KEGG   ENZYME: 2.7.8.12
Entry
EC 2.7.8.12                 Enzyme                                 

Name
teichoic acid poly(glycerol phosphate) polymerase;
teichoic-acid synthase;
cytidine diphosphoglycerol glycerophosphotransferase;
poly(glycerol phosphate) polymerase;
teichoic acid glycerol transferase;
glycerophosphate synthetase;
CGPTase;
CDP-glycerol glycerophosphotransferase (ambiguous);
Tag polymerase;
CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase;
tagF (gene name);
tarF (gene name) (ambiguous)
Class
Transferases;
Transferring phosphorus-containing groups;
Transferases for other substituted phosphate groups
Sysname
CDP-glycerol:4-O-[(2R)-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol glycerophosphotransferase
Reaction(IUBMB)
n CDP-glycerol + 4-O-[(2R)-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = n CMP + 4-O-{poly[(2R)-glycerophospho]-(2R)-glycerophospho}-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Substrate
CDP-glycerol [CPD:C00513];
4-O-[(2R)-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Product
CMP [CPD:C00055];
4-O-{poly[(2R)-glycerophospho]-(2R)-glycerophospho}-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Comment
Involved in the biosynthesis of poly glycerol phosphate teichoic acids in bacterial cell walls. This enzyme adds 30--50 glycerol units to the linker molecule, but only after it has been primed with the first glycerol unit by EC 2.7.8.44, teichoic acid poly(glycerol phosphate) primase. cf. EC 2.7.8.45, teichoic acid glycerol-phosphate transferase.
History
EC 2.7.8.12 created 1972, modified 1982, modified 2017
Orthology
K09809  CDP-glycerol glycerophosphotransferase
Genes
ECC: c3694
ESE: ECSF_2780
CSI: P262_04923
CMJ: AFK66_016625
CBRA: A6J81_12080
CIF: AL515_05955
HDE: HDEF_0197
KAS: KATP_16030
IZH: FEM41_21455
ERWI: GN242_00025
ANS: ArsFIN_07500(tagB)
APJ: APJL_1611(cps3D)
APA: APP7_1639
ASU: Asuc_0817
BTO: WQG_4240
BTRE: F542_17720
BTRH: F543_19570
PPR: PBPRA2712(TAGF)
PALL: UYA_15020
SBL: Sbal_2320
SPL: Spea_1421
SWD: Swoo_1691
SVO: SVI_1504
SPSW: Sps_00158
SKH: STH12_01999(tagF)
KKO: Kkor_2549
NEU: NE1370
AZO: azo2701
AOA: dqs_2836
CJI: CJSA_1365(tagF)
CJER: H730_08190
CJQ: UC78_1373(tagF)
CLA: CLA_0297
CLX: CLAN_0120
COJ: CORN_0306
DPI: BN4_12068
DAT: HRM2_18180(tagB2)
PSF: PSE_0948
BSU: BSU35720(tagF)
BSR: I33_3701
BSL: A7A1_1147
BSH: BSU6051_35720(tagF)
BSUT: BSUB_03805(tagF)
BSUL: BSUA_03805(tagF)
BSUS: Q433_19575
BSQ: B657_35720(tagF)
BSX: C663_3464(tarF)
BSS: BSUW23_17545(tarF)
BST: GYO_3927 GYO_3928(tarF)
BLI: BL02462 BL02463(tagF)
BLD: BLi03816 BLi03817(tagF)
BAQ: BACAU_3315(tagF)
BYA: BANAU_3474(tagF)
BAMP: B938_16870
BAML: BAM5036_3208(tagF)
BAMA: RBAU_3424(tagF)
BAMN: BASU_3202(tagF)
BAMB: BAPNAU_3482(tagF)
BAMT: AJ82_18550
BAMY: V529_35530(tagF)
BMP: NG74_03461(tagF)
