Entry
Name
tRNA-2-methylthio-N6-dimethylallyladenosine synthase;
MiaB;
2-methylthio-N-6-isopentenyl adenosine synthase;
tRNA-i6A37 methylthiotransferase;
tRNA (N6-dimethylallyladenosine37):sulfur-(sulfur carrier),S-adenosyl-L-methionine C2-methylthiotransferase
Class
Transferases;
Transferring sulfur-containing groups;
Transferring alkylthio groups
BRITE hierarchy
Sysname
tRNA N6-(3-methylbut-2-en-1-yl)-adenine37:sulfur-(sulfur carrier),S-adenosyl-L-methionine C2-(methylsulfanyl)transferase
Reaction(IUBMB)
N6-(3-methylbut-2-en-1-yl)-adenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenine + electron acceptor (overall reaction) [RN:
R10645 ];
(1a) N6-(3-methylbut-2-en-1-yl)-adenine37 in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced electron acceptor = N6-(3-methylbut-2-en-1-yl)-2-thioadenine37 in tRNA + (sulfur carrier) + L-methionine + 5'-deoxyadenine + electron acceptor [RN:
R10646 ];
(1b) S-adenosyl-L-methionine + N6-(3-methylbut-2-en-1-yl)-2-thioadenine37 in tRNA = S-adenosyl-L-homocysteine + N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenine37 in tRNA [RN:
R10647 ]
Reaction(KEGG)
Substrate
N6-(3-methylbut-2-en-1-yl)-adenine37 in tRNA;
sulfur-(sulfur carrier);
S-adenosyl-L-methionine [CPD:
C00019 ];
reduced electron acceptor;
N6-(3-methylbut-2-en-1-yl)-2-thioadenine37 in tRNA
Product
N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenine37 in tRNA;
S-adenosyl-L-homocysteine [CPD:
C00021 ];
(sulfur carrier);
L-methionine [CPD:
C00073 ];
5'-deoxyadenine;
electron acceptor;
N6-(3-methylbut-2-en-1-yl)-2-thioadenine37 in tRNA
Comment
This bacterial enzyme binds two [4Fe-4S] clusters as well as the transferred sulfur [3]. The enzyme is a member of the superfamily of S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes. The sulfur donor is believed to be one of the [4Fe-4S] clusters, which is sacrificed in the process, so that in vitro the reaction is a single turnover. The identity of the electron donor is not known.
History
EC 2.8.4.3 created 2014, modified 2015
Orthology
K06168 tRNA-2-methylthio-N6-dimethylallyladenosine synthase
Genes
ECOO : ECRM13514_0686(yleA)
ECOH : ECRM13516_0632(yleA)
ECW : EcE24377A_0690(miaB)
EDJ : ECDH1ME8569_0630(miaB)
ECOI : ECOPMV1_00671(miaB)
REE : electrica_03645(miaB)
RTG : NCTC13098_05342(miaB)
CLAP : NCTC11466_03291(miaB)
KIE : NCTC12125_02320(miaB)
AHN : NCTC12129_03539(miaB)
METY : MRY16398_40720(miaB)
PSHI : SAMEA2665130_2231(miaB)
MPHG : MPHASIOC01_003925(miaB)
YPG : YpAngola_A1829(miaB)
YPI : YpsIP31758_2915(miaB)
SMW : SMWW4_v1c11880(miaB)
SFJ : SAMEA4384070_1269(miaB)
SOF : NCTC11214_05713(miaB)
SSAR : SSARUM_001124(miaB)
PAQU : DMB82_0014505(miaB)
PARL : PEC302110_11840(miaB)
BAPF : BUMPF009_CDS00158(ylea)
BAPG : BUMPG002_CDS00158(ylea)
BAPU : BUMPUSDA_CDS00158(ylea)
BAPW : BUMPW106_CDS00158(ylea)
