KEGG   ENZYME: 2.7.11.18
Entry
EC 2.7.11.18                Enzyme                                 

Name
myosin-light-chain kinase;
[myosin-light-chain] kinase;
ATP:myosin-light-chain O-phosphotransferase;
calcium/calmodulin-dependent myosin light chain kinase;
MLCK;
MLCKase;
myosin kinase;
myosin light chain kinase;
myosin light chain protein kinase;
myosin light-chain kinase (phosphorylating);
smooth-muscle-myosin-light-chain kinase;
STK18
Class
Transferases;
Transferring phosphorus-containing groups;
Protein-serine/threonine kinases
Sysname
ATP:[myosin light chain] O-phosphotransferase
Reaction(IUBMB)
ATP + [myosin light chain] = ADP + [myosin light chain] phosphate [RN:R03150]
Reaction(KEGG)
R03150
Substrate
ATP [CPD:C00002];
myosin light chain [CPD:C01003]
Product
ADP [CPD:C00008];
myosin light chain phosphate [CPD:C03875]
Comment
Requires Ca2+ and calmodulin for activity. The 20-kDa light chain from smooth muscle myosin is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors, but more slowly.
History
EC 2.7.11.18 created 1986 as EC 2.7.1.117, transferred 2005 to EC 2.7.11.18
Orthology
K00907  myosin-light-chain kinase
Genes
HSA: 340156(MYLK4) 4638(MYLK) 85366(MYLK2) 91807(MYLK3)
PTR: 101057233(MYLK) 465143(MYLK3) 471840(MYLK4) 742591(MYLK2)
PPS: 100974667(MYLK3) 100978891(MYLK2) 100979422(MYLK) 100995666(MYLK4)
GGO: 101142619(MYLK3) 101143745(MYLK2) 101151517(MYLK) 101151667(MYLK4)
PON: 100171951(MYLK3) 100444714(MYLK4) 100452913(MYLK) 100453783(MYLK2)
NLE: 100585981(MYLK2) 100591151(MYLK) 100594745(MYLK4) 100604194 115831086(MYLK3)
MCC: 707361(MYLK4) 712830(MYLK2) 715422(MYLK) 716132(MYLK3)
MCF: 102122026(MYLK4) 102130711(MYLK) 102132754(MYLK3) 102145766(MYLK2)
CSAB: 103222295(MYLK4) 103228167(MYLK) 103232215(MYLK3) 103248051(MYLK2)
CATY: 105582238(MYLK2) 105589245(MYLK3) 105589789(MYLK) 105596420(MYLK4)
PANU: 101007177(MYLK4) 101012839(MYLK3) 101016979(MYLK) 101018714(MYLK2)
RRO: 104653717(MYLK2) 104655407(MYLK) 104655430(MYLK3) 104661238(MYLK4)
RBB: 108514260(MYLK4) 108517416(MYLK) 108524142(MYLK2) 108534775(MYLK3)
TFN: 117068620(MYLK2) 117083396(MYLK) 117086614(MYLK4) 117087736(MYLK3)
PTEH: 111524418(MYLK3) 111533516 111548207(MYLK2) 111548560(MYLK4)
CJC: 100391801(MYLK2) 100392653(MYLK3) 100392656(MYLK4) 100409440(MYLK)
SBQ: 101029220(MYLK) 101038668(MYLK2) 101049056(MYLK4) 101050910(MYLK3)
MMUR: 105866079(MYLK2) 105873493(MYLK4) 105876221(MYLK3) 105879866(MYLK)
MMU: 107589(Mylk) 213435(Mylk3) 228785(Mylk2) 238564(Mylk4)
MCAL: 110290127(Mylk2) 110300419(Mylk3) 110308670(Mylk4) 110311174(Mylk)
MPAH: 110319274(Mylk2) 110329346(Mylk) 110334129(Mylk4) 110337577(Mylk3)
RNO: 100911165(Mylk4) 117558(Mylk2) 288057(Mylk) 291926(Mylk3)
MCOC: 116069762(Mylk3) 116081489(Mylk4) 116085852(Mylk) 116091182(Mylk2)