BAO: BAMF_3411(tagF)
BAZ: BAMTA208_18075(tagF)
BQL: LL3_03706(tagF)
BXH: BAXH7_03695(tagF)
BQY: MUS_3913(tagF)
BAMI: KSO_002645
BAMC: U471_34120
BAMF: U722_17615
BSON: S101395_00589(tagF)
BMOJ: HC660_34630(tagF)
BPUM: BW16_16290
BJS: MY9_3625
BACP: SB24_12270
BACB: OY17_00105
BACY: QF06_16270
BACL: BS34A_38850(tagF)
BALM: BsLM_3588
BFD: NCTC4823_03174(tagF_1) NCTC4823_03749(tagF_2) NCTC4823_03762(tagF_3)
BCOA: BF29_3252(tagF)
GTN: GTNG_3260
GEA: GARCT_03211(tagF_1) GARCT_03217(tagF_2)
VPN: A21D_00861(tagF)
PASA: BAOM_4713(tagF) BAOM_4714
BLEN: NCTC4824_03822(tagF_1) NCTC4824_03831(tagF_2) NCTC4824_03832(tagF_3)
SER: SERP1960(tagF)
SEPP: SEB_01955
SEPS: DP17_896(tagF)
SHA: SH0239 SH2250(tagF)
SHH: ShL2_02098(tarF_3)
SSP: SSP0204
SDT: SPSE_0280(tagF1) SPSE_0281(tagF2) SPSE_0284
SHU: SHYC_01830(tagF-1) SHYC_01835(tagF-2) SHYC_10220
SCAP: AYP1020_1552(tagF_2)
SSIM: SAMEA4384339_0182(tagF2_1) SAMEA4384339_0183(tagF2_2) SAMEA4384339_0214(tagF_3)
MCAK: MCCS_08860
LMOG: BN389_11160(tagF)
LMP: MUO_05685
LMOL: LMOL312_1083(tagF)
LMOX: AX24_02835
LMQ: LMM7_1113
LML: lmo4a_1091(tagF)
LMON: LMOSLCC2376_1054(tagF)
LMOO: LMOSLCC2378_1100(tagF)
LMOA: LMOATCC19117_1105(tagF)
LMOK: CQ02_05645
LIN: lin1074
LWE: lwe1064
LIV: LIV_1030
LGZ: NCTC10812_00475(tagF_1) NCTC10812_01708(tagF_4)
PSAB: PSAB_18505
JEO: JMA_33880
KZO: NCTC404_00412(tagF_1) NCTC404_02316(tagF_3)
LLA: L146188(tagX) L151480(tagF)
LLK: LLKF_0955(tagF)
LLT: CVCAS_0891(tagF)
LLS: lilo_0875(tagF)
LLJ: LG36_1398(tagF)
LLM: llmg_1604(tagF)
LLW: kw2_0920(tagF)
SNE: SPN23F03290(wchX)
SNP: SPAP_0386(wcwU)
SGA: GALLO_0954(cpsL)
SGG: SGGBAA2069_c09290(cpsL)
LJF: FI9785_1516(tagB)
LJH: LJP_1477c
LHE: lhv_0546
LHV: lhe_0540
LHH: LBH_0438
LHD: HUO_09400
LPL: lp_0268(tagF1)
LPT: zj316_0467(tagF1) zj316_1211(tagB) zj316_2p10
LPS: LPST_C0952(tagB)
LPZ: Lp16_0237
LFE: LAF_0091
LFR: LC40_0071
LBN: LBUCD034_1143(tagf1) LBUCD034_1700(tagf3)
PCE: PECL_1254
LCI: LCK_01329(tagZ)
LKI: LKI_07870
LGS: LEGAS_0531(tagF)
EFA: EF2493
MPS: MPTP_0823
MPX: MPD5_1115
CRN: CAR_c17200(tagF)
CARC: NY10_1106
CPE: CPE0482
ESR: ES1_01610
ESU: EUS_19710
RUM: CK1_38940
BHU: bhn_I2291
CCT: CC1_07490
CSO: CLS_25670
RTO: RTO_16670
ERH: ERH_0432
SCO: SCO2590(SCC123.28) SCO2997(SCE99.04)
SMA: SAVERM_5079(tagF)
SMAL: SMALA_3090
SCT: SCAT_4238(tagF)
SBH: SBI_06508
SVE: SVEN_2375
STRM: M444_12660
SRW: TUE45_03582(tagF_4)
SLE: sle_16850(sle_16850) sle_42870(sle_42870) sle_46440(sle_46440)
SLAU: SLA_2269
SALJ: SMD11_4229
SLX: SLAV_24610(tagF2) SLAV_33120(tagF3)
SNF: JYK04_01066(tagF) JYK04_01227(tarF_1) JYK04_03224(tarF_2)
SCYG: S1361_16125(tagF4)
LXL: KDY119_02605(tagF)
LXX: Lxx17460(tagF)
MTS: MTES_3147
MOO: BWL13_01667(tagF) BWL13_01669(tarF_1)
MOY: CVS54_02422(tagF)
AAGI: NCTC2676_1_01715(tagF_1) NCTC2676_1_01716(tagF_2)
BCV: Bcav_1199
JDE: Jden_1847
IDO: I598_2226(tagF_1) I598_3353(tagF_3)
