EC                Enzyme                                 

protein-Npi-phosphohistidine---lactose phosphotransferase;
lacEF (gene names);
lactose PTS permease;
Enzyme IILac
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
protein-Npi-phospho-L-histidine:lactose Npi-phosphotransferase
[protein]-Npi-phospho-L-histidine + lactose[side 1] = [protein]-L-histidine + lactose 6'-phosphate[side 2] [RN:R04393 R06237]
R04393 R06237(G)
lactose[side 1]
[protein]-L-histidine [CPD:C00615];
lactose 6'-phosphate[side 2]
This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC (phosphoenolpyruvate---protein phosphotransferase). Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate.
EC created 1972 as EC, part transferred 2016 to EC
ec00052  Galactose metabolism
ec01100  Metabolic pathways
K02786  lactose PTS system EIIA component
K02788  lactose PTS system EIICB component
SDO: SD1155_00680 SD1155_00685
BAY: RBAM_012160 RBAM_012170
BAQ: BACAU_1168(lacE) BACAU_1169(lacF)
BYA: BANAU_1148(lacE) BANAU_1149(lacF)
BAMP: B938_06200 B938_06205
BAML: BAM5036_1122(lacE) BAM5036_1123(lacF)
BAMA: RBAU_1170(lacE) RBAU_1171(lacF)
BAMN: BASU_1150(lacE) BASU_1151(lacF)
BAMB: BAPNAU_2575(lacF) BAPNAU_2576(lacE)
BAMT: AJ82_06820 AJ82_06825
BAMY: V529_11580(lacE) V529_11590(lacF)
BMP: NG74_01237(lacE) NG74_01238(lacF_1)
BAO: BAMF_1294(lacE) BAMF_1295(lacF)
BAZ: BAMTA208_11100(lacF) BAMTA208_11105(lacE)
BQL: LL3_01303(lacE) LL3_01304(lacF)
BXH: BAXH7_02272(lacF) BAXH7_02273(lacE)
BQY: MUS_1295(lacE) MUS_1296(lacF)
SAU: SA1992(lacE) SA1993(lacF)
SAV: SAV2190(lacE) SAV2191(lacF)
SAW: SAHV_2174(lacE) SAHV_2175(lacF)
SAM: MW2116(lacE) MW2117(lacF)
SAR: SAR2281(lacE) SAR2282(lacF)
SAC: SACOL2181(lacE) SACOL2182(lacF)
SAX: USA300HOU_2183(lacE) USA300HOU_2184(lacF)
SAA: SAUSA300_2150(lacE) SAUSA300_2151(lacF)
SAE: NWMN_2094(lacE) NWMN_2095(lacF)
SAD: SAAV_2251(lacE) SAAV_2252(lacF)
SUV: SAVC_09840(lacE) SAVC_09845(lacF)
SUZ: MS7_2213(lacE) MS7_2214(lacF)
SUW: SATW20_23280(lacE) SATW20_23290(lacF)
SAUE: RSAU_002034(lacE) RSAU_002035(lacF)
SAUS: SA40_1945(lacE) SA40_1946(lacF)
SAUU: SA957_2029(lacE) SA957_2030(lacF)
SAUG: SA268_2100(lacE) SA268_2101(lacF)
SAUZ: SAZ172_2294(lacE) SAZ172_2295(lacF)
SAUT: SAI1T1_2016310(lacE)
SAUJ: SAI2T2_1016320(lacE)
SAUK: SAI3T3_1016310(lacE)
SAUQ: SAI4T8_1016320(lacE)
SAUV: SAI7S6_1016310(lacE)
SAUW: SAI5S5_1016250(lacE)
SAUX: SAI6T6_1016260(lacE)
SAUY: SAI8T7_1016290(lacE)
SAB: SAB2071c(lacE) SAB2072c(lacF)
SUY: SA2981_2128(lacE) SA2981_2129(lacF)
SAUB: C248_2228(lacE) C248_2229(lacF)
