KEGG   ENZYME: 2.7.9.6
Entry
EC 2.7.9.6                  Enzyme                                 

Name
rifampicin phosphotransferase;
rifampin phosphotransferase;
RPH
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with paired acceptors
Sysname
ATP:rifampicin, water 21-O-phosphotransferase
Reaction(IUBMB)
ATP + rifampicin + H2O = AMP + 21-phosphorifampicin + phosphate [RN:R11964]
Reaction(KEGG)
R11964
Substrate
ATP [CPD:C00002];
rifampicin [CPD:C06688];
H2O [CPD:C00001]
Product
AMP [CPD:C00020];
21-phosphorifampicin [CPD:C21808];
phosphate [CPD:C00009]
Comment
The enzyme, characterized from a diverse collection of Gram-positive bacteria, inactivates the antibiotic rifampicin by phosphorylating it at position 21. The enzyme comprises three domains: two substrate-binding domains (ATP-grasp and rifampicin-binding domains) and a smaller phosphate-carrying L-histidine swivel domain that transits between the spatially distinct substrate-binding sites during catalysis.
History
EC 2.7.9.6 created 2018
Orthology
K22579  rifampicin phosphotransferase
Genes
AQU: 100633750
KLW: DA718_16065
KCO: BWI95_06505
KOT: EH164_03270
DDQ: DDI_2005
PLU: plu4508
PLUM: A4R40_22320
PAY: PAU_04011(ppsA)
PTT: VY86_10490
PVG: CRN77_12550
PHAU: PH4a_15645
PSI: S70_15485
PSX: DR96_2158
MVS: MVIS_1671
METL: U737_14290
MMAI: sS8_1955
TIG: THII_2238
NHL: Nhal_3536
TNI: TVNIR_3777(cmdD_[H])
GAI: IMCC3135_00190(pigC)
HCH: HCH_05508
ABO: ABO_1322
ADI: B5T_02221
APAC: S7S_09130
AXE: P40_10700
CHJ: NCTC10426_00158(ppsA)
SVA: SVA_3748
PSE: NH8B_2490
RPI: Rpic_3805
CGD: CR3_4179(ppsA)
BGP: BGL_2c09640(ppsA)
AXX: ERS451415_02356(ppsA_1) ERS451415_03087(ppsA_3)
CTES: O987_04190
PBH: AAW51_3060(pps)
AZO: azo2486(ppsA2)
AOA: dqs_2639
HCP: HCN_0375
DBA: Dbac_2917
DOL: Dole_1865
DAL: Dalk_5266
DAT: HRM2_42990(ppsA8)
DTO: TOL2_C00330(ppsA)
DALK: DSCA_40090
CCX: COCOR_03165(pps) COCOR_06506(ppsA1)
SCL: sce4177(ppsA1) sce7847(ppsA3) sce7909
HOH: Hoch_3241
PGD: Gal_03947
LAQU: R2C4_20750
MALU: KU6B_29550
BBA: Bd3153(ppsA)
BBAT: Bdt_3083(ppsA)
BBW: BDW_11575
BBAC: EP01_01065
BMX: BMS_0544
AFR: AFE_2412
BSU: BSU18830(pps)
BSH: BSU6051_18830(pps) BSU6051_35190(yvkC)
BSUT: BSUB_02025(pps) BSUB_03749(yvkC)
BSUL: BSUA_02025(pps) BSUA_03749(yvkC)
BSS: BSUW23_17245(yvkC)
BST: GYO_3859
BSO: BSNT_10146(yvkC)
BSQ: B657_18830(pps) B657_35190(yvkC)
BSX: C663_1945(pps) C663_3404(yvkC)
BLD: BLi00634(pps1) BLi02172(pps2) BLi02241(pps3)
BLH: BaLi_c07230(pps1) BaLi_c22490(pps2) BaLi_c23230(pps3)
BAQ: BACAU_0823 BACAU_1707(ppsA) BACAU_3262(yvkC)
BYA: BANAU_0766 BANAU_1861(ppsA) BANAU_3415(yvkC)
BAMA: RBAU_0822 RBAU_1716(pps) RBAU_3370(yvkC)
BAMN: BASU_1696(pps) BASU_3148(yvkC)
BAMY: V529_07930 V529_17030(pps) V529_35010(yvkC)
