KEGG   ENZYME: 2.7.1.95
Entry
EC 2.7.1.95                 Enzyme                                 
Name
kanamycin kinase;
neomycin-kanamycin phosphotransferase
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
Sysname
ATP:kanamycin 3'-O-phosphotransferase
Reaction(IUBMB)
ATP + kanamycin = ADP + kanamycin 3'-phosphate
Reaction(KEGG)
(other) R01888
Substrate
ATP [CPD:C00002];
kanamycin [CPD:C00304]
Product
ADP [CPD:C00008];
kanamycin 3'-phosphate [CPD:C03281]
Comment
Also acts on the antibiotics neomycin, paromomycin, neamine, paromamine, vistamycin and gentamicin A. An enzyme from Pseudomonas aeruginosa also acts on butirosin.
History
EC 2.7.1.95 created 1976
Orthology
K00897  kanamycin kinase
K19272  aminoglycoside 3'-phosphotransferase I
K19274  aminoglycoside 3'-phosphotransferase VI
K19299  aminoglycoside 3'-phosphotransferase III
K19300  aminoglycoside 3'-phosphotransferase II
K19315  aminoglycoside 3'-phosphotransferase XV
K19543  aminoglycoside 3'-phosphotransferase
Genes
MPHA105840509
EHIEHI_117050
EBWBWG_3698
EOIECO111_p1-120
EOJECO26_p2-04
ECMEcSMS35_A0159
SECSCH_026(aph)
ECLEECNIH2_23600 ECNIH2_26045
ECLYLI62_013010 LI62_11650
KPNKPN_pKPN5p08194(aphA)
KPZKPNIH27_25655
KPGKPNIH32_28420
KPCKPNIH10_26765
KPBFH42_25875
KPNEKU54_00025
KPNULI86_26895
KOXKOX_20540
KOEA225_2977
KOYJ415_17065
KOMHR38_18875
KOKKONIH1_14625 KONIH1_30465
KOCAB185_22765
CFDCFNIH1_24930
CWECO701_09595
CPARCUC49_08460
CITZE4Z61_00180
KIENCTC12125_04789(neo)
KINAB182_03320
SMAFD781_0520
SRZAXX16_0005
SERMCLM71_02525
SRHZFO014_07330
PANPPSNIH2_20340
PCOLF1325_16150(aph(3')-I)
PCIBF9282_16450(aph(3')-VI)
PSTABGK56_21235
MWIMNY66_17570
PMPPmu_02740(aphA1)
MHXMHH_c22550(aphA1)
AIOEXH44_00925
GANUMN179_01162
BTOWQG_1070
BTRHF543_22790
BTRAF544_3130
SMLSmlt2120
SMTSmal_1717
BUJBurJV3_1756
SMZSMD_1909
STENCCR98_09260
STEMCLM74_09685
STESMG068_09120(aph(3')-II)
SINCDAIF1_18880(neo)
SPAQSTNY_R18290(aph(3')-II)
LMBC9I47_1673
LUEDCD74_02565(aph(3')-XV)
DKOI596_3655
PAEPA4119(aph)
PAEVN297_4251(neo)
PAEIN296_4251(neo)
PAUPA14_10670(aph)
PAPPSPA7_0973(aph) PSPA7_3721
PAGPLES_08521(aph)
PNCNCGM2_5320(aph)
PAEBNCGM1900_0841(aph)
PSGG655_04105
PRPM062_21725
PAEPPA1S_04225
PAERPA1R_gp2010
PAEMU769_04250
PAELT223_04160
PAESSCV20265_0848
PAEUBN889_04573(aph)
PAEGAI22_29390
PAECM802_4249(neo)
PAEOM801_4117(neo)
PPUHB479_26894
PFBVO64_1917
PTVAA957_19685
PFWPF1751_v1c45260
PFFPFLUOLIPICF713405
PSILPMA3_18220
PYMAK972_1650
PUMHGP31_03940
PGYAWU82_21750(aph(3')-I)
PSHHHU773_006205
PMUYKSS95_22410
PJUL1P09_08635 L1P09_25445(aph(3')-XV)
PSTUUIB01_10035
PCHLLLJ08_09385
SDEGGOM96_02975(aph(3')-II)
ABYABAYE3578
