EC                 Enzyme                                 

[heparan sulfate]-glucosamine 3-sulfotransferase 1;
heparin-glucosamine 3-O-sulfotransferase;
3'-phosphoadenylyl-sulfate:heparin-glucosamine 3-O-sulfotransferase;
glucosaminyl 3-O-sulfotransferase;
heparan sulfate D-glucosaminyl 3-O-sulfotransferase;
isoform/isozyme 1 (3-OST-1, HS3ST1);
3'-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine 3-sulfotransferase
Transferring sulfur-containing groups;
3'-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine 3-sulfonotransferase
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate [RN:R05798]
3'-phosphoadenylyl sulfate [CPD:C00053];
[heparan sulfate]-glucosamine [CPD:C17141]
adenosine 3',5'-bisphosphate [CPD:C00054];
[heparan sulfate]-glucosamine 3-sulfate [CPD:C11553]
This enzyme differs from the other [heparan sulfate]-glucosamine 3-sulfotransferases [EC ([heparan sulfate]-glucosamine 3-sulfotransferase 2) and EC ([heparan sulfate]-glucosamine 3-sulfotransferase 3)] by being the most selective for a precursor of the antithrombin-binding site. It has a minimal acceptor sequence of: -> GlcNAc6S-> GlcA-> GlcN2S*+/-6S-> IdoA2S-> GlcN2S-> , the asterisk marking the target (symbols as in {iupac/2carb/38::2-Carb-38}) using +/- to mean the presence or absence of a substituent, and > to separate a predominant structure from a minor one. Thus Glc(N2S > NAc) means a residue of glucosamine where the N carries a sulfo group mainly but occasionally an acetyl group. [1-4]. It can also modify other precursor sequences within heparan sulfate but this action does not create functional antithrombin-binding sites. These precursors are variants of the consensus sequence: -> Glc(N2S > NAc)+/-6S-> GlcA-> GlcN2S*+/-6S-> GlcA > IdoA+/-2S-> Glc(N2S/NAc)+/-6S-> [5]. If the heparan sulfate substrate lacks 2-O-sulfation of GlcA residues, then enzyme specificity is expanded to modify selected glucosamine residues preceded by IdoA as well as GlcA [6].
EC created 1992, modified 2001
ec00534  Glycosaminoglycan biosynthesis - heparan sulfate / heparin
K01024  [heparan sulfate]-glucosamine 3-sulfotransferase 1
K08104  [heparan sulfate]-glucosamine 3-sulfotransferase 5
HSA: 222537(HS3ST5) 9957(HS3ST1)
PTR: 471144(HS3ST1) 744298(HS3ST5)
PPS: 100967734(HS3ST1) 100979280(HS3ST5)
GGO: 101134668(HS3ST1) 101154208(HS3ST5)
PON: 100445561(HS3ST5) 100449950(HS3ST1)
NLE: 100592585(HS3ST5) 100601459(HS3ST1)
MCC: 693684(HS3ST5) 713374(HS3ST1)
MCF: 101865986(HS3ST1) 102141292(HS3ST5)
CSAB: 103240530(HS3ST5) 103246360(HS3ST1)
RRO: 104657111(HS3ST5) 104657730(HS3ST1)
RBB: 108512071(HS3ST5) 108529481(HS3ST1)
CJC: 100392281(HS3ST1) 100402689(HS3ST5)
SBQ: 101038172(HS3ST5) 101043463(HS3ST1)
MMU: 15476(Hs3st1) 319415(Hs3st5)
