KEGG ENZYME: 2.5.1.81

ENZYME: 2.5.1.81

Entry

EC 2.5.1.81                 Enzyme

Name

geranylfarnesyl diphosphate synthase;
FGPP synthase;
(all-E) geranylfarnesyl diphosphate synthase;
GFPS;
Fgs

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

geranylgeranyl-diphosphate:isopentenyl-diphosphate transtransferase (adding 1 isopentenyl unit)

Reaction(IUBMB)

geranylgeranyl diphosphate + isopentenyl diphosphate = (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate [RN:R07475]

Reaction(KEGG)

R07475

Substrate

geranylgeranyl diphosphate [CPD:C00353];
isopentenyl diphosphate [CPD:C00129]

Product

(2E,6E,10E,14E)-geranylfarnesyl diphosphate [CPD:C04217];
diphosphate [CPD:C00013]

Comment

The enzyme from Methanosarcina mazei is involved in biosynthesis of the polyprenyl side-chain of methanophenazine, an electron carrier utilized for methanogenesis. It prefers geranylgeranyl diphosphate and farnesyl diphosphate as allylic substrate [1]. The enzyme from Aeropyrum pernix prefers farnesyl diphosphate as allylic substrate. The enzyme is involved in the biosynthesis of C25-C25 membrane lipids [2].

History

EC 2.5.1.81 created 2010

Pathway

ec00900

Terpenoid backbone biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K28131

geranylfarnesyl diphosphate synthase

Genes

MBA:	Mbar_A0394

MBY:	MSBRM_0598

MBW:	MSBRW_0605

MBAR:

MSBR2_1781

MBAK:

MSBR3_2906

MAC:

MA_4150

MMA:

MM_0789

MMAZ:

MmTuc01_0810

MMJ:	MSMAS_0513

MMAC:

MSMAC_0532

MVC:	MSVAZ_2966

MEK:	MSKOL_2943

MLS:	MSLAZ_2737

METM:

MSMTP_0557

MEF:	MSWH1_0588

MEQ:	MSWHS_0604

MSJ:	MSSAC_3923

MSZ:	MSSIH_3452

MSW:	MSSIT_3525

MTHR:

MSTHT_2268

MTHE:

MSTHC_1012

MHOR:

MSHOH_0605

MFZ:	AOB57_004430

MHAB:

RSJ42_13195

METW:

AAY43_05490

MBU:	Mbur_1235(gds)

MMET:

MCMEM_1687

MELO:

J7W08_07385

MMH:

Mmah_1452

MHAZ:

BHR79_06420

MPOT:

BKM01_02680

MEV:	Metev_0230

MZH:	Mzhil_1487

MPY:

Mpsy_2497

MZI:	HWN40_09445

MMAV:

RE476_00255

MSEB:

RE474_09995

MHZ:	Metho_0831

MEHF:

MmiHf6_14470

MEES:

MmiEs2_12570

MTP:

Mthe_0351

MCJ:

MCON_2794

MHI:

Mhar_0721

» show all

Reference

1 [PMID:20097171]

Authors

Ogawa T, Yoshimura T, Hemmi H

Title

Geranylfarnesyl diphosphate synthase from Methanosarcina mazei: Different role, different evolution.

Journal

Biochem Biophys Res Commun 393:16-20 (2010)
DOI:10.1016/j.bbrc.2010.01.063

Sequence

[mma:MM_0789]

Reference

2 [PMID:10632701]

Authors

Tachibana A, Yano Y, Otani S, Nomura N, Sako Y, Taniguchi M

Title

Novel prenyltransferase gene encoding farnesylgeranyl diphosphate synthase from a hyperthermophilic archaeon, Aeropyrum pernix. Molecularevolution with alteration in product specificity.

Journal

Eur J Biochem 267:321-8 (2000)
DOI:10.1046/j.1432-1327.2000.00967.x

Sequence

[ag:BAA88983]

Reference

3 [PMID:8137956]

Authors

Tachibana A

Title

A novel prenyltransferase, farnesylgeranyl diphosphate synthase, from the haloalkaliphilic archaeon, Natronobacterium pharaonis.

Journal

FEBS Lett 341:291-4 (1994)
DOI:10.1016/0014-5793(94)80475-3

Sequence

[nph:NP_3696A]

Reference

4 [PMID:15548566]

Authors

Lee PC, Mijts BN, Petri R, Watts KT, Schmidt-Dannert C

Title

Alteration of product specificity of Aeropyrum pernix farnesylgeranyl diphosphate synthase (Fgs) by directed evolution.

Journal

Protein Eng Des Sel 17:771-7 (2004)
DOI:10.1093/protein/gzh089

Sequence

[ag:BAA88983]

Other DBs

ExplorEnz - The Enzyme Database:

2.5.1.81

IUBMB Enzyme Nomenclature:

2.5.1.81

ExPASy - ENZYME nomenclature database:

2.5.1.81

BRENDA, the Enzyme Database:

2.5.1.81

All links

Protein sequence (734)   
   UniProt (106)   
   SWISS-PROT (17)   
   RefSeq(pep) (611)   
DNA sequence (1097)   
   RefSeq(nuc) (1011)   
   GenBank (47)   
   EMBL (39)   
Protein domain (5)   
   InterPro (5)   
All databases (1836)   

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