KEGG   ENZYME: 2.5.1.77Help
Entry
EC 2.5.1.77                 Enzyme                                 

Name
7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase;
FO synthase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
5-amino-6-(D-ribitylamino)uracil:4-hydroxyphenylpyruvate, 4-methylphenol transferase
Reaction(IUBMB)
5-amino-6-(D-ribitylamino)uracil + 3-(4-hydroxyphenyl)pyruvate + 2 S-adenosyl-L-methionine + H2O = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 2 L-methionine + 2 5'-deoxyadenosine + oxalate + NH3 [RN:R09396]
Reaction(KEGG)
Substrate
5-amino-6-(D-ribitylamino)uracil [CPD:C04732];
3-(4-hydroxyphenyl)pyruvate [CPD:C01179];
S-adenosyl-L-methionine [CPD:C00019];
H2O [CPD:C00001]
Product
7,8-didemethyl-8-hydroxy-5-deazariboflavin [CPD:C19154];
L-methionine [CPD:C00073];
5'-deoxyadenosine [CPD:C05198];
oxalate [CPD:C00209];
NH3 [CPD:C00014]
Comment
Binds a [4Fe-4S] cluster. The cluster is coordinated by 3 cysteines and an exchangeable AdoMet molecule. The first stage of catalysis is reduction of the 2 AdoMet to produce 2 methionine and 2 5'-deoxyadenosin-5-yl radicals that extract a hydrogen from each of the substrates permitting the condensation of the two [1]. The overall reaction catalysed is the transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino-6-ribitylaminopyrimidine-2,4(1H,3H)-dione to form 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO). 7,8-Didemethyl-8-hydroxy-5-deazariboflavin is the chromophore of the hydride carrier coenzyme F420 [1].
History
EC 2.5.1.77 created 2010
Pathway
ec00680  Methane metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K11779  FO synthase
K11780  FO synthase subunit 1
K11781  FO synthase subunit 2
Genes
CPS: CPS_4048 CPS_4049
ALT: ambt_18390(cofG) ambt_18395
AAL: EP13_00575 EP13_00580
AAUS: EP12_00555
ASP: AOR13_3368 AOR13_3369
ASQ: AVL57_01940 AVL57_01945
AAW: AVL56_00750 AVL56_00755
ZAL: AZF00_12035(fbiC)
OSG: BST96_15205
MAGA: Mag101_11125(fbiC)
SDF: ACG33_00245(fbiC)
PSPW: BJG93_33610(fbiC)
EBA: p2A350
BRK: CWS35_20200(fbiC)
RPB: RPB_4423
OCA: OCAR_5007
OCG: OCA5_c29510(fbiC)
SNO: Snov_4230
HMC: HYPMC_3547(fbiC)
RBM: TEF_20090(fbiC)
PZU: PHZ_c2536
PSF: PSE_2792(fbiC) PSE_2793(fbiC)
