KEGG   ENZYME: 2.7.1.194
Entry
EC 2.7.1.194                Enzyme                                 

Name
protein-Npi-phosphohistidine---L-ascorbate phosphotransferase;
ulaABC (gene names);
L-ascorbate PTS permease;
EIISga;
Enzyme IISga;
Enzyme IIUla
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
Sysname
protein-Npi-phospho-L-histidine:L-ascorbate Npi-phosphotransferase
Reaction(IUBMB)
[protein]-Npi-phospho-L-histidine + L-ascorbate[side 1] = [protein]-L-histidine + L-ascorbate 6-phosphate[side 2] [RN:R07671]
Reaction(KEGG)
R07671
Substrate
[protein]-Npi-phospho-L-histidine [CPD:C04261];
L-ascorbate[side 1] [CPD:C00072]
Product
[protein]-L-histidine [CPD:C00615];
L-ascorbate 6-phosphate[side 2] [CPD:C16186]
Comment
This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9 (phosphoenolpyruvate---protein phosphotransferase). Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate.
History
EC 2.7.1.194 created 1972 as EC 2.7.1.69, part transferred 2016 to EC 2.7.1.194
Pathway
ec00053  Ascorbate and aldarate metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K02821  ascorbate PTS system EIIA or EIIAB component
K02822  ascorbate PTS system EIIB component
Genes
LCQ: 111688245
ECO: b4194(ulaB) b4195(ulaC)
ECJ: JW4152(ulaB) JW4153(ulaC)
ECD: ECDH10B_4389(ulaB) ECDH10B_4390(ulaC)
EBW: BWG_3906(ulaB) BWG_3907(ulaC)
ECOK: ECMDS42_3636(ulaB) ECMDS42_3637(ulaC)
ECE: Z1088 Z5803(sgaB) Z5804(ptxA)
ECS: ECs0941 ECs5170 ECs5171
ECF: ECH74115_5710(ulaB) ECH74115_5711(ulaC)
ETW: ECSP_0961 ECSP_5294(ulaB) ECSP_5295(ulaC)
EOI: ECO111_5019(ulaC) ECO111_5020(ulaB)
EOJ: ECO26_5360(ulaB)
EOH: ECO103_4987(ulaB) ECO103_4988(ulaC)
ECK: EC55989_4751(ulaB) EC55989_4752(ulaC) EC55989_4926(vpeA) EC55989_4927(vpeB)
ECG: E2348C_4517(ulaB) E2348C_4518(ulaC)
EOK: G2583_1092 G2583_5021(ulaB) G2583_5022(ulaC)
ECW: EcE24377A_4755(ulaC)
ENA: ECNA114_4410(sgaB) ECNA114_4411(sgaA)
ECOS: EC958_4681(sgaB) EC958_4682(sgaA)
ECX: EcHS_A4438(ulaB) EcHS_A4439(ulaC)
ECM: EcSMS35_4665(ulaB) EcSMS35_4666(ulaC)
ECR: ECIAI1_4427(ulaB) ECIAI1_4428(ulaC)
ECQ: ECED1_4981(ulaB) ECED1_4982(ulaC)
EUM: ECUMN_1051 ECUMN_4727(ulaB) ECUMN_4728(ulaC)
ECT: ECIAI39_4659(ulaB) ECIAI39_4660(ulaC)
EOC: CE10_4933(ulaB) CE10_4934(ulaC)
EBR: ECB_04061(sgaB) ECB_04062(ptxA)
EBL: ECD_04061(ulaB) ECD_04062(ulaC)
EBE: B21_04023(ulaB) B21_04024(ulaC)
ECI: UTI89_C4794(sgaB) UTI89_C4795(sgaA)
EIH: ECOK1_4708(ulaB) ECOK1_4709(ulaC)
ECZ: ECS88_4780(ulaB) ECS88_4781(ulaC)
ECC: c0335 c0336 c5283(sgaB) c5284(ptxA)
EKF: KO11_22595(ulaB1) KO11_22600(ulaB2) KO11_22605(ulaC)
ELW: ECW_m4557(ulaC)
ELL: WFL_22160(ulaB1) WFL_22165(ulaB2) WFL_22170(ulaC)
