KEGG   ENZYME: 2.8.3.24Help
Entry
EC 2.8.3.24                 Enzyme                                 

Name
(R)-2-hydroxy-4-methylpentanoate CoA-transferase;
hadA (gene name)
Class
Transferases;
Transferring sulfur-containing groups;
CoA-transferases
BRITE hierarchy
Sysname
4-methylpentanoyl-CoA:(R)-2-hydroxy-4-methylpentanoate CoA-transferase
Reaction(IUBMB)
4-methylpentanoyl-CoA + (R)-2-hydroxy-4-methylpentanoate = 4-methylpentanoate + (R)-2-hydroxy-4-methylpentanoyl-CoA [RN:R11462]
Reaction(KEGG)
Substrate
4-methylpentanoyl-CoA [CPD:C21400];
(R)-2-hydroxy-4-methylpentanoate [CPD:C03264]
Product
4-methylpentanoate [CPD:C21399];
(R)-2-hydroxy-4-methylpentanoyl-CoA [CPD:C21089]
Comment
The enzyme, characterized from the bacterium Peptoclostridium difficile, participates in an L-leucine fermentation pathway. The reaction proceeds via formation of a covalent anhydride intermediate between a conserved aspartate residue and the acyl group of the CoA thioester substrate.
History
EC 2.8.3.24 created 2016
Orthology
K20882  (R)-2-hydroxy-4-methylpentanoate CoA-transferase
Genes
CBO: CBO2199(hadA)
CBA: CLB_2137(fldA)
CBH: CLC_2142
CBY: CLM_2400(hadA)
CBL: CLK_1638
CBB: CLD_2381
CBI: CLJ_B2407
CBN: CbC4_0485
CBF: CLI_2243
CBM: CBF_2228
CTYK: CTK_C06840(fldA)
CDF: CD630_03950(hadA)
PDC: CDIF630_00523(hadA)
CDC: CD196_0380(hadA)
CDL: CDR20291_0366(hadA)
PDF: CD630DERM_03950(hadA)
 » show all
Taxonomy
Reference
1  [PMID:16957230]
  Authors
Kim J, Darley D, Selmer T, Buckel W
  Title
Characterization of (R)-2-hydroxyisocaproate dehydrogenase and a family III coenzyme A transferase involved in reduction of L-leucine to isocaproate by Clostridium difficile.
  Journal
Appl Environ Microbiol 72:6062-9 (2006)
DOI:10.1128/AEM.00772-06
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.8.3.24
IUBMB Enzyme Nomenclature: 2.8.3.24
ExPASy - ENZYME nomenclature database: 2.8.3.24
BRENDA, the Enzyme Database: 2.8.3.24

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