KEGG   ENZYME: 2.7.7.73Help
Entry
EC 2.7.7.73                 Enzyme                                 

Name
sulfur carrier protein ThiS adenylyltransferase;
thiF (gene name)
Class
Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases
BRITE hierarchy
Sysname
ATP:[ThiS] adenylyltransferase
Reaction(IUBMB)
ATP + [ThiS] = diphosphate + adenylyl-[ThiS] [RN:R07459]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
[ThiS]
Product
diphosphate [CPD:C00013];
adenylyl-[ThiS]
Comment
Binds Zn2+. The enzyme catalyses the adenylation of ThiS, a sulfur carrier protein involved in the biosynthesis of thiamine.
The enzyme shows significant structural similarity to ubiquitin-activating enzyme [3,4]. In Escherichia coli, but not in Bacillus subtilis, the enzyme forms a cross link from Cys-184 to the ThiS carboxy terminus (the position that is also thiolated) via an acyldisulfide [2].
History
EC 2.7.7.73 created 2011
Pathway
ec00730  Thiamine metabolism
ec01100  Metabolic pathways
Orthology
K03148  sulfur carrier protein ThiS adenylyltransferase
Genes
ECO: b3992(thiF)
ECJ: JW3956(thiF)
ECD: ECDH10B_4181(thiF)
EBW: BWG_3652(thiF)
ECOK: ECMDS42_3430(thiF)
ECE: Z5567(thiF)
ECS: ECs4915(thiF)
ECF: ECH74115_5460(thiF)
ETW: ECSP_5062(thiF)
ELX: CDCO157_4656(thiF)
EOJ: ECO26_5101(thiF)
EOI: ECO111_4809(thiF)
EOH: ECO103_4741(thiF)
ECOO: ECRM13514_5113(thiF)
ECOH: ECRM13516_4849(thiF)
ECG: E2348C_4299(thiF)
EOK: G2583_4810(thiF)
ESO: O3O_01450
ESM: O3M_23815
ESL: O3K_23895
ECW: EcE24377A_4534(thiF)
ELH: ETEC_4252
EUN: UMNK88_4833(thiF)
ECC: c4949(thiF)
ECP: ECP_4205
ECI: UTI89_C3827(thiF)
ECV: APECO1_2482(thiF)
ECX: EcHS_A4226(thiF)
ECM: EcSMS35_4440(thiF)
ECY: ECSE_4280
ECR: ECIAI1_4207(thiF)
ECQ: ECED1_4699(thiF)
ECK: EC55989_4477(thiF)
ECT: ECIAI39_4382(thiF)
EOC: CE10_4672(thiF)
EUM: ECUMN_4516(thiF)
ECZ: ECS88_4453(thiF)
ELO: EC042_4359(thiF)
ESE: ECSF_3846
EBR: ECB_03869(thiF)
EBD: ECBD_4040
EKF: KO11_02410(thiF)
EAB: ECABU_c45080(thiF)
EDJ: ECDH1ME8569_3852(thiF)
EIH: ECOK1_4468(thiF)
ELW: ECW_m4350(thiF)
ELL: WFL_21175(thiF)
ELC: i14_4539(thiF)
ELD: i02_4539(thiF)
ELP: P12B_c4104(thiF)
EBL: ECD_03869(thiF)
EBE: B21_03822(thiF)
ELF: LF82_2249(thiF)
ECOI: ECOPMV1_02797(thiF)
ECOJ: P423_22130
ECOS: EC958_5103(thiF)
EFE: EFER_3762(thiF)
EAL: EAKF1_ch1924(thiF)
EMA: C1192_18240(thiF)
STY: STY3723(thiF)
STT: t3469(thiF)
STM: STM4162(thiF)
SEO: STM14_5003(thiF)
