KEGG ENZYME: 2.7.8.42

ENZYME: 2.7.8.42

Entry

EC 2.7.8.42                 Enzyme

Name

Kdo2-lipid A phosphoethanolamine 7''-transferase;
eptB (gene name)

Class

Transferases;
Transferring phosphorus-containing groups;
Transferases for other substituted phosphate groups

Sysname

diacylphosphatidylethanolamine:alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid-A 7''-phosphoethanolaminetransferase

Reaction(IUBMB)

(1) diacylphosphatidylethanolamine + alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A = diacylglycerol + 7-O-[2-aminoethoxy(hydroxy)phosphoryl]-alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A [RN:R11204];
(2) diacylphosphatidylethanolamine + alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid IVA = diacylglycerol + 7-O-[2-aminoethoxy(hydroxy)phosphoryl]-alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid IVA [RN:R11205]

Reaction(KEGG)

R11204 R11205

Substrate

diacylphosphatidylethanolamine [CPD:C00350];
alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A [CPD:C06026];
alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid IVA [CPD:C06025]

Product

diacyglycerol [CPD:C00641];
7-O-[2-aminoethoxy(hydroxy)phosphoryl]-alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid A [CPD:C21173];
7-O-[2-aminoethoxy(hydroxy)phosphoryl]-alpha-D-Kdo-(2->4)-alpha-D-Kdo-(2->6)-lipid IVA [CPD:C21174]

Comment

The enzyme has been characterized from the bacterium Escherichia coli. It is activated by Ca2+ ions and is silenced by the sRNA MgrR.

History

EC 2.7.8.42 created 2015

Pathway

ec00540

Lipopolysaccharide biosynthesis

Orthology

K12975

KDO II ethanolaminephosphotransferase

Genes

ECO:	b3546(eptB)

ECJ:	JW5660(eptB)

ECD:	ECDH10B_3725(eptB)

EBW:	BWG_3234(eptB)

ECOK:

ECMDS42_2983(eptB)

ECE:	Z4964(yhjW)

ECS:	ECs_4425(eptB)

ECF:	ECH74115_4916

ETW:	ECSP_4537(eptB)

ELX:	CDCO157_4163

EOI:	ECO111_4361(eptB)

EOJ:	ECO26_5060(eptB)

EOH:	ECO103_4693(eptB)

ECOO:

ECRM13514_4536(yhjW)

ECOH:

ECRM13516_4337(yhjW)

ESL:

O3K_01155

ESO:

O3O_24520

ESM:

O3M_01185

ECK:	EC55989_3997(eptB)

ECG:	E2348C_3793(eptB)

EOK:	G2583_4283(eptB)

ELR:	ECO55CA74_20500

ELH:

ETEC_3790

ECW:	EcE24377A_4041

EUN:	UMNK88_4329

ECP:

ECP_3648

ENA:	ECNA114_3697(yhjW)

ECOS:

EC958_3952(yhjW)

ECV:	APECO1_2902(yhjW)

ECOA:

APECO78_21540

ECX:	EcHS_A3747

ECM:	EcSMS35_3867

ECY:

ECSE_3819

ECR:	ECIAI1_3705(eptB)

ECQ:	ECED1_4224(eptB)

EUM:	ECUMN_4058(eptB)

ECT:	ECIAI39_4054(eptB)

EOC:	CE10_4098(eptB)

EBR:	ECB_03396(yhjW)

EBL:	ECD_03396(eptB)

EBE:	B21_03347(eptB)

EBD:

ECBD_0191

ECI:	UTI89_C4086(yhjW)

EIH:	ECOK1_3992

ECZ:	ECS88_3965(eptB)

ECC:	c4364(yhjW)

ELO:	EC042_3852(eptb)

ELN:	NRG857_17630

ESE:

ECSF_3381

ECL:	EcolC_0170

EKO:	EKO11_0187

EKF:	KO11_05020(eptB)

EAB:	ECABU_c39880(yhjW)

EDH:	EcDH1_0166

EDJ:	ECDH1ME8569_3425(eptB)

ELU:	UM146_17900

ELW:	ECW_m3813(eptB)

ELL:	WFL_18620(eptB)

ELC:	i14_4032(yhjW)

ELD:	i02_4032(yhjW)

ELP:	P12B_c3675(eptB)

ELF:	LF82_0575(eptB)

ECOL:

LY180_18190

ECOI:

ECOPMV1_03884(eptB)

ECOJ:

P423_19745

EFE:	EFER_3543(yhjW)

EAL:	EAKF1_ch2402

EMA:	C1192_15755

ESZ:	FEM44_08910(eptB)

STY:	STY4162(yhjW)

STT:	t3879(yhjW)

SEX:	STBHUCCB_40950

SENT:

TY21A_19715

STM:	STM3635(yhjW)

SEO:	STM14_4382(yhjW)

SEV:	STMMW_36251

SEY:	SL1344_3600(yhjW)

SEM:	STMDT12_C36920

SEJ:	STMUK_3622(yhjW)

SEB:	STM474_3807(yhjW)

SEF:	UMN798_3946(yhjW)

SETU:

STU288_18365

SETC:

CFSAN001921_22270

SENR:

STMDT2_35201(yhjW)

SEND:

DT104_36201(yhjW)

SENI:

CY43_18875

SEEN:

SE451236_02740

SEK:	SSPA3259a(yhjW)

SPQ:	SPAB_04518

SEI:	SPC_3713(yhjW)

SEC:	SCH_3567(yhjW)

SEH:	SeHA_C3957

SHB:

SU5_04111

SENH:

CFSAN002069_13865

SEEH:

SEEH1578_04260

SEE:	SNSL254_A3913

SENN:

SN31241_2490

SEW:	SeSA_A3835

SEA:	SeAg_B3848

SENS:

Q786_17755

SED:

SeD_A4017

SEG:	SG3799(yhjW)

SEL:

SPUL_3932

SEGA:

SPUCDC_3918

SET:	SEN3458(yhjW)

SENA:

AU38_17655

SENO:

AU37_17845

SENV:

AU39_17850

SENQ:

AU40_19880

SENL:

IY59_18290

SENJ:

CFSAN001992_15455

SEEC:

CFSAN002050_25145

SEEB:

SEEB0189_001705

SEEP:

I137_18860

SENB:

BN855_37170

SENE:

IA1_17640

SENC:

SEET0819_01905

SES:	SARI_03999

SBG:	SBG_3228(yhjW)

SBZ:

A464_3716

SBV:	N643_16055

SALZ:

EOS98_01105

SFL:	SF3580(yhjW)

SFX:	S4189(yhjW)

SFV:	SFV_3541(yhjW)

SFE:	SFxv_3904(eptB)

SFN:

SFy_5102

SFS:

SFyv_5174

SFT:	NCTC1_03871(eptB)

SSN:	SSON_3844(yhjW)

SBO:	SBO_3545(yhjW)

SBC:	SbBS512_E3983

SHQ:	A0259_21545

ENC:

ECL_00228

ENL:	A3UG_01155

ECLG:

EC036_02190

ECLE:

ECNIH2_01780

ECLN:

ECNIH4_01100

ECLI:

ECNIH5_01325

ECLX:

LI66_01170

ECLY:

LI62_01580

ECLZ:

LI64_01305

ECLO:

ENC_01530

EHM:	AB284_00935

EXF:	BFV63_01155

ECLA:

ECNIH3_01320

ECLC:

ECR091_01320

EAU:	DI57_17570

EKB:	BFV64_00845

ENO:	ECENHK_01090

EEC:	EcWSU1_00196(eptB)

ELG:	BH714_16125

ECAN:

CWI88_01075

ERN:	BFV67_00910

ECLS:

LI67_001885

ECHG:

FY206_01050(eptB)

ESH:	C1N69_00965

ENT:	Ent638_0178

EAS:	Entas_0201

ENR:	H650_14150

ENX:	NI40_000875

ENF:	AKI40_0189

LNI:	CWR52_11575

EBG:	FAI37_05635(eptB)

END:	A4308_08080

ENZ:	G0034_00875(eptB)

ENS:	HWQ15_09485(eptB)

ENK:	LOC22_11995(eptB)

EHU:	D5067_0022195(eptB)

EMOR:

L6Y89_00990(eptB)

ENB:	ELK40_01700

ESA:

ESA_04188

CSK:	ES15_0157(eptB)

CSZ:	CSSP291_19410

CSJ:	CSK29544_01109

CCON:

AFK62_19185

CDM:	AFK67_19820

CSI:	P262_00236

CMJ:	AFK66_000815

CUI:	AFK65_18915

CMW:	AFK63_19435

CTU:	CTU_40480(yhjW)

