KEGG   ENZYME: 2.5.1.151
Entry
EC 2.5.1.151                Enzyme                                 
Name
alkylcobalamin dealkylase;
MMACHC (gene name);
alkylcobalamin:glutathione S-alkyltransferase;
alkylcobalamin reductase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
methylcobalamin:glutathione S-methyltransferase
Reaction(IUBMB)
(1) methylcob(III)alamin + [alkylcobalamin dealkylase] + glutathione = cob(I)alamin-[alkylcobalamin dealkylase] + an S-methyl glutathione;
(2) adenosylcob(III)alamin + [alkylcobalamin dealkylase] + glutathione = cob(I)alamin-[alkylcobalamin dealkylase] + S-adenosyl glutathione
Substrate
methylcob(III)alamin [CPD:C06453];
[alkylcobalamin dealkylase];
glutathione [CPD:C00051];
adenosylcob(III)alamin [CPD:C00194]
Product
cob(I)alamin-[alkylcobalamin dealkylase];
S-methyl glutathione [CPD:C11347];
S-adenosyl glutathione
Comment
This mammalian enzyme, which is cytosolic, can bind internalized methylcob(III)alamin and adenosylcob(III)alamin and process them to cob(I)alamin using the thiolate of glutathione for nucleophilic displacement. The product remains bound to the protein, and, following its oxidation to cob(II)alamin, is transferred by the enzyme, together with its interacting partner MMADHC, directly to downstream enzymes involved in adenosylcob(III)alamin and methylcob(III)alamin biosynthesis. In addition to its dealkylase function, the enzyme also catalyse an entirely different decyanase reaction with cyanocob(III)alamin (cf. EC 1.16.1.6, cyanocobalamin reductase).
History
EC 2.5.1.151 created 2018, modified 2021
Orthology
K14618  cyanocobalamin reductase (cyanide-eliminating) / alkylcobalamin dealkylase
Genes
HSA25974(MMACHC)
PTR469180(MMACHC)
PPS100968335(MMACHC)
GGO101152807(MMACHC)
PON100455100(MMACHC)
NLE100580574(MMACHC)
MCC706247(MMACHC)
MCF102121304(MMACHC)
CSAB103224912(MMACHC)
CATY105594589(MMACHC)
PANU100999738(MMACHC) 101002954
TGE112614238(MMACHC)
RRO104662137(MMACHC)
RBB108535651(MMACHC)
TFN117092676(MMACHC)
PTEH111548761(MMACHC)
CJC100399324(MMACHC)
SBQ101027482(MMACHC)
CSYR103259911(MMACHC)
MMUR105877469(MMACHC)
OGA100951966(MMACHC)
MMU67096(Mmachc)
MCAL110293555(Mmachc)
MPAH110323588(Mmachc)
RNO313520(Mmachc)
MCOC116096478(Mmachc)
MUN110546536(Mmachc)
CGE100751045(Mmachc)
PLEU114707071(Mmachc)
NGI103737785(Mmachc)
HGL101709397(Mmachc)
CPOC100735601(Mmachc)
CCAN109686603 109701349
DORD105986053(Mmachc)
DSP122108018(Mmachc)
NCAR124985468
OCU100347413(MMACHC)
OPI101526373(MMACHC)
TUP102472726(MMACHC)
CFA482514(MMACHC)
VVP112914783(MMACHC)
VLG121481537(MMACHC)
AML100466484(MMACHC)
UMR103670776(MMACHC)
UAH113254743(MMACHC)
UAR123793762(MMACHC)
ELK111142962
LLV125096845
MPUF101689342(MMACHC)
ORO101379031(MMACHC)
EJU114224136(MMACHC)
ZCA113914085(MMACHC)
MLX118019481(MMACHC)
FCA101090955(MMACHC)
PYU121027034(MMACHC)
PBG122480425(MMACHC)
PTG102954225(MMACHC)
PPAD109266970(MMACHC)
AJU106970243(MMACHC)
HHV120247062(MMACHC)
BTA513433(MMACHC)
BOM102277584(MMACHC)