ABNAB57_0288(aphA1b)
ABZABZJ_05085
ABDABTW07_3876(aphA1b) ABTW07_3891(aphA1b)
ABJBJAB07104_p0106
ABABBJAB0715_p0002
ABAJBJAB0868_p0097
ABAZAGW27715.1
ABWBL01_19125
ANORR32_19120
AJORZ95_17955
ASJAsACE_p200011
ADVDJ533_01225 DJ533_07430
ACUMC9E88_015545(aph(3')-VI)
AGUAS4_23160
ACHICDG60_00305(aph(3')-VI) CDG60_00550
ATGJ4G44_12780
AVZHWI77_18555(aph(3')-XV)
MBLAAX09_10060
PLZS4054249_10315
SAGAM5M_18870
AEAC2U39_23255(aph(3')-II)
AESC2U30_02870(aph(3')-I)
SOKD0B54_04480
NBLGJV52_09465(aph(3')-IIIa)
CHIZHQ393_17285
REHH16_B1333(h16_B1333)
BURBcep18194_B2197
BCJBCAM0928(neo)
BCEOI35_4848(neo)
BCEDDM42_4158(neo)
BCONNL30_36050
BSTLBBJ41_32400
PHSC2L64_52670(aph(3')-I)
BTRMSAMEA390648702979(neo)
AISBUW96_05100
ACHBDVB37_20695
ADYHLG70_04670
AFAUZ73_08990
DLAI6G47_08505
MIUABE85_18090
JAZYQ44_09405
JALBZG29_20520
JSVCNX70_20685
JASFJQ89_10355
JLVG3257_21175(aph(3')-I)
MASYDPH57_00520
MFYHH212_05875
MASZC9I28_21630
MFLAGO485_08340(aph(3')-II)
MPLIE1742_08700
DUGHH213_14175(aph(3')-I)
CJENN755_01768(aphA)
CCCG157_08935
CCYYSS_10115
MLOmlr2696
MESPC1M53_20070
AAKAA2016_2415
AMIHCO731_02415(neo)
SMESMc03094
SMKSinme_3053
SMQSinmeB_2827
SMXSM11_chr3177
SMIBN406_02856(neo)
SMEGC770_GR4Chr3137
SMELSM2011_c03094
SMERDU99_16540
EAHFA04_16285
ARAArad_3116
RETRHE_CH02947
RECRHECIAT_CH0003111
RELREMIM1_CH02987
REPIE4803_CH03173
REIIE4771_CH03209
RLERL3397(neo)
RLTRleg2_2693
RLGRleg_2951
RLBRLEG3_25360
RLURLEG12_24660
RTRRTCIAT899_CH12390
RPUSCFBP5875_03820
RGARGR602_CH01541
RHNAMJ98_CH03107
RPHAAMC79_CH03109
RHXAMK02_CH03039
RHKKim5_CH03209
REZAMJ99_CH03106
RHRCKA34_15270
RADCO657_13770
RIIFFM53_020085
RHIDFFM81_006395
RBQJ2J99_15455
RLNJ0663_02250
RRGJ3P73_17515
NGLRG1141_CH16900
OINIAR37_12815
OANOant_1320
OAHDR92_883(neo)
OPSA8A54_08305
OCHCES85_1994
OCRHGK82_09445
OCLGTN27_09755(aph(3'))
BRKCWS35_05175
RPTRpal_0195
LNEFZC33_33600
CHELAL346_10300
CDQBOQ54_16880
DEIC4375_17355
DSALK1X15_08780
DEVOH4N61_08845
MAADAZF01_20235
PSFPSE_4262(neo)
PPHRAPZ00_03710
PSTGE8M01_04605 E8M01_04630
CFHC1707_08575
BNDKWG56_14220(aph(3')-XV)
NORFA702_16840(aph(3')-I)
SPHFDM480_00550 DM480_17500(aph(3')-I)
SECHB18_03710
SCHYGVO57_08575(aph(3'))
SBINSBA_ch1_03620
PHZCHX26_14640
AZZDEW08_09705
RCERC1_0237(butA)
MEKUHUW50_16765
BOUI5818_13625 I5818_13630
SAXpUSA300HOUMR0018
SUZMS7_0063(aphA)
SUWSATW20_20760(aphA)
SAUGSA268_2516
SAUZSAZ172_2091
SAURSABB_02281(aphA)
SAUIAZ30_14265
SERSEA0010(aphA)
SHHShL2_02167(aphA)
SDTSPSE_1794(aphA)
SWAA284_12042
GYMGYMC10_0161
PALBEJC50_17330
PBKBack11_32840 Back11_38870(ymdC)
PLYCGXP70_15210
LLAL33782(ymdC)
LLKLLKF_1253(ymdC)
LLTCVCAS_1176(ymdC)
LLSlilo_1100(ymdC)
LLDP620_06885