MUN: 110547228(Mylk) 110550095(Mylk2) 110552995(Mylk3) 110558635(Mylk4)
CGE: 100755963(Mylk2) 100756400(Mylk4) 100758406(Mylk) 100766446(Mylk3)
PLEU: 114702188(Mylk4) 114704843(Mylk) 114705574(Mylk2) 114709967(Mylk3)
NGI: 103731078(Mylk2) 103737011(Mylk4) 103741992(Mylk)
HGL: 101701713(Mylk4) 101704236(Mylk) 101712444(Mylk2)
CPOC: 100721558(Mylk4) 100724875(Mylk2) 100728232(Mylk3)
CCAN: 109677703 109691764(Mylk2) 109700226(Mylk4)
OCU: 100009051(MYLK2) 100348694(MYLK3) 100353972(MYLK4)
OPI: 101517477(MYLK3) 101526040(MYLK) 101526659(MYLK2) 101526925(MYLK4)
TUP: 102468205(MYLK3) 102476504(MYLK) 102489117(MYLK2) 102498032(MYLK4)
CFA: 475345(MYLK3) 477187(MYLK2) 488012(MYLK) 488190
VVP: 112907401(MYLK4) 112909931(MYLK2) 112913506(MYLK) 112914947(MYLK3)
VLG: 121478392(MYLK2) 121481492(MYLK3) 121499771(MYLK4) 121500652(MYLK)
AML: 100469244(MYLK2) 100473453(MYLK4) 100474254(MYLK3) 100481263(MYLK)
UMR: 103665161(MYLK4) 103673818(MYLK3) 103674343(MYLK2) 103678824(MYLK)
UAH: 113252202(MYLK3) 113252350(MYLK) 113258196(MYLK2) 113264229(MYLK4)
ORO: 101368074(MYLK4) 101370711(MYLK3) 101378973(MYLK) 101381609(MYLK2)
MPUF: 101673442(MYLK3) 101673644(MYLK2) 101681427(MYLK4)
EJU: 114199360(MYLK3) 114199417(MYLK) 114220792(MYLK2) 114223891(MYLK4)
MLX: 118007012(MYLK) 118011350(MYLK2) 118023826(MYLK3) 118027388(MYLK4)
FCA: 101088408(MYLK4) 101091744(MYLK3) 101095813(MYLK2)
PYU: 121012339(MYLK2) 121020417(MYLK4) 121036344(MYLK) 121045237(MYLK3)
PBG: 122490426(MYLK4) 122491527(MYLK) 122495091(MYLK3) 122496404(MYLK2)
PTG: 102951994(MYLK2) 102962607(MYLK3) 102968305(MYLK4) 102972928(MYLK)
PPAD: 109249481(MYLK4) 109266499(MYLK) 109270987(MYLK3) 109272590(MYLK2)
AJU: 106965987(MYLK3) 106976155(MYLK4) 106984088(MYLK) 106986013(MYLK2)
HHV: 120235452(MYLK2) 120237364(MYLK) 120238194(MYLK3) 120241448(MYLK4)
BTA: 338037(MYLK) 518025(MYLK4) 526379(MYLK3) 533378(MYLK2)
BOM: 102264349(MYLK4) 102269080(MYLK) 102275601(MYLK3) 102276600(MYLK2)
BIU: 109567347(MYLK2) 109572727(MYLK3) 109577322(MYLK4)
BBUB: 102395214(MYLK3) 102402899(MYLK) 102412646(MYLK4) 102415047(MYLK2)
CHX: 102181454(MYLK4) 102181980(MYLK3) 102182147(MYLK2) 102190641(MYLK)
OAS: 101111897(MYLK2) 101113784(MYLK3) 105603910 443334(MYLK)
ODA: 120868758(MYLK2) 120869929 120870611(MYLK) 120872918(MYLK3)
CCAD: 122421038(MYLK3) 122429898(MYLK4) 122444644(MYLK) 122448844(MYLK2)
SSC: 100157526(MYLK4) 100524288(MYLK2) 110260971(MYLK3) 396848(MYLK)
CFR: 102505869(MYLK2) 102511426(MYLK3) 102515628(MYLK4) 102518144(MYLK)
CBAI: 105065146(MYLK3) 105073583(MYLK2) 105079764(MYLK4) 105081637(MYLK)
CDK: 105086549(MYLK4) 105090282(MYLK) 105091178(MYLK3) 105100824(MYLK2)
BACU: 103005866(MYLK3) 103008421(MYLK) 103017148(MYLK2) 103020477(MYLK4)
LVE: 103077707(MYLK4) 103079981(MYLK2) 103083551(MYLK3) 103090456(MYLK)
