KEGG   ENZYME: 2.7.1.95
Entry
EC 2.7.1.95                 Enzyme                                 

Name
kanamycin kinase;
neomycin-kanamycin phosphotransferase
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
Sysname
ATP:kanamycin 3'-O-phosphotransferase
Reaction(IUBMB)
ATP + kanamycin = ADP + kanamycin 3'-phosphate
Reaction(KEGG)
(other) R01888
Substrate
ATP [CPD:C00002];
kanamycin [CPD:C00304]
Product
ADP [CPD:C00008];
kanamycin 3'-phosphate [CPD:C03281]
Comment
Also acts on the antibiotics neomycin, paromomycin, neamine, paromamine, vistamycin and gentamicin A. An enzyme from Pseudomonas aeruginosa also acts on butirosin.
History
EC 2.7.1.95 created 1976
Orthology
K00897  kanamycin kinase
K19272  aminoglycoside 3'-phosphotransferase I
K19274  aminoglycoside 3'-phosphotransferase VI
K19299  aminoglycoside 3'-phosphotransferase III
K19300  aminoglycoside 3'-phosphotransferase II
K19315  aminoglycoside 3'-phosphotransferase XV
K19543  aminoglycoside 3'-phosphotransferase
Genes
MPHA: 105840509
BMY: Bm1_10645
EHI: EHI_117050
EBW: BWG_3698
EOI: ECO111_p1-120
EOJ: ECO26_p2-04
ECM: EcSMS35_A0159
SEC: SCH_026(aph)
ECLE: ECNIH2_23600 ECNIH2_26045
ECLY: LI62_013010 LI62_11650
KPN: KPN_pKPN5p08194(aphA)
KPZ: KPNIH27_25655
KPG: KPNIH32_28420
KPC: KPNIH10_26765
KPB: FH42_25875
KPNE: KU54_00025
KPNU: LI86_26895
KOX: KOX_20540
KOE: A225_2977
KOY: J415_17065
KOM: HR38_18875
KOK: KONIH1_14625 KONIH1_30465
KOC: AB185_22765
CFD: CFNIH1_24930
CWE: CO701_09595
CPAR: CUC49_08460
KIE: NCTC12125_04789(neo)
KIN: AB182_03320
SMAF: D781_0520
SRZ: AXX16_0005
SERM: CLM71_02525
PANP: PSNIH2_20340
PCOL: F1325_16150(aph(3')-I)
PCIB: F9282_16450(aph(3')-VI)
PSTA: BGK56_21235
PMP: Pmu_02740(aphA1)
MHX: MHH_c22550(aphA1)
AIO: EXH44_00925
GAN: UMN179_01162
BTO: WQG_1070
BTRH: F543_22790
BTRA: F544_3130
SML: Smlt2120
SMT: Smal_1717
BUJ: BurJV3_1756
SMZ: SMD_1909
STEN: CCR98_09260
STEM: CLM74_09685
STES: MG068_09120(aph(3')-II)
LMB: C9I47_1673
LUE: DCD74_02565(aph(3')-XV)
DKO: I596_3655
PAE: PA4119(aph)
PAEV: N297_4251(neo)
PAEI: N296_4251(neo)
PAU: PA14_10670(aph)
PAP: PSPA7_0973(aph) PSPA7_3721
PAG: PLES_08521(aph)
PNC: NCGM2_5320(aph)
PAEB: NCGM1900_0841(aph)
PSG: G655_04105
PRP: M062_21725
PAEP: PA1S_04225
PAER: PA1R_gp2010
PAEM: U769_04250
PAEL: T223_04160
PAES: SCV20265_0848
PAEU: BN889_04573(aph)
PAEG: AI22_29390
PAEC: M802_4249(neo)
PAEO: M801_4117(neo)
PPUH: B479_26894
PFB: VO64_1917
PTV: AA957_19685
PFW: PF1751_v1c45260
PFF: PFLUOLIPICF713405
PSTU: UIB01_10035
PSIL: PMA3_18220
PYM: AK972_1650
PUM: HGP31_03940
PGY: AWU82_21750(aph(3')-I)
ABY: ABAYE3578
ABN: AB57_0288(aphA1b)
