KEGG   ENZYME: 2.8.2.33
Entry
EC 2.8.2.33                 Enzyme                                 

Name
N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase;
GalNAc4S-6ST;
CHST15 (gene name);
3'-phosphoadenylyl-sulfate:[dermatan]-4-O-sulfo-N-acetyl-D-galactosamine 6-O-sulfotransferase
Class
Transferases;
Transferring sulfur-containing groups;
Sulfotransferases
Sysname
3'-phosphoadenylyl-sulfate:[dermatan]-4-O-sulfo-N-acetyl-D-galactosamine 6-O-sulfonotransferase
Reaction(IUBMB)
(1) 3'-phospho-5'-adenylyl sulfate + [dermatan]-4-O-sulfo-N-acetyl-D-galactosamine = adenosine 3',5'-bisphosphate + [dermatan]-4,6-di-O-sulfo-N-acetyl-D-galactosamine [RN:R10868];
(2) 3'-phospho-5'-adenylyl sulfate + [chondroitin]-4-O-sulfo-N-acetyl-D-galactosamine = adenosine 3',5'-bisphosphate + [chondroitin]-4,6-di-O-sulfo-N-acetyl-D-galactosamine [RN:R10869]
Reaction(KEGG)
R10868 R10869
Substrate
3'-phospho-5'-adenylyl sulfate [CPD:C00053];
[dermatan]-4-O-sulfo-N-acetyl-D-galactosamine;
[chondroitin]-4-O-sulfo-N-acetyl-D-galactosamine
Product
adenosine 3',5'-bisphosphate [CPD:C00054];
[dermatan]-4,6-di-O-sulfo-N-acetyl-D-galactosamine;
[chondroitin]-4,6-di-O-sulfo-N-acetyl-D-galactosamine
Comment
The enzyme is activated by divalent cations and reduced glutathione. The enzyme from human transfers sulfate to position 6 of both internal residues and non-reducing terminal GalNAc 4-sulfate residues of chondroitin sulfate and dermatan sulfate. Oligosaccharides derived from chondroitin sulfate also serve as acceptors but chondroitin sulfate E, keratan sulfate and heparan sulfate do not. Differs from EC 2.8.2.17, chondroitin 6-sulfotransferase, in being able to use both chondroitin and dermatan as effective substrates
History
EC 2.8.2.33 created 2005, modified 2010
Pathway
ec00532  Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
Orthology
K08106  N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase
Genes
HSA: 51363(CHST15)
PTR: 466249(CHST15)
PPS: 100988573(CHST15)
GGO: 101139112(CHST15)
PON: 100446124(CHST15)
NLE: 100593528(CHST15)
MCC: 693845(CHST15)
MCF: 102140207(CHST15)
CSAB: 103216659(CHST15)
RRO: 104657648(CHST15)
RBB: 108519585(CHST15)
CJC: 100400214(CHST15)
SBQ: 101052289(CHST15)
MMU: 77590(Chst15)
MCAL: 110298310(Chst15)
MPAH: 110322292(Chst15)
RNO: 286974(Chst15)
CGE: 100768085(Chst15)
NGI: 103729255(Chst15)
HGL: 101726691(Chst15)
CCAN: 109691645(Chst15)
OCU: 100343176(CHST15)
TUP: 102470510(CHST15)
CFA: 486928(CHST15)
VVP: 112928825(CHST15)
AML: 100472799(CHST15)
UMR: 103658000(CHST15)
UAH: 113252027(CHST15)
ORO: 101386186(CHST15)
ELK: 111145346
FCA: 101098166(CHST15)
PTG: 102959008(CHST15)
PPAD: 109252307(CHST15)
AJU: 106971732(CHST15)
BTA: 534506(CHST15)
BOM: 102270502(CHST15)
BIU: 109553118(CHST15)
BBUB: 102401618(CHST15)
CHX: 102179262(CHST15)
OAS: 101113976(CHST15)
SSC: 100152393(CHST15)
CFR: 102518619(CHST15)
CDK: 105100278(CHST15)
BACU: 103000709(CHST15)
LVE: 103088154(CHST15)
OOR: 101287863(CHST15)
DLE: 111172221(CHST15)
PCAD: 102980366(CHST15)
