KEGG   ENZYME: 2.7.1.181Help
Entry
EC 2.7.1.181                Enzyme                                 

Name
polymannosyl GlcNAc-diphospho-ditrans,octacis-undecaprenol kinase;
WbdD;
ATP:alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol 3-phosphotransferase
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
BRITE hierarchy
Sysname
ATP:alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol 3-phosphotransferase
Reaction(IUBMB)
ATP + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol = ADP + 3-O-phospho-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol [RN:R10657]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol [CPD:C20761]
Product
ADP [CPD:C00008];
3-O-phospho-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol [CPD:C20762]
Comment
The enzyme is involved in the biosynthesis of the polymannose O-polysaccharide in the outer leaflet of the membrane of Escherichia coli serotype O9a. O-Polysaccharide structures vary extensively because of differences in the number and type of sugars in the repeat unit. The dual kinase/methylase WbdD also catalyses the methylation of 3-phospho-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)]n-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol (cf. EC 2.1.1.294, 3-O-phospho-polymannosyl GlcNAc-diphospho-ditrans,octacis-undecaprenol 3-phospho-methyltransferase).
History
EC 2.7.1.181 created 2014, modified 2017
Orthology
K18827  O-antigen chain-terminating methyltransferase
Genes
ECX: EcHS_A2169
ECR: ECIAI1_2100(wbbD)
SBG: SBG_0615(wbdD)
SBZ: A464_688
SBV: N643_03100
KPE: KPK_1679
KPJ: N559_1771
KVA: Kvar_1569
KVD: KR75_26285
KVQ: SP68_06430
KQU: AVR78_21675
EAR: CCG29189
KLW: DA718_09845
PGE: LG71_13030
YEL: LC20_07345
YRO: CH64_52
YMA: DA391_07880
SPLY: Q5A_007970
SMW: SMWW4_v1c15870
SMAC: SMDB11_0875(wbbD)
SLQ: M495_07555
SERF: L085_20550
SERM: CLM71_07390
SGL: SG1118
SOD: Sant_1470
LBQ: CKQ53_08310
TCI: A7K98_13155
XCC: XCC0602(wxcB)
XCB: XC_3631
XCA: xcc-b100_3748(wxcB)
XCP: XCR_0755
XCV: XCV3720(wxcB)
XFU: XFF4834R_chr34920(wxcB)
XAL: XALC_2708
XFR: BER92_15675
XGA: BI317_04085
XVE: BJD12_08890
XHR: XJ27_12080
XPH: XppCFBP6546_16335(XppCFBP6546P_16335)
XVA: C7V42_18460
XAN: AC801_18060
LCP: LC55x_4381
LGU: LG3211_4186
LEZ: GLE_4244
LEM: LEN_0957
PPW: PputW619_1388
PPI: YSA_02210
PPUT: L483_06425
PMON: X969_05035
PMOT: X970_05010
PSB: Psyr_0919
PSP: PSPPH_0956
PAMG: BKM19_007315
PAVL: BKM03_06250
PRH: LT40_09240
PSES: PSCI_5343
PFK: PFAS1_19955
ACX: Achr_26780
LPH: LPV_3329
LPP: lpp3019
TMC: LMI_3084
MPSY: CEK71_06980
MEC: Q7C_2302
TTI: THITH_09875
SPIU: SPICUR_08465
BSAN: CHH28_01950
NCU: F0U83_03550
OME: OLMES_0916
ACII: C4901_06700
IOD: EJO50_16185
CUH: BJN34_21700
CTI: RALTA_B1925
BCEN: DM39_688
BAM: Bamb_0757
BAC: BamMC406_0768
BCT: GEM_2648
BDL: AK34_2296
BCON: NL30_11360
BUB: BW23_855
BUM: AXG89_02425
BUI: AX768_12135
BGE: BC1002_5483
PTER: C2L65_12140
PLG: NCTC10937_04675
AMIM: MIM_c06900
DIA: Dtpsy_0563
ACK: C380_03110
ACID: CBP33_02650
CSER: CCO03_02120 CCO03_02145
HPSE: HPF_17870
MELA: C6568_04780
JAB: VN23_20315
MNR: ACZ75_23745
MASS: CR152_13210
MASZ: C9I28_24245
MTIM: DIR46_17765
MALI: EYF70_03020
EBA: ebA1588
AZD: CDA09_18445
TAK: Tharo_0944
SKU: Sulku_2113
SULS: Sdiek1_2374 Sdiek1_2375
NIS: NIS_1396
SUN: SUN_0327
DHY: DESAM_22428
