KEGG ENZYME: 2.7.11.19

ENZYME: 2.7.11.19

Entry

EC 2.7.11.19 Enzyme

Name

phosphorylase kinase;
dephosphophosphorylase kinase;
glycogen phosphorylase kinase;
PHK;
phosphorylase b kinase;
phosphorylase B kinase;
phosphorylase kinase (phosphorylating);
STK17

Class

Transferases;
Transferring phosphorus-containing groups;
Protein-serine/threonine kinases

Sysname

ATP:phosphorylase-b phosphotransferase

Reaction(IUBMB)

2 ATP + phosphorylase b = 2 ADP + phosphorylase a [RN:R00076]

Reaction(KEGG)

R00076

Substrate

ATP [CPD:C00002];
phosphorylase b [CPD:C02308]

Product

ADP [CPD:C00008];
phosphorylase a [CPD:C02307]

Comment

Requires Ca2+ and calmodulin for activity. The enzyme phosphorylates a specific serine residue in each of the subunits of the dimeric phosphorylase b. For muscle phosphorylase but not liver phosphorylase, this is accompanied by a further dimerization to form a tetrameric phosphorylase. The enzyme couples muscle contraction with energy production via glycogenolysis---glycolysis by catalysing the Ca2+-dependent phosphorylation and activation of glycogen phosphorylase b [5]. The gamma subunit of the tetrameric enzyme is the catalytic subunit.

History

EC 2.7.11.19 created 1961 as EC 2.7.1.38, transferred 2005 to EC 2.7.11.19

Orthology

K00871

phosphorylase kinase gamma subunit

Genes

HSA:	5260(PHKG1) 5261(PHKG2)

PTR:	741602(PHKG2) 746486(PHKG1)

PPS:	100983464(PHKG2) 100986631(PHKG1)

GGO:	101125302(PHKG2)

PON:	100453698(PHKG1) 100455717(PHKG2)

NLE:	100588039(PHKG2) 100594015 115830981(PHKG1)

MCC:	100423096(PHKG2) 703091(PHKG1)

MCF:	102125953(PHKG1) 102128006(PHKG2)

CSAB:

103226033(PHKG1) 103231924(PHKG2)

RRO:	104661547(PHKG2) 104675874(PHKG1)

RBB:	108526509(PHKG1) 108530886(PHKG2)

CJC:	100412760(PHKG2) 100414478(PHKG1)

SBQ:	101045885(PHKG2) 101048522(PHKG1)

MMU:	18682(Phkg1) 68961(Phkg2)

MCAL:

110294811(Phkg1) 110298655(Phkg2)

MPAH:

110312993(Phkg1) 110337850(Phkg2)

RNO:	140671(Phkg2) 29353(Phkg1)

MUN:	110547989(Phkg2) 110560975(Phkg1)

CGE:	100750910(Phkg2) 100754334(Phkg1)

NGI:	103726346(Phkg2) 103738953(Phkg1)

HGL:	101700591(Phkg2) 101707775(Phkg1)

CCAN:

109677759(Phkg1) 109682498(Phkg2)

OCU:	100009297(PHKG1) 100355661(PHKG2)

TUP:	102476302(PHKG1) 102480103(PHKG2) 102498323

CFA:	489784(PHKG1) 607275(PHKG2)

VVP:	112929247(PHKG2) 112934117(PHKG1)

AML:	100477667(PHKG1) 100478501(PHKG2)

UMR:	103657874(PHKG2) 103670188(PHKG1)

UAH:	113267697(PHKG2) 113267739(PHKG1)

ORO:	101366392(PHKG1) 101373618(PHKG2)

ELK:	111146405 111162228

FCA:	101081184(PHKG1) 101092572(PHKG2)

PTG:	102958678(PHKG2) 102965580(PHKG1)

PPAD:

109250661(PHKG1) 109258973(PHKG2)

AJU:	106972855(PHKG1) 106979102(PHKG2)

BTA:	512670(PHKG2) 540682(PHKG1)

BOM:	102266225(PHKG1) 102279229(PHKG2)

BIU:	109578248(PHKG1) 109578294(PHKG2)

BBUB:

102408486(PHKG2) 102414182(PHKG1)

CHX:	100861337(PHKG2) 102185139(PHKG1)

OAS:	100217403(PHKG2) 101109323(PHKG1)

SSC:	100310801(PHKG2) 397548(PHKG1)

CFR:	102509900(PHKG1) 102517940(PHKG2)

CDK:	105089705(PHKG1) 105102728(PHKG2)

BACU:

103011969(PHKG1) 103020540(PHKG2)

LVE:	103069260(PHKG1) 103082932(PHKG2)

OOR:	101270202(PHKG1) 101281573(PHKG2)

DLE:	111182088(PHKG1) 111182126(PHKG2)

PCAD:

102978084(PHKG2) 102982207(PHKG1)

ECB:	100061404(PHKG1) 100065274(PHKG2)

EPZ:	103547228(PHKG2) 103566047(PHKG1)

