KEGG ENZYME: 2.8.2.14

ENZYME: 2.8.2.14

Entry

EC 2.8.2.14 Enzyme

Name

bile-salt sulfotransferase;
BAST I;
bile acid:3'-phosphoadenosine-5'-phosphosulfate sulfotransferase;
bile salt:3'phosphoadenosine-5'-phosphosulfate:sulfotransferase;
bile acid sulfotransferase I;
glycolithocholate sulfotransferase;
3'-phosphoadenylyl-sulfate:glycolithocholate sulfotransferase

Class

Transferases;
Transferring sulfur-containing groups;
Sulfotransferases

Sysname

3'-phosphoadenylyl-sulfate:glycolithocholate sulfonotransferase

Reaction(IUBMB)

(1) 3'-phosphoadenylyl sulfate + glycolithocholate = adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate [RN:R07287];
(2) 3'-phosphoadenylyl sulfate + taurolithocholate = adenosine 3',5'-bisphosphate + taurolithocholate sulfate [RN:R03987]

Reaction(KEGG)

R03987 R07287;
(other) R08977 R08978 R09444

Substrate

3'-phosphoadenylyl sulfate [CPD:C00053];
glycolithocholate [CPD:C15557];
taurolithocholate [CPD:C02592]

Product

adenosine 3',5'-bisphosphate [CPD:C00054];
glycolithocholate 3-sulfate [CPD:C11301];
taurolithocholate sulfate [CPD:C03642]

Comment

The formation of sulfate esters of bile acids is an essential step in the prevention of toxicity by monohydroxy bile acids in many species [3]. This enzyme is both a bile salt and a 3-hydroxysteroid sulfotransferase. In addition to the 5beta-bile acid glycolithocholate, deoxycholate, 3beta-hydroxy-5-cholenoate and dehydroepiandrosterone (3beta-hydroxyandrost-5-en-17-one) also act as substrates [see also EC 2.8.2.2 (alcohol sulfotransferase) and EC 2.8.2.34 (glycochenodeoxycholate sulfotransferase)]. May be identical to EC 2.8.2.2 [3].

History

EC 2.8.2.14 created 1978, modified 2005

Pathway

ec00980

Metabolism of xenobiotics by cytochrome P450

Orthology

K11822

bile-salt sulfotransferase

Genes

HSA:	6822(SULT2A1)

PTR:	456409(SULT2A1)

PPS:	100971784(SULT2A1)

GGO:	101137965(SULT2A1)

PON:	100431471(SULT2A1)

NLE:	100603984(SULT2A1)

MCC:	717600(SULT2A1)

MCF:	102130325(SULT2A1)

CSAB:

103234953(SULT2A1)

RRO:	104673376(SULT2A1)

RBB:	108539899(SULT2A1)

CJC:	100413663(SULT2A1)

SBQ:	101034912 101040745

MMU:	20859(Sult2a1) 434121(Sult2a4) 434264(Sult2a5) 629203(Sult2a3) 638251(Sult2a7) 76971(Sult2a8)

MCAL:

110287694 110297377 110298916 110299139(Sult2a1)

MPAH:

110315086(Sult2a1) 110315385

RNO:	24912(Sult2a1) 361510(Sult2a2)

CGE:	100751331 100751624 100751913 100752375 100752864 100753164 103160274 103160275 113837373

NGI:	103729182 103732419 103732424

HGL:	101704327(Sult2a1) 101704668 101705514

CCAN:

109675537

OCU:	100008598(SULT2A1)

TUP:	102469211 102470067

BTA:	514658 533560(SULT2A1)

BOM:	102268811 102269085

BIU:	109572833 109572834

BBUB:

102395145 102395467

CHX:	102181069 108633179

OAS:	101119842 101120103

SSC:	641359(SULT2A1)

CFR:	102518264(SULT2A1)

CDK:	105098927(SULT2A1)

BACU:

103005028(SULT2A1)

OOR:

105747730

DLE:

111180495

ECB:	100050266 100050329 100050400 100050477(SULT2A1) 100050557 100052843 100052993

EPZ:	103544219 103545059 103555781 103555782 103555784(SULT2A1) 103567536

EAI:	106829389 106838230 106838231 106845150

MYB:	102240460 102254636(SULT2A1)

MYD:	102762349 102762941(SULT2A1)

MNA:	107540047(SULT2A1)

HAI:	109390976(SULT2A1)

DRO:	112312826(SULT2A1) 112313480

PALE:

102890032(SULT2A1)

RAY:	107516041(SULT2A1)

LAV:	100654856 100655145 100655424 104845577

TMU:	101344801 101351802

MDO:	100018596 100018631

SHR:	100920297 105750186

PCW:	110220147 110220161

CRG:

105336931

WIJ:	BWZ20_04230

ALTI:

ALE3EI_0126

» show all

Reference

1 [PMID:831833]

Authors

Chen LJ, Bolt RJ, Admirand WH.

Title

Enzymatic sulfation of bile salts. Partial purification and characterization of an enzyme from rat liver that catalyzes the sulfation of bile salts.

Journal

Biochim Biophys Acta 480:219-27 (1977)
DOI:10.1016/0005-2744(77)90335-7

Reference

2 [PMID:3470420]

Authors

Barnes S, Waldrop R, Crenshaw J, King RJ, Taylor KB.

Title

Evidence for an ordered reaction mechanism for bile salt: 3'phosphoadenosine-5'-phosphosulfate: sulfotransferase from rhesus monkey liver that catalyzes the sulfation of the hepatotoxin glycolithocholate.

Journal

J Lipid Res 27:1111-23 (1986)

Reference

3 [PMID:2754334]

Authors

Barnes S, Buchina ES, King RJ, McBurnett T, Taylor KB.

Title

Bile acid sulfotransferase I from rat liver sulfates bile acids and 3-hydroxy steroids: purification, N-terminal amino acid sequence, and kinetic properties.

Journal

J Lipid Res 30:529-40 (1989)

Reference

4 [PMID:12543708]

Authors

Russell DW

Title

The enzymes, regulation, and genetics of bile acid synthesis.

Journal

Annu Rev Biochem 72:137-74 (2003)
DOI:10.1146/annurev.biochem.72.121801.161712

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