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Entry
EC 2.7.7.100                Enzyme                                 

Name
SAMP-activating enzyme;
UbaA (ambiguous);
SAMP-activating enzyme E1 (ambiguous)
Class
Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases
BRITE hierarchy
Sysname
ATP:[SAMP]-Gly-Gly adenylyltransferase
Reaction(IUBMB)
ATP + [SAMP]-Gly-Gly = diphosphate + [SAMP]-Gly-Gly-AMP [RN:R12099]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
[SAMP]-Gly-Gly [CPD:C21900]
Product
diphosphate [CPD:C00013];
[SAMP]-Gly-Gly-AMP [CPD:C21901]
Comment
Contains Zn2+. The enzyme catalyses the activation of SAMPs (Small Archaeal Modifier Proteins), which are ubiquitin-like proteins found only in the Archaea, by catalysing the ATP-dependent formation of a SAMP adenylate in which the C-terminal glycine of SAMP is bound to AMP via an acyl-phosphate linkage. The product of this activity can accept a sulfur atom to form a thiocarboxylate moiety that acts as a sulfur carrier involved in thiolation of tRNA and other metabolites such as molybdopterin. Alternatively, the enzyme can also catalyse the transfer of SAMP from its activated form to an internal cysteine residue, leading to a process termed SAMPylation (see EC 6.2.1.55, E1 SAMP-activating enzyme).
History
EC 2.7.7.100 created 2018
Orthology
K22837  SAMP-activating enzyme
Genes
CCRO: CMC5_032930
MRM: A7982_06715
AFG: AFULGI_00005400
APO: Arcpr_0731
AVE: Arcve_1268
AST: Asulf_01031
MBU: Mbur_0130(moeB)
MMET: MCMEM_0684
MMH: Mmah_0709
HAL: VNG_0084G(moeB)
HSL: OE_1140R(ubaA)
HHB: Hhub_1334(ubaA)
HALH: HTSR_0272
HHSR: HSR6_0258(moeB)
HMA: rrnAC2090(moeB)
HHI: HAH_2583(moeB)
NPH: NP_0052A(ubaA)
NMO: Nmlp_1065(ubaA)
HUT: Huta_0150
HTI: HTIA_0141
HMU: Hmuk_0279
HWA: HQ_1425A(ubaA)
HWC: Hqrw_1502(ubaA)
HVO: HVO_0558(ubaA)
HME: HFX_0529(thiF)
HAER: DU502_04115(moeB)
HALB: EKH57_09530(moeB)
HTU: Htur_3268
NMG: Nmag_1394(ubaA1)
NAT: NJ7G_2757
SALI: L593_07740
 » show all
Taxonomy
Reference
1  [PMID:21368171]
  Authors
Miranda HV, Nembhard N, Su D, Hepowit N, Krause DJ, Pritz JR, Phillips C, Soll D, Maupin-Furlow JA
  Title
E1- and ubiquitin-like proteins provide a direct link between protein conjugation and sulfur transfer in archaea.
  Journal
Proc Natl Acad Sci U S A 108:4417-22 (2011)
DOI:10.1073/pnas.1018151108
  Sequence
[hvo:HVO_0558]
Reference
2  [PMID:23140889]
  Authors
Maupin-Furlow JA
  Title
Ubiquitin-like proteins and their roles in archaea.
  Journal
Trends Microbiol 21:31-8 (2013)
DOI:10.1016/j.tim.2012.09.006
Reference
3  [PMID:27459543]
  Authors
Hepowit NL, de Vera IM, Cao S, Fu X, Wu Y, Uthandi S, Chavarria NE, Englert M, Su D, Sll D, Kojetin DJ, Maupin-Furlow JA
  Title
Mechanistic insight into protein modification and sulfur mobilization activities  of noncanonical E1 and associated ubiquitin-like proteins of Archaea.
  Journal
FEBS J 283:3567-3586 (2016)
DOI:10.1111/febs.13819
  Sequence
[hvo:HVO_0558]
Other DBs
ExplorEnz - The Enzyme Database: 2.7.7.100
IUBMB Enzyme Nomenclature: 2.7.7.100
ExPASy - ENZYME nomenclature database: 2.7.7.100
BRENDA, the Enzyme Database: 2.7.7.100

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