ELP: P12B_c4292(ulaB) P12B_c4293(ptxA)
ELF: LF82_2373(ulaB) LF82_2374(ulac)
ECOI: ECOPMV1_04658(ulaB) ECOPMV1_04659(ulaC)
ESZ: FEM44_13635(ulaB) FEM44_13640(ulaC)
STT: t0520 t0521 t2011 t4435(sgaB) t4436(sgaA)
SEC: SCH_0877 SCH_2344 SCH_2345(ptxA) SCH_4258(sgaB) SCH_4259(ptxA)
SEG: SG0865 SG2372 SG2373 SG4226(sgaB) SG4227(ptxA)
SFL: SF4349(sgaB) SF4350(ptxA)
SFX: S4619(sgaB) S4620(ptxA)
SFV: SFV_4350(sgaB) SFV_4351(ptxA)
SFE: SFxv_4740(ulaB) SFxv_4741(ulaC)
SFT: NCTC1_04724(ulaB) NCTC1_04725(ulaC)
SSN: SSON_4376(sgaB) SSON_4377(ptxA)
SBO: SBO_4260(ptxA) SBO_4261(sgaB)
SBC: SbBS512_E4724(ulaB) SbBS512_E4725(ulaC)
SDY: SDY_4363(sgaB) SDY_4364(ptxA)
KPX: PMK1_02057(ulaB_1) PMK1_02058(ulaC_1) PMK1_02681 PMK1_02682(ulaC_2) PMK1_04086(ulaB_2) PMK1_04087(ulaC_3)
CPOT: FOB25_08840(ulaC) FOB25_08845(ulaB)
KIE: NCTC12125_02873(ulaB_1) NCTC12125_02874(ulaC_1) NCTC12125_03282(ulaC_2) NCTC12125_03283(ulaB_2)
SGOE: A8O29_020380(ulaC) A8O29_020385(ulaB)
YPK: y1617 y1618
YPM: YP_2380(ptsN1) YP_2381(sgaB)
YPZ: YPZ3_2270
YPD: YPD4_2445
YPX: YPD8_2140
YPW: CH59_105(ptsN1) CH59_106
YPJ: CH55_43 CH55_44(ptsN1)
YPV: BZ15_969 BZ15_970(ptsN1)
YPL: CH46_2547 CH46_2548(ptsN1)
YPO: BZ17_4038 BZ17_4039(ptsN1)
YPU: BZ21_1895(ptsN1) BZ21_1896
YPR: BZ20_3609 BZ20_3610(ptsN1)
YPC: BZ23_2179(ptsN1) BZ23_2180
YPF: BZ19_1960(ptsN1) BZ19_1961
YET: CH48_323(ptsN1) CH48_324
YAL: AT01_1195(ptsN1) AT01_1196
YIN: CH53_394(ptsN1) CH53_395
YRO: CH64_1215(ptsN1) CH64_1216
YRU: BD65_858(ptsN1) BD65_859
SPLY: Q5A_011575(ulaC) Q5A_011595(ulaB)
DAQ: DAQ1742_04086(ulaC) DAQ1742_04087(ulaA)
EGE: EM595_1821(ptxA) EM595_1825(ptxB)
PAJ: PAJ_1196(ulaC) PAJ_2453(sgcA)
PVA: Pvag_1291(ptxB) Pvag_1295(ptxa1) Pvag_pPag30336(ptxA) Pvag_pPag30337(ptxB)
MTHI: C7M52_00729(ulaC_1) C7M52_00730
PLU: plu1979(sgcA) plu1980(sgaB)
PAY: PAU_02590(sgaB) PAU_02591(sgcA)
PHAU: PH4a_00640 PH4a_00645(cmtB)
PRG: RB151_018600(ulaB) RB151_018610(ulaC_1) RB151_028360(ulaA_2) RB151_028370(ulaC_2)
PHEI: NCTC12003_00032(ulaC_1) NCTC12003_00033(ulaA_1) NCTC12003_02395(ulaC_2) NCTC12003_02396(ulaB)
PRJ: NCTC6933_00027(ulaC_1) NCTC6933_01594(ulaB) NCTC6933_01595(ulaC_2)
ANS: ArsFIN_48520(ulaC) ArsFIN_48530(ulaB)
ETR: ETAE_3026(sgcA) ETAE_3027 ETAE_3293(sgaB) ETAE_3294(ptxA)
ETE: ETEE_1260(ulaC) ETEE_1261(ulaB) ETEE_1521(ulaB) ETEE_1522(ulaC)
PRAG: EKN56_02385(ulaB) EKN56_02390(ulaC)
HDU: HD_1859 HD_1860(sgaT)
HAP: HAPS_1779(ulaC) HAPS_1780(ulaA)
PMV: PMCN06_0828(ulaA) PMCN06_0829(ulaC)
PMP: Pmu_08410(ulaC)
PMUL: DR93_1600(ulaA) DR93_1601(ulaC)
PDAG: 4362423_00125(ulaA) 4362423_00126(ulaC)
MSU: MS0021(ptsN) MS0022(sgaT) MS0149(ptsN) MS0150(sgaB)
MHX: MHH_c03910 MHH_c04170(ulaA) MHH_c04210(ulaC)