TPTY : NCTC11468_01170(miaB)
PHEI : NCTC12003_02969(miaB)
PRJ : NCTC6933_01068(miaB)
LRI : NCTC12151_02231(miaB)
HIC : NTHIC486_01024(miaB)
HPIT : NCTC13334_01132(miaB)
HHZ : NCTC10839_01059(miaB)
HAEG : NCTC8502_01955(miaB)
PDAG : 4362423_01250(miaB)
ADP : NCTC12871_00169(miaB)
ALIG : NCTC10568_01641(miaB)
ASEG : NCTC10977_01894(miaB)
AVT : NCTC3438_01080(miaB)
RPNE : NCTC8284_03181(miaB_2)
PAET : NCTC13378_00320(miaB)
XFU : XFF4834R_chr24450(miaB)
XPH : XppCFBP6546_00810(miaB)
XHD : LMG31886_18360(miaB)
SACZ : AOT14_13960(rimO_1)
LLZ : LYB30171_00877(miaB)
VAU : VANGNB10_cI0837c(miaB)
VPL : SA104470976_01806(miaB)
VIB : VspSTUT11_22670(miaB)
VIS : VspSTUT16_23910(miaB)
PVD : CFBP1590__4689(miaB)
PPRC : PFLCHA0_c54020(miaB)
PMUD : NCTC8068_04624(miaB)
PFW : PF1751_v1c48200(miaB)
PPUU : PputUW4_04763(miaB)
PTRT : HU722_0025935(miaB)
PTK : EXN22_10140 EXN22_24180(miaB)
PTW : TUM18999_09850(miaB)
PTAE : NCTC10697_00639(miaB)
MSHE : MAALD49_27150(miaB)
PSYC : DABAL43B_2344(miaB)
MCAT : MC25239_01397(miaB)
MCUN : NCTC10297_00723(miaB)
SCAA : TUM17387_09970(miaB)
SALH : HMF8227_00994(miaB)
CBS : COXBURSA331_A0683(miaB)
LLG : 44548918_01025(miaB)
LJR : NCTC11533_01211(miaB)
LCJ : NCTC11976_01995(miaB)
LWA : SAMEA4504053_1511(miaB)
LSS : NCTC12082_00741(miaB)
LADL : NCTC12735_01197(miaB)
LANT : TUM19329_16310(miaB)
MISZ : MishRS11D_35790(miaB)
TIB : THMIRHAM_18130(miaB)
TSE : THMIRHAS_20960(miaB)
TZO : THMIRHAT_18440(miaB)
RHEI : ATY27_10735 ATY27_18920
TFRI : Thiofri_04518(miaB)
TNI : TVNIR_2544(miaB_[H])
GAI : IMCC3135_10265(miaB)
THIC : TspCOW1_16780(miaB)
HAAH : HALA3H3_710044(miaB)
HAXI : HAALTHF_46510n(miaB)
ALCA : ASALC70_03388(miaB_1)
AEL : NCTC12917_01076(miaB)
CHJ : NCTC10426_00282(miaB)
CDIZ : CEDIAZO_02439(miaB_2)
NMM : NMBM01240149_0323(miaB)
NMS : NMBM01240355_1796(miaB)
NMQ : NMBM04240196_1804(miaB)
NMZ : NMBNZ0533_0461(miaB)
NMX : NMA510612_0650(miaB)
NWE : SAMEA3174300_1955(miaB)
NZO : SAMEA4504057_0016(miaB)
NCI : NCTC10296_01087(miaB)
NCZ : NCTC10294_01326(miaB)
NANI : NCTC12227_00102(miaB)
ECOR : SAMEA4412678_0045(miaB)
IOD : EJO50_03820 EJO50_15310(miaB)
BMV : BMASAVP1_A2720(miaB)
BML : BMA10229_A2358(miaB)
BPM : BURPS1710b_0889(miaB)
BPL : BURPS1106A_0724(miaB)
POLT : TUM22923_15980(miaB)
PLG : NCTC10937_04767(miaB)
LMIR : NCTC12852_02421(miaB)
BUO : BRPE64_ACDS04240(miaB)
BUE : BRPE67_ACDS03990(miaB)
BTRM : SAMEA390648702138(miaB)
AXX : ERS451415_04998(miaB)
LIM : L103DPR2_02719(miaB)
LIH : L63ED372_02471(miaB)
SMIO : CATMQ487_12660(miaB)
SDR : SCD_n00241 SCD_n02591(miaB)
URU : DSM104443_00385(miaB)
UPL : DSM104440_00450(miaB)
THAG : CKCBHOJB_00594(miaB)
FPHO : SHINM1_000820(miaB)
APLT : ANPL_03070(miaB_1) ANPL_03110(miaB_2)
MESU : LHFGNBLO_000305(miaB)
RBS : RHODOSMS8_03203(miaB)
SMEL : SM2011_c01115(miaB)
SFD : USDA257_c00410(miaB)
SAME : SAMCFNEI73_Ch0415(miaB)
