KEGG ENZYME: 2.7.11.19

ENZYME: 2.7.11.19

Entry

EC 2.7.11.19 Enzyme

Name

phosphorylase kinase;
dephosphophosphorylase kinase;
glycogen phosphorylase kinase;
PHK;
phosphorylase b kinase;
phosphorylase B kinase;
phosphorylase kinase (phosphorylating);
STK17

Class

Transferases;
Transferring phosphorus-containing groups;
Protein-serine/threonine kinases

Sysname

ATP:phosphorylase-b phosphotransferase

Reaction(IUBMB)

2 ATP + phosphorylase b = 2 ADP + phosphorylase a [RN:R00076]

Reaction(KEGG)

R00076

Substrate

ATP [CPD:C00002];
phosphorylase b [CPD:C02308]

Product

ADP [CPD:C00008];
phosphorylase a [CPD:C02307]

Comment

Requires Ca2+ and calmodulin for activity. The enzyme phosphorylates a specific serine residue in each of the subunits of the dimeric phosphorylase b. For muscle phosphorylase but not liver phosphorylase, this is accompanied by a further dimerization to form a tetrameric phosphorylase. The enzyme couples muscle contraction with energy production via glycogenolysis---glycolysis by catalysing the Ca2+-dependent phosphorylation and activation of glycogen phosphorylase b [5]. The gamma subunit of the tetrameric enzyme is the catalytic subunit.

History

EC 2.7.11.19 created 1961 as EC 2.7.1.38, transferred 2005 to EC 2.7.11.19

Orthology

K00871

phosphorylase kinase gamma subunit

Genes

HSA:	5260(PHKG1) 5261(PHKG2)

PTR:	741602(PHKG2) 746486(PHKG1)

PPS:	100983464(PHKG2) 100986631(PHKG1)

GGO:	101125302(PHKG2) 101134483(PHKG1)

PON:	100453698(PHKG1) 100455717(PHKG2)

NLE:	100588039(PHKG2) 100594015(PHKG1)

MCC:	100423096(PHKG2) 703091(PHKG1)

MCF:	102125953(PHKG1) 102128006(PHKG2)

CSAB:

103226033(PHKG1) 103231030 103231924(PHKG2)

RRO:	104661547(PHKG2) 104675874(PHKG1)

RBB:	108526509(PHKG1) 108530886(PHKG2)

CJC:	100412760(PHKG2) 100414478(PHKG1)

SBQ:	101045885(PHKG2) 101048522(PHKG1)

MMU:	18682(Phkg1) 68961(Phkg2)

RNO:	140671(Phkg2) 29353(Phkg1)

CGE:	100750910(Phkg2) 100754334(Phkg1)

NGI:	103726346(Phkg2) 103738953(Phkg1)

HGL:	101700591(Phkg2) 101707775(Phkg1)

CCAN:

109677759(Phkg1) 109682498(Phkg2)

OCU:	100009297(PHKG1) 100355661(PHKG2)

TUP:	102476302(PHKG1) 102480103(PHKG2) 102498323

CFA:	489784(PHKG1) 607275(PHKG2)

AML:	100477667(PHKG1) 100478501(PHKG2)

UMR:	103657874(PHKG2) 103670188(PHKG1)

ORO:	101366392(PHKG1) 101373618(PHKG2)

FCA:	101081184(PHKG1) 101092572(PHKG2)

PTG:	102958678(PHKG2) 102965580(PHKG1)

AJU:	106972855(PHKG1) 106979102(PHKG2)

BTA:	512670(PHKG2) 540682(PHKG1)

BOM:	102266225(PHKG1) 102279229(PHKG2)

BIU:	109578248(PHKG1) 109578294(PHKG2)

PHD:	102338518(PHKG2) 102339551(PHKG1)

CHX:	100861337(PHKG2) 102185139(PHKG1)

OAS:	100217403(PHKG2) 101109323(PHKG1)

SSC:	100310801(PHKG2) 397548(PHKG1)

CFR:	102509900(PHKG1) 102517940(PHKG2)

CDK:	105089705(PHKG1) 105102728(PHKG2)

BACU:

103011969(PHKG1) 103020540(PHKG2)

LVE:	103069260(PHKG1) 103082932(PHKG2)

OOR:	101270202(PHKG1) 101281573(PHKG2)