SAUC: CA347_2279(lacE) CA347_2280(lacF)
SAUR: SABB_01469(lacF) SABB_03032(lacE)
SER: SERP1790(lacE) SERP1791(lacF)
SEPS: DP17_734(lacE) DP17_735(lacF)
SHA: SH0846(lacF) SH0847(lacE)
SCA: SCA_0667(lacF) SCA_0668(lacE)
SLN: SLUG_08240(lacF)
SDT: SPSE_0619(lacF) SPSE_0620(lacE)
SHU: SHYC_03365(lacF) SHYC_03370(lacE)
SCAP: AYP1020_1384(lacE) AYP1020_1385(lacF)
SSCZ: RN70_06905
LMOE: BN418_1054
LMOB: BN419_1056
LIV: LIV_0816
LLR: llh_14025(lacE) llh_14030(lacF)
LLI: uc509_p8018(lacE) uc509_p8019(lacF)
SPY: SPy_1917(lacE) SPy_1918(lacF)
SPZ: M5005_Spy1633(lacE) M5005_Spy1634(lacF)
SPYM: M1GAS476_0299(lacF) M1GAS476_0300(lacE)
SPYA: A20_1683c(lacE) A20_1684c(lacF)
SPG: SpyM3_1654(lacE) SpyM3_1655(lacF)
SPF: SpyM51608(lacE) SpyM51609(lacF)
SPB: M28_Spy1623(lacE) M28_Spy1624(lacF)
STG: MGAS15252_1483(lacE) MGAS15252_1484(lacF)
STX: MGAS1882_1544(lacE) MGAS1882_1545(lacA)
SOZ: Spy49_1585c(lacE) Spy49_1586c(lacF)
SPYH: L897_08215
SPN: SP_0476(lacF-1) SP_0478(lacE-1) SP_1185(lacE-2) SP_1186(lacF-2)
SPD: SPD_0426(lacF-1) SPD_0428(lacE-1) SPD_1047(lacE-2) SPD_1048(lacF-2)
SPR: spr0423(PTS-EII) spr0425(PTS-EII) spr1070(lacE) spr1071(lacF)
SNE: SPN23F10860(lacE2) SPN23F10870(lacF)
SNX: SPNOXC10640(lacE2) SPNOXC10650(lacF)
SPNU: SPN034183_10640(lacE2) SPN034183_10650(lacF)
SPNM: SPN994038_10530(lacE2) SPN994038_10540(lacF)
SPNO: SPN994039_10540(lacE2) SPN994039_10550(lacF)
SMU: SMU_1491(lacE) SMU_1492(lacF)
SMC: SmuNN2025_0618(lacF) SmuNN2025_0619(lacE)
SMJ: SMULJ23_0627(lacF) SMULJ23_0628(lacE)
SSA: SSA_1693(lacE) SSA_1694(lacF)
SSB: SSUBM407_0516(lacF2) SSUBM407_0517(lacE2) SSUBM407_0886(lacF1) SSUBM407_0887(lacE1)
SSI: SSU0892(lacE) SSU0893(lacF)
SSS: SSUSC84_0937(lacE) SSUSC84_0938(lacF)
SSQ: SSUD9_1215 SSUD9_1216(lacF1)
SSUI: T15_0915(lacF) T15_0916
SGO: SGO_1513(lacE) SGO_1514(lacF)
SEZ: Sez_0459(lacF) Sez_0460(lacE)
SEQ: SZO_15230(lacE) SZO_15240(lacF)
SUB: SUB0798(lacF2) SUB0799(lacE2) SUB1451(lacF1)
SDA: GGS_0828(lacF) GGS_0829(lacE)
SMB: smi_1037(lacF) smi_1038(lacE)
SOR: SOR_1084(lacE) SOR_1085(lacF)
SCF: Spaf_1252(lacE) Spaf_1253(lacF2)
SMN: SMA_0209(lacF) SMA_0210(lacE) SMA_1158(lacE) SMA_1159(lacF)
SIE: SCIM_1141(lacE) SCIM_1142(lacF)
SIB: SIR_0450(lacF) SIR_0451(lacE)
SIU: SII_0434(lacF) SII_0435(lacE)
SANG: SAIN_1265(lacE) SAIN_1266(lacF)
SANC: SANR_1490(lacE) SANR_1491(lacF)
SCG: SCI_1406(lacE) SCI_1407(lacF)
SCON: SCRE_1363(lacE) SCRE_1364(lacF)
SCOS: SCR2_1363(lacE) SCR2_1364(lacF)
STRG: SRT_06290(lacF) SRT_06300(lacE)
SFER: NCTC12278_00093(lacF) NCTC12278_00094(lacE)
SCAI: NCTC12191_01616(lacE) NCTC12191_01617(lacF_2)
