KEGG   ENZYME: 2.5.1.114
Entry
EC 2.5.1.114                Enzyme                                 
Name
tRNAPhe (4-demethylwyosine37-C7) aminocarboxypropyltransferase;
TYW2;
tRNA-yW synthesizing enzyme-2;
TRM12 (gene name);
taw2 (gene name)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
S-adenosyl-L-methionine:tRNAPhe (4-demethylwyosine37-C7)-[(3S)-3-amino-3-carboxypropyl]transferase
Reaction(IUBMB)
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe = S-methyl-5'-thioadenosine + 7-[(3S)-3-amino-3-carboxypropyl]-4-demethylwyosine37 in tRNAPhe [RN:R10611]
Reaction(KEGG)
R10611
Substrate
S-adenosyl-L-methionine [CPD:C00019];
4-demethylwyosine37 in tRNAPhe
Product
S-methyl-5'-thioadenosine [CPD:C00170];
7-[(3S)-3-amino-3-carboxypropyl]-4-demethylwyosine37 in tRNAPhe
Comment
The enzyme, which is found in all eukaryotes and in the majority of Euryarchaeota (but not in the Crenarchaeota), is involved in the hypermodification of the guanine nucleoside at position 37 of tRNA leading to formation of assorted wye bases. This modification is essential for translational reading-frame maintenance. The eukaryotic enzyme is involved in biosynthesis of the tricyclic base wybutosine, which is found only in tRNAPhe.
History
EC 2.5.1.114 created 2013
Orthology
K07055  tRNA wybutosine-synthesizing protein 2
Genes
HSA55039(TRMT12)
PTR464377(TRMT12)
PPS100979722(TRMT12)
GGO101124627(TRMT12)
PON100447332(TRMT12)
NLE100582652(TRMT12)
MCC705848(TRMT12)
MCF101865973(TRMT12)
CSAB103237404(TRMT12)
CATY105598680(TRMT12)
PANU101020238(TRMT12)
TGE112630768(TRMT12)
RRO104662794(TRMT12)
RBB108538090(TRMT12)
TFN117078877(TRMT12)
PTEH111550755(TRMT12)
CJC100385176(TRMT12)
SBQ101029907(TRMT12)
MMUR105884369(TRMT12)
LCAT123644437(TRMT12)
OGA100958117(TRMT12)
MMU68260(Trmt12)
MCAL110310681(Trmt12)
MPAH110335044(Trmt12)
RNO314999(Trmt12)
MCOC116082400(Trmt12)
MUN110552425(Trmt12)
CGE113837486
MAUA101832749(Trmt12)
PLEU114694812(Trmt12)
MORG121461067(Trmt12)
AAMP119823230(Trmt12)
NGI103743903(Trmt12)
HGL101715645(Trmt12)
CPOC100734337(Trmt12)
CCAN109688115(Trmt12)
DORD106001769(Trmt12)
DSP122102336(Trmt12)
OCU100352397(TRMT12)
OPI101519993(TRMT12)
TUP102500902(TRMT12)
CFA609411(TRMT12)
CLUD112662127(TRMT12)
VVP112915968(TRMT12)
VLG121498782(TRMT12)
AML100468536(TRMT12)
UMR103661618(TRMT12)
UAH113252745(TRMT12)
UAR123797720(TRMT12)
ELK111146607
LLV125096951
MPUF101681207(TRMT12)
ORO101384928(TRMT12)
EJU114211197(TRMT12)
ZCA113912492(TRMT12)
MLX118015195(TRMT12)
FCA101085181(TRMT12)
PYU121042954(TRMT12)
PBG122495795(TRMT12)
