KEGG   ENZYME: 2.7.1.95
Entry
EC 2.7.1.95                 Enzyme                                 

Name
kanamycin kinase;
neomycin-kanamycin phosphotransferase
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
Sysname
ATP:kanamycin 3'-O-phosphotransferase
Reaction(IUBMB)
ATP + kanamycin = ADP + kanamycin 3'-phosphate
Reaction(KEGG)
(other) R01888
Substrate
ATP [CPD:C00002];
kanamycin [CPD:C00304]
Product
ADP [CPD:C00008];
kanamycin 3'-phosphate [CPD:C03281]
Comment
Also acts on the antibiotics neomycin, paromomycin, neamine, paromamine, vistamycin and gentamicin A. An enzyme from Pseudomonas aeruginosa also acts on butirosin.
History
EC 2.7.1.95 created 1976
Orthology
K00897  kanamycin kinase
K19272  aminoglycoside 3'-phosphotransferase I
K19274  aminoglycoside 3'-phosphotransferase VI
K19299  aminoglycoside 3'-phosphotransferase III
K19300  aminoglycoside 3'-phosphotransferase II
K19315  aminoglycoside 3'-phosphotransferase XV
K19543  aminoglycoside 3'-phosphotransferase
Genes
MPHA: 105840509
EHI: EHI_117050
EBW: BWG_3698
EOI: ECO111_p1-120
EOJ: ECO26_p2-04
ECM: EcSMS35_A0159
SEC: SCH_026(aph)
ECLE: ECNIH2_23600 ECNIH2_26045
ECLY: LI62_013010 LI62_11650
KPN: KPN_pKPN5p08194(aphA)
KPNE: KU54_00025
KPNU: LI86_26895
KOX: KOX_20540
KOE: A225_2977
KIE: NCTC12125_04789(neo)
SMAF: D781_0520
PCOL: F1325_16150(aph(3')-I)
PCIB: F9282_16450(aph(3')-VI)
PMP: Pmu_02740(aphA1)
MHX: MHH_c22550(aphA1)
BTO: WQG_1070
BTRH: F543_22790
BTRA: F544_3130
SML: Smlt2120
SMT: Smal_1717
SMZ: SMD_1909
STES: MG068_09120(aph(3')-II)
SINC: DAIF1_18880(neo)
SPAQ: STNY_R18290(aph(3')-II)
LUE: DCD74_02565(aph(3')-XV)
DKO: I596_3655
PAE: PA4119(aph)
PAEV: N297_4251(neo)
PAEI: N296_4251(neo)
PAU: PA14_10670(aph)
PAG: PLES_08521(aph)
PNC: NCGM2_5320(aph)
PAEB: NCGM1900_0841(aph)
PAEP: PA1S_04225
PAEM: U769_04250
PAEL: T223_04160
PAEU: BN889_04573(aph)
PAEG: AI22_29390
PAEC: M802_4249(neo)
PAEO: M801_4117(neo)
PPUH: B479_26894
PFB: VO64_1917
PSIL: PMA3_18220
PGY: AWU82_21750(aph(3')-I)
PSEP: C4K39_5392
ABY: ABAYE3578
ABN: AB57_0288(aphA1b)
ABD: ABTW07_3876(aphA1b) ABTW07_3891(aphA1b)
ABAZ: AGW27715.1
ACUM: C9E88_015545(aph(3')-VI)
AGU: AS4_23160
ACHI: CDG60_00305(aph(3')-VI) CDG60_00550
SAGA: M5M_18870
AEA: C2U39_23255(aph(3')-II)
AES: C2U30_02870(aph(3')-I)
NBL: GJV52_09465(aph(3')-IIIa)
REH: H16_B1333(h16_B1333)
BCJ: BCAM0928(neo)
BCEO: I35_4848(neo)
BCED: DM42_4158(neo)
BCON: NL30_36050
PHS: C2L64_52670(aph(3')-I)
BTRM: SAMEA390648702979(neo)
JLV: G3257_21175(aph(3')-I)
MFLA: GO485_08340(aph(3')-II)
DUG: HH213_14175(aph(3')-I)
CJEN: N755_01768(aphA)
CCY: YSS_10115
MLO: mlr2696
AMIH: CO731_02415(neo)
SME: SMc03094
SMI: BN406_02856(neo)
SMER: DU99_16540
ARA: Arad_3116
RLE: RL3397(neo)
RLG: Rleg_2951
OAN: Oant_1320
OAH: DR92_883(neo)
OCL: GTN27_09755(aph(3'))
RPT: Rpal_0195
PSF: PSE_4262(neo)
BND: KWG56_14220(aph(3')-XV)
NOR: FA702_16840(aph(3')-I)
SPHF: DM480_00550 DM480_17500(aph(3')-I)
