Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with paired acceptors
BRITE hierarchy

ATP:rifampicin, water 21-O-phosphotransferase

ATP + rifampicin + H2O = AMP + 21-phosphorifampicin + phosphate [RN:R11964]

R11964
Reaction

ATP [CPD:C00002];
rifampicin [CPD:C06688];
H2O [CPD:C00001]

The enzyme, characterized from a diverse collection of Gram-positive bacteria, inactivates the antibiotic rifampicin by phosphorylating it at position 21. The enzyme comprises three domains: two substrate-binding domains (ATP-grasp and rifampicin-binding domains) and a smaller phosphate-carrying L-histidine swivel domain that transits between the spatially distinct substrate-binding sites during catalysis.

EC 2.7.9.6 created 2018

1  [PMID:24778229]

Spanogiannopoulos P, Waglechner N, Koteva K, Wright GD

A rifamycin inactivating phosphotransferase family shared by environmental and pathogenic bacteria.

Proc Natl Acad Sci U S A 111:7102-7 (2014)
DOI:10.1073/pnas.1402358111

[ag:AIA08936]

2  [PMID:27103605]

Stogios PJ, Cox G, Spanogiannopoulos P, Pillon MC, Waglechner N, Skarina T, Koteva K, Guarne A, Savchenko A, Wright GD

Rifampin phosphotransferase is an unusual antibiotic resistance kinase.

Nat Commun 7:11343 (2016)
DOI:10.1038/ncomms11343

[lmf:LMOf2365_0430]