THAS: C6Y53_09640(cofH) C6Y53_09645(cofG)
NPN: JI59_17370(fbiC)
NRE: BES08_02715(fbiC) BES08_26705(fbiC)
SPHO: C3E99_13050(fbiC)
SWI: Swit_0717
SPHM: G432_01225(fbiC)
SPHJ: BSL82_04895(fbiC) BSL82_08920(fbiC)
SPHD: HY78_17635(fbiC) HY78_23110(fbiC)
SYB: TZ53_17125(fbiC) TZ53_18465(fbiC)
SBD: ATN00_17480(fbiC)
SPMI: K663_16861(fbiC)
SPHB: EP837_03073(fbiC)
SPHT: K426_12255(fbiC) K426_23844(fbiC)
SHYD: CJD35_15490(fbiC)
SYA: A6768_05840(fbiC)
BLAS: BSY18_2471(cofG)
RDI: CMV14_13670(fbiC) CMV14_16710(cofG)
CMAN: A9D14_14800(fbiC)
BBEV: BBEV_1717(fbiC)
MTU: Rv1173(fbiC)
MTV: RVBD_1173
MTC: MT1210
MRA: MRA_1184(fbiC)
MTG: MRGA327_07395(fbiC)
MTUR: CFBS_1252(fbiC)
MTO: MTCTRI2_1205(fbiC)
MTD: UDA_1173(fbiC)
MTN: ERDMAN_1316(fbiC)
MTJ: J112_06345(fbiC)
MTUE: J114_06345(fbiC)
MTX: M943_06155(fbiC)
MTUL: TBHG_01157
MTUT: HKBT1_1250(fbiC)
MTUU: HKBT2_1256(fbiC)
MTQ: HKBS1_1254(fbiC)
MBO: BQ2027_MB1206(fbiC)
MBB: BCG_1236(fbiC)
MBT: JTY_1209(fbiC)
MBM: BCGMEX_1208(fbiC)
MBX: BCGT_1005
MBZ: LH58_06470(fbiC)
MAF: MAF_11920(fbiC)
MCE: MCAN_11841(fbiC)
MCQ: BN44_11312(fbiC)
MCV: BN43_30241(fbiC)
MCX: BN42_21039(fbiC)
MCZ: BN45_30234(fbiC)
MMIC: RN08_1318
MLE: ML1492(fbiC)
MLB: MLBr01492
MPA: MAP_2611c
MAO: MAP4_1207
MAVI: RC58_05980(fbiC)
MAVU: RE97_05975(fbiC)
MAV: MAV_1312
MAVD: NF84_05830(fbiC)
MAVR: LA63_05960(fbiC)
MAVA: LA64_05975(fbiC)
MIT: OCO_12230
MIA: OCU_12190
MIE: LG41_05910(fbiC)
MID: MIP_01953
MYO: OEM_12380
MCHI: AN480_07165(fbiC)
MMAL: CKJ54_05940(fbiC)
MUL: MUL_1020(fbiC)
MMC: Mmcs_4032
MKM: Mkms_4107
MJL: Mjls_4262
MMI: MMAR_4279(fbiC)
MMAE: MMARE11_40940(fbiC)
MMM: W7S_05970(fbiC)
MLI: MULP_04464(fbiC)
MKN: MKAN_07265(fbiC)
MKS: LG40_07195(fbiC)
MKI: LH54_07205(fbiC)
MYV: G155_22255(fbiC)
MYE: AB431_23285(fbiC)
MHAD: B586_07415(fbiC)
MYN: MyAD_20555(fbiC)
MDX: BTO20_08855(fbiC)
MSM: MSMEG_5126(fbiC)
MSG: MSMEI_4998(fbiC)
MSB: LJ00_25350(fbiC)
MSN: LI99_25355(fbiC)
MSH: LI98_25360(fbiC)
MVA: Mvan_4533
MGI: Mflv_2167
MNE: D174_20945(fbiC)
MGO: AFA91_14910(fbiC)
MPHL: MPHLCCUG_04074(mqnE)
MVQ: MYVA_4308(fbiC)
MLL: B1R94_06840(fbiC)
MAB: MAB_1319
MMV: MYCMA_0695(fbiC)
MABB: MASS_1319(fbiC)
MAY: LA62_06685(fbiC)
MABO: NF82_06650(fbiC)
MAZ: LA61_06580(fbiC)
MAK: LH56_16495(fbiC)