SEY: SL1344_4101(thiF)
SEJ: STMUK_4146(thiF)
SEB: STM474_4348(thiF)
SEF: UMN798_4509(thiF)
SENR: STMDT2_40151(thiF)
SEND: DT104_41591(thiF)
SENI: CY43_21730
SPT: SPA3999(thiF)
SEK: SSPA3714
SEI: SPC_3993(thiF)
SEC: SCH_4043(thiF)
SHB: SU5_0241
SEE: SNSL254_A4494(thiF)
SENS: Q786_20385
SED: SeD_A4568(thiF)
SEG: SG3444(thiF)
SEL: SPUL_3422(thiF)
SEGA: SPUCDC_3408(thiF)
SET: SEN3948(thiF)
SENA: AU38_20430
SENO: AU37_20445
SENV: AU39_20465
SENQ: AU40_22840
SENL: IY59_20935
SEEP: I137_16355
SENB: BN855_42280(thiG)
SENE: IA1_20240
SBG: SBG_3636(thiF)
SBZ: A464_4174
SFL: SF4064(thiF)
SFX: S3671(thiF)
SFV: SFV_4064(thiF)
SFE: SFxv_4431(thiF)
SFN: SFy_5821
SFS: SFyv_5888
SFT: NCTC1_04410(thiF_2)
SSN: SSON_4165(thiF)
SDY: SDY_3734(thiF)
ENC: ECL_00253
ECLO: ENC_01310
EEC: EcWSU1_00219(thiF)
ECLX: LI66_01310
ECLY: LI62_01720
ECLZ: LI64_01445
ESA: ESA_03681
CSK: ES15_3621(thiF)
CTU: CTU_03030(thiF)
KPN: KPN_04374(thiF)
KPU: KP1_0232(thiF)
KPP: A79E_4813
KPT: VK055_3100(thiF)
KPE: KPK_5300(thiF)
KPR: KPR_0312(thiF)
KPJ: N559_4923
KPX: PMK1_01857(thiF_2)
KPNU: LI86_25390
KPNK: BN49_4466(thiF)
KVA: Kvar_4868
KOX: KOX_07955
KOE: A225_0250
EAE: EAE_08170
EAR: CCG32365
CKO: CKO_02994
CRO: ROD_37601(thiF)
CAMA: F384_21975
EBT: EBL_c37150(thiF)
LEW: DAI21_03275(thiF)
EBF: D782_4277
EBC: C2U52_06705(thiF)
YPE: YPO3741(thiF)
YPK: y0489(thiF)
YPA: YPA_3613
YPN: YPN_0225
YPM: YP_3104(thiF)
YPG: YpAngola_A0453(thiF)
YPZ: YPZ3_3301(thiF)
YPD: YPD4_3293(thiF)
YPX: YPD8_3293(thiF)
YPW: CH59_1664
YPJ: CH55_3286
YPV: BZ15_3985
YPL: CH46_1336
YPS: YPTB0288(thiF)
YPO: BZ17_2285
YPI: YpsIP31758_3855(thiF)
YPY: YPK_0345
YPB: YPTS_0309
YPQ: DJ40_2123
YPU: BZ21_3655
YPR: BZ20_1826
YPC: BZ23_3927
YPF: BZ19_3758
YEN: YE0291(thiF)
YEY: Y11_35001
YEW: CH47_3185
YET: CH48_2000
YEE: YE5303_43491(thiF)
YAL: AT01_2173
YFR: AW19_2904(thiF)
YIN: CH53_2100
YKR: CH54_2885
YRO: CH64_2889
YRU: BD65_1644
SPE: Spro_0282
SRL: SOD_c02430(thiF)
SPLY: Q5A_001345(thiF)
SMAF: D781_0260
SMW: SMWW4_v1c02880(thiF)
SMAR: SM39_4219(thiF)
SMAC: SMDB11_4386(thiF)
SERF: L085_05825
RAA: Q7S_20975
ECA: ECA0230(thiF)
PATR: EV46_01250
PATO: GZ59_02540(thiF)
PCT: PC1_0214
PEC: W5S_0229
SOD: Sant_3918(thiF)
DDD: Dda3937_00233(thiF)
DDQ: DDI_4556
EBI: EbC_02650(thiF)
TPTY: NCTC11468_00196(thiF)
PLU: plu0484(thiF)
PAY: PAU_00392(thiF)
PMR: PMI2779(thiF)
PMIB: BB2000_2790(thiF)
XBO: XBJ1_4030(thiF)
XBV: XBW1_0430(thiF)
