KEGG   ENZYME: 2.5.1.153
Entry
EC 2.5.1.153                Enzyme                                 
Name
adenosine tuberculosinyltransferase;
Rv3378c (locus name)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
tuberculosinyl-diphosphate:adenosine tuberculosinyltransferase
Reaction(IUBMB)
tuberculosinyl diphosphate + adenosine = 1-tuberculosinyladenosine + diphosphate [RN:R12669]
Reaction(KEGG)
R12669
Substrate
tuberculosinyl diphosphate [CPD:C19836];
adenosine [CPD:C00212]
Product
1-tuberculosinyladenosine [CPD:C22319];
diphosphate [CPD:C00013]
Comment
The enzyme, characterized from the bacterial pathogen Mycobacterium tuberculosis, produces 1-tuberculosinyladenosine, an unusual terpene nucleoside that acts as a phagolysosome disruptor by neutralizing the pH, resulting in swelling of the lysosome and obliteration of its multilamellar structure.
History
EC 2.5.1.153 created 2011 as EC 3.1.7.8 and EC 3.1.7.9, transferred 2020 to EC 2.5.1.153
Orthology
K15911  adenosine tuberculosinyltransferase
Genes
MFBMFUL124B02_24610
AGEAA314_01782
MTURv3378c
MTVRVBD_3378c
MTCMT3488
MRAMRA_3418
MTFTBFG_13414
MTBTBMG_03429
MTKTBSG_03452
MTZTBXG_003406
MTGMRGA327_20850
MTIMRGA423_21280
MTURCFBS_3580
MTOMTCTRI2_3451
MTDUDA_3378c
MTJJ112_18180
MTUBMT7199_3426
MTUCJ113_23650
MTUEJ114_18115
MTXM943_17435
MTUHI917_23750
MTULTBHG_03316
MTUTHKBT1_3567
MTUUHKBT2_3574
MTQHKBS1_3577
MBOBQ2027_MB3412C
MBBBCG_3449c
MBTJTY_3449
MBMBCGMEX_3447c
MBKK60_035170
MAFMAF_33930
MMICRN08_3726
MCEMCAN_34041
MCQBN44_80039
MCVBN43_70034
MCXBN42_50039
MCZBN45_70020
MLJMLAC_34860
MSHJMSHI_06150
RUBGBA63_14800
RMARGBA65_08760
 » show all
Reference
1  [PMID:24516143]
  Authors
Layre E, Lee HJ, Young DC, Martinot AJ, Buter J, Minnaard AJ, Annand JW, Fortune SM, Snider BB, Matsunaga I, Rubin EJ, Alber T, Moody DB.
  Title
Molecular profiling of Mycobacterium tuberculosis identifies tuberculosinyl nucleoside products of the virulence-associated enzyme Rv3378c.
  Journal
Proc Natl Acad Sci U S A 111:2978-83 (2014)
DOI:10.1073/pnas.1315883111
  Sequence
[mtu:Rv3378c]
Reference
2  [PMID:25910243]
  Authors
Young DC, Layre E, Pan SJ, Tapley A, Adamson J, Seshadri C, Wu Z, Buter J, Minnaard AJ, Coscolla M, Gagneux S, Copin R, Ernst JD, Bishai WR, Snider BB, Moody DB.
  Title
In vivo biosynthesis of terpene nucleosides provides unique chemical markers of Mycobacterium tuberculosis infection.
  Journal
Chem Biol 22:516-526 (2015)
DOI:10.1016/j.chembiol.2015.03.015
  Sequence
[mtu:Rv3378c]
Reference
3  [PMID:31427817]
  Authors
Buter J, Cheng TY, Ghanem M, Grootemaat AE, Raman S, Feng X, Plantijn AR, Ennis T, Wang J, Cotton RN, Layre E, Ramnarine AK, Mayfield JA, Young DC, Jezek Martinot A, Siddiqi N, Wakabayashi S, Botella H, Calderon R, Murray M, Ehrt S, Snider BB, Reed MB, Oldfield E, Tan S, Rubin EJ, Behr MA, van der Wel NN, Minnaard AJ, Moody DB.
  Title
Mycobacterium tuberculosis releases an antacid that remodels phagosomes.
  Journal
Nat Chem Biol 15:889-899 (2019)
DOI:10.1038/s41589-019-0336-0
  Sequence
[mtu:Rv3378c]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.153
IUBMB Enzyme Nomenclature: 2.5.1.153
ExPASy - ENZYME nomenclature database: 2.5.1.153
BRENDA, the Enzyme Database: 2.5.1.153

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