MCAL: 110295395(Hs3st1) 110303745(Hs3st5)
MPAH: 110326616(Hs3st5) 110330712(Hs3st1)
RNO: 294449(Hs3st5) 84406(Hs3st1)
MUN: 110558382(Hs3st1) 110562327(Hs3st5)
CGE: 100759378(Hs3st1) 100763965(Hs3st5)
NGI: 103728889(Hs3st1) 103733777(Hs3st5)
HGL: 101698728(Hs3st1) 101710328(Hs3st5)
CCAN: 109677593(Hs3st5) 109694636(Hs3st1)
OCU: 100343879(HS3ST1) 100346980(HS3ST5)
TUP: 102468225(HS3ST5) 102481838(HS3ST1)
CFA: 403672(HS3ST1) 481968(HS3ST5)
VVP: 112919834(HS3ST1) 112925192(HS3ST5)
AML: 100478675(HS3ST5) 105241055(HS3ST1)
UMR: 103658356(HS3ST1) 103676972(HS3ST5)
UAH: 113264706(HS3ST5) 113266681(HS3ST1)
ORO: 101364972(HS3ST5) 101380812(HS3ST1)
FCA: 101088412(HS3ST5) 101090103(HS3ST1)
PTG: 102952040(HS3ST5) 102965928(HS3ST1)
PPAD: 109263574(HS3ST5) 109269655(HS3ST1)
AJU: 106968920(HS3ST5) 106975621(HS3ST1)
BTA: 538691(HS3ST1) 540355(HS3ST5)
BOM: 102279879(HS3ST5) 102279923(HS3ST1)
BIU: 109560679(HS3ST1) 109563804(HS3ST5)
BBUB: 102396360(HS3ST5) 102410787(HS3ST1)
CHX: 102169088(HS3ST1) 102176690(HS3ST5)
OAS: 101104909(HS3ST1) 101107448(HS3ST5)
SSC: 100155403(HS3ST5) 100628223(HS3ST1)
CFR: 102503844(HS3ST5) 102515681(HS3ST1)
CDK: 105085126(HS3ST1) 105099225(HS3ST5)
BACU: 103015413(HS3ST1) 103016478(HS3ST5)
LVE: 103068414(HS3ST5) 103089700(HS3ST1)
OOR: 101277155(HS3ST5) 101277987(HS3ST1)
DLE: 111164833(HS3ST5) 111171441(HS3ST1)
PCAD: 102981184(HS3ST1) 102981885(HS3ST5)
ECB: 100056091(HS3ST1) 100066938(HS3ST5)
EPZ: 103561316(HS3ST5)
EAI: 106847041(HS3ST5) 106847606(HS3ST1)
MYB: 102239991(HS3ST5) 102241744(HS3ST1)
MYD: 102756820(HS3ST1) 102773229(HS3ST5)
MNA: 107530094(HS3ST1) 107540725(HS3ST5)
HAI: 109375997(HS3ST1) 109384729(HS3ST5)
DRO: 112297336(HS3ST5) 112316867(HS3ST1)
PALE: 102879059(HS3ST5) 102886681(HS3ST1)
RAY: 107501898(HS3ST1) 107506297(HS3ST5)
MJV: 108385755(HS3ST5) 108408657(HS3ST1)
LAV: 100673247(HS3ST5) 100673479(HS3ST1)
MDO: 100012960(HS3ST1) 100022809(HS3ST5)
SHR: 100917710(HS3ST1) 100926311(HS3ST5)
PCW: 110212282(HS3ST1) 110222937(HS3ST5)
OAA: 100079950(HS3ST5) 100087772(HS3ST1)
GGA: 422840(HS3ST1) 423822(HS3ST1L) 428621(HS3ST5)
MGP: 100541206 100541289(HS3ST5) 100546598(HS3ST1)
CJO: 107311893(HS3ST5) 107314022(HS3ST1) 107315944
NMEL: 110396209(HS3ST5) 110398244(HS3ST1) 110399950
APLA: 101791819(HS3ST5) 101793237(HS3ST1) 101804190
ACYG: 106032639(HS3ST5) 106035306(HS3ST1) 106038121
TGU: 100217958 100221445(HS3ST5) 100221819(HS3ST1)
LSR: 110473696 110484032(HS3ST1) 110484659(HS3ST5)
SCAN: 103813903 103818501(HS3ST5) 103824251(HS3ST1)
GFR: 102031527(HS3ST1) 102038656(HS3ST5) 102043838
FAB: 101809457(HS3ST5) 101814866(HS3ST1) 101816899
PHI: 102102605(HS3ST1) 102104930(HS3ST5) 102109527
PMAJ: 107202537(HS3ST5) 107203500(HS3ST1) 107206970
CCAE: 111927167(HS3ST5) 111928184(HS3ST1) 111931213