MYS: NF92_16975(fbiC)
MYC: NF90_16980(fbiC)
MIZ: BAB75_07105(fbiC)
MSTE: MSTE_01287
MSAO: MYCSP_05810(fbiC)
MJD: JDM601_1161(fbiC)
ASD: AS9A_3366(fbiC)
NFA: NFA_47520
NCY: NOCYR_4499(fbiC)
NBR: O3I_035915(fbiC)
NNO: NONO_c64550(fbiC)
NSL: BOX37_25530(fbiC)
NSR: NS506_01897(fbiC)
NTP: CRH09_33525(fbiC)
RER: RER_41830(fbiC)
REY: O5Y_19555(fbiC)
REB: XU06_19700(fbiC)
ROP: ROP_60060(fbiC)
REQ: REQ_14170
RPY: Y013_22830(fbiC)
RHB: NY08_5017
RAV: AAT18_08315(fbiC)
RFA: A3L23_03076(fbiC)
RHW: BFN03_02640(fbiC)
RHS: A3Q41_00251(fbiC)
RRZ: CS378_23345(fbiC)
RHU: A3Q40_02330(fbiC)
RQI: C1M55_20650(fbiC)
RHQ: IM25_12240(fbiC)
GBR: Gbro_3324
GPO: GPOL_c31220(fbiC1)
GOR: KTR9_3363
GOQ: ACH46_05885(fbiC)
GOC: CXX93_08415(fbiC)
GIT: C6V83_14745(fbiC)
TPR: Tpau_1148
TSM: ASU32_06460(fbiC)
SRT: Srot_0835
DIT: C3V38_15800(fbiC)
DIZ: CT688_09915(fbiC)
DPC: A6048_10140(fbiC)
DLU: A6035_09660(fbiC)
SCO: SCO4429(fbiC)
SMA: SAVERM_3794(cofG)
SGR: SGR_4235
SGB: WQO_14275(fbiC)
SCB: SCAB_52181(fbiC)
SCT: SCAT_2338(fbiC)
SFA: Sfla_3633
SBH: SBI_06333(fbiC)
SHY: SHJG_4710
SVE: SVEN_3084
SDV: BN159_5026(fbiC)
SALB: XNR_2222
SALS: SLNWT_4334
STRP: F750_3084
SFI: SFUL_2886
SCI: B446_16865(fbiC)
SRC: M271_20760(fbiC) M271_38995(fbiC)
SALU: DC74_3703
SALL: SAZ_19675(fbiC)
SLV: SLIV_16290(fbiC)
SGU: SGLAU_14900(fbiC)
SVT: SVTN_16080(fbiC)
STRE: GZL_05186
SCW: TU94_14370(fbiC)
SLD: T261_4807
SLC: SL103_02830(fbiC)
STRM: M444_15375(fbiC)
STRC: AA958_12800(fbiC)
SAMB: SAM23877_3304(fbiC)
SPRI: SPRI_4317
SCZ: ABE83_21500(fbiC)
SCX: AS200_26105(fbiC)
SRW: TUE45_04323(mqnE_1)
STRF: ASR50_19115(fbiC)
SLE: sle_40200(sle_40200)
SRN: A4G23_02305(mqnE_1)
SPAV: Spa2297_14305(fbiC)
STRT: A8713_13345(fbiC)
SCLF: BB341_11230(fbiC)
SGS: AVL59_44175(fbiC)
STSI: A4E84_17315(fbiC)
SNR: SNOUR_23810(fbiC)
SPLU: LK06_018940(fbiC)
STRD: NI25_22395(fbiC)
SNW: BBN63_19680(fbiC)
SAUO: BV401_26765(fbiC) BV401_35045(fbiC)
SSIA: A7J05_20325(fbiC)
SVU: B1H20_13635(fbiC)
SPUN: BFF78_24080(fbiC)
SGV: B1H19_17935(fbiC)
SLAU: SLA_3135
SALF: SMD44_03660(fbiC)
SALJ: SMD11_3959(fbiC)
SLX: SLAV_16495(mqnE2)
STRO: STRMOE7_16995(fbiC)
SFK: KY5_3501c
KSK: KSE_45370(fbiC)
KAB: B7C62_13575(fbiC)
KAU: B6264_08870(fbiC)
MTS: MTES_1880
MCW: A8L33_02330(fbiC)
