Entry
Name
tRNA (N6-L-threonylcarbamoyladenosine37-C2)-methylthiotransferase;
MtaB;
methylthio-threonylcarbamoyl-adenosine transferase B;
CDKAL1 (gene name);
tRNA (N6-L-threonylcarbamoyladenosine37):sulfur-(sulfur carrier),S-adenosyl-L-methionine C2-methylthiotransferase
Class
Transferases;
Transferring sulfur-containing groups;
Transferring alkylthio groups
BRITE hierarchy
Sysname
tRNA (N6-L-threonylcarbamoyladenosine37):sulfur-(sulfur carrier),S-adenosyl-L-methionine C2-(methylsulfanyl)transferase
Reaction(IUBMB)
N6-L-threonylcarbamoyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-(methylsulfanyl)-N6-L-threonylcarbamoyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor (overall reaction) [RN:
R10649 ];
(1a) N6-L-threonylcarbamoyladenine37 in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced electron acceptor = 2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor [RN:
R10650 ];
(1b) S-adenosyl-L-methionine + 2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA = S-adenosyl-L-homocysteine + 2-(methylsulfanyl)-N6-L-threonylcarbamoyladenine37 in tRNA [RN:
R10651 ]
Reaction(KEGG)
Substrate
N6-L-threonylcarbamoyladenine37 in tRNA;
sulfur-(sulfur carrier);
S-adenosyl-L-methionine [CPD:
C00019 ];
reduced electron acceptor;
2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA
Product
2-(methylsulfanyl)-N6-L-threonylcarbamoyladenine37 in tRNA;
S-adenosyl-L-homocysteine [CPD:
C00021 ];
(sulfur carrier);
L-methionine [CPD:
C00073 ];
5'-deoxyadenosine [CPD:
C05198 ];
electron acceptor;
2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA
Comment
The enzyme, which is a member of the S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes superfamily, binds two [4Fe-4S] clusters as well as the transferred sulfur. The sulfur donor is believed to be one of the [4Fe-4S] clusters, which is sacrificed in the process, so that in vitro the reaction is a single turnover. The identity of the electron donor is not known.
History
EC 2.8.4.5 created 2014, modified 2015
Orthology
K15865 threonylcarbamoyladenosine tRNA methylthiotransferase CDKAL1
K18707 threonylcarbamoyladenosine tRNA methylthiotransferase MtaB
Genes
MNA : 107533701 107539630(CDKAL1)
SANH : 107663002 107663797(cdkal1)
SGH : 107558653(cdkal1) 107573107
CAUA : 113076577(cdkal1) 113092497 113101113 113116593
MASI : 127452731 127454388
TNG : GSTEN00018271G001 GSTEN00018272G001
OMM : 135519854(cdkal1) 135519856
DSI : Dsimw501_GD25482(Dsim_GD25482)
AMER : 121592816 121602433
APLN : 108743519 112905981
CFEL : 113366162 113366204
RSAN : 119371663 119371678
DPTE : 113794258 113795298
CEL : CELE_Y92H12BL.1(Y92H12BL.1)
CSAT : 104701876 104712787 104753789
HSYR : 120180713 120192913
MSYL : 126602249 126632701
SSPL : 121805796 121806933
PSOM : 113283398 113356570
TAES : 123045496 123053344 123189245
PVIR : 120641024 120682369
SMO : SELMODRAFT_31574 SELMODRAFT_424054 SELMODRAFT_424158 SELMODRAFT_446559 SELMODRAFT_52269
SSAO : 94299987(SS50377_25964)
TFRI : Thiofri_03780(mtaB)
GAI : IMCC3135_11590(mtaB)
MESU : LHFGNBLO_001133(mtaB)
RBS : RHODOSMS8_02886(mtaB)
ALF : CFBP5473_01925(mtaB)
ASAL : CFBP5507_12270(mtaB)
RPUS : CFBP5875_11900(mtaB)
ALEG : CFBP4996_12295(mtaB)
RLW : RlegWSM1455_20410(mtaB)
RBW : RLCC275e_20925(mtaB)
NEN : NCHU2750_34840(mtaB)
BSEP : HAP48_0014290(mtaB)
BCAN : BcanWSM471_01945(mtaB)
VGO : GJW-30_1_01268(mtaB)
BEZ : NCTC12898_01610(miaB_2)
MAQU : Maq22A_c07140(miaB)