BIU109556357(MMACHC)
BBUB102401472(MMACHC)
CHX102183931(MMACHC)
OAS101120707(MMACHC)
ODA120856239(MMACHC)
CCAD122436862 122437053(MMACHC)
SSC100513159(MMACHC)
CFR102509232(MMACHC)
CBAI105065473(MMACHC)
CDK105090870(MMACHC)
VPC102531671(MMACHC)
BACU103014111(MMACHC)
LVE103075700(MMACHC)
OOR101274733(MMACHC)
DLE111175748(MMACHC)
PCAD102979216(MMACHC)
PSIU116741286(MMACHC)
ECB100065336(MMACHC)
EPZ103551877(MMACHC)
EAI106826139(MMACHC)
MYB102260677(MMACHC)
MYD102764382(MMACHC)
MMYO118677675(MMACHC)
MLF102431183(MMACHC)
MNA107526768(MMACHC)
PKL118724531(MMACHC)
HAI109375517(MMACHC)
DRO112314606(MMACHC)
SHON118982442(MMACHC)
AJM119052514(MMACHC)
PDIC114496741(MMACHC)
PHAS123831850(MMACHC)
MMF118626637(MMACHC)
RFQ117026819(MMACHC)
PALE102888849(MMACHC)
PGIG120620111(MMACHC)
PVP105297130(MMACHC)
RAY107506891(MMACHC)
MJV108385733(MMACHC)
TOD119236402(MMACHC)
SARA101549331(MMACHC)
LAV100673499(MMACHC)
TMU101353030
DNM101437454(MMACHC)
MDO100024700(MMACHC)
GAS123245274(MMACHC)
SHR100914568(MMACHC)
PCW110209280(MMACHC)
OAA100077154(MMACHC)
GGA424597(MMACHC)
PCOC116234140(MMACHC)
MGP100547376(MMACHC)
CJO107317442(MMACHC)
NMEL110402950(MMACHC)
APLA101804029(MMACHC)
ACYG106033094(MMACHC)
AFUL116492152(MMACHC)
TGU105759460(MMACHC)
LSR110469630(MMACHC)
SCAN103815175(MMACHC)
PMOA120503263(MMACHC)
OTC121345068(MMACHC)
PRUF121362233(MMACHC)
GFR102038500(MMACHC)
FAB101815080(MMACHC)
PHI102110636(MMACHC)
PMAJ107208008(MMACHC)
CCAE111933112(MMACHC)
CCW104695241(MMACHC)
ETL114062118(MMACHC)
ZAB102065882(MMACHC)
FPG101914760(MMACHC)
FCH102058033(MMACHC)
CLV102088492(MMACHC)
EGZ104126262(MMACHC)
NNI104015417(MMACHC)
ACUN113482722(MMACHC)
TALA104368702(MMACHC)
PADL103919135(MMACHC)
ACHC115349655(MMACHC)
AAM106485930(MMACHC)
AROW112975991(MMACHC)
NPD112942572(MMACHC)
DNE112985541(MMACHC)
ASN102383499(MMACHC)
AMJ102560477(MMACHC)
CPOO109307416(MMACHC)
GGN109286722(MMACHC)
PSS102459461(MMACHC)
CMY102936214(MMACHC)
CPIC101952441(MMACHC)
TST117881958(MMACHC)
CABI116838969(MMACHC)
MRV120370022(MMACHC)
ACS100552851(mmachc)
PVT110073759(MMACHC)
SUND121928329(MMACHC)
PBI103063544(MMACHC)
PMUR107283422(MMACHC)
TSR106548837(MMACHC)
PGUT117664138(MMACHC)
VKO123029289(MMACHC)
PMUA114599088(MMACHC)
ZVI118088724(MMACHC)
GJA107107920(MMACHC)
XLA100127331(mmachc.L)
XTR549685(mmachc)
NPR108800338(MMACHC)
BBUF120979437(MMACHC)
BGAR122943119(MMACHC)
DRE555267(mmachc)
SRX107757670(mmachc)
SANH107653140(mmachc)
SGH107565041(mmachc)
CCAR109051604(mmachc)
CAUA113037680(mmachc)
IPU100528283(mmachc)
PHYP113546876(mmachc)
SMEO124377465(mmachc)
TFD113648086(mmachc)
AMEX103032715(mmachc)
EEE113591323(mmachc)
TRU101073402(mmachc)
TNGGSTEN00020526G001
LCO104926001(mmachc)
NCC104967760(mmachc)
CGOB115006728(mmachc)
ELY117253630(mmachc)
PLEP121941263(mmachc)
SLUC116063239(mmachc)
ECRA117950442(mmachc)
PFLV114562020(mmachc)
GAT120823682(mmachc)
PPUG119216598(mmachc)
MSAM119900990(mmachc)
CUD121511797(mmachc)
MZE101475866(mmachc)
ONL100705297(mmachc)
OAU116331220(mmachc)