PTG102970870(TRMT12)
PPAD109273164(TRMT12)
AJU106986564(TRMT12)
HHV120231767(TRMT12)
BTA516516(TRMT12)
BOM102277834(TRMT12)
BIU109568571(TRMT12)
BBUB102406539(TRMT12)
CHX102189221(TRMT12)
OAS101106695(TRMT12)
ODA120878923(TRMT12)
CCAD122451232(TRMT12)
SSC100155406(TRMT12)
CFR102519623(TRMT12)
CBAI105082926(TRMT12)
CDK105096435(TRMT12)
VPC102539603(TRMT12)
BACU103011113(TRMT12)
LVE103086079(TRMT12)
OOR101277734(TRMT12)
DLE111164382(TRMT12)
PCAD102978377(TRMT12)
PSIU116742288(TRMT12)
ECB100067346(TRMT12)
EPZ103564519(TRMT12)
EAI106839357(TRMT12)
MYB106723536(TRMT12)
MYD102770501(TRMT12)
MMYO118670979(TRMT12)
MLF102424997(TRMT12)
MNA107539501(TRMT12)
PKL118718406(TRMT12)
DRO112315491(TRMT12)
SHON118988661(TRMT12)
AJM119064841(TRMT12)
PDIC114514494(TRMT12)
PHAS123833115(TRMT12)
MMF118631479(TRMT12)
RFQ117033556(TRMT12)
PALE102896712(TRMT12)
PGIG120595677(TRMT12)
PVP105292545(TRMT12)
RAY107521596(TRMT12)
MJV108392307(TRMT12)
TOD119249369(TRMT12)
SARA101544155(TRMT12)
LAV100658475(TRMT12)
TMU101347238
DNM101438799(TRMT12)
MDO100020165(TRMT12) 100023277
GAS123231784(TRMT12) 123255422
SHR100926005(TRMT12)
PCW110192303(TRMT12)
OAA100087951(TRMT12)
GGA422259(TRMT12)
PCOC116229661(TRMT12)
MGP100538997(TRMT12)
CJO107312716(TRMT12)
NMEL110403089(TRMT12)
APLA101799970(TRMT12)
ACYG106037483(TRMT12)
AFUL116494524(TRMT12)
TGU100226909(TRMT12)
LSR110469608(TRMT12)
SCAN103816584(TRMT12)
PMOA120508685(TRMT12)
OTC121331765(TRMT12)
PRUF121349119(TRMT12)
FAB101812632(TRMT12)
PHI102114676(TRMT12)
PMAJ107204035(TRMT12)
CCAE111928720(TRMT12)
CCW104691361(TRMT12)
ETL114060707(TRMT12)
ACHL103798427(TRMT12)
FPG101916683(TRMT12)
FCH102054438(TRMT12)
CLV102096517
EGZ104129953(TRMT12)
NNI104012948(TRMT12)
PLET104628371(TRMT12)
ACUN113480193(TRMT12)
TALA116959083(TRMT12)
ACHC115333649(TRMT12)
CCRI104162575(TRMT12)
EHS104515697(TRMT12)
CMAC104487697(TRMT12)
MUI104534454(TRMT12)
LDI104345837(TRMT12)
AAM106484769(TRMT12)
AROW112974121(TRMT12)
NPD112944484(TRMT12)
DNE112989740(TRMT12)
ASN102382316(TRMT12)
AMJ102563349(TRMT12)
CPOO109311027(TRMT12)
GGN109300623(TRMT12)
PSS102456684(TRMT12)
CMY102935765(TRMT12)
CPIC101946914(TRMT12)
TST117882682(TRMT12)
CABI116819983(TRMT12)
MRV120372374(TRMT12)
PVT110083587(TRMT12)
SUND121936220(TRMT12)
PBI103062147(TRMT12)
PMUR107291575(TRMT12)
TSR106551718(TRMT12)
PGUT117665119(TRMT12)
VKO123021966(TRMT12)
PMUA114589041(TRMT12)
ZVI118084422(TRMT12)
GJA107124532(TRMT12)
STOW125442370(TRMT12)
XLA108698285(trmt12.L)
XTR100380191(trmt12)
NPR108799572(TRMT12)
RTEM120913055(TRMT12)
