KEGG   ENZYME: 2.5.1.149
Entry
EC 2.5.1.149                Enzyme                                 

Name
lycopene elongase/hydratase (flavuxanthin-forming);
crtEb (gene name);
dimethylallyl-diphosphate:all-trans-lycopene dimethylallyltransferase (hydrating, flavuxanthin-forming)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
prenyl-diphosphate:all-trans-lycopene prenyltransferase (hydrating, flavuxanthin-forming)
Reaction(IUBMB)
(1) prenyl diphosphate + all-trans-lycopene + acceptor + H2O = nonaflavuxanthin + reduced electron acceptor + diphosphate [RN:R12283];
(2) prenyl diphosphate + nonaflavuxanthin + acceptor + H2O = flavuxanthin + reduced electron acceptor + diphosphate [RN:R12284]
Reaction(KEGG)
R12283 R12284
Substrate
prenyl diphosphate [CPD:C00235];
all-trans-lycopene [CPD:C05432];
acceptor [CPD:C00028];
H2O [CPD:C00001];
nonaflavuxanthin [CPD:C22056]
Product
nonaflavuxanthin [CPD:C22056];
reduced electron acceptor;
diphosphate [CPD:C00013];
flavuxanthin [CPD:C22057]
Comment
The enzyme, characterized from the bacterium Corynebacterium glutamicum, is bifunctional. It catalyses the elongation of the C40 carotenoid all-trans-lycopene by attaching an isoprene unit at C-2, as well as the hydroxylation of the new isoprene unit. The enzyme acts at both ends of the substrate, forming the C50 carotenoid flavuxanthin via the C45 intermediate nonaflavuxanthin. cf. EC 2.5.1.150, lycopene elongase/hydratase (dihydrobisanhydrobacterioruberin-forming).
History
EC 2.5.1.149 created 2018
Orthology
K23155  lycopene elongase/hydratase (flavuxanthin-forming)
Genes
SCIA: HUG15_19465
CGL: Cgl0620(Cgl0620)
CGB: cg0717(crtEb)
CGU: WA5_0594(CrtEb)
CGT: cgR_0740
CGS: C624_03875(ubiA)
CGG: C629_03875(ubiA)
CGM: cgp_0717(crtEb)
CGJ: AR0_03700(ubiA)
CGQ: CGLAR1_03560(ubiA)
CGX: SB89_03315(ubiA)
CEF: CE0637(crtEb)
CHN: A605_03535(ubiA)
CCN: H924_00345(ubiA)
CMD: B841_05595(ubiA)
CFN: CFAL_10280(ubiA)
CVT: B843_10365(ubiA)
CUV: CUREI_00105(ubiA)
CHM: B842_00595(ubiA)
CMQ: B840_00375(ubiA)
CMV: CMUST_03130(ubiA)
CDX: CDES_03195(ubiA)
CSTA: CSTAT_10575(ubiA)
CCJZ: ccrud_03140(ubiA)
CFC: CFLV_10475(ubiA)
CRL: NCTC7448_00519(ubiA)
CSUR: N24_0758(ubiA)
CCYS: SAMEA4530656_2401(ubiA)
BFV: C628_03535(ubiA)
 » show all
Reference
1  [PMID:11432736]
  Authors
Krubasik P, Kobayashi M, Sandmann G
  Title
Expression and functional analysis of a gene cluster involved in the synthesis of decaprenoxanthin reveals the mechanisms for C50 carotenoid formation.
  Journal
Eur J Biochem 268:3702-8 (2001)
DOI:10.1046/j.1432-1327.2001.02275.x
  Sequence
[cgb:cg0717]
Reference
2  [PMID:22963379]
  Authors
Heider SA, Peters-Wendisch P, Wendisch VF
  Title
Carotenoid biosynthesis and overproduction in Corynebacterium glutamicum.
  Journal
BMC Microbiol 12:198 (2012)
DOI:10.1186/1471-2180-12-198
  Sequence
[cgb:cg0717]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.149
IUBMB Enzyme Nomenclature: 2.5.1.149
ExPASy - ENZYME nomenclature database: 2.5.1.149
BRENDA, the Enzyme Database: 2.5.1.149

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