KEGG   ENZYME: 2.6.1.114Help
Entry
EC 2.6.1.114                Enzyme                                 

Name
8-demethyl-8-aminoriboflavin-5'-phosphate synthase;
rosB (gene name)
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
BRITE hierarchy
Sysname
L-glutamate:FMN aminotransferase (oxidizing, decarboxylating)
Reaction(IUBMB)
L-glutamate + FMN + O2 + H2O + 3 acceptor = 2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + 3 reduced acceptor (overall reaction) [RN:R11960];
(1a) FMN + O2 = 8-demethyl-8-formylriboflavin 5'-phosphate + H2O [RN:R11961];
(1b) 8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor = 8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor [RN:R11962];
(1c) L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + 2 acceptor = 2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + 2 reduced acceptor [RN:R11963]
Reaction(KEGG)
Substrate
L-glutamate [CPD:C00025];
FMN [CPD:C00061];
O2 [CPD:C00007];
H2O [CPD:C00001];
acceptor [CPD:C00028];
8-demethyl-8-formylriboflavin 5'-phosphate [CPD:C21803];
8-carboxy-8-demethylriboflavin 5'-phosphate [CPD:C21804]
Product
2-oxoglutarate [CPD:C00026];
8-amino-8-demethylriboflavin 5'-phosphate [CPD:C21805];
CO2 [CPD:C00011];
reduced acceptor [CPD:C00030];
8-demethyl-8-formylriboflavin 5'-phosphate [CPD:C21803];
H2O [CPD:C00001];
8-carboxy-8-demethylriboflavin 5'-phosphate [CPD:C21804]
Comment
The enzyme, characterized from the bacterium Streptomyces davawensis, has the activities of an oxidoreductase, a decarboxylase, and an aminotransferase. Its combined actions result in the replacement of a methyl substituent of one of the aromatic rings of FMN by an amino group, a step in the biosynthetic pathway of roseoflavin. The reaction requires thiamine for completion.
History
EC 2.6.1.114 created 2018
Pathway
ec00998  Biosynthesis of secondary metabolites - other antibiotics
Orthology
K22598  8-demethyl-8-aminoriboflavin-5'-phosphate synthase
Genes
SDV: BN159_7989
Taxonomy
Reference
1  [PMID:27062037]
  Authors
Schwarz J, Konjik V, Jankowitsch F, Sandhoff R, Mack M
  Title
Identification of the Key Enzyme of Roseoflavin Biosynthesis.
  Journal
Angew Chem Int Ed Engl 55:6103-6 (2016)
DOI:10.1002/anie.201600581
  Sequence
Reference
2  [PMID:27331868]
  Authors
Jhulki I, Chanani PK, Abdelwahed SH, Begley TP
  Title
A Remarkable Oxidative Cascade That Replaces the Riboflavin C8 Methyl with an Amino Group during Roseoflavin Biosynthesis.
  Journal
J Am Chem Soc 138:8324-7 (2016)
DOI:10.1021/jacs.6b02469
  Sequence
Reference
3  [PMID:27981706]
  Authors
Konjik V, Brunle S, Demmer U, Vanselow A, Sandhoff R, Ermler U, Mack M
  Title
The Crystal Structure of RosB: Insights into the Reaction Mechanism of the First  Member of a Family of Flavodoxin-like Enzymes.
  Journal
Angew Chem Int Ed Engl 56:1146-1151 (2017)
DOI:10.1002/anie.201610292
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.114
IUBMB Enzyme Nomenclature: 2.6.1.114
ExPASy - ENZYME nomenclature database: 2.6.1.114
BRENDA, the Enzyme Database: 2.6.1.114

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