KEGG   ENZYME: 2.7.8.12Help
Entry
EC 2.7.8.12                 Enzyme                                 

Name
teichoic acid poly(glycerol phosphate) polymerase;
teichoic-acid synthase;
cytidine diphosphoglycerol glycerophosphotransferase;
poly(glycerol phosphate) polymerase;
teichoic acid glycerol transferase;
glycerophosphate synthetase;
CGPTase;
CDP-glycerol glycerophosphotransferase (ambiguous);
Tag polymerase;
CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase;
tagF (gene name);
tarF (gene name) (ambiguous)
Class
Transferases;
Transferring phosphorus-containing groups;
Transferases for other substituted phosphate groups
BRITE hierarchy
Sysname
CDP-glycerol:4-O-[(2R)-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol glycerophosphotransferase
Reaction(IUBMB)
n CDP-glycerol + 4-O-[(2R)-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = n CMP + 4-O-{poly[(2R)-glycerophospho]-(2R)-glycerophospho}-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol [RN:R04042]
Reaction(KEGG)
Substrate
CDP-glycerol [CPD:C00513];
4-O-[(2R)-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Product
CMP [CPD:C00055];
4-O-{poly[(2R)-glycerophospho]-(2R)-glycerophospho}-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Comment
Involved in the biosynthesis of poly glycerol phosphate teichoic acids in bacterial cell walls. This enzyme adds 30--50 glycerol units to the linker molecule, but only after it has been primed with the first glycerol unit by EC 2.7.8.44, teichoic acid poly(glycerol phosphate) primase. cf. EC 2.7.8.45, teichoic acid glycerol-phosphate transferase.
History
EC 2.7.8.12 created 1972, modified 1982, modified 2017
Orthology
K09809  CDP-glycerol glycerophosphotransferase
Genes
ECC: c3694
CBRA: A6J81_12080
IZH: FEM41_21455
ANS: ArsFIN_07500(tagB)
HAV: AT03_13925
APA: APP7_1639
APOR: DDU33_02495
AIO: EXH44_04325
BTO: WQG_4240
BTRE: F542_17720
BTRH: F543_19570
PPR: PBPRA2712(TAGF)
CJI: CJSA_1365(tagF)
CJER: H730_08190
CJQ: UC78_1373(tagF)
CLA: CLA_0297
CCUN: CCUN_1310
CLX: CLAN_0120
DDE: Dde_0439
PSF: PSE_0948
BSU: BSU35720(tagF)
BSR: I33_3701
BSL: A7A1_1147
BSH: BSU6051_35720(tagF)
BSUT: BSUB_03805(tagF)
BSUL: BSUA_03805(tagF)
BSUS: Q433_19575
BSS: BSUW23_17545(tarF)
BST: GYO_3927 GYO_3928(tarF)
BSQ: B657_35720(tagF)
BSX: C663_3464(tarF)
BLI: BL02463(tagF)
BLD: BLi03817(tagF)
BAQ: BACAU_3315(tagF)
BYA: BANAU_3474(tagF)
BAMP: B938_16870
BAML: BAM5036_3208(tagF)
BAMA: RBAU_3424(tagF)
BAMN: BASU_3202(tagF)
BAMB: BAPNAU_3482(tagF)
BAMT: AJ82_18550
BAMY: V529_35530(tagF)
BMP: NG74_03461(tagF)
BAO: BAMF_3411(tagF)
BAZ: BAMTA208_18075(tagF)
BQL: LL3_03706(tagF)
BXH: BAXH7_03695(tagF)
BQY: MUS_3913(tagF)
BAMI: KSO_002645
BAMC: U471_34120
BAMF: U722_17615
BCL: ABC3103
BPUM: BW16_16290
BCK: BCO26_0719(tagF)
BAG: Bcoa_0456
BCOA: BF29_3252(tagF)
BJS: MY9_3625
BACW: QR42_16225
BACP: SB24_12270
BACB: OY17_00105
BACY: QF06_16270
BACL: BS34A_38850(tagF)
BALM: BsLM_3588
GEA: GARCT_03211(tagF_1) GARCT_03217(tagF_2)
VPN: A21D_00861(tagF)
SEP: SE0025
SER: SERP1960(tagF)
SEPP: SEB_01955
SEPS: DP17_896(tagF)
SHA: SH2250(tagF)
SHH: ShL2_02098(tarF_3)
SSP: SSP0204
SCA: SCA_2150
SLN: SLUG_21530(tagF)
SDT: SPSE_0280(tagF1) SPSE_0281(tagF2)
SPAS: STP1_0860
SHU: SHYC_01830(tagF-1)
SCAP: AYP1020_1552(tagF_2)
SSCH: LH95_01695
SSCZ: RN70_02035
SAGQ: EP23_08270
LMOG: BN389_11160(tagF)
LMP: MUO_05685
LMOL: LMOL312_1083(tagF)
LMOX: AX24_02835
LMQ: LMM7_1113
LML: lmo4a_1091(tagF)
LMON: LMOSLCC2376_1054(tagF)
LMOO: LMOSLCC2378_1100(tagF)
LMOA: LMOATCC19117_1105(tagF)
LMOK: CQ02_05645
LIN: lin1074
LWE: lwe1064
LIV: LIV_1030
LGZ: NCTC10812_01708(tagF_4)
PSAB: PSAB_18505
JEO: JMA_33880
KZO: NCTC404_00412(tagF_1)