CCW: 104690727(HS3ST1) 104691254(HS3ST5) 104696091
ETL: 114062803(HS3ST5) 114063708(HS3ST1) 114071154
FPG: 101919371 101923794(HS3ST5) 101923963(HS3ST1)
FCH: 102053103(HS3ST1) 102054204 102057031(HS3ST5)
CLV: 102091303(HS3ST1) 102094670 102096271(HS3ST5)
EGZ: 104129170 104131984(HS3ST5) 104134473(HS3ST1)
NNI: 104008593(HS3ST5) 104010770(HS3ST1) 104012880
ACUN: 113478652(HS3ST5) 113479378(HS3ST1) 113481620
PADL: 103916288 103918367(HS3ST5) 103920965(HS3ST1)
AAM: 106489636(HS3ST5) 106494700 106498671(HS3ST1)
ASN: 102376753(HS3ST1) 102378310(HS3ST5) 102385617
AMJ: 102560943(HS3ST5) 102573417(HS3ST1L1) 102574400(HS3ST1)
PSS: 102444953 102452315(HS3ST5) 102452655(HS3ST1) 102460581
CMY: 102930016 102930942(HS3ST1) 102936568(HS3ST5) 102947282
CPIC: 101935011 101940499(HS3ST5) 101942391(HS3ST1) 101947422
ACS: 100560134 100562373(hs3st1) 100563170 100566144(hs3st5)
PVT: 110072143 110079727(HS3ST1) 110088773(HS3ST5)
PBI: 103049756(HS3ST1) 103051973 103053046 103058998(HS3ST5)
PMUR: 107287970 107290944(HS3ST1) 107297710(HS3ST5)
TSR: 106540863(HS3ST5) 106548207 106549744(HS3ST1)
PMUA: 114594062(HS3ST5) 114597350 114602851 114604436(HS3ST1)
GJA: 107107831 107118809(HS3ST1) 107123623 107124746(HS3ST5)
XLA: 108696134(XB5817455.L) 108697406(XB5817455.S) 108716820(hs3st5.L) 398971(hs3st1.L) 399064(hs3st1.S)
XTR: 100125092 100145790(hs3st5) 496940(hs3st1)
NPR: 108785216(HS3ST1) 108788983 108789380(HS3ST5)
DRE: 557111(hs3st1l1) 562344(hs3st1l2) 565201(hs3st1)
IPU: 108255327 108255574(hs3st5) 108260334(hs3st1) 108262815(HS3ST1)
PHYP: 113525231(hs3st5) 113528584(hs3st1) 113536887 113543603
AMEX: 103023335(hs3st1) 103029341(hs3st5) 103036825 103039507
TRU: 101061220 101065122 101076430(hs3st1) 101079258(hs3st5)
LCO: 104921922 104923456 104928689(hs3st1) 104932424(hs3st5)
MZE: 101469297 101475274 101475421(hs3st1) 101477610(hs3st5)
ONL: 100690217(hs3st5) 100693479 100694141 100706648(hs3st1)
OLA: 101165781(hs3st5) 101166804(hs3st1) 101169760 101174369
XMA: 102216507(hs3st5) 102220745 102232490 102232809(hs3st1)
XCO: 114137410 114139564(hs3st1) 114154215 114158130(hs3st5)
CVG: 107092361(hs3st5) 107092911(hs3st1) 107097329 107102609
KMR: 108228841 108231121(hs3st1) 108242749(hs3st1l2) 108246557(hs3st1l1)
ALIM: 106510800 106525368(hs3st1) 106535966(hs3st5) 106536957
AOCE: 111575282 111583975 111587138(hs3st5) 111588477(hs3st1)
CSEM: 103380640(hs3st5) 103387477 103390605(hs3st1) 103397870
SDU: 111220657 111227346 111232616(hs3st1) 111240260(hs3st5)
HCQ: 109515940(hs3st1) 109523019
BPEC: 110155521(hs3st5) 110158917(hs3st1) 110161364 110172130
MALB: 109951332 109960951 109966559(hs3st5) 109974538(hs3st1)
ELS: 105007233 105017884(hs3st5) 105025937 105029317(hs3st1)