EAI:	106831860(PHKG1) 106831907(PHKG2)

MYB:	102249201(PHKG2) 102254502(PHKG1)

MYD:	102751284(PHKG2) 102760309(PHKG1)

MNA:	107524377(PHKG1) 107542003(PHKG2)

HAI:	109374179(PHKG2) 109376279(PHKG1)

DRO:	112304780(PHKG2) 112314866(PHKG1)

PALE:

102879938(PHKG1) 102882222(PHKG2)

RAY:	107503972(PHKG2) 107507449(PHKG1)

MJV:	108385379(PHKG2) 108405955(PHKG1)

LAV:	100660711(PHKG2) 100663820(PHKG1)

TMU:	101347179 101351533

MDO:	100012992(PHKG2) 100013442(PHKG1)

SHR:	100920220(PHKG1) 100927663(PHKG2)

PCW:	110221270(PHKG1) 110223219(PHKG2)

OAA:	100088260(PHKG1) 100093441(PHKG2)

GGA:	417543(PHKG1)

MGP:	100546214(PHKG1)

CJO:	107322445(PHKG1)

NMEL:

110407838(PHKG1)

APLA:

101793068(PHKG1)

ACYG:

106041427(PHKG1)

TGU:	100221300(PHKG1)

LSR:	110482022(PHKG1)

SCAN:

103820093(PHKG1)

GFR:	102038175(PHKG1)

FAB:	101811164(PHKG1)

PHI:	102104524(PHKG1)

PMAJ:

107212749(PHKG1)

CCAE:

111937725(PHKG1)

CCW:	104691086(PHKG1)

ETL:	114060505(PHKG1)

FPG:	101920077(PHKG1)

FCH:	102054686(PHKG1)

CLV:	102085380(PHKG1)

EGZ:	104131918(PHKG1)

NNI:	104016101(PHKG1)

ACUN:

113487119(PHKG1)

PADL:

103925605(PHKG1)

ASN:	102372756(PHKG1) 102380957(PHKG2)

AMJ:	102572090(PHKG2) 102574725(PHKG1)

PSS:	102445349(PHKG2) 102455436(PHKG1)

CMY:	102934958(PHKG2) 102939493(PHKG1)

CPIC:

101932837(PHKG1) 101949847(PHKG2)

ACS:	100557980(phkg2)

PVT:	110084500(PHKG1) 110090073(PHKG2)

PBI:	103061914(PHKG2) 103065315(PHKG1)

PMUR:

107287056(PHKG1) 107300286

TSR:	106552842(PHKG2)

PMUA:

114583711(PHKG2) 114585912(PHKG1)

GJA:	107111216(PHKG2) 107112491(PHKG1)

XLA:	108708334(phkg1.L) 373780(phkg1.S) 496328(phkg2.L)

XTR:	448255(phkg1) 779740(phkg2)

NPR:	108799187(PHKG1) 108803931(PHKG2)

DRE:	393937(phkg2) 554046(phkg1b) 565379(phkg1a)

SRX:	107715340 107730984 107743765 107747678 107754103 107756070

SANH:

107655811 107669401 107675026 107677094 107677866 107699969

SGH:	107563781 107566018 107567175 107567581 107574034

CCAR:

109069376 109069379 109097561 109097759

IPU:	108273676(phkg2) 108280550(phkg1)

PHYP:

113527058(phkg2) 113541799 113543328(phkg1)

AMEX:

103023937 103041098(phkg2) 103046290

EEE:	113573644(phkg1) 113583965(phkg2) 113584646

TRU:	101077385(phkg2) 101080204(phkg1)

TNG:	GSTEN00024080G001 GSTEN00035702G001

LCO:	104923220(phkg2) 104936522(phkg1)

NCC:	104946331(phkg2) 104964917

MZE:	101469795(phkg1) 101485765(phkg2)

ONL:	100694651(phkg1) 100694837(phkg2)

OLA:	101165598(phkg2) 101167060(phkg1)

XMA:	102229601(phkg2) 102233585(phkg1)

XCO:	114133398(phkg1) 114157788(phkg2)

PRET:

103474557(phkg1) 103481060(phkg2)

CVG:	107088313(phkg1) 107089513

NFU:	107380364(phkg1) 107388054 107388073(phkg2)

KMR:	108237211(phkg2) 108246020(phkg1a)

ALIM:

106515467(phkg1) 106517365

AOCE:

111564740(phkg1) 111584911(phkg2)

CSEM:

103378132(phkg1) 103381724(phkg2)

POV:	109627136(phkg2) 109629980(phkg1)

LCF:	108886377(phkg1) 108886752(phkg2)

SDU:	111219834(phkg2) 111228039(phkg1)

SLAL:

111650931(phkg1) 111658612(phkg2)

HCQ:	109525437(phkg1) 109530189(phkg2)

BPEC:

110154096 110159624(phkg2)

MALB:

109960264(phkg1) 109963558(phkg2)

SASA:

100195216(phkg2) 106581056 106612828

OTW:	112223679 112265471(phkg1)

SALP:

111956767 111977401 111982583

ELS:	105021305 105030287(phkg1)

SFM:	108922452(phkg1)

PKI:	111847182(phkg1)

LCM:	102345800(PHKG1) 102362563

CMK:	103188445(phkg1)

RTP:	109917579 109938585

BFO:

CIN:

SPU:

APLC:

SKO:

DME:	Dmel_CG1830(PhKgamma)

DER:

6551326

DSE:

6619897

DSI:	Dsimw501_GD17041(Dsim_GD17041)

DYA:	Dyak_GE15660

DAN:

6503515

DSR:

110184224

DPO:	Dpse_GA14880

DPE:

6600411

DMN:

108153016

DWI:

6641221

DGR:	Dgri_GH24413

DMO:	Dmoj_GI16230

DAZ:

DNV:

DHE:

DVI:

MDE:

LCQ:

AGA:	AgaP_AGAP002647

AAG:

5578562

AALB:

109418342 115253710

CQU:	CpipJ_CPIJ014338 CpipJ_CPIJ014339

AME:	107964213 550645

BIM:	100745483 105682157

BTER:

100644069

CCAL:

108628393

OBB:	114878897 114878899

SOC:

MPHA:

AEC:

PBAR:

VEM:

HST:

DQU:

CFO:

LHU:

PGC:

OBO:

PCF:

NVI:

CSOL:

MDL:

TCA:

DPA:

ATD:

NVL:

BMOR:

BMAN:

DPL:	KGM_212724

PMAC:

PRAP:

HAW:

TNL:

PXY:

API:

DNX:

AGS:

RMD:

BTAB:

CLEC:

PHU:	Phum_PHUM607620

ZNE:

110830693

FCD:

110853985

DPX:	DAPPUDRAFT_303518

PVM:

113815105

ISC:	IscW_ISCW015509

TUT:

DPTE:

CSCU:

PTEP:

CEL:	CELE_Y50D7A.3(Y50D7A.3)

CBR:

CBG15712

BMY:

Bm1_44555

LOA:	LOAG_17265 LOAG_17266

NAI:	NECAME_03497

TSP:

Tsp_00128

HRO:	HELRODRAFT_185431 HELRODRAFT_82909

LGI:	LOTGIDRAFT_209139

PCAN:

CRG:

MYI:

OBI:

LAK:	106157129 106172511

SMM:	Smp_098840

SHX:

MS3_01719

OVI:	T265_09098

EGL:

NVE:

EPA:

ADF:

AMIL:

PDAM:

SPIS:

DGT:	114523818 114524037

HMG:

100207615

AQU:

100641898

PTM:	GSPATT00035369001 GSPATT00035512001 GSPATT00036542001

PTI:	PHATRDRAFT_14702

PIF:	PITG_02565

PCU:	PC_RS05430

» show all

Reference

1 [PMID:13315361]

Authors

KREBS EG, FISCHER EH.

Title

The phosphorylase b to a converting enzyme of rabbit skeletal muscle.

Journal

Biochim Biophys Acta 20:150-7 (1956)
DOI:10.1016/0006-3002(56)90273-6

Reference

2 [PMID:13538949]

Authors

KREBS EG, KENT AB, FISCHER EH.

Title

The muscle phosphorylase b kinase reaction.

Journal

J Biol Chem 231:73-83 (1958)

Reference

3 [PMID:13315351]

Authors

RALL TW, WOSILAIT WD, SUTHERLAND EW.

Title

The interconversion of phosphorylase a and phosphorylase b from dog heart muscle.

Journal

Biochim Biophys Acta 20:69-76 (1956)
DOI:10.1016/0006-3002(56)90264-5

Reference

4 [PMID:4029141]

Authors

Nikolaropoulos S, Sotiroudis TG.

Title

Phosphorylase kinase from chicken gizzard. Partial purification and characterization.

Journal

Eur J Biochem 151:467-73 (1985)
DOI:10.1111/j.1432-1033.1985.tb09125.x

Reference

5 [PMID:1931956]

Authors

Farrar YJ, Carlson GM.

Title

Kinetic characterization of the calmodulin-activated catalytic subunit of phosphorylase kinase.

Journal

Biochemistry 30:10274-9 (1991)
DOI:10.1021/bi00106a027

Reference

6 [PMID:7673209]

Authors

Dasgupta M, Blumenthal DK.

Title

Characterization of the regulatory domain of the gamma-subunit of phosphorylase kinase. The two noncontiguous calmodulin-binding subdomains are also autoinhibitory.

Journal

J Biol Chem 270:22283-9 (1995)
DOI:10.1074/jbc.270.38.22283

Sequence

[ocu:100009297]

Reference

7 [PMID:9362479]

Authors

Lowe ED, Noble ME, Skamnaki VT, Oikonomakos NG, Owen DJ, Johnson LN

Title

The crystal structure of a phosphorylase kinase peptide substrate complex: kinase substrate recognition.

Journal

EMBO J 16:6646-58 (1997)
DOI:10.1093/emboj/16.22.6646