MIH: BJP65_10345(fbiC)
LMOI: VV02_14120(fbiC)
ARS: ADJ73_02705(fbiC)
NDK: I601_0314(mqnE)
KFL: Kfla_6490
PSIM: KR76_06880(fbiC)
TFU: Tfu_2519
NDA: Ndas_3771
NAL: B005_0949(cofG)
NGV: CDO52_24260(fbiC)
TCU: Tcur_4075
SRO: Sros_1376
NOA: BKM31_38790(fbiC)
FAL: FRAAL1263
ACE: Acel_0769
NML: Namu_4262
GOB: Gobs_1483
BSD: BLASA_1295(fbiC)
MMAR: MODMU_1466(fbiC)
KRA: Krad_3632
SEN: SACE_6594
AMD: AMED_6904(fbiC)
AMN: RAM_35420(fbiC)
AMM: AMES_6798(fbiC)
AMZ: B737_6798(fbiC)
AOI: AORI_1815(fbiC)
AJA: AJAP_30405(fbiC)
AMQ: AMETH_5642(fbiC)
AMYC: CU254_27985(fbiC)
AMYB: BKN51_01275(fbiC)
PDX: Psed_5307
PSEA: WY02_11900(fbiC)
PSEE: FRP1_02325(fbiC)
PSEH: XF36_01695(fbiC)
PSEQ: AD006_09945(fbiC)
PECQ: AD017_17775(fbiC)
PHH: AFB00_15685(fbiC)
AMI: Amir_6472
APRE: CNX65_31760(fbiC)
SESP: BN6_77580(fbiC)
KAL: KALB_8047
KPHY: AOZ06_48880(fbiC)
LED: BBK82_18465(fbiC)
ACTI: UA75_18405
ACAD: UA74_17915
AHG: AHOG_15900(mqnE)
ACTA: C1701_18075(fbiC)
ALL: CRK57346
PMAD: BAY61_03415(fbiC)
SAQ: Sare_0888
MIL: ML5_1202
VMA: VAB18032_09475(fbiC)
AMS: AMIS_8560
ASE: ACPL_1050(fbiC)
ACTN: L083_1155(fbiC)
AFS: AFR_05660(fbiC)
ACTS: ACWT_0932
PLAB: C6361_20395(fbiC)
PLAT: C6W10_23365(fbiC)
CAI: Caci_8766
TBI: Tbis_0746
AEY: CDG81_19075(fbiC)
RXY: Rxyl_0975
AFO: Afer_0900
AYM: YM304_13120(fbiC)
SYY: SYNGTS_0250(cofH) SYNGTS_0642(cofG)
SYT: SYNGTI_0250(cofH) SYNGTI_0642(cofG)
SYS: SYNPCCN_0250(cofH) SYNPCCN_0642(cofG)
SYQ: SYNPCCP_0250(cofH) SYNPCCP_0642(cofG)
TEL: tll1515(cofG) tlr1597(cofG)
THN: NK55_04200(cofG) NK55_04740(cofH)
CYI: CBM981_0450(cofH) CBM981_1919(cofG)
HHG: XM38_009170(cofH) XM38_019080(cofG)
CYL: AA637_02275(cofH) AA637_08445(cofG)
ARP: NIES39_K03090(cofG) NIES39_L02990(cofH)
GVI: gll2100(cofG) glr0776(cofG)
GLJ: GKIL_0029(cofG) GKIL_2220(cofH)
ANB: ANA_C11401(cofH) ANA_C12049(thiH)
NSP: BMF81_01795(cofG) BMF81_01996(cofH)
CEO: ETSB_1201
TRO: trd_A0899(cofH) trd_A0900(cofG)
TTR: Tter_2623
MMP: MMP0056(cofH) MMP0057(cofH) MMP0876(cofG)
MAC: MA_1489 MA_1490 MA_1491(bioB)
MCJ: MCON_1058(cofG) MCON_3335(cofH)
MBG: BN140_0224(cofH1) BN140_0551(cofG) BN140_1481(cofH3)
MEMA: MMAB1_0286(cofH) MMAB1_0691(cofG) MMAB1_1902(cofH)
MPD: MCP_2456(cofH-1) MCP_2457(cofH-2) MCP_2458(cofG)