LLA: L151480(tagF)
LLK: LLKF_0955(tagF)
LLT: CVCAS_0881(tagZ) CVCAS_0891(tagF)
LLS: lilo_0875(tagF)
LLM: llmg_1604(tagF)
LLW: kw2_0920(tagF)
LLJ: LG36_1398(tagF)
LPL: lp_0268(tagF1)
LPT: zj316_0467(tagF1) zj316_1211(tagB) zj316_2p10
LPS: LPST_C0952(tagB)
LPZ: Lp16_0237
LSA: LCA_1576(tagB)
LBR: LVIS_1563
LBN: LBUCD034_1143(tagf1) LBUCD034_1700(tagf3)
PPE: PEPE_1603
PCE: PECL_1254
EFA: EF2493
MPX: MPD5_1115
THL: TEH_16260(tagF)
LCI: LCK_01329(tagZ)
LKI: LKI_07870
LGS: LEGAS_0531(tagF)
CPE: CPE0482
ESR: ES1_01610
ESU: EUS_19710
RCH: RUM_01030
BHU: bhn_I2291
CCT: CC1_07490
BPRO: PMF13cell1_04957(tagF_1) PMF13cell1_04959(tagF_2) PMF13cell1_04966(tagF_3)
CPY: Cphy_0301
ERH: ERH_0432
SCO: SCO2590(SCC123.28)
SMA: SAVERM_5079(tagF)
SGR: SGR_4545
SCT: SCAT_4238(tagF)
SBH: SBI_06508
SVE: SVEN_2375
STRP: F750_2810 F750_2811(rodC) F750_2815(rodC)
STRM: M444_12660
SRW: TUE45_03582(tagF_4)
SLE: sle_16850(sle_16850) sle_42870(sle_42870) sle_46440(sle_46440)
SMAL: SMALA_3090
SLAU: SLA_2269
SALJ: SMD11_4229
SLX: SLAV_24610(tagF2) SLAV_33120(tagF3)
LXL: KDY119_02605(tagF)
MOO: BWL13_01667(tagF) BWL13_01669(tarF_1)
BCV: Bcav_1199
IDO: I598_3353(tagF_3)
SERJ: SGUI_1316
BLIN: BLSMQ_3088
KFL: Kfla_2738
TFU: Tfu_2178
NDA: Ndas_5165
NAL: B005_3643
STRR: EKD16_07695(tagF)
TCU: Tcur_0642
GOB: Gobs_1280
KRA: Krad_3986
AFS: AFR_39485
SNA: Snas_0745
TBI: Tbis_0637
BCEL: BcellWH2_00392(tagF)
MRU: mru_2191
MOL: YLM1_1633
 » show all
Taxonomy
Reference
1  [PMID:14245357]
  Authors
BURGER MM, GLASER L.
  Title
THE SYNTHESIS OF TEICHOIC ACIDS. I. POLYGLYCEROPHOSPHATE.
  Journal
J Biol Chem 239:3168-77 (1964)
Reference
2  [PMID:12637499]
  Authors
Schertzer JW, Brown ED
  Title
Purified, recombinant TagF protein from Bacillus subtilis 168 catalyzes the polymerization of glycerol phosphate onto a membrane acceptor in vitro.
  Journal
J Biol Chem 278:18002-7 (2003)
DOI:10.1074/jbc.M300706200
  Sequence
[bsu:BSU35720]
Reference
3  [PMID:16141206]
  Authors
Schertzer JW, Bhavsar AP, Brown ED
  Title
Two conserved histidine residues are critical to the function of the TagF-like family of enzymes.
  Journal
J Biol Chem 280:36683-90 (2005)
DOI:10.1074/jbc.M507153200
  Sequence
[bsu:BSU35720]
Reference
4  [PMID:18465758]
  Authors
Pereira MP, Schertzer JW, D'Elia MA, Koteva KP, Hughes DW, Wright GD, Brown ED
  Title
The wall teichoic acid polymerase TagF efficiently synthesizes poly(glycerol phosphate) on the TagB product lipid III.
  Journal
Chembiochem 9:1385-90 (2008)
DOI:10.1002/cbic.200800026
  Sequence
[bsu:BSU35720]
Reference
5  [PMID:19520862]
  Authors
Sewell EW, Pereira MP, Brown ED
  Title
The wall teichoic acid polymerase TagF is non-processive in vitro and amenable to study using steady state kinetic analysis.
  Journal
J Biol Chem 284:21132-8 (2009)
DOI:10.1074/jbc.M109.010215
  Sequence
[bsu:BSU35720]
Reference
6  [PMID:20400947]
  Authors
Lovering AL, Lin LY, Sewell EW, Spreter T, Brown ED, Strynadka NC
  Title
Structure of the bacterial teichoic acid polymerase TagF provides insights into membrane association and catalysis.
  Journal
Nat Struct Mol Biol 17:582-9 (2010)
DOI:10.1038/nsmb.1819
  Sequence
[ser:SERP1960]
Reference
7  [PMID:21035733]
  Authors
Brown S, Meredith T, Swoboda J, Walker S
  Title
Staphylococcus aureus and Bacillus subtilis W23 make polyribitol wall teichoic acids using different enzymatic pathways.
  Journal
Chem Biol 17:1101-10 (2010)
DOI:10.1016/j.chembiol.2010.07.017
Other DBs
ExplorEnz - The Enzyme Database: 2.7.8.12
IUBMB Enzyme Nomenclature: 2.7.8.12
ExPASy - ENZYME nomenclature database: 2.7.8.12
BRENDA, the Enzyme Database: 2.7.8.12
CAS: 9076-71-5

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