KEGG   ENZYME: 2.7.11.18
Entry
EC 2.7.11.18                Enzyme                                 

Name
myosin-light-chain kinase;
[myosin-light-chain] kinase;
ATP:myosin-light-chain O-phosphotransferase;
calcium/calmodulin-dependent myosin light chain kinase;
MLCK;
MLCKase;
myosin kinase;
myosin light chain kinase;
myosin light chain protein kinase;
myosin light-chain kinase (phosphorylating);
smooth-muscle-myosin-light-chain kinase;
STK18
Class
Transferases;
Transferring phosphorus-containing groups;
Protein-serine/threonine kinases
Sysname
ATP:[myosin light chain] O-phosphotransferase
Reaction(IUBMB)
ATP + [myosin light chain] = ADP + [myosin light chain] phosphate [RN:R03150]
Reaction(KEGG)
R03150
Substrate
ATP [CPD:C00002];
myosin light chain [CPD:C01003]
Product
ADP [CPD:C00008];
myosin light chain phosphate [CPD:C03875]
Comment
Requires Ca2+ and calmodulin for activity. The 20-kDa light chain from smooth muscle myosin is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors, but more slowly.
History
EC 2.7.11.18 created 1986 as EC 2.7.1.117, transferred 2005 to EC 2.7.11.18
Orthology
K00907  myosin-light-chain kinase
Genes
HSA: 340156(MYLK4) 4638(MYLK) 85366(MYLK2) 91807(MYLK3)
PTR: 101057233(MYLK) 465143(MYLK3) 471840(MYLK4) 742591(MYLK2)
PPS: 100974667(MYLK3) 100978891(MYLK2) 100979422(MYLK) 100995666(MYLK4)
GGO: 101142619(MYLK3) 101143745(MYLK2) 101151517(MYLK) 101151667(MYLK4)
PON: 100171951(MYLK3) 100444714(MYLK4) 100452913(MYLK) 100453783(MYLK2)
NLE: 100585981(MYLK2) 100591151(MYLK) 100594745(MYLK4) 100604194 115831086(MYLK3)
MCC: 707361(MYLK4) 712830(MYLK2) 715422(MYLK) 716132(MYLK3)
MCF: 102122026(MYLK4) 102130711(MYLK) 102132754(MYLK3) 102145766(MYLK2)
CSAB: 103222295(MYLK4) 103228167(MYLK) 103232215(MYLK3) 103248051(MYLK2)
RRO: 104653717(MYLK2) 104655407(MYLK) 104655430(MYLK3) 104661238(MYLK4)
RBB: 108514260(MYLK4) 108517416(MYLK) 108524142(MYLK2) 108534775(MYLK3)
CJC: 100391801(MYLK2) 100392653(MYLK3) 100392656(MYLK4) 100409440(MYLK)
SBQ: 101029220(MYLK) 101038668(MYLK2) 101049056(MYLK4) 101050910(MYLK3)
MMU: 107589(Mylk) 213435(Mylk3) 228785(Mylk2) 238564(Mylk4)
MCAL: 110290127(Mylk2) 110300419(Mylk3) 110308670(Mylk4) 110311174(Mylk)
MPAH: 110319274(Mylk2) 110329346(Mylk) 110334129(Mylk4) 110337577(Mylk3)
RNO: 100911165(Mylk4) 103689985 117558(Mylk2) 288057(Mylk) 291926(Mylk3)
MUN: 110547228(Mylk) 110550095(Mylk2) 110552995(Mylk3) 110558635(Mylk4)
CGE: 100755963(Mylk2) 100756400(Mylk4) 100758406(Mylk) 100766446(Mylk3)
NGI: 103731078(Mylk2) 103737011(Mylk4) 103741992(Mylk)
HGL: 101701713(Mylk4) 101704236(Mylk) 101712444(Mylk2)
CCAN: 109677703 109691764(Mylk2) 109700226(Mylk4)
OCU: 100009051(MYLK2) 100353972(MYLK4)
TUP: 102476504(MYLK) 102489117(MYLK2) 102498032(MYLK4)
CFA: 475345(MYLK3) 477187(MYLK2) 488012(MYLK) 488190(MYLK4)
VVP: 112907401(MYLK4) 112909931(MYLK2) 112913506(MYLK) 112914947(MYLK3)
