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Entry
EC 2.7.1.190                Enzyme                                 

Name
aminoglycoside 2''-phosphotransferase;
aphD (gene name);
APH(2'');
aminoglycoside (2'') kinase;
gentamicin kinase (ambiguous);
gentamicin phosphotransferase (ambiguous)
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
BRITE hierarchy
Sysname
GTP:gentamicin 2''-O-phosphotransferase
Reaction(IUBMB)
GTP + gentamicin = GDP + gentamicin 2''-phosphate [RN:R11229]
Reaction(KEGG)
Substrate
GTP [CPD:C00044];
gentamicin [CPD:C00505]
Product
GDP [CPD:C00035];
gentamicin 2''-phosphate
Comment
Requires Mg2+. This bacterial enzyme phosphorylates many 4,6-disubstituted aminoglycoside antibiotics that have a hydroxyl group at position 2'', including kanamycin A, kanamycin B, tobramycin, dibekacin, arbekacin, amikacin, gentamicin C, sisomicin and netilmicin. In most, but not all, cases the phosphorylation confers resistance against the antibiotic. Some forms of the enzyme use ATP as a phosphate donor in appreciable amount. The enzyme is often found as a bifunctional enzyme that also catalyses 6'-aminoglycoside N-acetyltransferase activity. The bifunctional enzyme is the most clinically important aminoglycoside-modifying enzyme in Gram-positive bacteria, responsible for high-level resistance in both Enterococci and Staphylococci.
History
EC 2.7.1.190 created 2015
Orthology
K17910  aminoglycoside 2''-phosphotransferase
K19883  bifunctional aminoglycoside 6'-N-acetyltransferase / aminoglycoside 2''-phosphotransferase
Genes
CCC: G157_08875(aph(2'')-Ig)
BAN: BA_1397
BAR: GBAA_1397
BAT: BAS1292
BAH: BAMEG_3197
BAI: BAA_1465
BAX: H9401_1311
BANT: A16_14430
BANR: A16R_14600
BANS: BAPAT_1316
BANV: DJ46_223
BCE: BC1379
BCZ: BCE33L1266(aph) BCE33L1900(aph)
BCQ: BCQ_1453(aph)
BCX: BCA_1433
BNC: BCN_1354
BCF: bcf_06980
BTL: BALH_1236
BTT: HD73_1610
BTHI: BTK_08195
BTM: MC28_0615
BTI: BTG_13850
BTW: BF38_2599
BWW: bwei_3621
BMYO: BG05_4514
BMYC: DJ92_4030
BCL: ABC0207
GEA: GARCT_02923(aacA-aphD)
SAV: SAVP026(aacA)
SUW: SATW20_20700(aacA-aphD)
SAUR: SABB_05319
SER: SERP1585(aacA)
SHA: SH1611(aacAaphD)
SSCZ: RN70_01460
PSAB: PSAB_19460
PRI: PRIO_3622
AAC: Aaci_2829
AAD: TC41_3175
SMB: smi_1704(aacA-aphD)
EFA: EFA0061
EAV: EH197_00430(aph(2'')-Ia)
CSD: Clst_1515
CCEL: CCDG5_2009
NFA: NFA_31340(aph(2''))
SNA: Snas_6439
CWO: Cwoe_4461
 » show all
Taxonomy
Reference
1  [PMID:3015884]
  Authors
Ferretti JJ, Gilmore KS, Courvalin P
  Title
Nucleotide sequence analysis of the gene specifying the bifunctional 6'-aminoglycoside acetyltransferase 2"-aminoglycoside phosphotransferase enzyme in Streptococcus faecalis and identification and cloning of gene regions specifying the two activities.
  Journal
J Bacteriol 167:631-8 (1986)
DOI:10.1128/JB.167.2.631-638.1986
  Sequence
Reference
2  [PMID:23115238]
  Authors
Frase H, Toth M, Vakulenko SB
  Title
Revisiting the nucleotide and aminoglycoside substrate specificity of the bifunctional aminoglycoside acetyltransferase(6')-Ie/aminoglycoside phosphotransferase(2'')-Ia enzyme.
  Journal
J Biol Chem 287:43262-9 (2012)
DOI:10.1074/jbc.M112.416453
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.190
IUBMB Enzyme Nomenclature: 2.7.1.190
ExPASy - ENZYME nomenclature database: 2.7.1.190
BRENDA, the Enzyme Database: 2.7.1.190

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