BBUF120977596(TRMT12)
BGAR122919398(TRMT12)
DRE564925(trmt12)
SRX107740100(trmt12)
SANH107669089(trmt12)
SGH107602575(trmt12)
CCAR109079186
CAUA113113888(trmt12)
PPRM120474346(trmt12)
MAMB125259286(trmt12)
IPU108268801(trmt12)
PHYP113529183(trmt12)
SMEO124385898(trmt12)
TFD113636049(trmt12)
AMEX103042921(trmt12)
EEE113585203(trmt12)
TRU101061055(trmt12)
TNGGSTEN00031299G001
LCO104928726(trmt12)
CGOB115014594(trmt12)
ELY117260647(trmt12)
EFO125893990(trmt12)
PLEP121948109(trmt12)
SLUC116052301(trmt12)
ECRA117952138(trmt12)
ESP116696737(trmt12)
PFLV114562320(trmt12)
GAT120817855(trmt12)
PPUG119210930(trmt12)
MSAM119889307(trmt12)
CUD121515968(trmt12)
ALAT119007995(trmt12)
MZE101477717(trmt12)
ONL100692074(trmt12)
OAU116317612(trmt12)
OLA101163092(trmt12)
OML112153811(trmt12)
XMA102227936(trmt12)
XCO114138657(trmt12)
XHE116713895(trmt12)
PRET103471570(trmt12)
PFOR103150181(trmt12)
PLAI106945447(trmt12)
PMEI106915283(trmt12)
GAF122837506(trmt12)
CVG107093579(trmt12)
CTUL119786245(trmt12)
GMU124860136(trmt12)
NFU107378195(trmt12)
KMR108231272(trmt12)
ALIM106514856(trmt12)
NWH119408405(trmt12)
AOCE111582111(trmt12)
MCEP125008061(trmt12)
CSEM103390537(trmt12)
POV109625780(trmt12)
SSEN122778110(trmt12)
HHIP117769315(trmt12)
HSP118111962(trmt12)
LCF108872492(trmt12)
SDU111232645(trmt12)
SLAL111648183(trmt12)
XGL120785067(trmt12)
HCQ109524300(trmt12)
BPEC110159037(trmt12)
MALB109955755(trmt12)
BSPL114864222(trmt12)
OTW112255681(trmt12)
OMY110504438(trmt12)
OGO124010661(trmt12)
ONE115129153(trmt12)
SALP111967158(trmt12)
CCLU121585941(trmt12)
ELS105029873 114828743
SFM108923167(trmt12)
PKI111836516(trmt12)
AANG118223133(trmt12)
LOC102687083(trmt12)
PSPA121295211 121317916
ARUT117412022 117430509
LCM102350688(TRMT12)
CMK103177199(trmt12)
RTP109919454(trmt12)
BFO118431064
BBEL109484225
CIN100176207
SCLV120338208 120343551
SPU593691
APLC110982434
SKO100379063
CLEC106669520
HHAL106684077
NLU111056673
PHUPhum_PHUM229350
ZNE110836641
CSEC111870188
FCD110859278
DPXDAPPUDRAFT_308692
DMK116922965
EAF111697572
LSM121129251
ISCIscW_ISCW018117
DSV119432359
RSAN119405962
RMP119180822
DPTE113797541
DFR124490656
PTEP107453812
SDM118197138
CELCELE_F40F9.10(F40F9.10)
CBRCBG_19351
BMYBM_BM3972(Bm3972)
LOALOAG_02567
TSPTsp_06313
HROHELRODRAFT_67136
LGILOTGIDRAFT_128178
PCAN112560613
BGT106065493
GAE121381844
HRF124116850 124130760
HRJ124267683
CRG105332610
MYI110445483
PMAX117335742
MMER123536967
OBI106883232
OSN115212018
LAK106158204 106162049
NVE5520372
EPA110238807
ATEN116297840
ADF107333786
AMIL114977313
PDAM113683052