ABZ: ABZJ_05085
ABD: ABTW07_3876(aphA1b) ABTW07_3891(aphA1b)
ABJ: BJAB07104_p0106
ABAB: BJAB0715_p0002
ABAJ: BJAB0868_p0097
ABAZ: AGW27715.1
ABW: BL01_19125
ANO: RR32_19120
AJO: RZ95_17955
ASJ: AsACE_p200011
ADV: DJ533_01225 DJ533_07430
ACUM: C9E88_015545(aph(3')-VI)
AGU: AS4_23160
ACHI: CDG60_00305(aph(3')-VI) CDG60_00550
MBL: AAX09_10060
PLZ: S4054249_10315
SAGA: M5M_18870
AEA: C2U39_23255(aph(3')-II)
AES: C2U30_02870(aph(3')-I)
SOK: D0B54_04480
REV: HUE57_13035
NBL: GJV52_09465(aph(3')-IIIa)
CHIZ: HQ393_17285
REH: H16_B1333(h16_B1333)
BUR: Bcep18194_B2197
BCJ: BCAM0928(neo)
BCEO: I35_4848(neo)
BCED: DM42_4158(neo)
BCON: NL30_36050
BSTL: BBJ41_32400
PHS: C2L64_52670(aph(3')-I)
BTRM: SAMEA390648702979(neo)
AIS: BUW96_05100
ACHB: DVB37_20695
AFA: UZ73_08990
DLA: I6G47_08505
JAZ: YQ44_09405
JAL: BZG29_20520
JSV: CNX70_20685
JAS: FJQ89_10355
JLV: G3257_21175(aph(3')-I)
MASZ: C9I28_21630
MASY: DPH57_00520
MFLA: GO485_08340(aph(3')-II)
MPLI: E1742_08700
DUG: HH213_14175(aph(3')-I)
MIU: ABE85_18090
CJEN: N755_01768(aphA)
CCC: G157_08935
CCY: YSS_10115
MLO: mlr2696
MESP: C1M53_20070
AAK: AA2016_2415
AMIH: CO731_02415(neo)
SME: SMc03094
SMK: Sinme_3053
SMQ: SinmeB_2827
SMX: SM11_chr3177
SMI: BN406_02856(neo)
SMEG: C770_GR4Chr3137
SMEL: SM2011_c03094
SMER: DU99_16540
EAH: FA04_16285
ARA: Arad_3116
RET: RHE_CH02947
REC: RHECIAT_CH0003111
REL: REMIM1_CH02987
REP: IE4803_CH03173
REI: IE4771_CH03209
RLE: RL3397(neo)
RLT: Rleg2_2693
RLG: Rleg_2951
RLB: RLEG3_25360
RLU: RLEG12_24660
RTR: RTCIAT899_CH12390
RPUS: CFBP5875_03820
RGA: RGR602_CH01541
RHN: AMJ98_CH03107
RPHA: AMC79_CH03109
RHX: AMK02_CH03039
RHK: Kim5_CH03209
REZ: AMJ99_CH03106
RHR: CKA34_15270
RAD: CO657_13770
RII: FFM53_020085
RHID: FFM81_006395
NGL: RG1141_CH16900
OIN: IAR37_12815
OAN: Oant_1320
OAH: DR92_883(neo)
OPS: A8A54_08305
OCH: CES85_1994
BRK: CWS35_05175
RPT: Rpal_0195
LNE: FZC33_33600
CHEL: AL346_10300
CDQ: BOQ54_16880
DEI: C4375_17355
MAAD: AZF01_20235
PSF: PSE_4262(neo)
PPHR: APZ00_03710
CFH: C1707_08575
NOR: FA702_16840(aph(3')-I)
SPHF: DM480_00550 DM480_17500(aph(3')-I)
SECH: B18_03710
PHZ: CHX26_14640
RCE: RC1_0237(butA)
AZZ: DEW08_09705
PSTG: E8M01_04605 E8M01_04630
MEKU: HUW50_16765
SAX: pUSA300HOUMR0018
SUZ: MS7_0063(aphA)
SUW: SATW20_20760(aphA)
SAUG: SA268_2516
SAUZ: SAZ172_2091
SAUR: SABB_02281(aphA)
SAUI: AZ30_14265
SER: SEA0010(aphA)
SHH: ShL2_02167(aphA)
SDT: SPSE_1794(aphA)
SWA: A284_12042
GYM: GYMC10_0161
PALB: EJC50_17330
PBK: Back11_32840 Back11_38870(ymdC)
PLYC: GXP70_15210
LLA: L33782(ymdC)
LLK: LLKF_1253(ymdC)
LLT: CVCAS_1176(ymdC)
LLS: lilo_1100(ymdC)