ECB:	100061404(PHKG1) 100065274(PHKG2)

EPZ:	103547228(PHKG2) 103566047(PHKG1)

EAI:	106831860(PHKG1) 106831907(PHKG2)

MYB:	102249201(PHKG2) 102254502(PHKG1)

MYD:	102751284(PHKG2) 102760309(PHKG1)

HAI:	109374179(PHKG2) 109376279(PHKG1)

RSS:	109437800(PHKG2) 109438915(PHKG1)

PALE:

102879938(PHKG1) 102882222(PHKG2)

LAV:	100660711(PHKG2) 100663820(PHKG1)

TMU:	101347179 101351533

MDO:	100012992(PHKG2) 100013442(PHKG1)

SHR:	100920220(PHKG1) 100927663(PHKG2)

OAA:	100088260(PHKG1) 100093441(PHKG2)

GGA:	417543(PHKG1)

MGP:	100546214(PHKG1)

CJO:	107322445(PHKG1)

APLA:

101793068(PHKG1)

ACYG:

106041427(PHKG1)

TGU:	100221300(PHKG1)

GFR:	102038175(PHKG1)

FAB:	101811164(PHKG1)

PHI:	102104524(PHKG1)

PMAJ:

107212749(PHKG1)

CCW:	104691086(PHKG1)

FPG:	101920077(PHKG1)

FCH:	102054686(PHKG1)

CLV:	102085380(PHKG1)

EGZ:	104131918(PHKG1)

ASN:	102372756(PHKG1) 102380957(PHKG2)

AMJ:	102572090(PHKG2) 102574725(PHKG1)

PSS:	102445349(PHKG2) 102455436(PHKG1)

CMY:	102934958(PHKG2) 102939493(PHKG1)

CPIC:

101932837(PHKG1) 101949847(PHKG2)

ACS:	100557980(phkg2)

PVT:	110084500(PHKG1) 110090073(PHKG2)

PBI:	103061914(PHKG2) 103065315(PHKG1)

GJA:	107111216(PHKG2) 107112491(PHKG1)

XLA:	108708334(phkg1.L) 373780(phkg1.S) 496328(phkg2.L)

XTR:	448255(phkg1) 779740(phkg2)

NPR:	108799187(PHKG1) 108803931(PHKG2)

DRE:	393937(phkg2) 554046(phkg1b) 565379(phkg1a)

SRX:	107715340 107730984 107743765 107747678 107754103 107756070

SANH:

107655811 107669401 107675026 107677094 107677866 107699969

SGH:	107563781 107566018 107567175 107567581 107574034

CCAR:

109069376 109069379 109097561 109097759

IPU:	108273676(phkg2) 108280550(phkg1)

AMEX:

103023937 103041098(phkg2) 103046290

TRU:	101077385(phkg2) 101080204(phkg1)

TNG:	GSTEN00001273G001 GSTEN00024080G001 GSTEN00035702G001

LCO:	104923220(phkg2) 104936522(phkg1)

NCC:	104946331(phkg2) 104953530 104964917

MZE:	101469795(phkg1) 101485765(phkg2)

OLA:	101165598(phkg2) 101167060(phkg1)

XMA:	102229601(phkg2) 102233585(phkg1)

PRET:

103474557(phkg1) 103481060(phkg2)

NFU:	107380364(phkg1) 107388054 107388073(phkg2)

CSEM:

103378132(phkg1) 103381724(phkg2)

LCF:	108886377(phkg1) 108886752(phkg2)

HCQ:	109525437(phkg1) 109530189(phkg2)

BPEC:

110154096 110159624(phkg2)

SASA:

100195216(phkg2) 106581056 106612828

ELS:	105021305 105030287(phkg1)

SFM:	108922452(phkg1)

LCM:	102345800(PHKG1) 102362563

CMK:	103188445(phkg1)

BFO:	BRAFLDRAFT_107074

CIN:

SPU:

APLC:

SKO:

DME:	Dmel_CG1830(PhKgamma)

DPO:	Dpse_GA14880

DAN:	Dana_GF20823

DER:	Dere_GG18438

DPE:	Dper_GL15144

DSE:	Dsec_GM13103

DSI:	Dsimw501_GD17041(Dsim_GD17041)

