KEGG   ENZYME: 2.5.1.151Help
Entry
EC 2.5.1.151                Enzyme                                 

Name
alkylcobalamin dealkylase;
MMACHC (gene name)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
alkylcobalamin:glutathione S-alkyltransferase
Reaction(IUBMB)
an alkylcobalamin + [alkylcobalamin reductase] + glutathione = cob(I)alamin-[alkylcobalamin reductase] + an S-alkylglutathione
Substrate
alkylcobalamin;
[alkylcobalamin reductase];
glutathione [CPD:C00051]
Product
cob(I)alamin-[alkylcobalamin reductase];
S-alkylglutathione
Comment
This mammalian enzyme, which is cytosolic, can bind internalized alkylcobalamins and process them to cob(I)alamin using the thiolate of glutathione for nucleophilic displacement. The product remains bound to the protein, and, following its oxidation to cob(II)alamin, is transferred by the enzyme, together with its interacting partner MMADHC, directly to downstream enzymes involved in adenosylcobalamin and methylcobalamin biosynthesis. In addition to its dealkylase function, the enzyme also catalyse an entirely different decyanase reaction with cyanocobalamin [cf. EC 1.16.1.6, cyanocobalamin reductase (cyanide-eliminating)].
History
EC 2.5.1.151 created 2018
Orthology
K14618  cyanocobalamin reductase (cyanide-eliminating) / alkylcobalamin dealkylase
Genes
HSA: 25974(MMACHC)
PTR: 469180(MMACHC)
PPS: 100968335(MMACHC)
GGO: 101152807(MMACHC)
PON: 100455100(MMACHC)
NLE: 100580574(MMACHC)
MCC: 706247(MMACHC)
MCF: 102121304(MMACHC)
CSAB: 103224912(MMACHC)
RRO: 104662137(MMACHC)
RBB: 108535651(MMACHC)
CJC: 100399324(MMACHC)
SBQ: 101027482(MMACHC)
MMU: 67096(Mmachc)
MCAL: 110293555(Mmachc)
MPAH: 110323588(Mmachc)
RNO: 313520(Mmachc)
MUN: 110546536(Mmachc)
CGE: 100751045(Mmachc)
NGI: 103737785(Mmachc)
HGL: 101709397(Mmachc)
OCU: 100347413(MMACHC)
TUP: 102472726(MMACHC)
CFA: 482514(MMACHC)
VVP: 112914783(MMACHC)
AML: 100466484(MMACHC)
UMR: 103670776(MMACHC)
UAH: 113254743(MMACHC)
ORO: 101379031(MMACHC)
ELK: 111142962
FCA: 101090955(MMACHC)
PTG: 102954225(MMACHC)
PPAD: 109266970(MMACHC)
AJU: 106970243(MMACHC)
BTA: 513433(MMACHC)
BOM: 102277584(MMACHC)
BIU: 109556357(MMACHC)
BBUB: 102401472(MMACHC)
CHX: 102183931(MMACHC)
OAS: 101120707(MMACHC)
SSC: 100513159(MMACHC)
CFR: 102509232(MMACHC)
CDK: 105090870(MMACHC)
BACU: 103014111(MMACHC)
LVE: 103075700(MMACHC)
OOR: 101274733(MMACHC)
DLE: 111175748(MMACHC)
PCAD: 102979216(MMACHC)
ECB: 100065336(MMACHC)
EPZ: 103551877(MMACHC)
EAI: 106826139(MMACHC)
MYB: 102260677(MMACHC)
MYD: 102764382(MMACHC)
MNA: 107526768(MMACHC)
HAI: 109375517(MMACHC)
DRO: 112314606(MMACHC)
PALE: 102888849(MMACHC)
RAY: 107506891(MMACHC)
MJV: 108385733(MMACHC)
LAV: 100673499(MMACHC)
TMU: 101353030
MDO: 100024700(MMACHC)
SHR: 100914568(MMACHC)
PCW: 110209280(MMACHC)
OAA: 100077154(MMACHC)
GGA: 424597(MMACHC)
MGP: 100547376(MMACHC)
CJO: 107317442(MMACHC)
NMEL: 110402950(MMACHC)
APLA: 101804029(MMACHC)
ACYG: 106033094(MMACHC)
TGU: 105759460(MMACHC)
LSR: 110469630(MMACHC)
SCAN: 103815175(MMACHC)
GFR: 102038500(MMACHC)
FAB: 101815080(MMACHC)