SFM: 108924148(hs3st1) 108925805 108929368(hs3st5) 108940372
LCM: 102345335(HS3ST5) 102346461(HS3ST1) 102352685 102364972
CMK: 103175792(hs3st1) 103180481 103182576(hs3st5) 103187765
RTP: 109915052 109919900(hs3st1) 109931567 109936180(hs3st5)
BFO: 118409574
DME: Dmel_CG33147(Hs3st-A)
DER: 6547303
DSE: 6609847
DSI: Dsimw501_GD11407(Dsim_GD11407)
DAN: 6493978
DSR: 110178442
DPE: 6590970
DWI: 6638230
DHE: 111604217
DVI: 6627127
MDE: 101898689
LCQ: 111682088
AAG: 5570723
AME: 412956
BIM: 105680199
BTER: 100645554
CCAL: 108632071
OBB: 114871348
SOC: 105207583
MPHA: 105830972
AEC: 105149682
ACEP: 105619000
PBAR: 105432185
VEM: 105565350
HST: 105192616
DQU: 106750173
CFO: 105258568
LHU: 105677087
PGC: 109857918
OBO: 105286868
PCF: 106791895
NVI: 100123415
CSOL: 105368630
MDL: 103578076
TCA: 660198
DPA: 109533375
ATD: 109598304
NVL: 108569938
BMOR: 101740692
BMAN: 114248729
PMAC: 106720254
PRAP: 110991581
HAW: 110369990
TNL: 113505923
API: 100167476
DNX: 107170790
AGS: 114123845
RMD: 113561172
BTAB: 109041313
CLEC: 106674312
FCD: 110844017
PVM: 113815265
TUT: 107367708
DPTE: 113794322
CBR: CBG02583(Cbr-hst-3.1)
BMY: Bm1_42350
TSP: Tsp_02737
ADF: 107354956
AMIL: 114966194
AQU: 100641408
 » show all
1  [PMID:3139669]
Kusche M, Backstrom G, Riesenfeld J, Petitou M, Choay J, Lindahl U.
Biosynthesis of heparin. O-sulfation of the antithrombin-binding region.
J Biol Chem 263:15474-84 (1988)
2  [PMID:8900197]
Shworak NW, Fritze LM, Liu J, Butler LD, Rosenberg RD.
Cell-free synthesis of anticoagulant heparan sulfate reveals a limiting converting activity that modifies an excess precursor pool.
J Biol Chem 271:27063-71 (1996)
3  [PMID:8900198]
Liu J, Shworak NW, Fritze LM, Edelberg JM, Rosenberg RD.
Purification of heparan sulfate D-glucosaminyl 3-O-sulfotransferase.
J Biol Chem 271:27072-82 (1996)
4  [PMID:9346953]
Shworak NW, Liu J, Fritze LM, Schwartz JJ, Zhang L, Logeart D, Rosenberg RD
Molecular cloning and expression of mouse and human cDNAs encoding heparan sulfate D-glucosaminyl 3-O-sulfotransferase.
J Biol Chem 272:28008-19 (1997)
[hsa:9957] [mmu:15476]
5  [PMID:10026187]
Zhang L, Yoshida K, Liu J, Rosenberg RD.
Anticoagulant heparan sulfate precursor structures in F9 embryonal carcinoma cells.
J Biol Chem 274:5681-91 (1999)
6  [PMID:11375390]
Zhang L, Lawrence R, Schwartz JJ, Bai X, Wei G, Esko JD, Rosenberg RD.
The effect of precursor structures on the action of glucosaminyl 3-O-sulfotransferase-1 and the biosynthesis of anticoagulant heparan sulfate.
J Biol Chem 276:28806-13 (2001)
Other DBs
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IUBMB Enzyme Nomenclature:
ExPASy - ENZYME nomenclature database:
BRENDA, the Enzyme Database:
CAS: 183257-54-7

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