cysM)

STP:	Strop_1102

SAQ:

Sare_0991

MAU:	Micau_1158

MIL:

ML5_1421

MICB:

MicB006_1190

MTUA:

CSH63_18740(cysM)

MICH:

FJK98_05905

MTEM:

GCE86_00075

MCAB:

HXZ27_07125

MSAG:

GCM10017556_37040

VMA:	VAB18032_10380

MCRA:

ID554_06140

ASE:	ACPL_1167(cysM)

AMS:

AMIS_9430

ACTN:

L083_1276

AFS:

AFR_06350

ACTS:

ACWT_1048

AIH:	Aiant_53310

ACTR:

Asp14428_34510

PLK:	CIK06_06060(cysM)

PLAB:

C6361_13080(cysM)

PLAT:

C6W10_12325(cysM)

PFLA:

Pflav_060700

PSUU:

Psuf_022490

VER:	HUT12_06885

ATL:	Athai_51060(cysK)

ASER:

Asera_23780(cysK)

PRY:	Prubr_12250

CATI:

CS0771_03790(cysK_1)

DVC:	Dvina_48115

NHY:	JQS43_04175

CCAZ:

COUCH_33170

CAI:

Caci_7559

SNA:

Snas_1541

NAV:	JQS30_03005

GLY:	K3N28_07475

AEY:	CDG81_16665(cysM)

PLAN:

A1s21148_01305(cysM)

PLAK:

A1s21155_01100(cysM)

PLIM:

PHILAsVB114_05915(cysM)

PSUF:

A1sIA56_01110(cysM)

PVS:	A1sIA79_01045(cysM)

JTL:	M6D93_06200

ABAI:

IMCC26256_111568

BSOL:

FSW04_21915

CWO:

Cwoe_1846

PARN:

NBH00_12245

SBAE:

DSM104329_04137(cysM)

AYM:	YM304_27740(cysM)

ATQ:	GH723_12050

AQZ:	KSP35_08285

IAM:	HC251_15345

ERZ:	ER308_03705

EUZ:	DVS28_a1371

KBS:	EPA93_12695 EPA93_42445

TBH:	Tbon_07540 Tbon_08090

MOX:	DAMO_1028(cysK)

NAC:	AQV86_04805

» show all

Reference

1 [PMID:18842002]

Authors

O'Leary SE, Jurgenson CT, Ealick SE, Begley TP

Title

O-phospho-L-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis.

Journal

Biochemistry 47:11606-15 (2008)
DOI:10.1021/bi8013664

Sequence

[mtu:Rv1336]

Reference

2 [PMID:18771296]

Authors

Jurgenson CT, Burns KE, Begley TP, Ealick SE

Title

Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of Mycobacterium tuberculosis.

Journal

Biochemistry 47:10354-64 (2008)
DOI:10.1021/bi800915j

Sequence

[mtu:Rv1336]

Reference

3 [PMID:18799456]

Authors

Agren D, Schnell R, Oehlmann W, Singh M, Schneider G

Title

Cysteine synthase (CysM) of Mycobacterium tuberculosis is an O-phosphoserine sulfhydrylase: evidence for an alternative cysteine biosynthesis pathway in mycobacteria.

Journal

J Biol Chem 283:31567-74 (2008)
DOI:10.1074/jbc.M804877200

Sequence

[mtu:Rv1336]

Reference

4 [PMID:19101553]

Authors

Agren D, Schnell R, Schneider G

Title

The C-terminal of CysM from Mycobacterium tuberculosis protects the aminoacrylate intermediate and is involved in sulfur donor selectivity.

Journal

FEBS Lett 583:330-6 (2009)
DOI:10.1016/j.febslet.2008.12.019