SPIS111341651
HMG100209695
TADTRIADDRAFT_18638
QSU111995520 112014999
SMOSELMODRAFT_105763 SELMODRAFT_153963
VCNVOLCADRAFT_121064
OLUOSTLU_34943
SCEYML005W(TRM12)
SEUBDI49_4085
SPAOSPAR_M01220
AGOAGOS_ADL199W
LTHKLTH0G10780g
VPOKpol_1003p49
ZROZYRO0A02266g
CGRCAGL0M06809g
NCSNCAS_0G03740(NCAS0G03740)
NDINDAI_0G00730(NDAI0G00730)
TPFTPHA_0G00810(TPHA0G00810)
TBLTBLA_0B06040(TBLA0B06040)
TDLTDEL_0G02320(TDEL0G02320)
TGBHG536_0A02160
KAFKAFR_0H03230(KAFR0H03230)
KNGKNAG_0H01920(KNAG0H01920)
ZMKHG535_0D01880
PPAPAS_chr2-2_0212
DHADEHA2A09988g
PICPICST_40540
PGUPGUG_01997
SPAASPAPADRAFT_62286
LELLELG_02782
CALCAALFM_C305890WA(TRM12)
CTPCTRG_02449
COTCORT_0C02050
CDUCD36_85820
CTENCANTEDRAFT_126976
YLIYALI0D01485g
CLUCLUG_04764
CLUSA9F13_11g00297
CAURCJI97_002286
SLBAWJ20_4217(TRM12)
PKZC5L36_0E01580
NCRNCU10483
NTENEUTE1DRAFT14617(NEUTE1DRAFT_14617)
SMPSMAC_08863
TTTTHITE_50004
MTMMYCTH_2311624
CTHRCTHT_0001960
MGRMGG_12102
PPEIPpBr36_04393
SSCKSPSK_00267
FGRFGSG_01465
FPUFPSE_08487
FVRFVEG_09544
FOXFOXG_11249
NHENECHADRAFT_30345
TRETRIREDRAFT_62780
TRRM419DRAFT_79027
MAWMAC_00071
MAJMAA_01780
CMTCCM_05153
PLJVFPFJ_07124
VALVDBG_01523
VDAVDAG_02033
CFJCFIO01_07245
CHIGCH63R_10285
ELAUCREL1_10218
PFYPFICI_03564
SSLSS1G_02487
BFUBCIN_13g03740
MBEMBM_06394
PSCOLY89DRAFT_573823
GLZGLAREA_01555
ANIAN0130.2
AFMAFUA_5G11650
ACTACLA_014720
NFINFIA_075620
AORAO090120000283
ANGANI_1_1248164(An18g03090)
AFVAFLA_009501
ALUCAKAW2_31276A
ACHEACHE_41175A
APUUAPUU_12021A
TMFPMAA_030860
TRGTRUGW13939_05635
CIMCIMG_09168
CPWCPC735_015860
UREUREG_05216
PBLPAAG_02406
PBNPADG_05548
ABEARB_03105
TVETRV_05817
AJEHCAG_00838
BGHBDBG_01012
PNOSNOG_08634
PTEPTT_04003
BZECOCCADRAFT_8699
BSCCOCSADRAFT_32516
BORCOCMIDRAFT_21718
AALTCC77DRAFT_994087
ZTRMYCGRDRAFT_87449
PFJMYCFIDRAFT_178656
FFUCLAFUR5_10890
BCOMBAUCODRAFT_47065
TMLGSTUM_00010910001
SPOSPAC4G8.06c(trm12)
PGRPGTG_07536
MLRMELLADRAFT_110558
MBRMONBRDRAFT_28161
SREPTSG_03546
PYOPY17X_0503900
PCBPCHAS_050290
PBEPBANKA_0502800
PKNPKNH_0602900
PVXPVX_001790
PCYPCYB_061290
FCYFRACYDRAFT_162398
TPSTHAPSDRAFT_262512
EHXEMIHUDRAFT_236459 EMIHUDRAFT_449936
GTTGUITHDRAFT_71161
TBRTb10.70.4420
TBGTbgDal_X3900
TCR504867.110 510943.180
LMALMJF_03_0060
LIFLINJ_03_0050
LDOLDBPK_030050
LMILMXM_03_0060
LBZLBRM_03_0070
LPANLPMP_030050
BIAGMA17_09795
MJAMJ_1557
MFEMefer_1490
MVUMetvu_0461
MFSMFS40622_0731
MIFMetin_0073
MJHJH146_0117
MESGMLAUSG7_1020(taw)
MESAMLASG1_0757(taw)
MIGMetig_0821
MMPMMP0560
MMQMmarC5_1047
MMXMmarC6_0333
MMZMmarC7_1579
MMDGYY_03680
MMAKMMKA1_06070