MMES : MMSR116_13005(mtaB)
MTUN : MTUNDRAET4_2869(mtaB)
FIL : BN1229_v1_0879(mtaB)
FIY : BN1229_v1_0884(mtaB)
TSV : DSM104635_02213(mtaB)
SINL : DSM14862_00207(mtaB)
SPSE : SULPSESMR1_03008(mtaB)
RMM : ROSMUCSMR3_03083(mtaB)
AHT : ANTHELSMS3_00627(mtaB)
LVS : LOKVESSMR4R_03640(mtaB)
AALK : LGT41_0007225(mtaB)
SPAG : sphantq_02717(mtaB)
SFLA : SPHFLASMR4Y_00867(mtaB)
GEC : GEO60473_04020(mtaB)
GBN : GEOBRER4_25100(mtaB)
DOV : DSCO28_16550 DSCO28_19270(mtaB)
LCRE : Pla8534_44890(mtaB)
VAI : BU251_05135 BU251_08655
TRC : DYE49_00385 DYE49_06830
LKB : LPTSP3_g08380(miaB_1)
BTHO : Btheta7330_00888(mtaB)
BCEL : BcellWH2_01618(mtaB)
BFC : BacF7301_00050(mtaB)
PCRE : NCTC12858_00305(miaB_2)
PCAG : NCTC12856_01652(miaB_2)
CFAS : Cfast33896_20180(mtaB)
PMZ : HMPREF0659_A6536(yqeV)
PDN : HMPREF9137_1838(yqeV)
POC : NCTC13071_01475(miaB_2)
MBAS : ALGA_0991 ALGA_4011
CBAM : PIECOFPK_01103(mtaB)
SMIZ : 4412673_01137(miaB_1)
SDJ : NCTC13534_01611(miaB_1)
STHA : NCTC11429_02705(miaB_2)
PSEZ : HME7025_01511(mtaB)
POA : CW731_03960 CW731_06195
PHG : KCTC32516_00384(mtaB_1) KCTC32516_01739(mtaB_2)
AALG : AREALGSMS7_03927(mtaB)
KOS : KORDIASMS9_04071(mtaB)
KAN : IMCC3317_43080(mtaB)
SPON : HME9304_00082(mtaB)
OHM : MSHRCOH1_06125(mtaB)
CGLE : NCTC11432_04235(miaB_2)
CTAI : NCTC12078_01438(miaB_1)
CTAK : 4412677_02388(miaB_2)
CHRJ : CHRYMOREF3P_3761(mtaB)
CJG : NCTC13459_01025(miaB_1)
CANT : NCTC13489_01040(miaB_1)
KFA : Q73A0000_01270(mtaB)
SMUM : SMML_v1c00890(miaB)
PTAN : CRYO30217_01546(mtaB)
PROC : Ptc2401_01888(mtaB)
PROW : OO006_02670(mtaB) OO006_03045
PROI : OO005_02630(mtaB) OO005_02955
NVA : G3M78_11400 G3M78_13585(mtaB)
BAZ : BAMTA208_13045(rimO)
BSON : S101395_01798(mtaB)
BFD : NCTC4823_01787(rimO)
BACO : OXB_0355 OXB_2952 OXB_3241
BLEN : NCTC4824_01648(rimO)
PARG : PspKH34_10260(mtaB)
SUQ : HMPREF0772_11564(yqeV)
SDP : NCTC12225_01430(miaB_1)
SPIC : SAMEA4384060_1370(miaB_1)
SSH : NCTC13712_01573(miaB_2)
SSIM : SAMEA4384339_1467(miaB_2)
SSTE : SAMEA4384403_1184(miaB_1)
MCAK : MCCS_15180(mtaB_1) MCCS_15570(mtaB_2)
BAYD : BSPP4475_13095(mtaB)
COHN : KCTCHS21_41470(mtaB)
CCB : Clocel_1441 Clocel_3128
CCOH : SAMEA4530647_1794(miaB_2)
HHW : NCTC503_01642(miaB_2)
CLO : HMPREF0868_0047(yqeV)
DKU : Desku_0018 Desku_2656
BPRO : PMF13cell1_02078(mtaB)
BLUI : Blut17040_24360(mtaB)
CSCI : HDCHBGLK_03577(mtaB)
CBAR : PATL70BA_3421(mtaB)
AMIC : Ami3637_00630(mtaB)
ABUT : Ami103574_07865(mtaB)
PHX : KGNDJEFE_01849(mtaB)
FAA : HMPREF0389_00081(yqeV)
NTH : Nther_1187 Nther_1807
NCD : ACONDI_00622(mtaB_1) ACONDI_01600(mtaB_2)
PHAR : NCTC13077_00968(miaB_2)
PIV : NCTC13079_00578(miaB_1)
VRM : 44547418_00902(miaB_2)
VDN : NCTC11831_01060(miaB_1)
SELM : TAMA11512_22680(mtaB)
MANA : MAMMFC1_03481(mtaB)
PFAC : PFJ30894_01333(mtaB)
FIT : Fi14EGH31_23960(mtaB)
PCAT : Pcatena_07560(miaB_1)
RTS : CE91St31_17600(mtaB)
FPOL : ERS445057_00982(miaB_1)
MEHF : MmiHf6_10740(miaB_1)
MEES : MmiEs2_16420(miaB_2)
TVO : TVG1411343(TVG1411343)
SHC : Shell_1336 Shell_1367
DFD : Desfe_0149 Desfe_1197
DMU : Desmu_0231 Desmu_1097
THG : TCELL_0167 TCELL_0391
LOKI : Lokiarch_30780(miaB_2) Lokiarch_46780(miaB_3)
» show all
Taxonomy
Reference
Authors
Arragain S, Handelman SK, Forouhar F, Wei FY, Tomizawa K, Hunt JF, Douki T, Fontecave M, Mulliez E, Atta M
Title
Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.8.4.5
ExPASy - ENZYME nomenclature database: 2.8.4.5
BRENDA, the Enzyme Database: 2.8.4.5