DWI:	Dwil_GK20076

DYA:	Dyak_GE15660

DGR:	Dgri_GH24413

DMO:	Dmoj_GI16230

DVI:	Dvir_GJ15921

MDE:

101900257

AGA:	AgaP_AGAP002647

AAG:

5578562

CQU:	CpipJ_CPIJ014338 CpipJ_CPIJ014339

AME:	107964213 550645(PhKgamma)

BIM:	100745483 105682157

BTER:

100644069 105666205

SOC:	105203940 105203941

AEC:

105144123

ACEP:

105624549

PBAR:

105434370

HST:	105183521 105183522

CFO:	105255563 105255564

LHU:

PGC:

NVI:

TCA:

DPA:

NVL:

BMOR:

DPL:	KGM_212724

PMAC:

PRAP:

PXY:

API:

DNX:	107163272 107170419

PHU:	Phum_PHUM607620

ZNE:

110830693

FCD:

110853985

DPX:	DAPPUDRAFT_303518

ISC:	IscW_ISCW015509

TUT:

107369709

CEL:	CELE_Y50D7A.3(Y50D7A.3)

CBR:

CBG15712

NAI:	NECAME_03497

BMY:

Bm1_44555

LOA:	LOAG_17265 LOAG_17266

TSP:

Tsp_00128

HRO:	HELRODRAFT_159557 HELRODRAFT_185431 HELRODRAFT_82909

LGI:	LOTGIDRAFT_209139

CRG:

105317472

MYI:

110462867

OBI:

106871486

LAK:	106157129 106172511

SMM:	Smp_098840

SHX:

MS3_01719

OVI:	T265_09098

NVE:	NEMVE_v1g94804

EPA:

ADF:

HMG:

AQU:

CSL:	COCSUDRAFT_67631

PIC:	PICST_32412(PAK3)

SPAA:

SPAPADRAFT_63791

PTM:	GSPATT00035369001 GSPATT00035512001 GSPATT00036542001

SMIN:

v1.2.023880.t1(symbB.v1.2.023880.t1)

PTI:	PHATRDRAFT_14702

SPAR:

SPRG_03052

» show all

Reference

1 [PMID:13315361]

Authors

KREBS EG, FISCHER EH.

Title

The phosphorylase b to a converting enzyme of rabbit skeletal muscle.

Journal

Biochim Biophys Acta 20:150-7 (1956)
DOI:10.1016/0006-3002(56)90273-6

Reference

2 [PMID:13538949]

Authors

KREBS EG, KENT AB, FISCHER EH.

Title

The muscle phosphorylase b kinase reaction.

Journal

J Biol Chem 231:73-83 (1958)

Reference

3 [PMID:13315351]

Authors

RALL TW, WOSILAIT WD, SUTHERLAND EW.

Title

The interconversion of phosphorylase a and phosphorylase b from dog heart muscle.

Journal

Biochim Biophys Acta 20:69-76 (1956)
DOI:10.1016/0006-3002(56)90264-5

Reference

4 [PMID:4029141]

Authors

Nikolaropoulos S, Sotiroudis TG.

Title

Phosphorylase kinase from chicken gizzard. Partial purification and characterization.

Journal

Eur J Biochem 151:467-73 (1985)
DOI:10.1111/j.1432-1033.1985.tb09125.x

Reference

5 [PMID:1931956]

Authors

Farrar YJ, Carlson GM.

Title

Kinetic characterization of the calmodulin-activated catalytic subunit of phosphorylase kinase.

Journal

Biochemistry 30:10274-9 (1991)

Reference

6 [PMID:7673209]

Authors

Dasgupta M, Blumenthal DK.

Title

Characterization of the regulatory domain of the gamma-subunit of phosphorylase kinase. The two noncontiguous calmodulin-binding subdomains are also autoinhibitory.

Journal

J Biol Chem 270:22283-9 (1995)
DOI:10.1074/jbc.270.38.22283

Sequence

[ocu:100009297]

Reference

7 [PMID:9362479]

Authors

Lowe ED, Noble ME, Skamnaki VT, Oikonomakos NG, Owen DJ, Johnson LN.

Title

The crystal structure of a phosphorylase kinase peptide substrate complex: kinase substrate recognition.

Journal

EMBO J 16:6646-58 (1997)
DOI:10.1093/emboj/16.22.6646