KEGG   ENZYME: 2.8.3.24
Entry
EC 2.8.3.24                 Enzyme                                 
Name
(R)-2-hydroxy-4-methylpentanoate CoA-transferase;
hadA (gene name)
Class
Transferases;
Transferring sulfur-containing groups;
CoA-transferases
Sysname
4-methylpentanoyl-CoA:(R)-2-hydroxy-4-methylpentanoate CoA-transferase
Reaction(IUBMB)
4-methylpentanoyl-CoA + (R)-2-hydroxy-4-methylpentanoate = 4-methylpentanoate + (R)-2-hydroxy-4-methylpentanoyl-CoA [RN:R11462]
Reaction(KEGG)
R11462
Substrate
4-methylpentanoyl-CoA [CPD:C21400];
(R)-2-hydroxy-4-methylpentanoate [CPD:C03264]
Product
4-methylpentanoate [CPD:C21399];
(R)-2-hydroxy-4-methylpentanoyl-CoA [CPD:C21089]
Comment
The enzyme, characterized from the bacterium Peptoclostridium difficile, participates in an L-leucine fermentation pathway. The reaction proceeds via formation of a covalent anhydride intermediate between a conserved aspartate residue and the acyl group of the CoA thioester substrate.
History
EC 2.8.3.24 created 2016
Orthology
K20882  (R)-2-hydroxy-4-methylpentanoate CoA-transferase
Genes
CBOCBO2199(hadA)
CBACLB_2137(fldA)
CBHCLC_2142
CBYCLM_2400(hadA)
CBLCLK_1638
CBBCLD_2381
CBICLJ_B2407
CBNCbC4_0485
CBFCLI_2243
CBMCBF_2228
CBJH04402_02208
CLDCLSPO_c22310
CTYKCTK_C06840(fldA)
CPRFK7H06_13630
PBIFKXZ80_01460
CDFCD630_03950(hadA)
PDCCDIF630_00523(hadA)
CDCCD196_0380(hadA)
CDLCDR20291_0366(hadA)
PDFCD630DERM_03950(hadA)
PSORRSJ16_16055
LASAL9O85_02225
 » show all
Reference
1  [PMID:16957230]
  Authors
Kim J, Darley D, Selmer T, Buckel W
  Title
Characterization of (R)-2-hydroxyisocaproate dehydrogenase and a family III coenzyme A transferase involved in reduction of L-leucine to isocaproate by Clostridium difficile.
  Journal
Appl Environ Microbiol 72:6062-9 (2006)
DOI:10.1128/AEM.00772-06
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.8.3.24
IUBMB Enzyme Nomenclature: 2.8.3.24
ExPASy - ENZYME nomenclature database: 2.8.3.24
BRENDA, the Enzyme Database: 2.8.3.24

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