XNE: XNC1_0584(thiF)
XNM: XNC2_0571(thiF)
XDO: XDD1_3617(thiF)
XPO: XPG1_3149(thiF)
PSI: S70_08570
PSX: DR96_1464
PRG: RB151_004040(thiF)
PHEI: NCTC12003_00402(thiF)
MMK: MU9_699
ETE: ETEE_2783(thiF)
LRI: NCTC12151_00409(thiF)
PSHI: SAMEA2665130_3035(thiF)
APL: APL_0533(thiF)
APJ: APJL_0523(thiF)
APA: APP7_0572(thiF)
VCH: VC0063
VCS: MS6_2554
VCE: Vch1786_I2343(thiF)
VCI: O3Y_00295
VCO: VC0395_A2450(thiF)
VCR: VC395_0117(thiF)
VCM: VCM66_0063(thiF)
VCL: VCLMA_A0059(thiF)
VCX: VAA049_55(thiF)
VCZ: VAB027_55(thiF)
VVU: VV1_0962
VVY: VV3205
VVL: VV93_v1c29240(thiF)
VPA: VP3025
VPB: VPBB_2856
VAG: N646_2120
VSP: VS_3096
VAN: VAA_00206
VAU: VANGNB10_cI2687c(thiF)
VSC: VSVS12_00161(thiF)
VTA: A3230(thiF)
VFI: VF_0034(thiF)
VSA: VSAL_I2985(thiF)
AWD: AWOD_I_0034(thiF)
PPR: PBPRA0108(BP0610)
SON: SO_2443(thiF)
SDN: Sden_1772
SFR: Sfri_2199
SAZ: Sama_1858
SBL: Sbal_2024
SLO: Shew_2094
SSE: Ssed_2015
SPL: Spea_2381
SHL: Shal_1902
SWD: Swoo_2590
SWP: swp_2332
SVO: SVI_2369
SPSW: Sps_01182
ILO: IL0767(thiF)
CPS: CPS_4642(thiF)
AMAL: I607_00610
AMAE: I876_00625
AMAO: I634_00615
AMAD: I636_00650
AMAI: I635_00650
AMAG: I533_00635
AMAC: MASE_00460
AAUS: EP12_00505
GPS: C427_5286
SALH: HMF8227_02268(thiF)
PIN: Ping_0362
FBL: Fbal_2032
AHA: AHA_0441(thiF)
ASA: ASA_3903(thiF)
AVR: B565_3623
AMED: B224_6005
ASR: WL1483_1377(thiF)
TAU: Tola_1076
BCI: BCI_0043(thiF)
BCIB: IM45_104
HMS: HMU13210(moeB)
CJE: Cj1046c(moeB)
CJB: BN148_1046c(moeB)
CJJ: CJJ81176_1067(thiF)
CJU: C8J_0983(moeB)
CJI: CJSA_0989(moeB)
CJM: CJM1_1021(thiF)
CJS: CJS3_1095(thiF)
CJEJ: N564_01013
CJEU: N565_01059
CJEN: N755_01052
CJEI: N135_01082
CJER: H730_06205
CJV: MTVDSCj20_1048(thiF)
CJY: QZ67_01119(moeZ)
CJQ: UC78_1003(moeZ)
CJR: CJE1190(thiF)
CJD: JJD26997_0735(thiF)
CFF: CFF8240_0074(thiF)
CFT: CFF04554_0074(thiF)
CFV: CFVI03293_0074(thiF)
CFX: CFV97608_0074(thiF)
CFZ: CSG_760
CAMP: CFT03427_0079(thiF)
CHA: CHAB381_1178(thiF)
CCO: CCC13826_1276(thiF)
CCOC: CCON33237_0911(thiF)
CLA: Cla_1149(thiF)
CLR: UPTC16701_1122(thiF)
CLM: UPTC16712_1153(thiF)
CLQ: UPTC4110_1132(thiF)
CLN: UPTC3659_1365(thiF)
CLL: CONCH_1103(thiF)
CCQ: N149_0981(thiF)
CCF: YSQ_03790
CCY: YSS_05655
CCOI: YSU_03820
CCOF: VC76_05090(moeZ)
CCOO: ATE51_01528(moeZ)
CAJ: CIG1485E_0484(thiF)
CIS: CINS_1110(thiF)
CVO: CVOL_1123(thiF)
CPEL: CPEL_1257(thiF)
CAMR: CAQ16704_1141(thiF)
CSM: CSUB8521_1315(thiF)
