KEGG   ENZYME: 2.7.7.46
Entry
EC 2.7.7.46                 Enzyme                                 
Name
gentamicin 2''-nucleotidyltransferase;
gentamicin 2''-adenylyltransferase;
aminoglycoside adenylyltransferase;
gentamycin 2''-nucleotidyltransferase
Class
Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases
Sysname
NTP:gentamicin 2''-nucleotidyltransferase
Reaction(IUBMB)
nucleoside triphosphate + gentamicin = diphosphate + 2''-nucleotidylgentamicin [RN:R02432]
Reaction(KEGG)
R02432 > R09047
Substrate
nucleoside triphosphate [CPD:C00201];
gentamicin [CPD:C00505]
Product
diphosphate [CPD:C00013];
2''-nucleotidylgentamicin [CPD:C03565]
Comment
ATP, dATP, CTP, ITP and GTP can act as donors; kanamycin, tobramycin and sisomicin can also act as acceptors. The nucleotidyl residue is transferred to the 2-hydroxy of the 3-amino-3-deoxy-D-glucose moiety in the antibiotic.
History
EC 2.7.7.46 created 1976
Orthology
K17881  aminoglycoside 2''-adenylyltransferase
Genes
ECLEECNIH2_24600
ECLNECNIH4_23065
ECLIECNIH5_22890
KPNKPN_pKPN5p08204
KPZKPNIH27_26410
KOKKONIH1_31180
KOCAB185_01215
KQUAVR78_27585
KQVB8P98_29915
CFDCFNIH1_06100
CIRC2U53_00275(aadB)
KINAB182_00470
EBCC2U52_00390(aadB) C2U52_01405(aadB)
PMPPmu_03410(aadB)
PMEXH4W19_04355(aadB)
PPUHB479_27254
ABYABAYE3622(aadB)
ABALABLAC_p200010
NBLGJV52_06000(aadB)
 » show all
Reference
1  [PMID:6281224]
  Authors
Angelatou F, Litsas SB, Kontomichalou P.
  Title
Purification and properties of two gentamicin-modifying enzymes, coded by a single plasmid pPK237 originating from Pseudomonas aeruginosa.
  Journal
J Antibiot (Tokyo) 35:235-44 (1982)
DOI:10.7164/antibiotics.35.235
Reference
2  [PMID:4946513]
  Authors
Naganawa H, Yagisawa M, Kondo S, Takeuchi T, Umezawa H.
  Title
The structure determination of an enzymatic inactivation product of 3',4'-dideoxykanamycin B.
  Journal
J Antibiot (Tokyo) 24:913-4 (1971)
DOI:10.7164/antibiotics.24.913
Reference
3  [PMID:4946512]
  Authors
Yagisawa M, Naganawa H, Kondo S, Hamada M, Takeuchi T.
  Title
Adenylyldideoxykanamycin B, a product of the inactivation of dideoxykanamycin B by Escherichia coli carrying R factor.
  Journal
J Antibiot (Tokyo) 24:911-2 (1971)
DOI:10.7164/antibiotics.24.911
Other DBs
ExplorEnz - The Enzyme Database: 2.7.7.46
IUBMB Enzyme Nomenclature: 2.7.7.46
ExPASy - ENZYME nomenclature database: 2.7.7.46
BRENDA, the Enzyme Database: 2.7.7.46
CAS: 62213-33-6

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