AML: 100469244(MYLK2) 100473453(MYLK4) 100474254(MYLK3) 100481263(MYLK)
UMR: 103665161(MYLK4) 103673818(MYLK3) 103674343(MYLK2) 103678824(MYLK)
UAH: 113252202(MYLK3) 113252350(MYLK) 113258196(MYLK2) 113264229(MYLK4)
ORO: 101368074(MYLK4) 101370711(MYLK3) 101378973(MYLK) 101381609(MYLK2)
FCA: 101088408(MYLK4) 101091744(MYLK3) 101095813(MYLK2)
PTG: 102951994(MYLK2) 102962607(MYLK3) 102968305(MYLK4) 102972928(MYLK)
PPAD: 109249481(MYLK4) 109266499(MYLK) 109270987(MYLK3) 109272590(MYLK2)
AJU: 106965987(MYLK3) 106976155(MYLK4) 106984088(MYLK) 106986013(MYLK2)
BTA: 338037(MYLK) 518025(MYLK4) 526379(MYLK3) 533378(MYLK2)
BOM: 102264349(MYLK4) 102269080(MYLK) 102275601(MYLK3) 102276600(MYLK2)
BIU: 109567347(MYLK2) 109572727(MYLK3) 109577322(MYLK4)
BBUB: 102395214(MYLK3) 102402899(MYLK) 102412646(MYLK4) 102415047(MYLK2)
CHX: 102181454(MYLK4) 102181980(MYLK3) 102182147(MYLK2) 102190641(MYLK)
OAS: 101111897(MYLK2) 101113784(MYLK3) 105603910 443334(MYLK)
SSC: 100157526(MYLK4) 100524288(MYLK2) 110260971(MYLK3) 396848(MYLK)
CFR: 102505869(MYLK2) 102511426(MYLK3) 102515628(MYLK4) 102518144(MYLK)
CDK: 105086549(MYLK4) 105090282(MYLK) 105091178(MYLK3) 105100824(MYLK2)
BACU: 103005866(MYLK3) 103008421(MYLK) 103017148(MYLK2) 103020477(MYLK4)
LVE: 103077707(MYLK4) 103079981(MYLK2) 103083551(MYLK3) 103090456(MYLK)
OOR: 101275794(MYLK4) 101282343(MYLK) 101287286(MYLK3) 101289244(MYLK2)
DLE: 111165019(MYLK3) 111171109 111171669(MYLK) 111184432(MYLK2)
PCAD: 102982196(MYLK3) 102982293(MYLK2) 102982405(MYLK) 102984346(MYLK4)
ECB: 100050092(MYLK4) 100053698(MYLK2) 100056559(MYLK3) 100070482(MYLK)
EPZ: 103547465(MYLK) 103548123(MYLK4) 103559162(MYLK2)
EAI: 106823711(MYLK) 106833720(MYLK2) 106842841(MYLK4) 106848229(MYLK3)
MYB: 102259949(MYLK2) 102262704(MYLK4)
MYD: 102755283(MYLK2) 102759237(MYLK) 102765242(MYLK4)
MNA: 107527759(MYLK2) 107542256(MYLK4)
HAI: 109383050(MYLK3) 109384471(MYLK2) 109388085
DRO: 112297606(MYLK4) 112303707(MYLK2) 112320512(MYLK)
PALE: 102890778(MYLK) 102891799(MYLK2) 102894361(MYLK4)
RAY: 107512864(MYLK2) 107516607(MYLK4) 107518034(MYLK)
MJV: 108385316 108393093(MYLK2) 108396719(MYLK) 108402114(MYLK3)
LAV: 100656405(MYLK2) 100665634(MYLK4) 100677183(MYLK)
MDO: 100015876(MYLK3) 100019648(MYLK) 100021377(MYLK4)
SHR: 100918090(MYLK4) 100925131(MYLK3)
PCW: 110196726(MYLK4) 110207445(MYLK3) 110208127(MYLK)
OAA: 100079091(MYLK4) 100091283(MYLK) 100092442(MYLK3) 114806177(MYLK2)
GGA: 396356(MYLK2) 396445(MYLK) 415748(MYLK3) 420893(MYLK4) 428278(MYLKSML)
MGP: 100538449 100542497(MYLK2) 100543918(MYLK4) 100547989(MYLK) 100551067(MYLK3) 104914090
CJO: 107309576(MYLK4) 107316813(MYLK) 107319273(MYLK3) 107323244(MYLK2) 107325036
NMEL: 110388525 110393482(MYLK4) 110400604(MYLK) 110404229(MYLK3) 110408280(MYLK2)
APLA: 101793167(MYLK3) 101799935(MYLK4) 101800032 101803931(MYLK2)
ACYG: 106029897(MYLK) 106039596(MYLK3) 106046900 106048980(MYLK4)