DPR: Despr_0540
DTO: TOL2_C14120
DBR: Deba_0453
DAV: DESACE_00260
MOP: Mesop_2613
MAM: Mesau_05027
SIT: TM1040_3846
SPSE: SULPSESMR1_05004
AFE: Lferr_2594
AFR: AFE_2979
AFI: Acife_0129
AFJ: AFERRID_09220
ANX: ACH33_01805
COHN: KCTCHS21_50790
DRU: Desru_3668
DAI: Desaci_4417
SAY: TPY_2532
SAP: Sulac_3285
CELZ: E5225_05805
FAL: FRAAL4063
SACC: EYD13_00425
SYC: syc2192_d
SYF: Synpcc7942_1903
SYU: M744_06920
PMN: PMN2A_1230
PME: NATL1_21081
PRM: EW15_2188
GEN: GM3709_505
MAR: MAE_13200
CSN: Cyast_1719
CYL: AA637_02930
GEI: GEI7407_2321
RIV: Riv7116_5910
FIS: FIS3754_41880
NCN: BZZ01_22195
CTHE: Chro_5246
SUS: Acid_5620
ABAC: LuPra_04956
AAE: aq_1079
HHO: HydHO_1539
HYS: HydSN_1582
DTE: Dester_1313
DIN: Selin_0224
AFU: AF_0046
MAC: MA_1178
MBY: MSBRM_0546
MBAR: MSBR2_1832
MVC: MSVAZ_3023
MEK: MSKOL_3013
MEF: MSWH1_2209
MEQ: MSWHS_2872
MTHE: MSTHC_0809
MTHR: MSTHT_2473
MFZ: AOB57_003055
MHZ: Metho_1287
MTP: Mthe_1074
MCJ: MCON_1848
MHI: Mhar_1105
MPI: Mpet_1402
MPD: MCP_1036
MEZ: Mtc_0614(metW)
RCI: RCIX214
 » show all
Taxonomy
Reference
1  [PMID:15184370]
  Authors
Clarke BR, Cuthbertson L, Whitfield C
  Title
Nonreducing terminal modifications determine the chain length of polymannose O antigens of Escherichia coli and couple chain termination to polymer export via an ATP-binding cassette transporter.
  Journal
J Biol Chem 279:35709-18 (2004)
DOI:10.1074/jbc.M404738200
  Sequence
Reference
2  [PMID:19734145]
  Authors
Clarke BR, Greenfield LK, Bouwman C, Whitfield C
  Title
Coordination of polymerization, chain termination, and export in assembly of the  Escherichia coli lipopolysaccharide O9a antigen in an ATP-binding cassette transporter-dependent pathway.
  Journal
J Biol Chem 284:30662-72 (2009)
DOI:10.1074/jbc.M109.052878
  Sequence
Reference
3  [PMID:21990359]
  Authors
Clarke BR, Richards MR, Greenfield LK, Hou D, Lowary TL, Whitfield C
  Title
In vitro reconstruction of the chain termination reaction in biosynthesis of the  Escherichia coli O9a O-polysaccharide: the chain-length regulator, WbdD, catalyzes the addition of methyl phosphate to the non-reducing terminus of the growing glycan.
  Journal
J Biol Chem 286:41391-401 (2011)
DOI:10.1074/jbc.M111.295857
  Sequence
Reference
4  [PMID:25422321]
  Authors
Liston SD, Clarke BR, Greenfield LK, Richards MR, Lowary TL, Whitfield C
  Title
Domain interactions control complex formation and polymerase specificity in the biosynthesis of the Escherichia coli O9a antigen.
  Journal
J Biol Chem 290:1075-85 (2015)
DOI:10.1074/jbc.M114.622480
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.181
IUBMB Enzyme Nomenclature: 2.7.1.181
ExPASy - ENZYME nomenclature database: 2.7.1.181
BRENDA, the Enzyme Database: 2.7.1.181

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Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (3)   
   KEGG REACTION (1)   
   KEGG RCLASS (2)   
Gene (1673)   
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   KEGG GENES (169)   
   KEGG MGENES (988)   
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Protein sequence (4)   
   UniProt (2)   
   SWISS-PROT (2)   
DNA sequence (2)   
   GenBank (1)   
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All databases (1686)   

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