KEGG   ENZYME: 2.7.1.238
Entry
EC 2.7.1.238                Enzyme                                 
Name
phenol phosphorylase;
phenylphosphate synthase
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
Sysname
ATP:phenol phosphotransferase (AMP-forming)
Reaction(IUBMB)
ATP + phenol + H2O = AMP + phenyl phosphate + phosphate [RN:R05625]
Reaction(KEGG)
R05625
Substrate
ATP [CPD:C00002];
phenol [CPD:C00146];
H2O [CPD:C00001]
Product
AMP [CPD:C00020];
phenyl phosphate [CPD:C02734];
phosphate [CPD:C00009]
Comment
The enzyme, characterized from the bacterium Thauera aromatica, catalyses the first step in an anaerobic phenol degradation pathway. The enzyme, composed of three subunits, transfers the beta-phosphoryl from ATP to phenol, forming phenyl phosphate, AMP, and phosphate [1]. During catalysis a diphosphoryl group is transferred from ATP to a histidine residue in one of the enzyme's subunits, from which phosphate is cleaved to render the reaction unidirectional. The remaining histidine phosphate subsequently serves as the actual phosphorylation agent [2].
History
EC 2.7.1.238 created 2022
Pathway
ec00627  Aminobenzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K25936  phenol phosphorylase subunit alpha
K25937  phenol phosphorylase subunit beta
K25938  phenol phosphorylase subunit delta gamma
Genes
EBAebA3143(ppsB) ebA3144(ppsA) ebD7(ppsC)
ABREpbN1_19200(ppsC) pbN1_19210(ppsB) pbN1_19220(ppsA)
APETToN1_08850(ppsA) ToN1_08860(ppsB) ToN1_08870(ppsC)
AZAAZKH_p0129(ppsA) AZKH_p0130(ppsB) AZKH_p0131(ppsC)
AZIAzCIB_2684 AzCIB_2685 AzCIB_2686
AZDCDA09_14585 CDA09_14590 CDA09_14595
TAKTharo_2774 Tharo_2775 Tharo_2776
DDZDSYM_29710 DSYM_29720 DSYM_29730
GMEGmet_2100 Gmet_2101 Gmet_2104
GEOGeob_0138 Geob_0141 Geob_0142
DTOTOL2_C15950(ppsA) TOL2_C15960(ppsB) TOL2_C16020(ppsC)
DTIDesti_0829 Desti_0830 Desti_0834
DBRDeba_2753
SBAEDSM104329_00598(cmdD_1) DSM104329_02332 DSM104329_02333(ppsA_2)
 » show all
Reference
1  [PMID:15547277]
  Authors
Schmeling S, Narmandakh A, Schmitt O, Gad'on N, Schuhle K, Fuchs G.
  Title
Phenylphosphate synthase: a new phosphotransferase catalyzing the first step in anaerobic phenol metabolism in Thauera aromatica.
  Journal
J Bacteriol 186:8044-57 (2004)
DOI:10.1128/JB.186.23.8044-8057.2004
  Sequence
Reference
2  [PMID:16980461]
  Authors
Narmandakh A, Gad'on N, Drepper F, Knapp B, Haehnel W, Fuchs G.
  Title
Phosphorylation of phenol by phenylphosphate synthase: role of histidine phosphate in catalysis.
  Journal
J Bacteriol 188:7815-22 (2006)
DOI:10.1128/JB.00785-06
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.238
IUBMB Enzyme Nomenclature: 2.7.1.238
ExPASy - ENZYME nomenclature database: 2.7.1.238
BRENDA, the Enzyme Database: 2.7.1.238

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