APL: APL_1699(ulaC) APL_1700(ulaA) APL_1715(sgaB)
APJ: APJL_1731 APJL_1732(sgaT) APJL_1748(sgaB)
APA: APP7_1760(ulaC) APP7_1761(ulaA) APP7_1775(sgaB)
RPNE: NCTC8284_01611(ulaC_1) NCTC8284_01613(ulaA_1)
VCH: VCA0245 VCA0246(ulaA)
VTA: A0949(cmtB) A0951 B0402 B0403(ulaC)
PPR: PBPRB0273(VCA0246) PBPRB0274(PTXA)
ASA: ASA_4216(sgaA) ASA_4217(sgaB)
ACAV: VI35_20535(cmtB) VI35_20540
BSON: S101395_04518(ulaB)
BCA: BCE_2982
BCQ: BCQ_2774
BMYO: BG05_3085
BPU: BPUM_1757
BPUM: BW16_09645
BPUS: UP12_09030
BMQ: BMQ_1022
BMD: BMD_1028
BMH: BMWSH_4219(sgaB)
BMEG: BG04_3312
BACW: QR42_08940
BEO: BEH_14990
BBEV: BBEV_0378 BBEV_3171(ulaC) BBEV_3172(sgaB)
GKA: GK2017
HLI: HLI_03870
VPN: A21D_02502(ulaC) A21D_02503(ulaB_1) A21D_02817(ulaB_2) A21D_03953 A21D_03954(mtlR_2)
LMOG: BN389_26090(ulaB) BN389_26100(ulaC)
LMQ: LMM7_2762(ulaB) LMM7_2763(ulaC)
JEO: JMA_35270
LLK: LLKF_1781
LLM: llmg_0866
LLC: LACR_1736
LLR: llh_4360
LLW: kw2_1587
LLJ: LG36_1576
LGR: LCGT_0640
LGV: LCGL_0660
LPK: LACPI_0209(ulaC) LACPI_0211(ulaB1) LACPI_1286(ulaB2)
STX: MGAS1882_0185(sgaB.1) MGAS1882_0186(sgaA) MGAS1882_1571(sgaB.2) MGAS1882_1572(bglG)
SPR: spr1847(PTS-EII) spr1848(PTS-EII) spr1939
SGC: A964_0210(sgaB) A964_1733(sgaA) A964_1734(sgaB)
SAGN: W903_0260 W903_1803(ulaC) W903_1804(ulaB)
SMU: SMU_271(ptxB) SMU_272(ptxA)
SMUA: SMUFR_0228(ulaB) SMUFR_0229(ulaC)
SSA: SSA_2091(ptxA) SSA_2092(ptxB)
SSUI: T15_0169(ulaB) T15_2126(ulaC) T15_2127
SGO: SGO_0422
SGT: SGGB_2097 SGGB_2102(ulaB1) SGGB_2103(ulaC1) SGGB_2108(ulaC2) SGGB_2109(ulaB2)
STK: STP_1743
STB: SGPB_1907 SGPB_1912(ulaB.1) SGPB_1913(ulaC.1) SGPB_1918(ulaC.2) SGPB_1919(ulaB.2)
STF: Ssal_01502(ulaB)
SIE: SCIM_1484
SIB: SIR_1656(ptxA) SIR_1657
LPL: lp_3137(pts25B) lp_3541(pts34B) lp_3542(pts34A)
LPT: zj316_0155(pts34B) zj316_0156(pts34A)
LRH: LGG_00400(ulaA) LGG_00401(ulaB) LGG_02730(sgaB)
LRL: LC705_00387(ulaB) LC705_00432(sgaA) LC705_00434(sgaB) LC705_00465(cmtB) LC705_00466(rmpB) LC705_02734(sgaB)
EFA: EF1126 EF1128(sgaB) EF2965
EFN: DENG_00604(ulaC) DENG_00605(ulaB) DENG_01263(ulaC) DENG_01265(ulaB) DENG_02848(ulaB)
MPX: MPD5_1556
LGS: LEGAS_1795(ulaB)
STH: STH2309
CBAR: PATL70BA_2883(ulaB)
TTE: TTE0305(SgaB)
THX: Thet_0280
TIT: Thit_0262
TSH: Tsac_0264
VRM: 44547418_01426(ulaB) 44547418_01427(ulaC)
ERH: ERH_0724(ulaB)
MPN: MPN494(yjfU) MPN495
MPM: MPNA4940(sgaA) MPNA4950(sgaB)
MPU: MYPU_1980(MYPU_1980) MYPU_5950(MYPU_5950) MYPU_5960(MYPU_5960)
MPE: MYPE4480(MYPE4480) MYPE4490(MYPE4490) MYPE7190(MYPE7190) MYPE7200(MYPE7200)
MMY: MSC_0394(pts) MSC_0395
MML: MLC_3750(pts) MLC_3760
MCAP: MCCPF38_00672(ulaA) MCCPF38_00673(ulaC)
MHY: mhp386(sgaA) mhp387(sgaB) mhp572 mhp573