SERJ: SGUI_1316
BLIN: BLSMQ_3088
MPH: MLP_25810
TFU: Tfu_2178
NAL: B005_3643
STRR: EKD16_07695(tagF)
NCX: Nocox_04755(epsJ2) Nocox_36310(tagF2)
GOB: Gobs_1280
SAQ: Sare_0522
MIL: ML5_0719
SNA: Snas_0745
AVC: NCTC10951_00255(tagF_1) NCTC10951_00257(tagF_2)
LBA: Lebu_2022
FSC: FSU_1413
BCEL: BcellWH2_00392(tagF)
MARF: CJ739_1496
TME: Tmel_1073
METC: MTCT_0308
MWO: MWSIV6_0753(tagB) MWSIV6_0756(tarL)
METE: tca_00321(tagF_1) tca_00334(tagF_3)
MOL: YLM1_1633
METH: MBMB1_1099
PHO: PH0397(PH0397)
TSI: TSIB_2020
HALL: LC1Hm_0847(tagB)
ABI: Aboo_0707
 » show all
Reference
1  [PMID:14245357]
  Authors
BURGER MM, GLASER L.
  Title
THE SYNTHESIS OF TEICHOIC ACIDS. I. POLYGLYCEROPHOSPHATE.
  Journal
J Biol Chem 239:3168-77 (1964)
Reference
2  [PMID:12637499]
  Authors
Schertzer JW, Brown ED
  Title
Purified, recombinant TagF protein from Bacillus subtilis 168 catalyzes the polymerization of glycerol phosphate onto a membrane acceptor in vitro.
  Journal
J Biol Chem 278:18002-7 (2003)
DOI:10.1074/jbc.M300706200
  Sequence
[bsu:BSU35720]
Reference
3  [PMID:16141206]
  Authors
Schertzer JW, Bhavsar AP, Brown ED
  Title
Two conserved histidine residues are critical to the function of the TagF-like family of enzymes.
  Journal
J Biol Chem 280:36683-90 (2005)
DOI:10.1074/jbc.M507153200
  Sequence
[bsu:BSU35720]
Reference
4  [PMID:18465758]
  Authors
Pereira MP, Schertzer JW, D'Elia MA, Koteva KP, Hughes DW, Wright GD, Brown ED
  Title
The wall teichoic acid polymerase TagF efficiently synthesizes poly(glycerol phosphate) on the TagB product lipid III.
  Journal
Chembiochem 9:1385-90 (2008)
DOI:10.1002/cbic.200800026
  Sequence
[bsu:BSU35720]
Reference
5  [PMID:19520862]
  Authors
Sewell EW, Pereira MP, Brown ED
  Title
The wall teichoic acid polymerase TagF is non-processive in vitro and amenable to study using steady state kinetic analysis.
  Journal
J Biol Chem 284:21132-8 (2009)
DOI:10.1074/jbc.M109.010215
  Sequence
[bsu:BSU35720]
Reference
6  [PMID:20400947]
  Authors
Lovering AL, Lin LY, Sewell EW, Spreter T, Brown ED, Strynadka NC
  Title
Structure of the bacterial teichoic acid polymerase TagF provides insights into membrane association and catalysis.
  Journal
Nat Struct Mol Biol 17:582-9 (2010)
DOI:10.1038/nsmb.1819
  Sequence
[ser:SERP1960]
Reference
7  [PMID:21035733]
  Authors
Brown S, Meredith T, Swoboda J, Walker S
  Title
Staphylococcus aureus and Bacillus subtilis W23 make polyribitol wall teichoic acids using different enzymatic pathways.
  Journal
Chem Biol 17:1101-10 (2010)
DOI:10.1016/j.chembiol.2010.07.017
Other DBs
ExplorEnz - The Enzyme Database: 2.7.8.12
IUBMB Enzyme Nomenclature: 2.7.8.12
ExPASy - ENZYME nomenclature database: 2.7.8.12
BRENDA, the Enzyme Database: 2.7.8.12
CAS: 9076-71-5

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