ECB: 100057594(CHST15)
EPZ: 103548446(CHST15)
EAI: 106847753(CHST15)
MYB: 102256050(CHST15)
MYD: 102768574(CHST15)
MNA: 107532673(CHST15)
HAI: 109396924(CHST15)
DRO: 112300259(CHST15)
PALE: 102882217(CHST15)
RAY: 107515914(CHST15)
MJV: 108407072(CHST15)
LAV: 100659572(CHST15)
TMU: 101354169
MDO: 100025063(CHST15)
SHR: 100913832(CHST15)
PCW: 110192729(CHST15)
OAA: 100086938(CHST15)
GGA: 423952(CHST15)
MGP: 100542904(CHST15)
CJO: 107316272(CHST15)
NMEL: 110399495(CHST15)
APLA: 101792968(CHST15)
ACYG: 106038980(CHST15)
TGU: 100221434(CHST15)
LSR: 110468429(CHST15)
SCAN: 103813773(CHST15)
GFR: 102041118(CHST15)
FAB: 101819890(CHST15)
PHI: 102110600(CHST15)
PMAJ: 107207093(CHST15)
CCAE: 111931391(CHST15)
CCW: 104694538(CHST15)
ETL: 114061005(CHST15)
FPG: 101915195(CHST15)
FCH: 102046885(CHST15)
CLV: 102094121(CHST15)
EGZ: 104128314(CHST15)
NNI: 104022090(CHST15)
ACUN: 113481638(CHST15)
PADL: 103918531(CHST15)
AAM: 106494366(CHST15)
ASN: 102381919(CHST15)
AMJ: 102565249(CHST15)
PSS: 102454589(CHST15)
CMY: 102936402(CHST15)
CPIC: 101935858(CHST15)
ACS: 100557550(chst15)
PVT: 110070375(CHST15)
PBI: 103065969(CHST15)
PMUR: 107293685(CHST15)
TSR: 106542215(CHST15)
PMUA: 114597401(CHST15)
GJA: 107123681(CHST15)
XLA: 108696098(chst15.L) 108697373
XTR: 100487411(chst15)
NPR: 108784782(CHST15)
DRE: 100537640 565523(chst15)
SANH: 107663285 107674388(chst15)
IPU: 108273603(chst15)
PHYP: 113527444(chst15)
EEE: 113581575(chst15) 113591890
TRU: 101062081 101075284(chst15)
LCO: 104925926(chst15) 104929338
ONL: 100691611(chst15) 100706438
OLA: 101167818 101174629(chst15)
XMA: 102220050(chst15) 102227019
XCO: 114137376 114152214(chst15)
PRET: 103476751 103481828(chst15)
CVG: 107090219(chst15) 107091341
NFU: 107375978 107387792(chst15)
POV: 109626592(chst15) 109630365
LCF: 108899055(chst15)
SLAL: 111660226(chst15) 111663760
HCQ: 109510229 109513397(chst15)
BPEC: 110165469(chst15)
MALB: 109962329 109962953(chst15)
OTW: 112253074 112254813(chst15)
SALP: 111952052 111977451(chst15)
ELS: 105009432(chst15)
SFM: 108932796 108939475(chst15)
PKI: 111853711(chst15)
LCM: 102359407(CHST15)
CMK: 103185442(chst15)
RTP: 109936457(chst15)
PVM: 113823241
OBI: 106868895
 » show all
Reference
1  [PMID:10871629]
  Authors
Ito Y, Habuchi O.
  Title
Purification and characterization of N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase from the squid cartilage.
  Journal
J Biol Chem 275:34728-36 (2000)
DOI:10.1074/jbc.M909633199
  Sequence
Reference
2  [PMID:11572857]
  Authors
Ohtake S, Ito Y, Fukuta M, Habuchi O
  Title
Human N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase cDNA is related to human B cell recombination activating gene-associated gene.
  Journal
J Biol Chem 276:43894-900 (2001)
DOI:10.1074/jbc.M104922200
  Sequence
[hsa:51363] [ag:BAB72146]
Other DBs
ExplorEnz - The Enzyme Database: 2.8.2.33
IUBMB Enzyme Nomenclature: 2.8.2.33
ExPASy - ENZYME nomenclature database: 2.8.2.33
BRENDA, the Enzyme Database: 2.8.2.33
CAS: 242469-38-1

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