MHYO: MHL_2824(sgaB) MHL_2830 MHL_3343(sgaA)
MAA: MAG6360 MAG6370(sgaB)
MAL: MAGa7320 MAGa7330(sgaB)
MHR: MHR_0457(sgaA) MHR_0458
MHV: Q453_0519(ulaC) Q453_0520(ulaB)
MFM: MfeM64YM_0030(sgaB) MfeM64YM_0031(sgaA)
MBH: MMB_0682 MMB_0683(sgaB)
MBI: Mbov_0721(ulaC) Mbov_0722(ulaB)
MCY: MCYN_0234(MCYN0234) MCYN_0235(MCYN0235) MCYN_0266(MCYN0266) MCYN_0267(MCYN0267)
MBC: MYB_01215(ulaB) MYB_01220
EFR: EFREU_v1c02770(sgaT) EFREU_v1c02780(sgaB)
STAI: STAIW_v1c06940(sgaT) STAIW_v1c06950(sgaA)
SMIR: SMM_0796
SMIA: P344_04790
SPRN: S100390_v1c04890(sgaA)
SPIT: STU14_v1c04890(sgaA)
MBJ: KQ51_00704(ulaB_1) KQ51_01338(ulaB_2) KQ51_01340(ulaC)
MVQ: MYVA_5824(rmpA)
CGL: NCgl2934(Cgl3037)
CGB: cg3366(rmpA)
CGU: WA5_2934(RmpA)
CGT: cgR_2923
CGM: cgp_3366(rmpA)
CGJ: AR0_14710
CDIP: ERS451417_02358(ulaC)
CPL: Cp3995_0084(sgaA)
CPP: CpP54B96_0087(sgaA)
CPZ: CpPAT10_0082(sgaA)
COR: Cp267_0091(sgaA)
COD: Cp106_0083(sgaA)
COS: Cp4202_0081(sgaA)
CPSF: CPTC_00586
CUN: Cul210932_0130(sgaA)
CUS: CulFRC11_0125(sgaA)
CUQ: Cul210931_0124(sgaA)
CUZ: Cul05146_0129(sgaA)
CUJ: CUL131002_0113(sgaA)
CSP: WM42_0749
CGV: CGLAU_11595(ulaC)
CAQU: CAQU_11785
CAMG: CAMM_04675
CMIN: NCTC10288_01931(ulaC)
CPEG: CPELA_04365(ulaC)
CEE: CENDO_00435(ulaC)
SCO: SCO0137(SCJ21.18c)
SALB: XNR_0311
STRE: GZL_02837
SFK: KY5_0277c
KRH: KRH_22560
DCO: SAMEA4475696_1768(ulaC)
PAC: PPA0447
PAW: PAZ_c04600(ulaC)
PACC: PAC1_02270
CACN: RN83_02720
CGRN: 4412665_00805(ulaC)
STRR: EKD16_07085(ulaB) EKD16_07090(ulaC)
BBF: BBB_0026(ulaC) BBB_0027(ulaB)
BSCA: BBSC_2540
BPW: WESB_0931 WESB_2433(sgaB) WESB_2434(ptsN1) WESB_2438(sgaB1)
BIP: Bint_0481 Bint_1774(sgaB)
THS: TES1_0693
 » show all
Reference
1  [PMID:12644495]
  Authors
Zhang Z, Aboulwafa M, Smith MH, Saier MH Jr
  Title
The ascorbate transporter of Escherichia coli.
  Journal
J Bacteriol 185:2243-50 (2003)
DOI:10.1128/JB.185.7.2243-2250.2003
  Sequence
[eco:b4194 b4195]
Reference
2  [PMID:15153772]
  Authors
Hvorup R, Chang AB, Saier MH Jr
  Title
Bioinformatic analyses of the bacterial L-ascorbate phosphotransferase system permease family.
  Journal
J Mol Microbiol Biotechnol 6:191-205 (2003)
DOI:10.1159/000077250
  Sequence
[eco:b4194 b4195]
Reference
3  [PMID:25686089]
  Authors
Luo P, Yu X, Wang W, Fan S, Li X, Wang J
  Title
Crystal structure of a phosphorylation-coupled vitamin C transporter.
  Journal
Nat Struct Mol Biol 22:238-41 (2015)
DOI:10.1038/nsmb.2975
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.194
IUBMB Enzyme Nomenclature: 2.7.1.194
ExPASy - ENZYME nomenclature database: 2.7.1.194
BRENDA, the Enzyme Database: 2.7.1.194

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