LLD: P620_06885
LLX: NCDO2118_1221(ymdC)
LLM: llmg_1293
LLC: LACR_1330
LLR: llh_6710
LLN: LLNZ_06680
LLI: uc509_1219
LLW: kw2_1172
SPW: SPCG_1325 SPCG_1876
SPP: SPP_1930
SNU: SPNA45_00348
SSA: SSA_1830
SSUS: NJAUSS_0666
SUI: SSUJS14_0692
SGO: SGO_0628(ymdC)
SEZ: Sez_1628
SEQ: SZO_03450
SEZO: SeseC_02178
SEU: SEQ_1846
SMB: smi_1708(aphA)
STD: SPPN_09665
SIE: SCIM_0382
SIB: SIR_1285
SIU: SII_1309
SANG: SAIN_0411
SCG: SCI_0444
SCON: SCRE_0424
SCOS: SCR2_0424
SPEI: EHW89_01960
SPLR: C0J00_02060
LJO: LJ_1688
LJF: FI9785_1471(aph)
LJH: LJP_1424c
LJN: T285_07210
LAC: LBA1348
LAD: LA14_1348
LAF: SD55_1334
LGA: LGAS_1458
EFAU: EFAU085_p3013(aphA)
EFU: HMPREF0351_12797(aphA) HMPREF0351_12830(aphA2)
RHO: RHOM_01605
ERM: EYR00_09540
EBM: SG0102_14070(aphA)
AOC: Aocu_00810
MTU: Rv3817
MTV: RVBD_3817
MTC: MT3925.1(aph)
MRA: MRA_3857(aph)
MTF: TBFG_13851
MTB: TBMG_03864
MTK: TBSG_03887
MTZ: TBXG_003834
MTG: MRGA327_23505
MTI: MRGA423_24085
MTE: CCDC5079_3547
MTUR: CFBS_4047(aph)
MTL: CCDC5180_3495
MTO: MTCTRI2_3896
MTD: UDA_3817
MTN: ERDMAN_4184
MTJ: J112_20525
MTUB: MT7199_3886
MTUC: J113_26725
MTUE: J114_20400
MTX: M943_19600
MTUH: I917_26845
MTUL: TBHG_03755
MTUT: HKBT1_4030(aph)
MTUU: HKBT2_4040(aph)
MTQ: HKBS1_4043(aph)
MBO: BQ2027_MB3847
MBB: BCG_3879
MBT: JTY_3881
MBM: BCGMEX_3880
MBK: K60_039600
MBX: BCGT_3681
MAF: MAF_38320
MMIC: RN08_4213
MCE: MCAN_38361
MCQ: BN44_120227
MCV: BN43_90334
MCX: BN42_90342
MCZ: BN45_110179
MYO: OEM_11520
MCHI: AN480_06785 AN480_29000
MSA: Mycsm_06204
MUL: MUL_5000
MMI: MMAR_5380
MMAE: MMARE11_52010
MLI: MULP_05659
MKN: MKAN_14280
MHAD: B586_02960
MDX: BTO20_33610
MSHG: MSG_04888
MFJ: MFLOJ_36310
MPAG: C0J29_29735
MGOR: H0P51_27540
MCB: Mycch_5004
MAUU: NCTC10437_05419(neo)
MMOR: MMOR_07670
MFX: MFAL_38020
MIJ: MINS_38300
MARZ: MARA_44190
MAB: MAB_0163c
MMV: MYCMA_0096
MABB: MASS_0161
MABL: MMASJCM_0165
MKR: MKOR_40480
CUR: cu1535(aph(3')-Ia)
CUA: CU7111_1481(aph(3')-Ia)
CSX: CSING_02010(aphA1)
CCJZ: ccrud_14555
NFA: NFA_38480(aph(3'))
NCY: NOCYR_3725
NBR: O3I_030060
NAH: F5544_30275
NAD: NCTC11293_05205(neo)
TPR: Tpau_1464
DIZ: CT688_13520
SBH: SBI_05609
SRN: A4G23_05133(neo)
SALF: SMD44_p10101
SRIM: CP984_09245(aph(3')-VIII)
KSK: KSE_41510
MTS: MTES_1899
MIO: AOA12_18300
MIP: AXH82_04640
MCW: A8L33_09395
MPAL: BO218_08770
MAUR: BOH66_01830
MFOL: DXT68_15175
MOO: BWL13_02576(neo)
MWA: E4K62_17060
MPRT: ET475_03850
MOY: CVS54_03366(neo)
MSF: IT882_06765
CUB: BJK06_05580
FRP: AX769_02465
AGY: ATC03_08620
AGX: AGREI_1209
ARL: AFL94_01545 AFL94_17275
ARY: ATC04_16470
AAI: AARI_03100
GAR: AOZ07_01940
KRH: KRH_10490
XYL: ET495_07705
