KEGG   ENZYME: 2.6.1.119
Entry
EC 2.6.1.119                Enzyme                                 

Name
vanillin aminotransferase;
VAMT (gene name)
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
Sysname
L-alanine:vanillin aminotransferase
Reaction(IUBMB)
L-alanine + vanillin = pyruvate + vanillylamine [RN:R12622]
Reaction(KEGG)
R12622
Substrate
L-alanine [CPD:C00041];
vanillin [CPD:C00755]
Product
pyruvate [CPD:C00022];
vanillylamine [CPD:C16666]
Comment
The enzyme participates in the biosynthesis of capsaicinoids in pungent cultivars of Capsicum sp. In vivo it has only been assayed in the reverse direction, where the preferred amino group acceptors were found to be pyruvate and oxaloacetate.
History
EC 2.6.1.119 created 2020
Pathway
ec00996  Biosynthesis of various alkaloids
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K24276  vanillin aminotransferase
Genes
SLY: 101258391
SPEN: 107027278
SOT: 102597871
CANN: 107854420(pAMT)
NAU: 109222880
Reference
1
  Authors
Curry, J., Aluru, M., Mendoza, M., Nevarez, J., Melendrez, M. and O'Connell, M.A.
  Title
Transcripts for possible capsaicinoid biosynthetic genes are differentially accumulated in pungent and non-pungent Capsicum spp.
  Journal
Plant Sci 148:47-57 (1999)
Reference
2  [PMID:17999078]
  Authors
del Rosario Abraham-Juarez M, del Carmen Rocha-Granados M, Lopez MG, Rivera-Bustamante RF, Ochoa-Alejo N
  Title
Virus-induced silencing of Comt, pAmt and Kas genes results in a reduction of capsaicinoid accumulation in chili pepper fruits.
  Journal
Planta 227:681-95 (2008)
DOI:10.1007/s00425-007-0651-7
Reference
3  [PMID:19473323]
  Authors
Lang Y, Kisaka H, Sugiyama R, Nomura K, Morita A, Watanabe T, Tanaka Y, Yazawa S, Miwa T.
  Title
Functional loss of pAMT results in biosynthesis of capsinoids, capsaicinoid analogs, in Capsicum annuum cv. CH-19 Sweet.
  Journal
Plant J 59:953-61 (2009)
DOI:10.1111/j.1365-313X.2009.03921.x
Reference
4  [PMID:22921003]
  Authors
Gururaj HB, Padma MN, Giridhar P, Ravishankar GA
  Title
Functional validation of Capsicum frutescens aminotransferase gene involved in vanillylamine biosynthesis using Agrobacterium mediated genetic transformation studies in Nicotiana tabacum and Capsicum frutescens calli cultures.
  Journal
Plant Sci 195:96-105 (2012)
DOI:10.1016/j.plantsci.2012.06.014
  Sequence
[cann:107854420]
Reference
5  [PMID:24712445]
  Authors
Weber N, Ismail A, Gorwa-Grauslund M, Carlquist M
  Title
Biocatalytic potential of vanillin aminotransferase from Capsicum chinense.
  Journal
BMC Biotechnol 14:25 (2014)
DOI:10.1186/1472-6750-14-25
  Sequence
[cann:107854420]
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.119
IUBMB Enzyme Nomenclature: 2.6.1.119
ExPASy - ENZYME nomenclature database: 2.6.1.119
BRENDA, the Enzyme Database: 2.6.1.119

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