KEGG   ENZYME: 2.6.1.95
Entry
EC 2.6.1.95                 Enzyme                                 
Name
neomycin C transaminase;
neoN (gene name)
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
Sysname
2-oxoglutarate:neomycin C aminotransferase
Reaction(IUBMB)
neomycin C + 2-oxoglutarate = 6'''-deamino-6'''-oxoneomycin C + L-glutamate [RN:R08903]
Reaction(KEGG)
R08903
Substrate
neomycin C [CPD:C15652];
2-oxoglutarate [CPD:C00026]
Product
6'''-deamino-6'''-oxoneomycin C [CPD:C17589];
L-glutamate [CPD:C00025]
Comment
The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathway of aminoglycoside antibiotics of the neomycin family. Works in combination with EC 1.1.3.44, 6'''-hydroxyneomycin C oxidase, to replace the 6'''-hydroxy group of 6'''-deamino-6'''-hydroxyneomycin C with an amino group.
The enzyme, characterized from the bacterium Streptomyces fradiae, can also catalyse EC 2.6.1.93, neamine transaminase.
History
EC 2.6.1.95 created 2012
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K20567  neamine transaminase / neomycin C transaminase
Genes
SRN: A4G23_05117(neoN)
SFB: CP974_27310
Reference
1  [PMID:17206729]
  Authors
Huang F, Spiteller D, Koorbanally NA, Li Y, Llewellyn NM, Spencer JB
  Title
Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin.
  Journal
Chembiochem 8:283-8 (2007)
DOI:10.1002/cbic.200600371
Reference
2  [PMID:21689223]
  Authors
Clausnitzer D, Piepersberg W, Wehmeier UF
  Title
The oxidoreductases LivQ and NeoQ are responsible for the different 6'-modifications in the aminoglycosides lividomycin and neomycin.
  Journal
J Appl Microbiol 111:642-51 (2011)
DOI:10.1111/j.1365-2672.2011.05082.x
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.95
IUBMB Enzyme Nomenclature: 2.6.1.95
ExPASy - ENZYME nomenclature database: 2.6.1.95
BRENDA, the Enzyme Database: 2.6.1.95

  All links  
Protein sequence (3)   
   UniProt (2)   
   SWISS-PROT (1)   
Protein domain (4)   
   InterPro (4)   
All databases (7)   

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