CSF: CSUB8523_1420(thiF)
CGRA: CGRAC_1781(thiF)
CHYO: CHH_1393(thiF)
CHV: CHELV3228_1280(thiF)
CSPF: CSF_0426(thiF)
CPIN: CPIN18020_0736(thiF)
CCUN: CCUN_1773(thiF)
CLX: CLAN_0984(thiF)
GLO: Glov_3072
PCA: Pcar_0611(thiF-2) Pcar_2513(thiF-1)
PPD: Ppro_1833
DES: DSOUD_2998(thiF)
DEU: DBW_1048
DVM: DvMF_0798
DDE: Dde_1557
DDS: Ddes_2168
DAS: Daes_1269
DPI: BN4_10828
PPRF: DPRO_1176
LIP: LI0506(thiF)
LIR: LAW_00521(thiF)
DBA: Dbac_0774
DRT: Dret_0729
DSF: UWK_01895
DTO: TOL2_C00580(thiF)
SAT: SYN_00438
AMA: AM088(thiF)
AMF: AMF_063(thiF)
APH: APH_1174(thiF)
APY: YYU_05420
APD: YYY_05490
APHA: WSQ_05475
ERU: Erum8480(probable_thiF)
ERW: ERWE_CDS_08990(thiF)
ERG: ERGA_CDS_08890(thiF)
ECN: Ecaj_0885
ECH: ECH_1107(thiF)
ECHA: ECHHL_0074
ECHP: ECHWP_0073
EHH: EHF_0078
BSU: BSU11700(thiF)
BSR: I33_1304
BSL: A7A1_0987
BSH: BSU6051_11700(thiF)
BSUT: BSUB_01272(thiF)
BSUL: BSUA_01272(thiF)
BSUS: Q433_06610
BSS: BSUW23_05915(thiF)
BST: GYO_1475
BSO: BSNT_07613(thiF)
BSQ: B657_11700(thiF)
BSX: C663_1198(thiF)
BLI: BL01587(thiF)
BLD: BLi01264(thiF)
BLH: BaLi_c13990(thiF)
BAY: RBAM_011710(thiF)
BAQ: BACAU_1132(thiF)
BYA: BANAU_1077(thiF)
BAMP: B938_05695
BAML: BAM5036_1071(thiF)
BAMA: RBAU_1133(thiF)
BAMN: BASU_1110(thiF)
BAMB: BAPNAU_2624(thiF)
BAMT: AJ82_06580
BAMY: V529_11190(thiF)
BMP: NG74_01185(thiF_1)
BAO: BAMF_1244(thiF1)
BAZ: BAMTA208_11750(thiF)
BQL: LL3_01251(thiF)
BXH: BAXH7_02399(thiF)
BQY: MUS_1215(thiF)
BAMI: KSO_013985
BAMC: U471_11680
BAMF: U722_05970
BATR: TD68_03075
BPU: BPUM_1099
BPUM: BW16_06235
BPUS: UP12_05835
BJS: MY9_1275
BACW: QR42_05670
BACP: SB24_04015
BACB: OY17_08555
BACY: QF06_04800
BACL: BS34A_12930(thiF_1)
BALM: BsLM_1224
BGY: BGLY_1283(thiF)
LBH: Lbuc_2168
LBN: LBUCD034_2274(thiF)
LME: LEUM_1157
LMM: MI1_05105
LMK: LMES_0966
LKI: LKI_04015
WPA: CO680_08290(thiF)
CAC: CA_C2923
CAE: SMB_G2959(ygdL)
CAY: CEA_G2930
CPE: CPE1602
CPF: CPF_1855(thiF)
CPR: CPR_1572(thiF)
CTC: CTC_01750(moeB)
CTET: BN906_01897(moeB)
CNO: NT01CX_1503(thiF)
CBN: CbC4_0676(thiF)
CBE: Cbei_0647
CBZ: Cbs_0647
CBEI: LF65_00720
CKL: CKL_1638(thiF)
CKR: CKR_1523
CSR: Cspa_c49020(thiF1)
CSB: CLSA_c25150(thiF)
CLD: CLSPO_c21790(thiF)
CACE: CACET_c37750(thiF2)
AMT: Amet_4236
CTH: Cthe_0600
CCEL: CCDG5_1408
HSC: HVS_07280(thiF)
RTO: RTO_25280
BHAN: CGC63_13275(thiF)
CPRO: CPRO_15970(moeB_1)
CDF: CD630_17030(thiF)
PDC: CDIF630_01891(thiF)
CDC: CD196_1627(thiF)