OOR: 101275794(MYLK4) 101282343(MYLK) 101287286(MYLK3) 101289244(MYLK2)
DLE: 111165019(MYLK3) 111171109 111171669(MYLK) 111184432(MYLK2)
PCAD: 102982196(MYLK3) 102982293(MYLK2) 102982405(MYLK) 102984346(MYLK4)
PSIU: 116740421(MYLK2) 116744277(MYLK3) 116752864(MYLK) 116761512(MYLK4)
ECB: 100050092(MYLK4) 100053698(MYLK2) 100056559(MYLK3) 100070482(MYLK)
EPZ: 103547465(MYLK) 103548123(MYLK4) 103559162(MYLK2)
EAI: 106823711(MYLK) 106833720(MYLK2) 106842841(MYLK4) 106848229(MYLK3)
MYB: 102259949(MYLK2) 102262704(MYLK4)
MYD: 102755283(MYLK2) 102759237(MYLK) 102765242(MYLK4)
MMYO: 118663442(MYLK2) 118674227(MYLK3) 118675928(MYLK) 118677237(MYLK4)
MLF: 102416877(MYLK) 102427404(MYLK4) 102443301(MYLK2)
MNA: 107527759(MYLK2) 107535858(MYLK3) 107542256(MYLK4)
PKL: 118707748(MYLK2) 118719751(MYLK3) 118723823(MYLK4)
HAI: 109383050(MYLK3) 109384471(MYLK2) 109388085
DRO: 112296778(MYLK3) 112297606(MYLK4) 112303707(MYLK2) 112320512(MYLK)
SHON: 118985341(MYLK2) 118990733(MYLK) 119001158(MYLK3) 119003468(MYLK4)
AJM: 119036780(MYLK4) 119039481(MYLK) 119047724(MYLK2) 119049053(MYLK3)
PDIC: 114488355(MYLK3) 114490158(MYLK) 114496983(MYLK4) 114506860(MYLK2)
MMF: 118617212(MYLK) 118618547(MYLK2) 118626541(MYLK4) 118639281(MYLK3)
RFQ: 117013453(MYLK) 117016156(MYLK2) 117026833(MYLK4) 117034912(MYLK3)
PALE: 102878549(MYLK3) 102890778(MYLK) 102891799(MYLK2) 102894361(MYLK4)
PGIG: 120586716(MYLK) 120603520(MYLK2) 120607743(MYLK4) 120617226(MYLK3)
RAY: 107512864(MYLK2) 107516607(MYLK4) 107518034(MYLK)
MJV: 108385316 108393093(MYLK2) 108396719(MYLK) 108402114(MYLK3)
TOD: 119249184(MYLK3) 119251054(MYLK4) 119253977(MYLK2) 119257453(MYLK)
LAV: 100656405(MYLK2) 100665634(MYLK4) 100677183(MYLK)
MDO: 100015876(MYLK3) 100019648(MYLK) 100021377(MYLK4)
GAS: 123235311(MYLK3) 123235420(MYLK2) 123240591(MYLK4) 123242352(MYLK)
SHR: 100918090(MYLK4) 100925131(MYLK3)
PCW: 110196726(MYLK4) 110207445(MYLK3) 110208127(MYLK)
OAA: 100079091(MYLK4) 100091283(MYLK) 100092442(MYLK3)
GGA: 396356(MYLK2) 396445(MYLK) 415748(MYLK3) 420893(MYLK4) 428278(MYLKSML)
PCOC: 116227380(MYLK) 116227766(MYLK2) 116233855 116243997(MYLK3)
MGP: 100538449 100542497(MYLK2) 100543918(MYLK4) 100547989(MYLK) 100551067(MYLK3) 104914090
CJO: 107309576(MYLK4) 107316813(MYLK) 107319273(MYLK3) 107323244(MYLK2) 107325036
NMEL: 110388525 110393482(MYLK4) 110400604(MYLK) 110404229(MYLK3) 110408280(MYLK2)
APLA: 101793167(MYLK3) 101799935(MYLK4) 101800032 101803931(MYLK2)
ACYG: 106029897(MYLK) 106039596(MYLK3) 106046900 106048980(MYLK4)
TGU: 100218159(MYLK2) 100222880(MYLK4) 100226590(MYLK) 100232455(MYLK3) 115490676
LSR: 110468290(MYLK) 110469015(MYLK2) 110470711(MYLK4) 110475603 110475887(MYLK3)
SCAN: 103814310(MYLK) 103816608(MYLK3) 103818174(MYLK4) 103818839(MYLK2) 103821775