LLXNCDO2118_1221(ymdC)
LLMllmg_1293
LLCLACR_1330
LLRllh_6710
LLNLLNZ_06680
LLIuc509_1219
LLWkw2_1172
SPWSPCG_1325 SPCG_1876
SPPSPP_1930
SNUSPNA45_00348
SSASSA_1830
SSUSNJAUSS_0666
SUISSUJS14_0692
SGOSGO_0628(ymdC)
SEZSez_1628
SEQSZO_03450
SEZOSeseC_02178
SEUSEQ_1846
SMBsmi_1708(aphA)
STDSPPN_09665
SIESCIM_0382
SIBSIR_1285
SIUSII_1309
SANGSAIN_0411
SCGSCI_0444
SCONSCRE_0424
SCOSSCR2_0424
STRNSNAG_0434(neo)
SPEIEHW89_01960
SPLRC0J00_02060
SMENSAMEA4412692_1588(butA) SAMEA4412692_1589(ymdC)
SLATJ4854_02790
SACOSAME_00507(ymdC)
LJOLJ_1688
LJFFI9785_1471(aph)
LJHLJP_1424c
LJNT285_07210
LACLBA1348
LADLA14_1348
LAFSD55_1334
LGALGAS_1458
LTAH1A07_07220
EFAUEFAU085_p3013(aphA)
EFUHMPREF0351_12797(aphA) HMPREF0351_12830(aphA2)
ERAFJ9537_20510(aph(3')-IIIa)
SMANC12CBH8_05220(ymdC)
RHORHOM_01605
ERMEYR00_09540
EBMSG0102_14070(aphA)
AOCAocu_00810
MTURv3817
MTVRVBD_3817
MTCMT3925.1(aph)
MRAMRA_3857(aph)
MTFTBFG_13851
MTBTBMG_03864
MTKTBSG_03887
MTZTBXG_003834
MTGMRGA327_23505
MTIMRGA423_24085
MTECCDC5079_3547
MTURCFBS_4047(aph)
MTLCCDC5180_3495
MTOMTCTRI2_3896
MTDUDA_3817
MTNERDMAN_4184
MTJJ112_20525
MTUBMT7199_3886
MTUCJ113_26725
MTUEJ114_20400
MTXM943_19600
MTUHI917_26845
MTULTBHG_03755
MTUTHKBT1_4030(aph)
MTUUHKBT2_4040(aph)
MTQHKBS1_4043(aph)
MBOBQ2027_MB3847
MBBBCG_3879
MBTJTY_3881
MBMBCGMEX_3880
MBKK60_039600
MBXBCGT_3681
MAFMAF_38320
MMICRN08_4213
MCEMCAN_38361
MCQBN44_120227
MCVBN43_90334
MCXBN42_90342
MCZBN45_110179
MYOOEM_11520
MCHIAN480_06785 AN480_29000
MSAMycsm_06204
MULMUL_5000
MMIMMAR_5380
MMAEMMARE11_52010
MLIMULP_05659
MKNMKAN_14280
MHADB586_02960
MDXBTO20_33610
MSHGMSG_04888
MFJMFLOJ_36310
MSTOMSTO_40680
MKUI2456_27545
MPAGC0J29_29735
MGORH0P51_27540
MBAIMB901379_04821(neo)
MPSEMPSD_55070
MSHOMSHO_08310
MLJMLAC_44020
MSHJMSHI_34300
MKRMKOR_40480
MSPGF6B93_22015
MOTLTS72_25720
MVMMJO54_07505
MRFMJO55_26025
MCBMycch_5004
MAUUNCTC10437_05419(neo)
MMORMMOR_07670
MFXMFAL_38020
MIJMINS_38300
MARZMARA_44190
MAUBMAUB_30310
MPHUMPHO_39340
MMUCC1S78_028100
MMATMMAGJ_28210
MSEIMSEDJ_06470
MFLVNCTC10271_00197(neo)
MCEEMCEL_34050
MABMAB_0163c
MMVMYCMA_0096
MABBMASS_0161
MABLMMASJCM_0165
CURcu1535(aph(3')-Ia)
CUACU7111_1481(aph(3')-Ia)
CSXCSING_02010(aphA1)
CCJZccrud_14555
CGLUI6J20_10650 I6J20_10660
CWKIA203_04670
NFANFA_38480(aph(3'))
NCYNOCYR_3725
NBRO3I_030060
NAHF5544_30275
NADNCTC11293_05205(neo)
NIEKV110_29075
NHUH0264_30970
NGPLTT66_24220
GOILK459_00010
TPRTpau_1464
DIZCT688_13520
SBHSBI_05609
SRNA4G23_05133(neo)
SRIMCP984_09245(aph(3')-VIII)
SFBCP974_27395
SROIIAG44_36080
SXNIAG42_31220
SHKJ2N69_01555
KSKKSE_41510
YIALO772_33025
MTSMTES_1899
MIOAOA12_18300
MIPAXH82_04640