CET: B8281_09980
CCEU: CBR64_01135
XYA: ET471_00320
SANW: G7063_05425
CFL: Cfla_1184
CFI: Celf_1130
CGA: Celgi_0943
CEZ: CBP52_08815
CELZ: E5225_12125
CEJ: GC089_12660
CELH: GXP71_10685
OEK: FFI11_009870
PBO: PACID_10930
AACI: ASQ49_15725
TDF: H9L22_03570
TLA: TLA_TLA_02719(neo)
PRV: G7070_05975
KFL: Kfla_1280
KQI: F1D05_25900
NDA: Ndas_3531
NAL: B005_4376
SRO: Sros_0454 Sros_5038
FRA: Francci3_3368
KRA: Krad_1799
SACC: EYD13_01980(neo)
AMI: Amir_1990
LED: BBK82_29490
AHM: TL08_05855
MTUA: CSH63_05730
MSAG: GCM10017556_22720
AFS: AFR_29645
PLAB: C6361_07070
PLAT: C6W10_03645
CAI: Caci_6179
BBB: BIF_01665
BNM: BALAC2494_01072
BLW: W7Y_0046
BLS: W91_0045
BNI: BANAN_00270
BANM: EN10_00245
BBP: BBPR_1808
BBI: BBIF_1747
BBF: BBB_1805(ymdC)
BPSP: AH67_00170
BSCA: BBSC_0104
BCHO: BcFMB_02555
BLEM: BL8807_09340
BEU: BE0216_02055
ATQ: GH723_07245(aph(3'))
GEI: GEI7407_2800
CAU: Caur_2511
CHL: Chy400_2709
KBS: EPA93_24010
DRA: DR_0066
DGE: Dgeo_2256
DDR: Deide_21650
DMR: Deima_0344
DPT: Deipr_1927
DGO: DGo_CA0345
DPD: Deipe_2720
DSW: QR90_03680
DCH: SY84_07575
DAB: AUC44_14545
DPU: SU48_00375
DEZ: DKM44_13465
DWU: DVJ83_11460
DFC: DFI_01070
DEIN: DAAJ005_14275
DGA: DEGR_29090
MSV: Mesil_0966
AHEL: Q31a_41240
BUY: D8S85_06000(aph(3')-IIIa)
 » show all
Reference
1  [PMID:4970990]
  Authors
Doi O, Ogura M, Tanaka N, Umezawa H.
  Title
Inactivation of kanamycin, neomycin, and streptomycin by enzymes obtained in cells of Pseudomonas aeruginoa.
  Journal
Appl Microbiol 16:1276-81 (1968)
Reference
2  [PMID:13416259]
  Authors
DOLIN MI.
  Title
The Streptococcus faecalis oxidases for reduced diphosphopyridine nucleotide.  III.  Isolation and properties of a flavin peroxidase for reduced diphosphopyridine nucleotide.
  Journal
J Biol Chem 225:557-73 (1957)
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.95
IUBMB Enzyme Nomenclature: 2.7.1.95
ExPASy - ENZYME nomenclature database: 2.7.1.95
BRENDA, the Enzyme Database: 2.7.1.95
CAS: 62213-36-9

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Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (3)   
   KEGG REACTION (1)   
   KEGG RCLASS (2)   
Gene (793)   
   KEGG ORTHOLOGY (7)   
   KEGG GENES (463)   
   KEGG MGENES (291)   
   RefGene (32)   
Protein sequence (2456)   
   UniProt (2367)   
   SWISS-PROT (12)   
   RefSeq(pep) (4)   
   PDBSTR (68)   
   PMD (5)   
DNA sequence (1341)   
   RefSeq(nuc) (75)   
   GenBank (676)   
   EMBL (590)   
3D Structure (17)   
   PDB (17)   
Protein domain (3)   
   InterPro (3)   
All databases (4617)   

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