Entry
Name
pseudaminic acid synthase;
PseI;
NeuB3
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
phosphoenolpyruvate:2,4-bis(acetylamino)-2,4,6-trideoxy-beta-L-altropyranose transferase (phosphate-hydrolysing, 2,7-acetylamino-transfering, 2-carboxy-2-oxoethyl-forming)
Reaction(IUBMB)
phosphoenolpyruvate + 2,4-bis(acetylamino)-2,4,6-trideoxy-beta-L-altropyranose + H2O = 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid + phosphate [RN:
R09841 ]
Reaction(KEGG)
Substrate
phosphoenolpyruvate [CPD:
C00074 ];
2,4-bis(acetylamino)-2,4,6-trideoxy-beta-L-altropyranose;
H2O [CPD:
C00001 ]
Product
5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid [CPD:
C20082 ];
phosphate [CPD:
C00009 ]
Comment
The enzyme requires a divalent metal ion, the highest activity values are observed in the presence of Mn2+ and Co2+ (10 mM).
History
EC 2.5.1.97 created 2011
Pathway
ec00520 Amino sugar and nucleotide sugar metabolism
ec00541 O-Antigen nucleotide sugar biosynthesis
Orthology
K15898 pseudaminic acid synthase
Genes
CDIZ : CEDIAZO_01288(pseI)
HPO : HMPREF4655_20420(pseI)
HPX : HMPREF0462_0233(pseI)
HCL : NCTC13205_00368(spsE_1)
HSH : NHP194022_15540(neuB)
HPUL : NCTC13154_00281(spsE)
CJV : MTVDSCj20_1313(pseI)
CFV : CFVI03293_1587(pseI)
CAMP : CFT03427_1513(pseI)
CLR : UPTC16701_0178(pseI)
CLM : UPTC16712_0176(pseI)
CAMR : CAQ16704_0169(pseI)
CHV : CHELV3228_0385(pseI)
CPIN : CPIN18020_0127(pseI)
CAMZ : CVIC12175_0323(pseI)
PPOR : JCM14722_05450(spsE)
CCOH : SAMEA4530647_1491(spsE)
OCW : OW730_17355(pseI) OW730_21380(pseI)
HSC : HVS_01475(pseI1) HVS_04485(pseI2)
PSEZ : HME7025_02498(pseI)
MSCH : N508_001046(pseI_1) N508_001414(pseI_2)
» show all
Taxonomy
Reference
Authors
Chou WK, Dick S, Wakarchuk WW, Tanner ME
Title
Identification and characterization of NeuB3 from Campylobacter jejuni as a pseudaminic acid synthase.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.97
ExPASy - ENZYME nomenclature database: 2.5.1.97