LJF: FI9785_571(lacF) FI9785_572(lacE)
LDE: LDBND_1645(lacE) LDBND_1647(lacF)
LCS: LCBD_0732 LCBD_0734(celCD)
LCE: LC2W_0732 LC2W_0734(celCD) LC2W_p06(lacE) LC2W_p08(lacF)
LCW: BN194_07310(lacE) BN194_07330(lacF_2)
LCB: LCABL_07270(lacE) LCABL_07290(lacF)
LRH: LGG_00649(lacE) LGG_00652(lacF)
LRL: LC705_00312(lacE) LC705_00314(lacF) LC705_00622(lacE) LC705_00624(lacF) LC705_p00063(lacE) LC705_p00065(lacF)
LRA: LRHK_321(lacE) LRHK_323(lacF) LRHK_640 LRHK_642(lacF)
EFL: EF62_0868(lacE) EF62_0869(lacF)
EFN: DENG_00523(lacF) DENG_00524(lacE)
EMU: EMQU_1647(lacE) EMQU_1648
ESG: EsVE80_18890(lacE) EsVE80_18900(lacF)
ECEC: NCTC12421_00222(lacF) NCTC12421_00223(lacE)
CAC: CA_C2964(lacE) CA_C2965(lacF)
CAE: SMB_G3000(lacE) SMB_G3001(lacF)
CAY: CEA_G2971(lacE) CEA_G2972(lacF)
CSB: CLSA_c41090(lacE) CLSA_c41100(lacF)
CBV: U729_2187(lacF) U729_2188(lacE)
TAE: TepiRe1_0056(lacF)
SRI: SELR_04240(lacE) SELR_04320(lacF)
FIT: Fi14EGH31_18970(lacE1) Fi14EGH31_18980(lacF)
ELJ: ELUMI_v1c08110(lacF) ELUMI_v1c08130(celB)
STAI: STAIW_v1c07630(celC)
SERI: SERIO_v1c00550(lacF)
SLL: SLITO_v1c06870(lacF) SLITO_v1c06890(lacE)
SCLA: SCLARK_00209(celC) SCLARK_00215(celC) SCLARK_00223(celC)
LSZ: JCM16776_0562(lacF) JCM16776_0563(lacE)
 » show all
1  [PMID:11947593]
Hengstenberg W
Solubilization of the membrane bound lactose specific component of the staphylococcal PEP dependant phosphotransferase system.
FEBS Lett 8:277-280 (1970)
2  [PMID:6480107]
Vadeboncoeur C, Proulx M
Lactose transport in Streptococcus mutans: isolation and characterization of factor IIIlac, a specific protein component of the phosphoenolpyruvate-lactose phosphotransferase system.
Infect Immun 46:213-9 (1984)
3  [PMID:2824493]
Breidt F Jr, Hengstenberg W, Finkeldei U, Stewart GC
Identification of the genes for the lactose-specific components of the phosphotransferase system in the lac operon of Staphylococcus aureus.
J Biol Chem 262:16444-9 (1987)
4  [PMID:2125052]
de Vos WM, Boerrigter I, van Rooyen RJ, Reiche B, Hengstenberg W
Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis.
J Biol Chem 265:22554-60 (1990)
5  [PMID:7737179]
Peters D, Frank R, Hengstenberg W
Lactose-specific enzyme II of the phosphoenolpyruvate-dependent phosphotransferase system of Staphylococcus aureus. Purification of the histidine-tagged transmembrane component IICBLac and its hydrophilic IIB domain by metal-affinity chromatography, and functional characterization.
Eur J Biochem 228:798-804 (1995)
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