TGU: 100218159(MYLK2) 100222880(MYLK4) 100226590(MYLK) 100232455(MYLK3) 115490676
LSR: 110468290(MYLK) 110469015(MYLK2) 110470711(MYLK4) 110475603 110475887(MYLK3)
SCAN: 103814310(MYLK) 103816608(MYLK3) 103818174(MYLK4) 103818839(MYLK2) 103821775
GFR: 102037734(MYLK4) 102037824(MYLK2) 102038683(MYLK3) 102044716(MYLK)
FAB: 101807258(MYLK) 101807804 101809550(MYLK3) 101813259(MYLK2) 101819869(MYLK4)
PHI: 102101059 102104582(MYLK2) 102106790(MYLK) 102111091(MYLK4) 102112254(MYLK3)
PMAJ: 107207555(MYLK) 107209810(MYLK3) 107213081(MYLK2) 107213960(MYLK4) 107215054
CCAE: 111923845(MYLK4) 111932252(MYLK) 111934649(MYLK3) 111938138(MYLK2) 111940063
CCW: 104687441(MYLK3) 104687839(MYLK2) 104694020(MYLK) 104698150(MYLK4) 104698538
ETL: 114058107(MYLK) 114060388(MYLK3) 114063040(MYLK2) 114070606 114071985
FPG: 101910574 101917440(MYLK3) 101922032(MYLK2) 101922066 101922502(MYLK)
FCH: 102050025(MYLK) 102051042(MYLK2) 102051053 102058744(MYLK3)
CLV: 102088141 102093425(MYLK2) 102094050(MYLK4) 102095697(MYLK3)
EGZ: 104122823(MYLK4) 104125557(MYLK3) 104128132(MYLK) 104132337
NNI: 104011279(MYLK3) 104018366(MYLK2) 104018798(MYLK) 104018914 104021845(MYLK4)
ACUN: 113476670(MYLK4) 113482370(MYLK) 113484301(MYLK3) 113489147
PADL: 103913791(MYLK3) 103919008(MYLK4) 103919603(MYLK) 103921929
AAM: 106482002(MYLK4) 106483357(MYLK3) 106495651 106497378(MYLK2) 106499178(MYLK)
ASN: 102369318(MYLK2) 102371849(MYLK3) 102381743(MYLK) 102384108(MYLK4) 102387857
AMJ: 102557905 102561520(MYLK) 102568638(MYLK3) 102574082(MYLK4)
PSS: 102448460(MYLK4) 102458914(MYLK3) 102461616(MYLK)
CPIC: 101934934(MYLK4) 101935997(MYLK3) 101945225 101950238(MYLK2)
ACS: 100557929(mylk) 100559335(mylk3) 100562117(mylk2) 100567803(mylk4)
PVT: 110076324(MYLK3) 110083010(MYLK) 110086708(MYLK2) 110090626(MYLK4)
PBI: 103059391(MYLK3) 103060340(MYLK) 112540892
PMUR: 107284832(MYLK4) 107290115(MYLK3)
TSR: 106543664(MYLK)
PMUA: 114599828(MYLK2) 114600967(MYLK4) 114602252(MYLK3) 114603926(MYLK)
GJA: 107106205 107108885(MYLK3) 107113908(MYLK)
XTR: 100489678(mylkl) 100497991(mylk4) 100498081(xkr7) 100498243(mylk3) 101730432(mylk)
NPR: 108785969 108787381(MYLK4) 108802964(MYLK3)
DRE: 100003770(mylk5) 449707(mylk4b) 492576(mylkb) 558685(mylk2) 561635(mylk3) 566845(mylk4a) 569510(mylka)
CSEM: 103392366(mylk) 103393163 103396270(mylk4)
LCM: 102346967(MYLK3) 102352251
CMK: 103176059(mylk3) 103176418(mylk) 103188646
SPU: 581963
APLC: 110985015
SKO: 100313660
DME: Dmel_CG42347(sqa)
AAG: 5566696
AALB: 109425849
AME: 413190
BIM: 100741566
BTER: 100649174
NVI: 100118913
TCA: 659444
PRAP: 110996706
TNL: 113500367
API: 100163829
RMD: 113552034
BTAB: 109031131
CLEC: 106664694
PVM: 113830372
TUT: 107363672
DPTE: 113799848
CSCU: 111630964
PTEP: 107439970
CEL: CELE_ZC373.4(mlck-1)
CBR: CBG01967
BMY: Bm1_37415
OBI: 106880214
LAK: 106163548
NVE: 5521343
 » show all
Reference
1  [PMID:6894756]
  Authors
Adelstein RS, Klee CB.