BMP: NG74_00844(cmdD_1) NG74_01810(ppsA_1) NG74_03400(cmdD_2)
BAO: BAMF_1831(pps) BAMF_3358(yvkC)
BQL: LL3_01922(pps) LL3_03651(yvkC)
BXH: BAXH7_01697(pps) BAXH7_03640(yvkC)
BQY: MUS_0855(ppsA) MUS_2077(pps) MUS_3851(yvkC)
BAN: BA_3116(ppsA)
BAR: GBAA_3116(ppsA)
BAT: BAS2899
BAH: BAMEG_1494(ppsA)
BAI: BAA_3165(ppsA)
BANT: A16_31430
BANR: A16R_31850
BANS: BAPAT_2990
BANV: DJ46_1870
BCE: BC3087
BCA: BCE_3137(ppsA)
BCZ: BCE33L2825(ppsA)
BCR: BCAH187_A3140(ppsA)
BCU: BCAH820_3119(ppsA)
BCG: BCG9842_B2130(ppsA)
BCQ: BCQ_2926(ppsA)
BCX: BCA_3175(ppsA)
BAL: BACI_c30610(ppsA)
BNC: BCN_2943
BCF: bcf_15150
BCER: BCK_19365
BTK: BT9727_2868(ppsA)
BTL: BALH_2779(ppsA)
BTB: BMB171_C2797(ppsA)
BTT: HD73_2868
BTHI: BTK_14220
BTM: MC28_2290
BTI: BTG_03845
BTW: BF38_4274
BWW: bwei_1900(pps)
BMYO: BG05_2916
BMYC: DJ92_810
BPU: BPUM_3151
BPUM: BW16_16875
BPUS: UP12_16395
BMH: BMWSH_3415(pps) BMWSH_3582(yvkC)
BMEG: BG04_4173
BACW: QR42_15860
BACL: BS34A_20860(pps) BS34A_38290(yvkC)
BEO: BEH_08550
BGY: BGLY_0668 BGLY_2363(pps)
BBEV: BBEV_3075(pps)
HLI: HLI_03395
BSE: Bsel_2627
LMOE: BN418_0469
LMOB: BN419_0477
LMOD: LMON_0419
LMOW: AX10_10615
LMOQ: LM6179_0712(pps)
LMR: LMR479A_0421(pps)
LMOM: IJ09_08270
LMOG: BN389_04380(pps)
LMP: MUO_02265
LMOZ: LM1816_01592(ppsA)
LMOX: AX24_14870
LMQ: LMM7_0435(pps)
LMS: LMLG_2941
LMOK: CQ02_02245
LMV: Y193_13690(ppsA)
LIN: lin0432
LSG: lse_0368
LIV: LIV_0343
BBE: BBR47_38090(pps)
PPM: PPSC2_12805(ppsA)
PPO: PPM_2454(ppsA)
PPOL: X809_29155
PPQ: PPSQR21_025160(ppsA)
PPOY: RE92_23965
PMS: KNP414_04423(pps)
PMW: B2K_19970
PLV: ERIC2_c18720(pps)
PSAB: PSAB_12615
PRI: PRIO_3578(pps1) PRIO_3588(pps3) PRIO_6555
PLUT: EI981_14875(ppsA)
PALB: EJC50_20595(ppsA)
ASOC: CB4_02822(ppsA)
PANC: E2636_09015(ppsA)
SGO: SGO_0936 SGO_0940(ppsA)
CRN: CAR_c23280(pps) CAR_c23320(yvkC)
CAC: CA_C0534(pps)
CAE: SMB_G0546(pps) SMB_G0817
CAY: CEA_G0547(pps) CEA_G0812
CBO: CBO2582
CBA: CLB_2523
CBH: CLC_2454
CBY: CLM_2889
CBL: CLK_1967
CBB: CLD_1983
CBI: CLJ_B2812
CBF: CLI_2645
CBM: CBF_2637
CKL: CKL_0066(ppsA) CKL_3749
CSR: Cspa_c20950(ppsA)
CLD: CLSPO_c19550(ppsA)
HSC: HVS_00350(ppsA1) HVS_00370(ppsA2)
CCE: Ccel_2388
EAC: EAL2_808p02480(pps)
DSY: DSY2123(pps) DSY3072 DSY3730(pps) DSY4219(pps)
PTH: PTH_2376(PpsA) PTH_2377
TOC: Toce_1889
PUF: UFO1_4537
PFT: JBW_03750
MANA: MAMMFC1_02616(ppsA)
LPIL: LIP_0991
MBJ: KQ51_00282(ppsA)
MIR: OCQ_16810
MMM: W7S_08675
MSHG: MSG_03742(yvkC)
MGI: Mflv_0493
MTHN: 4412656_00789(ppsA_1) 4412656_02902(ppsA_4) 4412656_03343(ppsA_5)
MTER: 4434518_01742(ppsA_1)
RER: RER_08050
RFA: A3L23_00997(cmdD_1) A3L23_01021(cmdD_2)
RRT: 4535765_01581(ppsA_2)
GRU: GCWB2_01990(cmdD1) GCWB2_02005(cmdD2)
GOM: D7316_04262(pigC)
SMA: SAVERM_3522(ppsA)
SGR: SGR_6947