PMOA: 120497241(MYLK4) 120498294 120499105(MYLK) 120507140(MYLK2) 120508049(MYLK3)
OTC: 121331625(MYLK2) 121333829(MYLK3) 121337981(MYLK) 121340492(MYLK4)
PRUF: 121348636(MYLK2) 121348877(MYLK3) 121356511(MYLK) 121357753(MYLK4)
GFR: 102037734(MYLK4) 102037824(MYLK2) 102038683(MYLK3) 102044716(MYLK)
FAB: 101807258(MYLK) 101807804 101809550(MYLK3) 101813259(MYLK2) 101819869(MYLK4)
PHI: 102101059 102104582(MYLK2) 102106790(MYLK) 102111091(MYLK4) 102112254(MYLK3)
PMAJ: 107207555(MYLK) 107209810(MYLK3) 107213081(MYLK2) 107213960(MYLK4) 107215054
CCAE: 111923845(MYLK4) 111932252(MYLK) 111934649(MYLK3) 111938138(MYLK2) 111940063
CCW: 104687441(MYLK3) 104687839(MYLK2) 104694020(MYLK) 104698150(MYLK4) 104698538
ETL: 114058107(MYLK) 114060388(MYLK3) 114063040(MYLK2) 114070606 114071985
FPG: 101910574 101917440(MYLK3) 101922032(MYLK2) 101922066 101922502(MYLK)
FCH: 102050025(MYLK) 102051042(MYLK2) 102051053 102058744(MYLK3)
CLV: 102088141 102093425(MYLK2) 102094050(MYLK4) 102095697(MYLK3)
EGZ: 104122823(MYLK4) 104125557(MYLK3) 104128132(MYLK) 104132337
NNI: 104011279(MYLK3) 104018366(MYLK2) 104018798(MYLK) 104018914 104021845(MYLK4)
ACUN: 113476670(MYLK4) 113482370(MYLK) 113484301(MYLK3) 113489147
PADL: 103913791(MYLK3) 103919008(MYLK4) 103919603(MYLK) 103921929
AAM: 106482002(MYLK4) 106483357(MYLK3) 106495651 106497378(MYLK2) 106499178(MYLK)
AROW: 112961486(MYLK2) 112973819(MYLK)
NPD: 112949543(MYLK2) 112949847(MYLK3) 112960116(MYLK)
DNE: 112984366(MYLK3) 112987277(MYLK2) 112992025(MYLK4) 112994221(MYLK)
ASN: 102369318(MYLK2) 102371849(MYLK3) 102381743(MYLK) 102384108(MYLK4) 102387857
AMJ: 102557905 102561520(MYLK) 102568638(MYLK3) 102574082(MYLK4)
CPOO: 109310558(MYLK3) 109313073(MYLK2) 109314959(MYLK4) 109322804(MYLK)
GGN: 109289569 109292519(MYLK2) 109300981(MYLK4) 109301415(MYLK)
PSS: 102448460(MYLK4) 102458914(MYLK3) 102461616(MYLK)
CMY: 102929389(MYLK4) 102929671(MYLK) 102943339(MYLK3) 102943755
CPIC: 101934934(MYLK4) 101935997(MYLK3) 101945225 101950238(MYLK2)
TST: 117869384 117873146(MYLK4) 117885275(MYLK) 117886013(MYLK2) 117886754(MYLK3)
CABI: 116816818(MYLK2) 116823503(MYLK3) 116832025 116834662 116834732(MYLK)
MRV: 120374867(MYLK) 120380179(MYLK2) 120384598(MYLK3) 120397649(MYLK4)
ACS: 100557929(mylk) 100559335(mylk3) 100562117(mylk2) 100567803(mylk4)
PVT: 110076324(MYLK3) 110083010(MYLK) 110086708(MYLK2) 110090626(MYLK4)
SUND: 121914150(MYLK3) 121918581(MYLK) 121929527(MYLK2) 121929958(MYLK4)
PBI: 103059391(MYLK3) 103060340(MYLK) 112540892
PMUR: 107284832(MYLK4) 107290115(MYLK3)
TSR: 106543664(MYLK)
PGUT: 117657250(MYLK2) 117668851(MYLK) 117670916(MYLK3) 117672422(MYLK4)
VKO: 123020628(MYLK4) 123022348(MYLK3) 123027308(MYLK) 123036113(MYLK2)
PMUA: 114599828(MYLK2) 114600967(MYLK4) 114602252(MYLK3) 114603926(MYLK)