MMAOMMOS7_06020
MMADMMJJ_04250
MAEMaeo_0636
MVNMevan_1437
MVOMvol_0432
MOKMetok_0862
METFCFE53_01555
MTHMTH_1200
MMGMTBMA_c15780
MWOMWSIV6_1593(taw2)
MTEEMTTB_02270
METCMTCT_1093
METEtca_01157
METZMETMT2_1202
METKFVF72_05620
MTHMFZP57_07635
METJFZP68_03040
MSTMsp_0297
METBAW729_05650
MRUmru_1976
MSIMsm_1036
MEBAbm4_1548
MMILsm9_2048
MEYETL18_09425
MOLYLM1_1413
MAROMarbSA_15170
METVK4897_06285
MELMetbo_0325
MEWMSWAN_2093
METHMBMB1_1722(taw2)
MFCBRM9_0347
MFIDSM1535_0950(taw2)
MCUBMCBB_2023(taw2)
MSUBBK009_07640
METNBK008_03295
METTCIT01_03870
METOCIT02_09255
MEMEHYG87_05150
MFVMfer_0394
MKAMK0940
AFUAF_1973
AFGAFULGI_00022330
APOArcpr_0697
AVEArcve_1804
FPLFerp_1396
GACGACE_0368
GAHGAH_01121
PFUPF0500
PFIPFC_01620
PHOPH0793(PH0793)
PABPAB1538
PYNPNA2_1434
PYAPYCH_06810
PYSPy04_0683
PYCTQ32_03440
TKOTK0071
TONTON_0748
TGATGAM_0679
TSITSIB_1665
TBATERMP_00791
THEGQS_01185
THATAM4_598
THMCL1_1935
TLTOCC_03207
THSTES1_0720
TNUBD01_0979
TEUTEU_10690
TGYX802_06975
THVADU37_CDS16880
TCHCHITON_1204
TPEPA0127_03555
TPIEA7C91_09880
TGGA3K92_04860
TCEA3L02_08300
TBSA3L01_04505
THHCDI07_04420
TSLA3L11_08610
TTDA3L14_06455
TPRFA3L09_04985
TRLA3L10_02070
TPAFA3L08_07405
THYA3L12_07840
TICFH039_08765
TCQTIRI35C_1159(taw)
THEMFPV09_00915
THEIK1720_00970
PPACPAP_02950
MBAMbar_A2406
MBYMSBRM_1684
MBWMSBRW_2341
MBARMSBR2_0721
MBAKMSBR3_0463
MACMA_2064
MMAMM_3150
MMAZMmTuc01_3248
MMJMSMAS_1533
MMACMSMAC_2878
MVCMSVAZ_1273
MEKMSKOL_1218
MLSMSLAZ_1120
METMMSMTP_2061
MEFMSWH1_1514
MEQMSWHS_1793
MSJMSSAC_2051
MSZMSSIH_1716
MSWMSSIT_1771
MTHRMSTHT_0440
MTHEMSTHC_0281
MHORMSHOH_1874
MFZAOB57_000010
MBUMbur_0655
MMETMCMEM_0806
MELOJ7W08_02195
MMHMmah_1140
MHAZBHR79_07840
MEVMetev_1614
MZHMzhil_1347
MPYMpsy_3091
MZIHWN40_06085
MHZMetho_1531
MTPMthe_0736
MCJMCON_1956
MHIMhar_0127
MHUMhun_0829
MRTJKHC33_09470
MLAMlab_0923
MEMMemar_1237
MBGBN140_0994
MEMAMMAB1_1318
MCHKMchiMG62_14330
MPIMpet_0984
MBNMboo_1668
MFOMetfor_1346
MPLMpal_1885
MPDMCP_0205
MEZMtc_2504
RCIRCIX1355
HALVNG_2475C
HSLOE_4468F(taw2)
HDLHALDL1_01855
HHBHhub_3706(taw2)
HAGSJT689_11435
HABOJRZ79_09720
HNOLT974_00355
HLULT972_14355
HANRLJ422_10000
HJEHacjB3_01325
HALHHTSR_2016
HHSRHSR6_2092
HSUHLASF_2096
HSFHLASA_2130
SALRFQU85_04360
HALREFA46_003655
HALXM0R89_05625
HAXZM0R88_05610
SALIL593_09850
SAIMK0C01_04640
HARAAArcS_0374(tyw2)
HMArrnAC2902
HHIHAH_0171
HHNHISP_00940
HABSG26_08795
HTABVU17_00815
HALJG9465_03850
HSINKDQ40_13150
NPHNP_1360A(taw2)
NMONmlp_1176(taw2)
NHOHWV23_09385
NSALHWV07_12090
HUTHuta_1011
HTIHTIA_0874
HALAHrd1104_02660
HMUHmuk_1200