OLA101155593(mmachc)
OML112150160(mmachc)
XMA102223263(mmachc)
XCO114150362(mmachc)
XHE116725181(mmachc)
PRET103463749(mmachc)
PFOR103137034(mmachc)
PLAI106961240(mmachc)
PMEI106920743(mmachc)
GAF122838399(mmachc)
CVG107096364(mmachc)
CTUL119799275(mmachc)
GMU124874019(mmachc)
NFU107396398(mmachc)
KMR108230696(mmachc)
ALIM106533393(mmachc)
NWH119407999(mmachc)
AOCE111564984(mmachc)
CSEM103376733(mmachc)
POV109625330(mmachc)
SSEN122780298(mmachc)
HHIP117760846(mmachc)
LCF108879008(mmachc)
SDU111231994(mmachc)
SLAL111653738(mmachc)
XGL120791223(mmachc)
HCQ109532243(mmachc)
BPEC110154834(mmachc)
MALB109974876(mmachc)
SASA100196847(mmachc)
OTW112251067(mmachc)
OMY110524489(mmachc)
OGO123996891(mmachc)
ONE115108479(mmachc)
SALP111975270(mmachc)
SNH120054506(mmachc)
ELS105011679(mmachc)
SFM108923522(mmachc)
PKI111834817(mmachc)
AANG118225447(mmachc)
LOC102684271(mmachc)
PSPA121326789(mmachc)
ARUT117416980(mmachc)
LCM102353117(MMACHC)
CMK103182780(mmachc)
BFO118431891
BBEL109476117
CIN100181296
SCLV120342038
SPU575015
APLC110989375
SKO100368262
FCD110861518
DPXDAPPUDRAFT_309806
DMK116919432
HAME121863227
PTRU123518301
HAZT108667502
EAF111706502
ISCIscW_ISCW000540
DSV119433119
RSAN119404023
RMP119180682
DPTE113791070
DFR124497394
CSCU111617610 111620357
SDM118183440
CELCELE_ZK546.17(cblc-1)
CBRCBG_28142
NAINECAME_11211
TSPTsp_00699
HROHELRODRAFT_62766
PCAN112574267
BGT106064335
GAE121373901 121375291
HRF124146274
HRJ124254934
CRG105331412
MYI110443657
PMAX117325773
OBI106878149 106878150 106878154 106880449
OSN115232349
LAK106178486
OVIT265_05642
NVE5510973
EPA110237339
ATEN116307974
ADF107347741
AMIL114956024
PDAM113667854
SPIS111334144
DGT114526387
XEN124442138
HMG100212172
TADTRIADDRAFT_29163
AQU100641773
AHF112754217
CSLCOCSUDRAFT_56585
MISMICPUN_57097
SREPTSG_04387
EHXEMIHUDRAFT_460327 EMIHUDRAFT_465565
VINAKJ08_2395
 » show all
Reference
1  [PMID:19447654]
  Authors
Hannibal L, Kim J, Brasch NE, Wang S, Rosenblatt DS, Banerjee R, Jacobsen DW
  Title
Processing of alkylcobalamins in mammalian cells: A role for the MMACHC (cblC) gene product.
  Journal
Mol Genet Metab 97:260-6 (2009)
DOI:10.1016/j.ymgme.2009.04.005
  Sequence
[hsa:25974]
Reference
2  [PMID:19801555]
  Authors
Kim J, Hannibal L, Gherasim C, Jacobsen DW, Banerjee R
  Title
A human vitamin B12 trafficking protein uses glutathione transferase activity for processing alkylcobalamins.
  Journal
J Biol Chem 284:33418-24 (2009)
DOI:10.1074/jbc.M109.057877
  Sequence
[hsa:25974]
Reference
3  [PMID:21697092]
  Authors
Koutmos M, Gherasim C, Smith JL, Banerjee R
  Title
Structural basis of multifunctionality in a vitamin B12-processing enzyme.
  Journal
J Biol Chem 286:29780-7 (2011)
DOI:10.1074/jbc.M111.261370
  Sequence
[hsa:25974]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.151
IUBMB Enzyme Nomenclature: 2.5.1.151
ExPASy - ENZYME nomenclature database: 2.5.1.151
BRENDA, the Enzyme Database: 2.5.1.151

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