ZVI: 118086713(MYLK3) 118088799(MYLK2) 118090072 118091777(MYLK)
GJA: 107106205 107108885(MYLK3) 107113908(MYLK)
XLA: 108700969 108701256(mylk.L) 108701763(mylkl.L) 108702240(mylk.S) 108714133 108715487 108719107(mylk4.L) 108720081(mylk4.S)
XTR: 100489678(mylkl) 100497991(mylk4) 100498081(xkr7) 100498243(mylk3) 101730432(mylk)
NPR: 108785969 108787381(MYLK4) 108802964(MYLK3)
DRE: 100003770(mylk5) 449707(mylk4b) 492576(mylkb) 558685(mylk2) 561635(mylk3) 566845(mylk4a) 569510(mylka)
ELY: 117247106(mylk4b) 117251242(mylka) 117268957(mylk5) 117272282(mylk4a)
PLEP: 121951941(mylk4a) 121956704(mylk5) 121963547(mylk4b)
SLUC: 116038187(mylk4b) 116046022(mylk4a) 116059339(mylk5) 116064213(mylkb)
ECRA: 117950394(mylk4b) 117954407(mylk4a) 117957953(mylk5)
GAT: 120816425 120824084(mylk4b) 120827945(mylk5) 120833692(mylkb)
PPUG: 119209229(mylk4a) 119217141(mylk4b) 119220388(mylk5) 119228971(mylkb)
MSAM: 119892236(mylk5) 119900283(mylk4b) 119902037(mylk4a) 119903747
CUD: 121503777(mylk5) 121512603(mylk4b) 121519648(mylk4a) 121522726(mylka)
OAU: 116312478(mylk5) 116314400(mylk4b) 116318706(mylk4a) 116327386(mylka)
OML: 112138131(mylkb) 112146941(mylk5) 112147476(mylk4a) 112150925(mylk4b)
GAF: 122822561(mylk5) 122837983(mylk4b) 122839732(mylka) 122841247
CTUL: 119777375(mylkb) 119778873(mylk5) 119787700(mylk4b) 119795685(mylk4a)
KMR: 108239577(mylk4a) 108243578(mylk4b) 108243749(mylk5) 108251559
CSEM: 103392366(mylk) 103393163 103396270(mylk4)
SSEN: 122767287(mylk4a) 122780933(mylk4b) 122785605(mylk5) 122786945
HHIP: 117754929 117760149(mylk4b) 117766548(mylk5) 117778656(mylk4a)
XGL: 120788222(mylk5) 120791078(mylk4b) 120798572(mylk4a) 120801368
AANG: 118210822(mylk2) 118214168(mylka) 118214811 118220996(mylk5) 118225122(mylk4b) 118227419 118234506(mylk4a)
LOC: 102682466(mylk3) 102685356(mylk2) 102686027 102689935(mylk4) 102697050(mylk)
LCM: 102346967(MYLK3) 102352251
CMK: 103176059(mylk3) 103176418 103188646(mylk4a)
SPU: 581963
APLC: 110985015
SKO: 100313660
DME: Dmel_CG42347(sqa)
DGR: 6569001
CCAT: 101449028
BOD: 106614432
SCAC: 106085866
AAG: 5566696
AALB: 109425849
AME: 413190
ACER: 108001692
BIM: 100741566
BBIF: 117214693
BVK: 117233202
BVAN: 117166615
BTER: 100649174
BPYO: 122568737
MGEN: 117221146
NMEA: 116429762
CGIG: 122399422
PFUC: 122513304
VPS: 122637041
NVI: 100118913
TPRE: 106659804
CGLO: 123263504
FAS: 105263396
DAM: 107042590
AGIF: 122855096
CCIN: 107273710
TCA: 659444
LDC: 111507951
PXU: 106120759
PRAP: 110996706
ZCE: 119837503
TNL: 113500367
API: 100163829
RMD: 113552034
BTAB: 109031131
CLEC: 106664694
HHAL: 106683752
NLU: 111052189
FOC: 113206234
CSEC: 111862018
DMK: 116917201
PVM: 113830372
PJA: 122253882
RSAN: 119383249
VDE: 111251098
VJA: 111272050
TUT: 107363672
DPTE: 113799848