SECH: B18_03710
RCE: RC1_0237(butA)
SUZ: MS7_0063(aphA)
SUW: SATW20_20760(aphA)
SAUG: SA268_2516
SAUR: SABB_02281(aphA)
SAUI: AZ30_14265
SER: SEA0010(aphA)
SHH: ShL2_02167(aphA)
SDT: SPSE_1794(aphA)
LLA: L33782(ymdC)
LLK: LLKF_1253(ymdC)
LLT: CVCAS_1176(ymdC)
LLS: lilo_1100(ymdC)
LLX: NCDO2118_1221(ymdC)
LLM: llmg_1293
LLC: LACR_1330
LLR: llh_6710
LLW: kw2_1172
SPP: SPP_1930
SSA: SSA_1830
SGO: SGO_0628(ymdC)
SEZ: Sez_1628
SEQ: SZO_03450
SEU: SEQ_1846
SMB: smi_1708(aphA)
SIE: SCIM_0382
SIB: SIR_1285
SIU: SII_1309
SANG: SAIN_0411
SCG: SCI_0444
SCON: SCRE_0424
SCOS: SCR2_0424
LJO: LJ_1688
LJF: FI9785_1471(aph)
LJH: LJP_1424c
LAC: LBA1348
LAD: LA14_1348
LAF: SD55_1334
LGA: LGAS_1458
EFAU: EFAU085_p3013(aphA)
ERAF: J9537_20510(aph(3')-IIIa)
SMAN: C12CBH8_05220(ymdC)
EBM: SG0102_14070(aphA)
MTU: Rv3817
MTV: RVBD_3817
MTC: MT3925.1(aph)
MRA: MRA_3857(aph)
MTUR: CFBS_4047(aph)
MTD: UDA_3817
MTUC: J113_26725
MTUE: J114_20400
MTUH: I917_26845
MTUL: TBHG_03755
MTUT: HKBT1_4030(aph)
MTUU: HKBT2_4040(aph)
MTQ: HKBS1_4043(aph)
MBB: BCG_3879
MBT: JTY_3881
MBX: BCGT_3681
MAF: MAF_38320
MMIC: RN08_4213
MYO: OEM_11520
MUL: MUL_5000
MMI: MMAR_5380
MHAD: B586_02960
MSHG: MSG_04888
MSTO: MSTO_40680
MBAI: MB901379_04821(neo)
MPSE: MPSD_55070
MSHO: MSHO_08310
MSHJ: MSHI_34300
MAUU: NCTC10437_05419(neo)
MMOR: MMOR_07670
MARZ: MARA_44190
MAUB: MAUB_30310
MPHU: MPHO_39340
MAB: MAB_0163c
MABB: MASS_0161
CUR: cu1535(aph(3')-Ia)
CUA: CU7111_1481(aph(3')-Ia)
CSX: CSING_02010(aphA1)
NFA: NFA_38480(aph(3'))
NAD: NCTC11293_05205(neo)
TPR: Tpau_1464
SBH: SBI_05609
SRN: A4G23_05133(neo)
SRIM: CP984_09245(aph(3')-VIII)
KSK: KSE_41510
MTS: MTES_1899
MOO: BWL13_02576(neo)
MOY: CVS54_03366(neo)
KRH: KRH_10490
CFL: Cfla_1184
CFI: Celf_1130
TLA: TLA_TLA_02719(neo)
KFL: Kfla_1280
NDA: Ndas_3531
NAL: B005_4376
KRA: Krad_1799
SACC: EYD13_01980(neo)
AMI: Amir_1990
AFS: AFR_29645
CAI: Caci_6179
BBB: BIF_01665
BLW: W7Y_0046
BLS: W91_0045
BANM: EN10_00245
BBP: BBPR_1808
BBI: BBIF_1747
BBF: BBB_1805(ymdC)
BPSP: AH67_00170
BSCA: BBSC_0104
ATQ: GH723_07245(aph(3'))
CAU: Caur_2511
DRA: DR_0066
DGE: Dgeo_2256
DFC: DFI_01070
BUY: D8S85_06000(aph(3')-IIIa)
 » show all
Reference
1  [PMID:4970990]
  Authors
Doi O, Ogura M, Tanaka N, Umezawa H.
  Title
Inactivation of kanamycin, neomycin, and streptomycin by enzymes obtained in cells of Pseudomonas aeruginoa.
  Journal
Appl Microbiol 16:1276-81 (1968)
Reference
2  [PMID:13416259]
  Authors
DOLIN MI.
  Title
The Streptococcus faecalis oxidases for reduced diphosphopyridine nucleotide.  III.  Isolation and properties of a flavin peroxidase for reduced diphosphopyridine nucleotide.
  Journal
J Biol Chem 225:557-73 (1957)
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.95
IUBMB Enzyme Nomenclature: 2.7.1.95
ExPASy - ENZYME nomenclature database: 2.7.1.95
BRENDA, the Enzyme Database: 2.7.1.95
CAS: 62213-36-9

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