  Title
Purification and characterization of smooth muscle myosin light chain kinase.
  Journal
J Biol Chem 256:7501-9 (1981)
Reference
2  [PMID:156362]
  Authors
Hathaway DR, Adelstein RS.
  Title
Human platelet myosin light chain kinase requires the calcium-binding protein calmodulin for activity.
  Journal
Proc Natl Acad Sci U S A 76:1653-7 (1979)
DOI:10.1073/pnas.76.4.1653
Reference
3  [PMID:4853304]
  Authors
Pires E, Perry SV, Thomas MA.
  Title
Myosin light-chain kinase, a new enzyme from striated muscle.
  Journal
FEBS Lett 41:292-6 (1974)
DOI:10.1016/0014-5793(74)81232-9
Reference
4  [PMID:3881420]
  Authors
Nunnally MH, Rybicki SB, Stull JT.
  Title
Characterization of chicken skeletal muscle myosin light chain kinase. Evidence for muscle-specific isozymes.
  Journal
J Biol Chem 260:1020-6 (1985)
Reference
5  [PMID:3897230]
  Authors
Edelman AM, Takio K, Blumenthal DK, Hansen RS, Walsh KA, Titani K, Krebs EG.
  Title
Characterization of the calmodulin-binding and catalytic domains in skeletal muscle myosin light chain kinase.
  Journal
J Biol Chem 260:11275-85 (1985)
Reference
6  [PMID:12390014]
  Authors
Mal TK, Skrynnikov NR, Yap KL, Kay LE, Ikura M.
  Title
Detecting protein kinase recognition modes of calmodulin by residual dipolar couplings in solution NMR.
  Journal
Biochemistry 41:12899-906 (2002)
DOI:10.1021/bi0264162
Reference
7  [PMID:10231558]
  Authors
Sobieszek A.
  Title
Enzyme kinetic characterization of the smooth muscle myosin phosphorylating system: activation by calcium and calmodulin and possible inhibitory mechanisms of antagonists.
  Journal
Biochim Biophys Acta 1450:77-91 (1999)
DOI:10.1016/S0167-4889(99)00038-5
Reference
8  [PMID:9054394]
  Authors
Sobieszek A, Borkowski J, Babiychuk VS.
  Title
Purification and characterization of a smooth muscle myosin light chain kinase-phosphatase complex.
  Journal
J Biol Chem 272:7034-41 (1997)
DOI:10.1074/jbc.272.11.7034
Reference
9  [PMID:10098974]
  Authors
Fujita K, Ye LH, Sato M, Okagaki T, Nagamachi Y, Kohama K.
  Title
Myosin light chain kinase from skeletal muscle regulates an ATP-dependent interaction between actin and myosin by binding to actin.
  Journal
Mol Cell Biochem 190:85-90 (1999)
Other DBs
ExplorEnz - The Enzyme Database: 2.7.11.18
IUBMB Enzyme Nomenclature: 2.7.11.18
ExPASy - ENZYME nomenclature database: 2.7.11.18
BRENDA, the Enzyme Database: 2.7.11.18
CAS: 51845-53-5

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