SGB: WQO_10270
SCB: SCAB_3061
SBH: SBI_08089
SAMB: SAM23877_7140(pps)
SNR: SNOUR_04785(pps)
SLAU: SLA_0353
SALJ: SMD11_5599(pps)
SLX: SLAV_09055(ppsA1)
SGE: DWG14_00454(pigC) DWG14_00731(ppsA_1)
SRJ: SRO_0398
ARR: ARUE_c03840(pps)
ARX: ARZXY2_3510(pps)
ACIT: HPK19_09370(ppsA)
AAU: AAur_0411
SERJ: SGUI_2749
BLIN: BLSMQ_3021
DCO: SAMEA4475696_0328(ppsA)
ACIJ: JS278_00847(ppsA)
NDK: I601_2866(cmdD)
NDA: Ndas_5551
NAL: B005_0348
STRR: EKD16_11290(cmdD)
GOB: Gobs_1069
BSD: BLASA_1739(ppsA2)
AMD: AMED_5423(ppsA)
AMN: RAM_27645
AMM: AMES_5358(ppsA)
AMZ: B737_5358(ppsA)
AOI: AORI_5014(ppsA)
AMYY: YIM_30115(ppsA2)
PDX: Psed_2944
PSEA: WY02_11005
PSEH: XF36_27600
SESP: BN6_41670
SAQ: Sare_3009
MIL: ML5_4711
ACTN: L083_4520
AFS: AFR_16715
TBI: Tbis_2573
BSOL: FSW04_00340(ppsA)
LET: O77CONTIG1_02378(cmdD_2)
MAR: MAE_17610
MPK: VL20_3108
CYT: cce_1632
GLJ: GKIL_2958(pps)
AVA: Ava_C0174
CTHE: Chro_0901
HAU: Haur_1951
TRO: trd_A0070
ATM: ANT_09030
PNL: PNK_2023
PUV: PUV_12540
CAA: Caka_0202
AGL: PYTT_2231
RUL: UC8_01980(ppsA) UC8_02060(cmdD)
GES: VT84_37305(ppsA)
LRS: PX52LOC_05058(ptsP)
AGV: OJF2_69580(ppsA)
TDE: TDE0349
GBA: J421_1816
CPI: Cpin_2960
CHIT: FW415_14380(ppsA)
CHIH: GWR21_29615(ppsA)
PGIN: FRZ67_18140(ppsA)
PEK: FFJ24_019140(ppsA)
MUH: HYN43_013360(ppsA)
MGIN: FRZ54_17690(ppsA)
MGK: FSB76_23105(ppsA)
MRUB: DEO27_013605(ppsA)
MGOS: DIU38_008335(ppsA)
DFE: Dfer_0644
SPIK: EXU85_17795(ppsA)
SPIB: G8759_34505(ppsA)
FAE: FAES_4668(ppsA)
RHOZ: GXP67_01690(ppsA)
CHK: D4L85_30770(ppsA)
CABY: Cabys_1123
NDE: NIDE0497
NMV: NITMOv2_0414(ppsA)
NJA: NSJP_1540
MOX: DAMO_2356
METH: MBMB1_0883
MFC: BRM9_1050
MCUB: MCBB_0337(yvkC)
MARC: AR505_1104
TSI: TSIB_1635
MBAR: MSBR2_1104
MBAK: MSBR3_0152
MAC: MA_2458(ppsA)
METM: MSMTP_0750
MMH: Mmah_1079
RCI: RCIX1188(ppsA-1)
HME: HFX_2676(pps2)
HLA: Hlac_0508
POG: Pogu_1788
PSYT: DSAG12_00137(ppsA_1) DSAG12_03813(ppsA_3)
 » show all
Reference
1  [PMID:24778229]
  Authors
Spanogiannopoulos P, Waglechner N, Koteva K, Wright GD
  Title
A rifamycin inactivating phosphotransferase family shared by environmental and pathogenic bacteria.
  Journal
Proc Natl Acad Sci U S A 111:7102-7 (2014)
DOI:10.1073/pnas.1402358111
  Sequence
Reference
2  [PMID:27103605]
  Authors
Stogios PJ, Cox G, Spanogiannopoulos P, Pillon MC, Waglechner N, Skarina T, Koteva K, Guarne A, Savchenko A, Wright GD
  Title
Rifampin phosphotransferase is an unusual antibiotic resistance kinase.
  Journal
Nat Commun 7:11343 (2016)
DOI:10.1038/ncomms11343
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.7.9.6
IUBMB Enzyme Nomenclature: 2.7.9.6
ExPASy - ENZYME nomenclature database: 2.7.9.6
BRENDA, the Enzyme Database: 2.7.9.6

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