MCWA8L33_09395
MPALBO218_08770
MAURBOH66_01830
MFOLDXT68_15175
MOOBWL13_02576(neo)
MWAE4K62_17060
MPRTET475_03850
MOYCVS54_03366(neo)
MSFIT882_06765
MLZF6J85_16065
MCHNHCR76_02755
MICSC1N74_02210
CUBBJK06_05580
FRPAX769_02465
AGYATC03_08620
ASOIMTP13_12970
LVIG7068_05495
LLUTK1X41_02650
AGXAGREI_1209
ARLAFL94_01545 AFL94_17275
ARYATC04_16470
AAIAARI_03100
GARAOZ07_01940
GLUF0M17_01505
GPRJQN66_01610
GMINMP99_01805
GMYXH9_07065
KRHKRH_10490
XYLET495_07705
XYAET471_00320
CETB8281_09980
CCEUCBR64_01135
SANWG7063_05425
CFLCfla_1184
CFICelf_1130
CGACelgi_0943
CEZCBP52_08815
CELZE5225_12125
CEJGC089_12660
CELHGXP71_10685
CIRALFM56_12285
CPALF1D97_15530
OEKFFI11_009870
PBOPACID_10930
AACIASQ49_15725
TDFH9L22_03570
TLATLA_TLA_02719(neo)
PRVG7070_05975
NPCKUV85_00315
KFLKfla_1280
KQIF1D05_25900
THAONI17_007520
TALXFOF52_05830
NDANdas_3531
NALB005_4376
SAIUJ4H86_03160
SROSros_0454 Sros_5038
FRAFrancci3_3368
KRAKrad_1799
SACCEYD13_01980(neo)
AMIAmir_1990
LEDBBK82_29490
AHMTL08_05855
MTUACSH63_05730
MSAGGCM10017556_22720
AFSAFR_29645
PLABC6361_07070
PLATC6W10_03645
PRYPrubr_49580
DVCDvina_05435
CAICaci_6179
GLYK3N28_18390
STUCG5S47_08045
BBBBIF_01665
BNMBALAC2494_01072
BLWW7Y_0046
BLSW91_0045
BNIBANAN_00270
BANMEN10_00245
BBPBBPR_1808
BBIBBIF_1747
BBFBBB_1805(ymdC)
BPSPAH67_00170
BSCABBSC_0104
BCHOBcFMB_02555
BLEMBL8807_09340
BEUBE0216_02055
JTLM6D93_06060
ATQGH723_07245(aph(3'))
EGDGS424_013585
GEIGEI7407_2800
CAUCaur_2511
CHLChy400_2709
KBSEPA93_24010
PMETG4Y79_23865
DRADR_0066
DGEDgeo_2256
DDRDeide_21650
DMRDeima_0344
DPTDeipr_1927
DGODGo_CA0345
DPDDeipe_2720
DSWQR90_03680
DCHSY84_07575
DABAUC44_14545
DPUSU48_00375
DEZDKM44_13465
DWUDVJ83_11460
DFCDFI_01070
DEINDAAJ005_14275
DGADEGR_29090
DPHEHF33_12990
DRDLMT64_09225
MSVMesil_0966
RUFIK0V07_16440
CVLJ8C06_13920
PGOLK6V26_03545
BUYD8S85_06000(aph(3')-IIIa)
COYHF329_05125
SRHOHH216_17180
 » show all
Reference
1  [PMID:4970990]
  Authors
Doi O, Ogura M, Tanaka N, Umezawa H.
  Title
Inactivation of kanamycin, neomycin, and streptomycin by enzymes obtained in cells of Pseudomonas aeruginoa.
  Journal
Appl Microbiol 16:1276-81 (1968)
Reference
2  [PMID:13416259]
  Authors
DOLIN MI.
  Title
The Streptococcus faecalis oxidases for reduced diphosphopyridine nucleotide.  III.  Isolation and properties of a flavin peroxidase for reduced diphosphopyridine nucleotide.
  Journal
J Biol Chem 225:557-73 (1957)
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.95
IUBMB Enzyme Nomenclature: 2.7.1.95
ExPASy - ENZYME nomenclature database: 2.7.1.95
BRENDA, the Enzyme Database: 2.7.1.95
CAS: 62213-36-9

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