CDL: CDR20291_1602(thiF)
PDF: CD630DERM_17030(thiF)
DRM: Dred_0075
DAE: Dtox_1682
PTH: PTH_0642
ELM: ELI_0851
TTE: TTE2471(ThiF)
THX: Thet_1753
TIT: Thit_0463
TKI: TKV_c08380(thiF)
CHY: CHY_1264(moeB)
TPZ: Tph_c17850(thiF)
CSC: Csac_0253
ATE: Athe_0371
FMA: FMG_0065
APR: Apre_1633
CAD: Curi_c11650(thiF1)
VPR: Vpar_0458
MED: MELS_2199
MHG: MHY_09380
PUF: UFO1_1305
AIN: Acin_2102(thiF)
BAD: BAD_0090
BADL: BADO_0099
BDE: BDP_0148(thiF)
BDN: BBDE_0138
BTP: D805_0147
BKS: BBKW_0104
BANG: BBAG_1425
BCAT: BBCT_0077
BPSC: BBPC_0090
BSCA: BBSC_0176
GVA: HMPREF0424_0777(thiF)
ELE: Elen_1748
PBU: L21SP3_01124(moeZ)
PBAS: SMSP2_01990(thiF)
PBP: STSP1_01179(moeB_1)
FNU: FN1755
FPD: CTM68_07160(thiF)
FVA: FV113G1_17900(thiF)
FSC: FSU_0171
BLQ: L21SP5_00494(moeB)
CACI: CLOAM1487
KOL: Kole_0998
DDF: DEFDS_1999(thiF)
DTH: DICTH_1551(thiF)
DTU: Dtur_1659
MARC: AR505_0103
 » show all
Taxonomy
Reference
1  [PMID:9632726]
  Authors
Taylor SV, Kelleher NL, Kinsland C, Chiu HJ, Costello CA, Backstrom AD, McLafferty FW, Begley TP
  Title
Thiamin biosynthesis in Escherichia coli. Identification of ThiS thiocarboxylate  as the immediate sulfur donor in the thiazole formation.
  Journal
J Biol Chem 273:16555-60 (1998)
DOI:10.1074/jbc.273.26.16555
  Sequence
[eco:b3992]
Reference
2  [PMID:11438688]
  Authors
Xi J, Ge Y, Kinsland C, McLafferty FW, Begley TP
  Title
Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: identification of an acyldisulfide-linked protein--protein conjugate that is functionally analogous to the ubiquitin/E1 complex.
  Journal
Proc Natl Acad Sci U S A 98:8513-8 (2001)
DOI:10.1073/pnas.141226698
  Sequence
[eco:b3992]
Reference
3  [PMID:15896804]
  Authors
Duda DM, Walden H, Sfondouris J, Schulman BA.
  Title
Structural analysis of Escherichia coli ThiF.
  Journal
J Mol Biol 349:774-86 (2005)
DOI:10.1016/j.jmb.2005.04.011
  Sequence
[eco:b3992]
Reference
4  [PMID:16388576]
  Authors
Lehmann C, Begley TP, Ealick SE.
  Title
Structure of the Escherichia coli ThiS-ThiF complex, a key component of the sulfur transfer system in thiamin biosynthesis.
  Journal
Biochemistry 45:11-9 (2006)
DOI:10.1021/bi051502y
  Sequence
[eco:b3992]
Other DBs
ExplorEnz - The Enzyme Database: 2.7.7.73
IUBMB Enzyme Nomenclature: 2.7.7.73
ExPASy - ENZYME nomenclature database: 2.7.7.73
BRENDA, the Enzyme Database: 2.7.7.73

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