KEGG ENZYME: 3.1.1.103

ENZYME: 3.1.1.103

Entry

EC 3.1.1.103                Enzyme

Name

teichoic acid D-alanine hydrolase;
fmtA (gene name)

Class

Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases

Sysname

teichoic acid D-alanylhydrolase

Reaction(IUBMB)

[(4-D-Ala)-(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-beta-D-ManNAc-(1->4)-alpha-D-GlcNAc-P-peptidoglycan + n H2O = [(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-beta-D-ManNAc-(1->4)-alpha-D-GlcNAc-P-peptidoglycan + n D-alanine [RN:R11965]

Reaction(KEGG)

R11965

Substrate

[(4-D-Ala)-(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-beta-D-ManNAc-(1->4)-alpha-D-GlcNAc-P-peptidoglycan;
H2O [CPD:C00001]

Product

[(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-beta-D-ManNAc-(1->4)-alpha-D-GlcNAc-P-peptidoglycan;
D-alanine [CPD:C00133]

Comment

The enzyme, characterized from the bacterium Staphylococcus aureus, removes D-alanine groups from the teichoic acid produced by this organism, thus modulating the electrical charge of the bacterial surface. The activity greatly increases methicillin resistance in MRSA strains.

History

EC 3.1.1.103 created 2018

Orthology

K22580

teichoic acid D-alanine hydrolase

Genes

SDO:	SD1155_07555

SRD:	SD10_11505

MYR:	MYRA21_2893

MPW:

MPR_1758

MOD:	AS202_14180

PAAE:

NPX36_09960

EAO:

BD94_0966

EGO:	BBD34_13620

CHH:	A0O34_16270

CNK:	EG343_06155

BCA:	BCE_3507 BCE_4110

BCR:	BCAH187_A3518 BCAH187_A4174

BCB:	BCB4264_A3498

BCQ:	BCQ_3295(bla) BCQ_3833

BNC:	BCN_3307 BCN_3955

BCER:

BCK_14985 BCK_17770

BCEF:

BcrFT9_02665 BcrFT9_03155

BCY:	Bcer98_2742

BTB:	BMB171_C3163 BMB171_C3707 BMB171_P0160

BTT:	HD73_3735 HD73_4342

BTHR:

YBT1520_18415 YBT1520_21525

BTHI:

BTK_18405 BTK_21375

BTC:	CT43_CH4049

BTF:	YBT020_17115 YBT020_19905

BTM:

MC28_2645

BTG:	BTB_c41780(pbpX3)

BTHT:

H175_ch4115

BTHU:

YBT1518_22395

BTHY:

AQ980_09635 AQ980_12640

BWE:	BcerKBAB4_3210 BcerKBAB4_3872

BWW:	bwei_0908 bwei_1441

BMYO:

BG05_1979 BG05_2505

BTY:	Btoyo_0712

BMYC:

DJ92_1138

BBY:	CY96_19305

BWD:	CT694_21755

BMOB:

MLA2C4_18155

BNT:	GSN03_19160

BPAC:

LMD38_06870

BPAN:

NLJ82_19650

BAAP:

BGI23_19425

LPAK:

GDS87_11575

VIG:	BKP57_06435

SAU:	SA0909(fmtA)

SAV:	SAV1057(fmt)

SAW:	SAHV_1049(fmt)

SAH:	SaurJH1_1139

SAJ:	SaurJH9_1116

SAM:	MW0940(fmt)

SAS:

SAS0992

SAR:	SAR1030(fmt)

SAC:

SACOL1066

SAX:	USA300HOU_1001(fmt1)

SAA:	SAUSA300_0959(fmt)

SAO:	SAOUHSC_00998

SAE:

NWMN_0926

SAD:

SAAV_1021

SUU:	M013TW_0988(fmtA)

SUV:	SAVC_04450

SUE:

SAOV_1003

SUJ:	SAA6159_00913(fmtA)

SUK:	SAA6008_01012

SUC:

ECTR2_912

SUT:	SAT0131_01092

SUQ:	HMPREF0772_12177

SUZ:	MS7_1014(fmtA)

SUD:	ST398NM01_1054

SUX:	SAEMRSA15_08880(fmt)

SUW:	SATW20_10530(fmt)

SUG:

SAPIG1054

SUF:	SARLGA251_09710(fmt)

SAUA:

SAAG_02168

SAUE:

RSAU_000944(fmtA)

SAUN:

SAKOR_00978

SAUS:

SA40_0927(fmt)

SAUU:

SA957_0942(fmt)

SAUG:

SA268_0945(fmt)

SAUZ:

