KEGG ENZYME: 2.7.7.46

ENZYME: 2.7.7.46

Entry

EC 2.7.7.46                 Enzyme

Name

gentamicin 2''-nucleotidyltransferase;
gentamicin 2''-adenylyltransferase;
aminoglycoside adenylyltransferase;
gentamycin 2''-nucleotidyltransferase

Class

Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases

Sysname

NTP:gentamicin 2''-nucleotidyltransferase

Reaction(IUBMB)

nucleoside triphosphate + gentamicin = diphosphate + 2''-nucleotidylgentamicin [RN:R02432]

Reaction(KEGG)

R02432 > R09047

Substrate

nucleoside triphosphate [CPD:C00201];
gentamicin [CPD:C00505]

Product

diphosphate [CPD:C00013];
2''-nucleotidylgentamicin [CPD:C03565]

Comment

ATP, dATP, CTP, ITP and GTP can act as donors; kanamycin, tobramycin and sisomicin can also act as acceptors. The nucleotidyl residue is transferred to the 2-hydroxy of the 3-amino-3-deoxy-D-glucose moiety in the antibiotic.

History

EC 2.7.7.46 created 1976

Orthology

K17881

aminoglycoside 2''-adenylyltransferase

Genes

ECLE:

ECNIH2_24600

ECLN:

ECNIH4_23065

ECLI:

ECNIH5_22890

KPN:	KPN_pKPN5p08204

KPZ:	KPNIH27_26410

KOK:	KONIH1_31180

KOC:	AB185_01215

KQU:	AVR78_27585

KQV:	B8P98_29915

CFD:	CFNIH1_06100

CIR:	C2U53_00275(aadB)

KIN:	AB182_00470

EBC:	C2U52_00390(aadB) C2U52_01405(aadB)

PMP:	Pmu_03410(aadB)

PMEX:

H4W19_04355(aadB)

PPUH:

B479_27254

ABY:	ABAYE3622(aadB)

ABAL:

ABLAC_p200010

NBL:	GJV52_06000(aadB)

» show all

Reference

1 [PMID:6281224]

Authors

Angelatou F, Litsas SB, Kontomichalou P.

Title

Purification and properties of two gentamicin-modifying enzymes, coded by a single plasmid pPK237 originating from Pseudomonas aeruginosa.

Journal

J Antibiot (Tokyo) 35:235-44 (1982)
DOI:10.7164/antibiotics.35.235

Reference

2 [PMID:4946513]

Authors

Naganawa H, Yagisawa M, Kondo S, Takeuchi T, Umezawa H.

Title

The structure determination of an enzymatic inactivation product of 3',4'-dideoxykanamycin B.

Journal

J Antibiot (Tokyo) 24:913-4 (1971)
DOI:10.7164/antibiotics.24.913

Reference

3 [PMID:4946512]

Authors

Yagisawa M, Naganawa H, Kondo S, Hamada M, Takeuchi T.

Title

Adenylyldideoxykanamycin B, a product of the inactivation of dideoxykanamycin B by Escherichia coli carrying R factor.

Journal

J Antibiot (Tokyo) 24:911-2 (1971)
DOI:10.7164/antibiotics.24.911

Other DBs

ExplorEnz - The Enzyme Database:

2.7.7.46

IUBMB Enzyme Nomenclature:

2.7.7.46

ExPASy - ENZYME nomenclature database:

2.7.7.46

BRENDA, the Enzyme Database:

2.7.7.46

CAS:	62213-33-6

DBGET integrated database retrieval system