PHI: 102110636(MMACHC)
PMAJ: 107208008(MMACHC)
CCAE: 111933112(MMACHC)
CCW: 104695241(MMACHC)
ETL: 114062118(MMACHC)
FPG: 101914760(MMACHC)
FCH: 102058033(MMACHC)
CLV: 102088492(MMACHC)
EGZ: 104126262(MMACHC)
NNI: 104015417(MMACHC)
ACUN: 113482722(MMACHC)
PADL: 103919135(MMACHC)
AAM: 106485930(MMACHC)
ASN: 102383499(MMACHC)
AMJ: 102560477(MMACHC)
PSS: 102459461(MMACHC)
CMY: 102936214(MMACHC)
CPIC: 101952441(MMACHC)
ACS: 100552851(mmachc)
PVT: 110073759(MMACHC)
PBI: 103063544(MMACHC)
PMUR: 107283422(MMACHC)
TSR: 106548837(MMACHC)
PMUA: 114599088(MMACHC)
GJA: 107107920(MMACHC)
XLA: 100127331(mmachc.L)
XTR: 549685(mmachc)
NPR: 108800338(MMACHC)
DRE: 555267(mmachc)
SRX: 107757670(mmachc)
SANH: 107653140(mmachc)
SGH: 107565041(mmachc)
CCAR: 109051604(mmachc)
IPU: 100528283(mmachc)
PHYP: 113546876(mmachc)
AMEX: 103032715(mmachc)
EEE: 113591323(mmachc)
TRU: 101073402(mmachc)
LCO: 104926001(mmachc)
NCC: 104967760(mmachc)
MZE: 101475866(mmachc)
ONL: 100705297(mmachc)
OLA: 101155593(mmachc)
XMA: 102223263(mmachc)
XCO: 114150362(mmachc)
PRET: 103463749(mmachc)
CVG: 107096364(mmachc)
NFU: 107396398(mmachc)
KMR: 108230696(mmachc)
ALIM: 106533393(mmachc)
AOCE: 111564984(mmachc)
CSEM: 103376733(mmachc)
POV: 109625330(mmachc)
LCF: 108879008(mmachc)
SDU: 111231994(mmachc)
SLAL: 111653738(mmachc)
HCQ: 109532243(mmachc)
BPEC: 110154834(mmachc)
MALB: 109974876(mmachc)
SASA: 100196847(mmachc)
OTW: 112251067(mmachc)
SALP: 111975270(mmachc)
ELS: 105011679(mmachc)
SFM: 108923522(mmachc)
PKI: 111834817(mmachc)
LCM: 102353117(MMACHC)
CMK: 103182780(mmachc)
CIN: 100181296
SPU: 575015
APLC: 110989375
SKO: 100368262
FCD: 110861518
DPTE: 113791070
CEL: CELE_ZK546.17(cblc-1)
CBR: CBG02282(Cbr-mdt-4)
TSP: Tsp_00699
PCAN: 112574267
CRG: 105331412
MYI: 110443657
LAK: 106178486
SHX: MS3_11205
EPA: 110237339
ADF: 107347741
AMIL: 114956024
PDAM: 113667854
SPIS: 111334144
DGT: 114526387
HMG: 100212172
AQU: 100641773
 » show all
Taxonomy
Reference
1  [PMID:19447654]
  Authors
Hannibal L, Kim J, Brasch NE, Wang S, Rosenblatt DS, Banerjee R, Jacobsen DW
  Title
Processing of alkylcobalamins in mammalian cells: A role for the MMACHC (cblC) gene product.
  Journal
Mol Genet Metab 97:260-6 (2009)
DOI:10.1016/j.ymgme.2009.04.005
  Sequence
[hsa:25974]
Reference
2  [PMID:19801555]
  Authors
Kim J, Hannibal L, Gherasim C, Jacobsen DW, Banerjee R
  Title
A human vitamin B12 trafficking protein uses glutathione transferase activity for processing alkylcobalamins.
  Journal
J Biol Chem 284:33418-24 (2009)
DOI:10.1074/jbc.M109.057877
  Sequence
[hsa:25974]
Reference
3  [PMID:21697092]
  Authors
Koutmos M, Gherasim C, Smith JL, Banerjee R
  Title
Structural basis of multifunctionality in a vitamin B12-processing enzyme.
  Journal
J Biol Chem 286:29780-7 (2011)
DOI:10.1074/jbc.M111.261370
  Sequence
[hsa:25974]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.151
IUBMB Enzyme Nomenclature: 2.5.1.151
ExPASy - ENZYME nomenclature database: 2.5.1.151
BRENDA, the Enzyme Database: 2.5.1.151

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