SAZ172_0997(fmtA)

SAUT:

SAI1T1_2007480

SAUJ:

SAI2T2_1007500

SAUK:

SAI3T3_1007490

SAUQ:

SAI4T8_1007480

SAUV:

SAI7S6_1007490(fmtA)

SAUW:

SAI5S5_1007450(fmtA)

SAUX:

SAI6T6_1007460(fmtA)

SAUY:

SAI8T7_1007490(fmtA)

SAUF:

X998_1056

SAB:	SAB0923(fmt)

SUY:	SA2981_1011(fmtA)

SAUB:

C248_1082(fmt)

SAUM:

BN843_9630

SAUC:

CA347_973(fmtA)

SAUR:

SABB_01023(fmtA)

SAUI:

AZ30_05045

SAUD:

CH52_00600

SAMS:

NI36_05060

SUH:	SAMSHR1132_09050

SER:

SERP0641

SEP:

SE_0754

SEPP:

SEB_00730

SEPS:

DP17_2087(fmtA)

SHA:	SH1907(fmt)

SHH:	ShL2_01771

SSP:

SSP1736

SCA:

SCA_0665

SLG:	SLGD_01819

SLN:	SLUG_18150(fmt)

SSD:	SPSINT_0518

SDT:	SPSE_1942(fmtA)

SDP:	NCTC12225_02173(fmtA)

SWA:	A284_08550

SPAS:

STP1_2114

SXY:	BE24_02600

SXL:	SXYLSMQ121_1737

SXO:	SXYL_01880(fmtA)

SHU:	SHYC_01895

SCAP:

AYP1020_0379(pbpX)

SSCH:

LH95_09295

SSCZ:

RN70_10070

SAGQ:

EP23_08225

SEQO:

SE1039_08140

SSIF:

AL483_06610 AL483_06615

SCV:	A4G25_04610

SLZ:	B5P37_08435

SCOH:

BZ166_00595

SURY:

MUA21_03785

SNL:	BJD96_09640

SKL:	C7J89_10440

SHOM:

EGX58_01415

SCAR:

DWB96_09410

SCHR:

DWB92_01740

SARL:

SAP2_17690

SPIC:

SAMEA4384060_1899(fmtA_2)

SSH:	NCTC13712_01002(fmtA_1)

SSIM:

SAMEA4384339_0968(fmtA_1)

SLLO:

ISP08_08725

SAUL:

I6G39_08025

SSAC:

I6I31_07495

SPSD:

JMB28_09405

STAS:

HYI43_08930

SEDA:

MNY58_09315

SCAQ:

QMO72_09345

SROT:

ML435_04800(fmtA)

STAP:

AOB58_946

SGAI:

K3U27_02865

SSCU:

CEP64_08355

SFF:	FOB90_10230

SSTE:

SAMEA4384403_1803(fmtA)

MLEN:

H3V22_13610

MVT:	I6J10_02355

MARY:

LAU42_03235 LAU42_03240

MPSH:

QA539_03075

MCL:	MCCL_0555 MCCL_0556

MCAK:

MCCS_07670(pbpX_1) MCCS_07680(pbpX_2)

LGZ:	NCTC10812_01209(fmtA_2)

SAG:

SAG0658

SAN:

gbs0640

SAK:

SAK_0786

SGC:

A964_0654

SAGS:

SaSA20_0551

SAGL:

GBS222_0540

SAGM:

BSA_7450

SAGP:

V193_03115

SAGC:

DN94_03115

SAGT:

GBSCOH1_0580

SAGE:

EN72_04025

SAGG:

EN73_03725

SAGN:

W903_0749

SAGJ:

ID870_05980

LJF:	FI9785_1472(pbpX) FI9785_1730

LJH:

LJP_1717c

LJN:	T285_07215 T285_08775

LAC:	LBA0059(pbpX) LBA0858 LBA1006 LBA1605(pbpX)

LAD:	LA14_0059 LA14_0882 LA14_1021 LA14_1596

LAF:	SD55_0058(pbpX1) SD55_0880 SD55_1021 SD55_1605(pbpX2)

LDB:	Ldb1644 Ldb1645

LBU:	LBUL_1523 LBUL_1524

LDE:	LDBND_1553 LDBND_1554

LDL:	LBU_1404 LBU_1405

LGA:

LGAS_1459

LHE:	lhv_0070 lhv_0912 lhv_1103 lhv_1192

LHL:	LBHH_0081 LBHH_0974 LBHH_1106 LBHH_1247

LHR:	R0052_00420 R0052_05750 R0052_06505 R0052_07365

LHV:	lhe_0078 lhe_0876 lhe_1002 lhe_1083

LHH:	LBH_0054 LBH_0761 LBH_0901

LHD:	HUO_01315 HUO_06210 HUO_06715 HUO_07495

LCR:	LCRIS_00057 LCRIS_00552(pbp1) LCRIS_00881 LCRIS_01083(pbpX)

LAM:	LA2_04505 LA2_05270

LAI:	LAC30SC_00255 LAC30SC_04310 LAC30SC_05065

LAY:	LAB52_04290 LAB52_05025

LKE:	WANG_0789 WANG_1631(pbpX)

LAW:	LACWKB8_0097

LAE:	LBAT_0074 LBAT_0258

LGL:	AO203_02730 AO203_07280 AO203_08025 AO203_08705

LAMY:

B1745_00330

LKL:	DKL58_00405

LAPI:

DKL56_00340

LHS:	DLD54_00485

LTA:	H1A07_07225 H1A07_08850

LUU:	H4B44_00405 H4B44_06810

LPAI:

GYM71_00530

LCL:	LOCK919_2316

LPI:	LBPG_02057

LCX:	LCA12A_0680

LRH:

LGG_02141

LRG:

LRHM_2058

LRL:	LC705_02148

LRA:

LRHK_2149

LRO:	LOCK900_2102

LRC:	LOCK908_2211

LRE:

Lreu_1924

LRF:

LAR_1801

LRU:	HMPREF0538_21124

LRT:

LRI_1866

LRR:	N134_10195

LVA:	LV515_05195

LFN:	LF145_07135

LPOR:

PRK60_03620

LNG:	BSQ50_00975

LBH:	Lbuc_0085 Lbuc_0092

LBN:	LBUCD034_0131(pbpX1)

LPAR:

FAM21731_00881 FAM21731_00888

LKF:	DNL43_05595

LHIL:

G8J22_00383

LZY:	LZ3411_1809

LSUA:

H3M12_00105

LPD:	AYR62_06155

PPE:

PEPE_0564

PPEN:

T256_03075

PACI:

A4V11_09625

LSA:

LCA_0648

LCV:	FBA2_03350

LHB:	D1010_12850

LCI:

LCK_00771

WCE:

WS08_0647

WCT:

WS74_0649

WCI:	WS105_0709

WVR:	IE337_04570 IE337_04585

EFC:	EFAU004_01360

EFAU:

EFAU085_00973

EFU:	HMPREF0351_10933

EHR:

EHR_00080

EMU:

EMQU_0916

EDU:

LIU_05530

ETH:	CK496_08425

MPS:

MPTP_1050

MPX:

MPD5_0898

VPI:	BW732_08825

VCP:	H9L18_02050

VHY:	G7082_04090

» show all

Reference

1 [PMID:9371333]

Authors

Komatsuzawa H, Sugai M, Ohta K, Fujiwara T, Nakashima S, Suzuki J, Lee CY, Suginaka H

Title

Cloning and characterization of the fmt gene which affects the methicillin resistance level and autolysis in the presence of triton X-100 in methicillin-resistant Staphylococcus aureus.

Journal

Antimicrob Agents Chemother 41:2355-61 (1997)
DOI:10.1128/AAC.41.11.2355

Sequence

[sau:SA0909]

Reference

2 [PMID:22564846]

Authors

Qamar A, Golemi-Kotra D

Title

Dual roles of FmtA in Staphylococcus aureus cell wall biosynthesis and autolysis.

Journal

Antimicrob Agents Chemother 56:3797-805 (2012)
DOI:10.1128/AAC.00187-12

Reference

3 [PMID:26861022]

Authors

Rahman MM, Hunter HN, Prova S, Verma V, Qamar A, Golemi-Kotra D

Title

The Staphylococcus aureus Methicillin Resistance Factor FmtA Is a d-Amino Esterase That Acts on Teichoic Acids.

Journal

MBio 7:e02070-15 (2016)
DOI:10.1128/mBio.02070-15

Other DBs

ExplorEnz - The Enzyme Database:

3.1.1.103

IUBMB Enzyme Nomenclature:

3.1.1.103

ExPASy - ENZYME nomenclature database:

3.1.1.103

BRENDA, the Enzyme Database:

3.1.1.103

All links

Protein sequence (31219)   
   UniProt (1850)   
   SWISS-PROT (8)   
   RefSeq(pep) (29361)   
DNA sequence (37690)   
   RefSeq(nuc) (26822)   
   GenBank (5553)   
   EMBL (5315)   
Protein domain (3)   
   InterPro (3)   
All databases (68912)   

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