| Entry |
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| Name |
neomycin C transaminase;
neoN (gene name)
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| Class |
Transferases;
Transferring nitrogenous groups;
Transaminases
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| Sysname |
2-oxoglutarate:neomycin C aminotransferase
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| Reaction(IUBMB) |
neomycin C + 2-oxoglutarate = 6'''-deamino-6'''-oxoneomycin C + L-glutamate [RN: R08903]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
6'''-deamino-6'''-oxoneomycin C [CPD: C17589];
L-glutamate [CPD: C00025]
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| Comment |
The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathway of aminoglycoside antibiotics of the neomycin family. Works in combination with EC 1.1.3.44, 6'''-hydroxyneomycin C oxidase, to replace the 6'''-hydroxy group of 6'''-deamino-6'''-hydroxyneomycin C with an amino group.
The enzyme, characterized from the bacterium Streptomyces fradiae, can also catalyse EC 2.6.1.93, neamine transaminase.
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| History |
EC 2.6.1.95 created 2012
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| Pathway |
| ec00524 | Neomycin, kanamycin and gentamicin biosynthesis |
| ec01110 | Biosynthesis of secondary metabolites |
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| Orthology |
| K20567 | neamine transaminase / neomycin C transaminase |
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| Genes |
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| Reference |
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| Authors |
Huang F, Spiteller D, Koorbanally NA, Li Y, Llewellyn NM, Spencer JB |
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| Title |
Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin. |
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| Journal |
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| Reference |
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| Authors |
Clausnitzer D, Piepersberg W, Wehmeier UF |
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| Title |
The oxidoreductases LivQ and NeoQ are responsible for the different 6'-modifications in the aminoglycosides lividomycin and neomycin. |
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| Journal |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 2.6.1.95 |
| ExPASy - ENZYME nomenclature database: | 2.6.1.95 |
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