KPN:	KPN_03904(yhjW)

KPU:	KP1_5249(yhjW)

KPM:	KPHS_50620

KPP:

A79E_0212

KPH:	KPNIH24_25225

KPZ:	KPNIH27_24140

KPV:	KPNIH29_25070

KPW:	KPNIH30_25530

KPY:	KPNIH31_24575

KPG:	KPNIH32_26400

KPC:	KPNIH10_25190

KPQ:	KPR0928_24710

KPT:	VK055_3565

KPO:	KPN2242_22545

KPR:	KPR_5012(eptB)

KPJ:

N559_0247

KPI:	D364_19960

KPA:	KPNJ1_00219

KPS:	KPNJ2_00219

KPX:	PMK1_01405(eptB)

KPB:	FH42_15855

KPNE:

KU54_001065

KPNU:

LI86_01060

KPNK:

BN49_0212(eptB)

KVA:

Kvar_0199

KPE:

KPK_0202

KVD:	KR75_07080

KVQ:	SP68_13680

KOX:

KOX_05505

KOE:

A225_5571

KOY:	J415_04250

KOM:	HR38_03985

KMI:	VW41_23140

KOK:	KONIH1_28035

KOC:	AB185_08365

KQU:	AVR78_03420

EAE:

EAE_06005

EAR:

CCG32801

KQV:	B8P98_01140

KLL:	BJF97_27670

KLW:	DA718_01235(eptB)

KAR:	LGL98_01070(eptB)

KGR:	JJJ10_01145(eptB)

KPAS:

LUW96_05440(eptB)

KLC:	K7H21_01085(eptB)

ROR:	RORB6_19810

RON:	TE10_01155

RPLN:

B1209_00990

RAO:	DSD31_01130

RTG:	NCTC13098_00239(eptB)

REE:	electrica_00201(eptB)

CRO:	ROD_42621(eptb)

CKO:

CKO_04996

CFD:	CFNIH1_05330

CBRA:

A6J81_18235

CWE:	CO701_23085

CYO:	CD187_00945

CPOT:

FOB25_12295(eptB)

CFQ:	C2U38_00155

CSED:

JY391_00935(eptB)

CAMA:

F384_19225

CAF:	AL524_01985

CIF:	AL515_00270

CFAR:

CI104_00950

CIR:	C2U53_08615

CIE:	AN232_05470

CPAR:

CUC49_18285

CTEL:

GBC03_03395(eptB)

CITZ:

E4Z61_17995(eptB)

CARS:

E1B03_25180(eptB)

EBT:	EBL_c01360(yhjW)

CNT:	JT31_12840

CEM:	LH23_04870

CEN:	LH86_04820

CLAP:

NCTC11466_04705(eptB)

PGE:	LG71_01955

ESC:	Entcl_0198

KLE:	AO703_20430

KSA:	C813_25785

KOR:	AWR26_00885(eptB)

KRD:	A3780_23840

KCO:	BWI95_08780

KOT:	EH164_00860

KPSE:

IP581_22230(eptB)

KOB:	HF650_00980(eptB)

KOO:	O9K67_00940(eptB)

KIE:	NCTC12125_04013(eptB)

KAS:	KATP_02060

KLU:	K7B04_06460(eptB)

LAX:	APT61_21300

LEI:	C2U54_05815

LEH:	C3F35_10775

LEE:	DVA44_00865

LER:	GNG29_00835(eptB)

LEA:	GNG26_00865(eptB)

LPNU:

KQ929_19665(eptB)

LAZ:	A8A57_01095

LEF:	LJPFL01_0183

LEW:	DAI21_03420

BUF:	D8682_15850

BAGE:

BADSM9389_00370

BFT:	MNO13_00370(eptB)

SYM:	K6K13_20915(eptB)

METY:

MRY16398_02070

AHN:	NCTC12129_00150(yhjW)

ASUB:

NLZ15_00660(eptB)

YRE:	HEC60_17050(eptB)

SGOE:

A8O29_001510(eptB)

KIN:	AB182_26495

PDZ:	HHA33_03205(eptB)

IZH:	FEM41_04390(eptB)

PSGC:

G163CM_37920(eptB)

SCOL:

KFZ77_00770(eptB)

PVJ:	LMA04_11605(eptB)

EBF:

D782_0188

EBC:	C2U52_09295

EBU:	CUC76_16630

YPE:	YPO4013(yhjW)

