KEGG   ENZYME: 2.7.7.97Help
Entry
EC 2.7.7.97                 Enzyme                                 

Name
3-hydroxy-4-methylanthranilate adenylyltransferase;
acmA (gene name);
sibE (gene name);
actinomycin synthase I;
4-MHA-activating enzyme;
ACMS I;
actinomycin synthetase I;
4-MHA pentapeptide lactone synthase AcmA
Class
Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases
BRITE hierarchy
Sysname
ATP:3-hydroxy-4-methylanthranilate adenylyltransferase
Reaction(IUBMB)
ATP + 3-hydroxy-4-methylanthranilate = diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate [RN:R11554]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
3-hydroxy-4-methylanthranilate [CPD:C03986]
Product
diphosphate [CPD:C00013];
3-hydroxy-4-methylanthranilyl-adenylate
Comment
The enzyme, characterized from the bacteria Streptomyces anulatus and Streptosporangium sibiricum, activates 3-hydroxy-4-methylanthranilate, a precursor of actinomycin antibiotics and the antitumor antibiotic sibiromycin, to an adenylate form, so it can be loaded onto a dedicated aryl-carrier protein.
History
EC 2.7.7.97 created 2016
Orthology
K21284  3-hydroxy-4-methylanthranilate adenylyltransferase
Genes
NBR: O3I_021160
SCB: SCAB_69851
SBH: SBI_01606
SRC: M271_02960
SLD: T261_0691
KSK: KSE_65160
RTX: TI83_07420
RTC: APU90_09660
KFL: Kfla_6074
SESP: BN6_21380
KAL: KALB_2251
ACTI: UA75_06460
ACAD: UA74_06460
ALL: CRK56726
SAQ: Sare_2681
MIL: ML5_4902
ACTN: L083_2872
CAI: Caci_3250
 » show all
Taxonomy
Reference
1  [PMID:10212227]
  Authors
Pfennig F, Schauwecker F, Keller U
  Title
Molecular characterization of the genes of actinomycin synthetase I and of a 4-methyl-3-hydroxyanthranilic acid carrier protein involved in the assembly of the acylpeptide chain of actinomycin in Streptomyces.
  Journal
J Biol Chem 274:12508-16 (1999)
DOI:10.1074/jbc.274.18.12508
  Sequence
Reference
2  [PMID:21612226]
  Authors
Giessen TW, Kraas FI, Marahiel MA
  Title
A four-enzyme pathway for 3,5-dihydroxy-4-methylanthranilic acid formation and incorporation into the antitumor antibiotic sibiromycin.
  Journal
Biochemistry 50:5680-92 (2011)
DOI:10.1021/bi2006114
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.7.7.97
IUBMB Enzyme Nomenclature: 2.7.7.97
ExPASy - ENZYME nomenclature database: 2.7.7.97
BRENDA, the Enzyme Database: 2.7.7.97

DBGET integrated database retrieval system