HALZE5139_09175
HALLLC1Hm_0413(tyw2)
HAZPGBQ70_09170
HALIBV210_07160
HSNDV733_09910
HDSHSR122_1806(trm5)
HRRHZS55_01290
HPELHZS54_07845
HLTI7X12_11445
HARCHARCEL1_02375
HTUHtur_0202
HDABB347_15275
HSALJMJ58_08250
HLOJ0X27_08645
HAKZJ0X25_07500
NMGNmag_2032(taw2)
HXAHalxa_1534
NATNJ7G_0351
NPENatpe_3771
NVRFEJ81_18065
NPLFGF80_16910
NAYHYG81_11080
HALYHYG82_12640
HJTDVR14_08920
NGENatgr_1484
HRUHalru_2763
NOUNatoc_0084
HLRHALLA_05055
HLCCHINAEXTREME03815
NAJB1756_07545
NAGAArcMg_0100
NANAArc1_0103
NBGDV706_05585
NASGCU68_05385
SAWLNGM29_03360
TACTa0926
TVOTVG1132799(TVG1132799)
PTOPTO0680
FACFACI_IFERC01G1166
FAIFAD_0193
CDIVCPM_1505
TARTALC_00197
MAXMMALV_01840
MERMMINT_17740
MEARMpt1_c12780(taw2)
MEUZKRP56_01035
MARCAR505_1645
ABIAboo_0011
ACFAciM339_0012
SSOSSO2223
SOLSsol_0041
SSOASULA_0039
SSOLSULB_0040
SSOFSULC_0039
SSHIJ5U23_01032
SCASSACC_20550
SAISaci_0030
SACNSacN8_00145
SACRSacRon12I_00145
SACSSUSAZ_00145
SISLS215_0039
SIAM1425_0039
SIMM1627_0039
SIDM164_0039
SIYYG5714_0039
SINYN1551_0039
SIILD85_0039
SIHSiH_0039
SIRSiRe_0039
SICSiL_0039
SULEGFS03_01730
SULLEWF20_12845
MEMJMJ1HA_0856
MJNMjAS7_1514
ACIHHS5_12190
CSTYKN1_05530
SAHSHS7_11120
CSUCSUB_C1650
LOKILokiarch_25700(taw2_1) Lokiarch_30240(taw2_2)
 » show all
Reference
1  [PMID:19717466]
  Authors
Umitsu M, Nishimasu H, Noma A, Suzuki T, Ishitani R, Nureki O
  Title
Structural basis of AdoMet-dependent aminocarboxypropyl transfer reaction catalyzed by tRNA-wybutosine synthesizing enzyme, TYW2.
  Journal
Proc Natl Acad Sci U S A 106:15616-21 (2009)
DOI:10.1073/pnas.0905270106
  Sequence
[mja:MJ_1557]
Reference
2  [PMID:22761755]
  Authors
Rodriguez V, Vasudevan S, Noma A, Carlson BA, Green JE, Suzuki T, Chandrasekharappa SC
  Title
Structure-function analysis of human TYW2 enzyme required for the biosynthesis of a highly modified Wybutosine (yW) base in phenylalanine-tRNA.
  Journal
PLoS One 7:e39297 (2012)
DOI:10.1371/journal.pone.0039297
Reference
3  [PMID:20382657]
  Authors
de Crecy-Lagard V, Brochier-Armanet C, Urbonavicius J, Fernandez B, Phillips G, Lyons B, Noma A, Alvarez S, Droogmans L, Armengaud J, Grosjean H
  Title
Biosynthesis of wyosine derivatives in tRNA: an ancient and highly diverse pathway in Archaea.
  Journal
Mol Biol Evol 27:2062-77 (2010)
DOI:10.1093/molbev/msq096
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.114
IUBMB Enzyme Nomenclature: 2.5.1.114
ExPASy - ENZYME nomenclature database: 2.5.1.114
BRENDA, the Enzyme Database: 2.5.1.114

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