YPK:

y4034

YPH:	YPC_4523(eptB)

YPA:

YPA_4101

YPN:

YPN_3661

YPM:

YP_3372

YPP:	YPDSF_3375

YPG:	YpAngola_A4133(eptB)

YPT:	A1122_05770

YPD:

YPD4_3531

YPX:

YPD8_3537

YPW:

CH59_1965

YPJ:

CH55_2691

YPV:

BZ15_3693

YPL:

CH46_1041

YPS:	YPTB3848(yhjW)

YPO:

BZ17_2733

YPI:	YpsIP31758_4081(eptB)

YPY:

YPK_0080

YPB:

YPTS_4066

YPQ:	DJ40_2543(eptB)

YPU:

BZ21_3206

YPR:

BZ20_2240

YPC:

BZ23_3481

YPF:

BZ19_3339

YEN:	YE4090(yhjW)

YEP:	YE105_C3807

YEY:

Y11_31061

YEL:	LC20_00112

YEW:	CH47_3639(eptB)

YET:	CH48_1540(eptB)

YEF:	FORC2_0085

YEE:	YE5303_39451(yhjW)

YSI:	BF17_07105

YAL:

AT01_2595

YFR:	AW19_3363(eptB)

YIN:

CH53_1651

YKR:

CH54_2427

YRO:

CH64_2488

YRU:

BD65_2069

YRB:	UGYR_10630

YAK:	ACZ76_09365

YMA:	DA391_22635

YHI:	D5F51_21680

YCA:	F0T03_21300(eptB)

YMO:	HRD69_05090(eptB)

YAS:	N0H69_00430(eptB)

YKI:	HRD70_03825(eptB)

SMAR:

SM39_4369(eptB)

SMAC:

SMDB11_4239(eptB)

SMW:	SMWW4_v1c01400(eptB)

SPE:	Spro_0131 Spro_1859

SRR:	SerAS9_0094 SerAS9_1801

SRL:	SOD_c00890(eptB1) SOD_c16840(eptB)

SRY:	M621_00445 M621_09315

SPLY:

Q5A_000535(eptB_1) Q5A_009195(eptB_2)

SRS:	SerAS12_0094 SerAS12_1801

SRA:	SerAS13_0093 SerAS13_1802

SMAF:

D781_0097

SLQ:	M495_00470 M495_08690

SERF:

L085_05075

SERS:

SERRSCBI_00565

SFW:	WN53_08990

SFG:	AV650_01935

SRZ:	AXX16_0426

SERM:

CLM71_00580

SQU:	E4343_06865(eptB) E4343_15640(eptB)

SFJ:	SAMEA4384070_0096(eptB_1) SAMEA4384070_1889(eptB_2)

SOF:	NCTC11214_01877(eptB)

SSUR:

ATE40_021380

SURI:

J0X03_23380(eptB)

SRHZ:

FO014_09425(eptB)

SENP:

KHA73_09315(eptB) KHA73_22625(eptB)

SNEM:

NLX84_00400(eptB)

RAH:	Rahaq_0082 Rahaq_1381

RACE:

JHW33_01365(eptB) JHW33_17020(eptB)

RAQ:	Rahaq2_0101 Rahaq2_1444

RAA:	Q7S_00400 Q7S_06665

ROX:	BV494_02230 BV494_16270

RVC:	J9880_08185(eptB) J9880_14840(eptB)

GQU:	AWC35_10925

FSM:	CCS41_08475

EAME:

GXP68_00380(eptB) GXP68_14165(eptB)

RBAD:

H2866_07500(eptB) H2866_14260(eptB)

RCB:	O1V66_17935(eptB)

ECA:

ECA0044

PATR:

EV46_00225

PATO:

GZ59_00480

PCT:

PC1_4207

PCC:	PCC21_000290

PCV:	BCS7_21265

PWA:	Pecwa_4490

PPAR:

A8F97_19000

PEC:

W5S_4672

PWS:	A7983_07985

PPOA:

BJK05_16725

PBRA:

B5S52_21790

PPUJ:

E2566_21345(eptB)

PARI:

I2D83_21795(eptB)

PAQU:

DMB82_0020170(eptB)

PVZ:	OA04_00480(eptB)

PPAV:

LOZ86_00935(eptB)

PQU:	IG609_019935(eptB)

PPOO:

LW347_21385(eptB)

PCAC:

OI450_18160(eptB)

PACB:

M9782_02480(eptB)

DDD:	Dda3937_01131(eptB)

DZE:	Dd1591_4143

DSO:	A4U42_08590

CED:	LH89_12850

DFN:	CVE23_22040

DDQ:

DDI_4189

DAQ:	DAQ1742_00044(eptB)

BGJ:	AWC36_18150

BRB:	EH207_17530

BNG:	EH206_22250

BIZ:	HC231_23185(eptB)

LBQ:	CKQ53_04835

LPOP:

I6N93_16600(eptB)

SOD:	Sant_0121(eptB)

EAM:	EAMY_3617(yhjW)

EAY:

EAM_3396

ETA:

ETA_34000

EPY:

EpC_36110

EPR:	EPYR_03887(yhjW)

EBI:	EbC_22590 EbC_44110

ERJ:	EJP617_10880

EPE:	CI789_19220

ERWI:

GN242_00420(eptB)

ETP:	LU633_12605(eptB)

ERP:	LJN55_00515(eptB)

EGE:	EM595_3372(eptB)

PAM:	PANA_0120(yhjW)

PLF:	PANA5342_4307(yhjW)

PAJ:	PAJ_3281(yhjW)

PAQ:	PAGR_g4156

PVA:	Pvag_3345(yhjW)

PAGG:

AL522_04865

PAO:	Pat9b_0106

KLN:	LH22_01755

PANT:

PSNIH1_06140

PANP:

PSNIH2_17940

PAGC:

BEE12_07245

PSTW:

DSJ_22340

PALH:

B1H58_06880

PANS:

FCN45_00535(eptB) FCN45_20435(eptB)

PEY:	EE896_18590(eptB) EE896_19830(eptB)

PDIS:

D8B20_00395 D8B20_20750

PER:	LAC65_00240(eptB)

PJI:	KTJ90_18560(eptB) KTJ90_21445(eptB)

PANO:

OJ965_20385(eptB)

PGZ:	C2E15_00810

PCD:	C2E16_00950

MINT:

C7M51_03657(eptB)

MTHI:

C7M52_03366(eptB)

TPTY:

NCTC11468_03553(eptB)

KPIE:

N5580_17645(eptB)

PTT:	VY86_10840

PMR:

PMI3576

PMIB:

BB2000_0018

PVG:	CRN77_01920

PHAU:

PH4a_09265

PROT:

BTA34_00875

PCOL:

F1325_02195(eptB)

PCIB:

F9282_02065(eptB)

PPEE:

I6G31_07205(eptB)

PHEI:

NCTC12003_02602(eptB)

MMK:

MU9_3457

MWI:	MNY66_00810(eptB)

HAV:	AT03_21265

HPAR:

AL518_05505

OPO:	DSM2777_04155

LPV:	HYN51_06725

PRAG:

EKN56_15365(eptB)

LRI:	NCTC12151_02063(eptB)

CEK:	D0B88_17795

NEL:	NELON_00405

ECOR:

SAMEA4412678_0072(eptB)

LHK:

LHK_02964

AZA:

AZKH_0150

SUN:

SUN_1948

» show all

Reference

1 [PMID:11042192]

Authors

Kanipes MI, Lin S, Cotter RJ, Raetz CR

Title

Ca2+-induced phosphoethanolamine transfer to the outer 3-deoxy-D-manno-octulosonic acid moiety of Escherichia coli lipopolysaccharide. A novel membrane enzyme dependent upon phosphatidylethanolamine.

Journal

J Biol Chem 276:1156-63 (2001)
DOI:10.1074/jbc.M009019200

Reference

2 [PMID:15795227]

Authors

Reynolds CM, Kalb SR, Cotter RJ, Raetz CR

Title

A phosphoethanolamine transferase specific for the outer 3-deoxy-D-manno-octulosonic acid residue of Escherichia coli lipopolysaccharide. Identification of the eptB gene and Ca2+ hypersensitivity of an eptB deletion mutant.

Journal

J Biol Chem 280:21202-11 (2005)
DOI:10.1074/jbc.M500964200

Sequence

[eco:b3546]

Reference

3 [PMID:23659637]

Authors

Moon K, Six DA, Lee HJ, Raetz CR, Gottesman S

Title

Complex transcriptional and post-transcriptional regulation of an enzyme for lipopolysaccharide modification.

Journal

Mol Microbiol 89:52-64 (2013)
DOI:10.1111/mmi.12257