MEZ: Mtc_0996(cofG) Mtc_0997(cofH-1) Mtc_0998(cofH-2)
RCI: RCIX459(cofH-2) RCIX460(cofH-1) RCIX461(cofG)
MMG: MTBMA_c12170(cofH) MTBMA_c15760(cofG)
MWO: MWSIV6_1196(cofH) MWSIV6_1591(cofG)
METE: tca_00787(fbiC_1) tca_01155(fbiC_2)
MST: Msp_0296(cofG) Msp_0392(cofH)
MRU: mru_1266(cofH) mru_1974(cofG)
MEB: Abm4_0772(cofH) Abm4_1547(cofG)
MMIL: sm9_0991(cofH) sm9_2047(cofG)
MEYE: TL18_05430(cofH) TL18_09420(cofG)
METH: MBMB1_0707(cofH1) MBMB1_1031(cofH3) MBMB1_1721(cofG)
MFC: BRM9_0974(cofG) BRM9_1217(cofH1) BRM9_1488(cofH2)
MFI: DSM1535_0944(cofG) DSM1535_1898(cofH1) DSM1535_2180(cofH3)
MCUB: MCBB_0164(cofH_1) MCBB_0722(cofH_3) MCBB_2020(cofG)
MKA: MK0897(thiH_1) MK0898(thiH_2)
HSL: OE_3722F(cofG) OE_3723R(cofH)
HHB: Hhub_3263(cofG) Hhub_3265(cofH)
HALH: HTSR_1968(cofH) HTSR_1969(cofG)
HHSR: HSR6_2043(cofH) HSR6_2044(cofG)
HHI: HAH_0519(cofH) HAH_0520(cofG)
NPH: NP_2394A(cofH) NP_2398A(cofG)
NMO: Nmlp_2441(cofG) Nmlp_3320(cofH)
HWA: HQ_3125A(cofG) HQ_3127A(cofH)
HWC: Hqrw_3666(cofG) Hqrw_3668(cofH)
HVO: HVO_2198(cofH) HVO_2201(cofG)
HME: HFX_2254(thiH) HFX_2258(thiH)
NMG: Nmag_2633(cofG) Nmag_2637(cofH)
SALI: L593_00095(cofG) L593_13865
NID: NPIRD3C_1665(cofG) NPIRD3C_1666(cofH)
NIN: NADRNF5_1568(cofG) NADRNF5_1569(cofH)
NCT: NMSP_1083(cofG) NMSP_1084(cofH_2)
NGA: Ngar_c12340(cofG) Ngar_c12350(cofH1)
NVN: NVIE_011280(cofH1) NVIE_011290(cofG)
TAA: NMY3_02529(mqnE_1) NMY3_02530
NCV: NCAV_0879(cofG)
NDV: NDEV_0671(cofH) NDEV_0672(cofG)
 » show all
Taxonomy
Reference
1  [PMID:14593448]
  Authors
Graham DE, Xu H, White RH
  Title
Identification of the 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase required for coenzyme F(420) biosynthesis.
  Journal
Arch Microbiol 180:455-64 (2003)
DOI:10.1007/s00203-003-0614-8
  Sequence
Reference
2  [PMID:11948155]
  Authors
Choi KP, Kendrick N, Daniels L
  Title
Demonstration that fbiC is required by Mycobacterium bovis BCG for coenzyme F(420) and FO biosynthesis.
  Journal
J Bacteriol 184:2420-8 (2002)
DOI:10.1128/JB.184.9.2420-2428.2002
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.77
IUBMB Enzyme Nomenclature: 2.5.1.77
ExPASy - ENZYME nomenclature database: 2.5.1.77
BRENDA, the Enzyme Database: 2.5.1.77

DBGET integrated database retrieval system