KEGG   ENZYME: 2.7.1.228
Entry
EC 2.7.1.228                Enzyme                                 
Name
mannosyl-inositol-phosphoceramide inositolphosphotransferase;
IPT1 (gene name)
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
Sysname
1-phosphatidyl-1D-myo-inositol:(4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-{[6-O-(alpha-D-mannosyl)-1D-myo-inositol-1-O-yl]hydroxyphosphoryl}sphinganine phosphoinositoltransferase
Reaction(IUBMB)
1-phosphatidyl-1D-myo-inositol + a (4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-{[6-O-(alpha-D-mannosyl)-1D-myo-inositol-1-O-yl]hydroxyphosphoryl}sphinganine = 1,2-diacyl-sn-glycerol + a (4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-[(6-O-{6-O-[(1D-myo-inositol-1-O-yl)hydroxyphosphoryl]-alpha-D-mannosyl}-1D-myo-inositol-1-O-yl)hydroxyphosphoryl]sphinganine
Substrate
1-phosphatidyl-1D-myo-inositol [CPD:C01194];
(4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-{[6-O-(alpha-D-mannosyl)-1D-myo-inositol-1-O-yl]hydroxyphosphoryl}sphinganine
Product
1,2-diacyl-sn-glycerol [CPD:C00641];
(4R)-4-hydroxy-N-[(2R)-2-hydroxy-very-long-chain-acyl]-1-O-[(6-O-{6-O-[(1D-myo-inositol-1-O-yl)hydroxyphosphoryl]-alpha-D-mannosyl}-1D-myo-inositol-1-O-yl)hydroxyphosphoryl]sphinganine
Comment
This enzyme catalyses the ultimate reaction in the yeast sphingolipid biosynthesis pathway. It transfers a second phosphoinositol group to mannosyl-inositol-phospho-alpha-hydroxyphytoceramide (MIPC), forming the final and most abundant yeast sphingolipid, mannosyl-diphosphoinositol-ceramide (MIP2C).
History
EC 2.7.1.228 created 2019
Orthology
K22725  mannosyl-inositol-phosphoceramide inositolphosphotransferase
Genes
SCEYDR072C(IPT1)
ERCEcym_5553
KLAKLLA0_E10187g
KMXKLMA_20024(IPT1)
LTHKLTH0H07590g
VPOKpol_1032p31
ZROZYRO0G14718g
CGRCAGL0G05313g
NCSNCAS_0B04230(NCAS0B04230)
NDINDAI_0A03650(NDAI0A03650)
TPFTPHA_0A02150(TPHA0A02150)
TBLTBLA_0F03590(TBLA0F03590)
TDLTDEL_0B04170(TDEL0B04170)
TGBHG536_0D02150
KAFKAFR_0C02620(KAFR0C02620)
PPAPAS_chr3_0735
DHADEHA2F25520g
PICPICST_49558 PICST_61648 PICST_63545 PICST_78790
PGUPGUG_03091 PGUG_05895
SPAASPAPADRAFT_58326 SPAPADRAFT_58414
LELLELG_00825 LELG_00893
CALCAALFM_C109120WA(IPT1)
CTPCTRG_04128
COTCORT_0A07460 CORT_0A08040
CDUCD36_08590
CTENCANTEDRAFT_124983
CLUCLUG_04090
CLUSA9F13_01g00176
CAURCJI97_004150
BNNFOA43_001983
SSLSS1G_02388
BFUBCIN_13g02520(Bcipt1)
MBEMBM_07403
PSCOLY89DRAFT_727904
GLZGLAREA_01667
 » show all
Reference
1  [PMID:9368028]
  Authors
Dickson RC, Nagiec EE, Wells GB, Nagiec MM, Lester RL
  Title
Synthesis of mannose-(inositol-P)2-ceramide, the major sphingolipid in Saccharomyces cerevisiae, requires the IPT1 (YDR072c) gene.
  Journal
J Biol Chem 272:29620-5 (1997)
DOI:10.1074/jbc.272.47.29620
  Sequence
[sce:YDR072C]
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.228
IUBMB Enzyme Nomenclature: 2.7.1.228
ExPASy - ENZYME nomenclature database: 2.7.1.228
BRENDA, the Enzyme Database: 2.7.1.228

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