AGR : AGROH133_03545(miaB)
ALF : CFBP5473_14175(miaB)
AVV : RvVAT039_20090(miaB)
AVF : RvVAR031_07240(miaB)
ASAL : CFBP5507_00160(miaB)
RPUS : CFBP5875_00160(miaB)
ALEG : CFBP4996_00160(miaB)
REL : REMIM1_CH00382(miaB)
REP : IE4803_CH00404(miaB)
REI : IE4771_CH00413(miaB)
RTR : RTCIAT899_CH02415(miaB)
RGA : RGR602_CH00405(miaB)
RPHA : AMC79_CH00417(miaB)
RLW : RlegWSM1455_00100(miaB)
RBW : RLCC275e_00105(miaB)
NEN : NCHU2750_00420(miaB)
LAS : CLIBASIA_01565(miaB)
BRAD : BF49_0031 BF49_7356
BSEP : HAP48_0016335(miaB)
BCAN : BcanWSM471_00390(miaB)
VGO : GJW-30_1_00550(miaB)
BBK : BARBAKC583_1243(miaB)
BEZ : NCTC12898_00117(miaB_1)
MAQU : Maq22A_1p32120(miaB)
MMES : MMSR116_27715(miaB)
MTUN : MTUNDRAET4_2485(miaB)
FIL : BN1229_v1_2221(miaB)
FIY : BN1229_v1_2220(miaB)
BVY : NCTC9239_03313(miaB)
TSV : DSM104635_03822(miaB)
PHP : PhaeoP97_02884(miaB)
PPIC : PhaeoP14_00389(miaB)
SINL : DSM14862_02335(miaB)
SPSE : SULPSESMR1_02301(miaB)
RMM : ROSMUCSMR3_00933(miaB)
AHT : ANTHELSMS3_01875(miaB)
LALG : LentiSH36_00748(miaB)
LVS : LOKVESSMR4R_02226(miaB)
AALK : LGT41_0006625(miaB)
SBIN : SBA_ch1_26170(miaB)
SPAG : sphantq_03634(miaB)
SFLA : SPHFLASMR4Y_00689(miaB)
EBLA : JGUZn3_18550(miaB_2)
HPO : HMPREF4655_20511(miaB)
HPX : HMPREF0462_0322(miaB)
HCL : NCTC13205_01105(miaB_2)
HSH : NHP194022_14420(miaB)
HPUL : NCTC13154_00174(miaB_1)
SULG : FJR48_09590(miaB) FJR48_11900(miaB) FJR48_12120(miaB)
CJV : MTVDSCj20_0431(miaB)
CFV : CFVI03293_1415(miaB)
CAMP : CFT03427_1340(miaB)
CCOC : CCON33237_0867(miaB)
CLR : UPTC16701_0327(miaB)
CLM : UPTC16712_0330(miaB)
CAMR : CAQ16704_0332(miaB)
CHV : CHELV3228_0827(miaB)
CPIN : CPIN18020_1181(miaB)
CAMZ : CVIC12175_0378(miaB)
GEC : GEO60473_07530(miaB)
GBN : GEOBRER4_07660(miaB)
PPOR : JCM14722_03640(miaB)
SBF : JCM31447_06140(miaB)
OBG : Verru16b_01097(miaB)
ABIW : Abiwalacus_09370(miaB)
LCRE : Pla8534_54720(miaB)
PBU : L21SP3_02213(miaB_2)
PBAP : Pla133_30710(miaB_2)
LKB : LPTSP3_g16990(miaB_2)
PSPC : Strain318_001304(miaB)
BTHO : Btheta7330_05111(miaB)
BCEL : BcellWH2_02868(miaB)
BFC : BacF7301_05075(miaB)
PCRE : NCTC12858_00203(miaB_1)
PCAG : NCTC12856_00328(miaB_1)
PSOE : CE91St14_04130(miaB)
COPR : Cop2CBH44_22720(miaB)
CFAS : Cfast33896_17580(miaB)
PMZ : HMPREF0659_A6396(miaB)
PDN : HMPREF9137_0531(miaB)
PHER : prwr041_10570(miaB)
POC : NCTC13071_00546(miaB_1)
ACOU : A5CBH24_19760(miaB)
ADA : A5CPEGH6_19410(miaB)
MCOS : GM418_06885(miaB) GM418_20460(miaB)
MBAS : ALGA_2274 ALGA_4010
CBAM : PIECOFPK_02605(miaB)
SMIZ : 4412673_03660(miaB_2)
SDJ : NCTC13534_05079(miaB_2)
STHA : NCTC11429_01127(miaB_1)
MGOT : MgSA37_04325(miaB_1)
PSEZ : HME7025_01386(miaB)
FOK : KK2020170_11540(miaB)
PHG : KCTC32516_00334(miaB)
AALG : AREALGSMS7_00711(miaB)
KOS : KORDIASMS9_00441(miaB)
KAN : IMCC3317_23290(miaB)
SPON : HME9304_00975(miaB)
OHM : MSHRCOH1_07520(miaB)