CSCU: 111630964
CEL: CELE_ZC373.4(mlck-1)
CBR: CBG_01967
GAE: 121387359
OBI: 106880214
LAK: 106163548
NVE: 5521343
ATEN: 116299105
 » show all
Reference
1  [PMID:6894756]
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  Title
Purification and characterization of smooth muscle myosin light chain kinase.
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J Biol Chem 256:7501-9 (1981)
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  Title
Human platelet myosin light chain kinase requires the calcium-binding protein calmodulin for activity.
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Proc Natl Acad Sci U S A 76:1653-7 (1979)
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  Title
Myosin light-chain kinase, a new enzyme from striated muscle.
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FEBS Lett 41:292-6 (1974)
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  Title
Characterization of chicken skeletal muscle myosin light chain kinase. Evidence for muscle-specific isozymes.
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J Biol Chem 260:1020-6 (1985)
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  Title
Characterization of the calmodulin-binding and catalytic domains in skeletal muscle myosin light chain kinase.
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J Biol Chem 260:11275-85 (1985)
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Mal TK, Skrynnikov NR, Yap KL, Kay LE, Ikura M.
  Title
Detecting protein kinase recognition modes of calmodulin by residual dipolar couplings in solution NMR.
  Journal
Biochemistry 41:12899-906 (2002)
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  Authors
Sobieszek A.
  Title
Enzyme kinetic characterization of the smooth muscle myosin phosphorylating system: activation by calcium and calmodulin and possible inhibitory mechanisms of antagonists.
  Journal
Biochim Biophys Acta 1450:77-91 (1999)
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Sobieszek A, Borkowski J, Babiychuk VS.
  Title
Purification and characterization of a smooth muscle myosin light chain kinase-phosphatase complex.
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J Biol Chem 272:7034-41 (1997)
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Fujita K, Ye LH, Sato M, Okagaki T, Nagamachi Y, Kohama K.
  Title
Myosin light chain kinase from skeletal muscle regulates an ATP-dependent interaction between actin and myosin by binding to actin.
  Journal
Mol Cell Biochem 190:85-90 (1999)
Other DBs
ExplorEnz - The Enzyme Database: 2.7.11.18
IUBMB Enzyme Nomenclature: 2.7.11.18
ExPASy - ENZYME nomenclature database: 2.7.11.18
BRENDA, the Enzyme Database: 2.7.11.18
CAS: 51845-53-5

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